| 1. |
- Björnsdottir, Halla, et al.
(författare)
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Quantification of heterotypic granule fusion in human neutrophils by imaging flow cytometry.
- 2016
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Ingår i: Data in brief. - : Elsevier BV. - 2352-3409. ; 6, s. 386-93
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Tidskriftsartikel (refereegranskat)abstract
- Human neutrophils are filled with intracellular storage organelles, called granules and secretory vesicles, which differ in their content of soluble matrix proteins and membrane-bound molecules. To date, at least four distinct granule/vesicle subsets have been identified. These organelles may secrete their content extracellularly following mobilization to and fusion with the plasma membrane, but some of them may also fuse with internal membrane-enclosed organelles, typically a plasma membrane-derived phagosome. There are also instances where different granules appear to fuse with one another, a process that would enable mixing of their matrix and membrane components. Such granule fusion enables e.g., myeloperoxidase-processing of intragranular oxygen radicals, a key event in the formation of neutrophil extracellular traps (Björnsdottir et al., 2015)[1]. Described herein are data that show the quantification of such heterotypic granule-granule fusion by the use of imaging flow cytometry, a technique that combines flow cytometry with microscopy. The analysis described is based on immunofluorescent staining of established granule markers (lactoferrin and/or NGAL for one granule subset; the specific granules, and CD63 for another granule subset, the azurophil granules) and calculation of a colocalization score for resting and PMA-stimulated neutrophils.
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| 2. |
- Bru, Thomas, et al.
(författare)
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Characterisation of the mechanical and fracture properties of a uni-weave carbon fibre/epoxy non-crimp fabric composite
- 2016
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Ingår i: Data in Brief. - : Elsevier BV. - 2352-3409. ; 6, s. 680-695
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Tidskriftsartikel (refereegranskat)abstract
- A complete database of the mechanical properties of an epoxy polymer reinforced with uni-weave carbon fibre non-crimp fabric (NCF) is established. In-plane and through-the-thickness tests were performed on unidirectional laminates under normal loading and shear loading. The response under cyclic shear loading was also measured. The material has been characterised in terms of stiffness, strength, and failure features for the different loading cases. The critical energy release rates associated with different failure modes in the material were measured from interlaminar and translaminar fracture toughness tests. The stress–strain data of the tensile, compressive, and shear test specimens are included. The load–deflection data for all fracture toughness tests are also included. The database can be used in the development and validation of analytical and numerical models of fibre reinforced plastics (FRPs), in particular FRPs with NCF reinforcements.
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| 3. |
- Cai, Demin, et al.
(författare)
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Expression of hepatic miRNAs targeting porcine glucocorticoid receptor (GR) 3'UTR in the neonatal piglets under a maternal gestational betaine supplementation
- 2016
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Ingår i: Data in Brief. - : Elsevier BV. - 2352-3409. ; 6, s. 4-7
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Tidskriftsartikel (refereegranskat)abstract
- Glucocorticoid receptor (GR) has been previously demonstrated an important transcriptional factor of hepatic metabolic genes in the neonates under a maternal gestational betaine supplementation ("Gestational dietary betaine supplementation suppresses hepatic expression of lipogenic genes in neonatal piglets through epigenetic and glucocorticoid receptor-dependent mechanisms" Cai et al., 2015 [1]). Here we provide accompanying data about the expression of hepatic miRNAs targeting porcine GR 3'UTR in the neonatal piglets. Liver samples were obtained and RNA was isolated. RNA was polyadenylated by poly (A) polymerase and then dissolved and reverse transcribed using poly (T) adapter. The diluted cDNA were used in each real-time PCR assay. The sequences of all the porcine miRNAs were acquired from miRBase (http://www.mirbase.org/). miRNAs targeting GR were predicted using the PITA algorithm. Among all the predicted miRNAs, 4 miRNAs targeting GR were quantitated by real-time PCR and miRNA-124a, which has been identified to target GR 3'UTR [2], [3], was more highly expressed in betaine-exposed neonatal livers.
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| 4. |
- Chen, Moashan, et al.
(författare)
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Data on the expression of cellular lncRNAs in human adenovirus infected cells
- 2016
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Ingår i: Data in Brief. - Elsevier : Elsevier BV. - 2352-3409. ; 8, s. 1263-1279
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Tidskriftsartikel (refereegranskat)abstract
- Expression of cellular long non-coding RNAs (lncRNAs) in human primary lung fibroblasts (IMR-90) during the course of adenovirus type 2 (Ad2) infection was studied by strand-specific whole transcriptome sequencing. In total, 645 cellular lncRNAs were expressed at a significant level and 398 of them were changed more than 2-fold. The changes in expression followed a distinct temporal pattern. Significantly, 80% of the changes occurred at the late phase and 80% of the de-regulated lncRNAs were up-regulated. The three largest groups of deregulated lncRNAs were 125 antisense RNAs, 111 pseudogenes and 85 long intergenic non-coding RNAs (lincRNAs). Lastly, more than 36% of lncRNAs have been shown to interact with RNA binding proteins.
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| 5. |
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| 6. |
- Garcia, Danilo, 1973, et al.
(författare)
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Time Perspective and Well-Being: Swedish Survey Questionnaires and Data
- 2016
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Ingår i: Data in Brief. - : Elsevier BV. - 2352-3409. ; 9, s. 183-193
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Tidskriftsartikel (refereegranskat)abstract
- The data pertains 448 Swedes’ responses to questionnaires on time perspective (Zimbardo Time Perspective Inventory), temporal life satisfaction (Temporal Satisfaction with Life Scale), affect (Positive Affect and Negative Affect Schedule), and psychological well-being (Ryff’s Scales of Psychological Well-Being—short version). The data was collected among university students and individuals at a training facility (see [1]). Since there were no differences in any of the other background variables, but exercise frequency, all subsequent analyses were conducted on the 448 participants as one single sample. In this article we include the Swedish versions of the questionnaires used to operationalize the time perspective and well-being variables. The data is available, SPSS file, as supplementary material in this article. We used the Expectation-Maximization Algorithm to input missing values. Little’s Chi-Square test for Missing Completely at Random showed a χ2 = 67.25 (df = 53, p = .09) for men and χ2 = 77.65 (df = 72, p = .31) for women. These values suggested that the Expectation-Maximization Algorithm was suitable to use on this data for missing data imputation.
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| 7. |
- Gustafsson, Malin, et al.
(författare)
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Tree traits and canopy closure data from an experiment with 34 planted species native to Sabah, Borneo
- 2016
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Ingår i: Data in Brief. - : Elsevier BV. - 2352-3409. ; 6, s. 466-470
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Tidskriftsartikel (refereegranskat)abstract
- The data presented in this paper is supporting the research article "Life history traits predict the response to increased light among 33 tropical rainforest tree species" [3]. We show basic growth and survival data collected over the 6 years duration of the experiment, as well as data from traits inventories covering 12 tree traits collected prior to and after a canopy reduction treatment in 2013. Further, we also include canopy closure and forest light environment data from measurements with hemispherical photographs before and after the treatment. (C) 2016 The Authors. Published by Elsevier Inc.
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| 8. |
- Holdfeldt, André, et al.
(författare)
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Data on human neutrophil activation induced by pepducins with amino acid sequences derived from β2AR and CXCR4.
- 2016
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Ingår i: Data in brief. - : Elsevier BV. - 2352-3409. ; 8, s. 411-4
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Tidskriftsartikel (refereegranskat)abstract
- The data described here is related to the research article titled (Gabl et al., 2016) [1]. Pepducins with peptide sequence derived from one of the intracellular domains of a given G-protein coupled receptor (GPCR) can either activate or inhibit cell functions. Here we include data on human neutrophil function induced by pepducins derived from β2AR (ICL3-8) and CXCR4 (ATI-2341), respectively. ICL3-8 exerts neither direct activating effect on the NADPH-oxidase as measured by superoxide release nor inhibitory effect on FPR signaling. ATI-2341 dose-dependently triggers neutrophil activation and these cells were subsequently desensitized in their response to FPR2 specific agonists F2Pal10 and WKYMVM. Moreover, the ATI-2341 response is inhibited by PBP10 and the peptidomimetic Pam-(Lys-betaNSpe)6-NH2 (both are FPR2 specific inhibitors), but not to the FPR1 specific inhibitor cyclosporine H.
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| 9. |
- Hörnaeus, Katarina, et al.
(författare)
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Mass spectrometry data from a quantitative analysis of protein expression in gills of immuno-challenged blue mussels (Mytilus edulis)
- 2016
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Ingår i: Data in Brief. - Elsevier : Elsevier BV. - 2352-3409. ; 8, s. 470-473
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Tidskriftsartikel (refereegranskat)abstract
- Here, we provide the dataset associated with our research article on the potential effects of ocean acidification on antimicrobial peptide (AMP) activity in the gills of Mytilus edulis, "Impact of ocean acidification on antimicrobial activity in gills of the blue mussel (Mytilus edulis)" [1]. Blue mussels were stimulated with lipopolysaccharides and samples were collected at different time points post injection. Protein extracts were prepared from the gills, digested using trypsin and a full in-depth proteome investigation was performed using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Protein identification and quantification was performed using the MaxQuant 1.5.1.2 software, "MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification" [2].
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| 10. |
- Mereiter, Stefan, et al.
(författare)
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Glycomic and sialoproteomic data of gastric carcinoma cells overexpressing ST3GAL4.
- 2016
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Ingår i: Data in brief. - : Elsevier BV. - 2352-3409. ; 7, s. 814-33
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Tidskriftsartikel (refereegranskat)abstract
- Gastric carcinoma MKN45 cells stably transfected with the full-length ST3GAL4 gene were characterised by glycomic and sialoproteomic analysis. Complementary strategies were applied to assess the glycomic alterations induced by ST3GAL4 overexpression. The N- and O-glycome data were generated in two parallel structural analyzes, based on PGC-ESI-MS/MS. Data on glycan structure identification and relative abundance in ST3GAL4 overexpressing cells and respective mock control are presented. The sialoproteomic analysis based on titanium-dioxide enrichment of sialopeptides with subsequent LC-MS/MS identification was performed. This analysis identified 47 proteins with significantly increased sialylation. The data in this article is associated with the research article published in Biochim Biophys Acta "Glycomic analysis of gastric carcinoma cells discloses glycans as modulators of RON receptor tyrosine kinase activation in cancer" [1].
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| 11. |
- Roy, Amit, et al.
(författare)
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Data set for diet specific differential gene expression analysis in three Spodoptera moths
- 2016
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Ingår i: Data in Brief. - : Elsevier BV. - 2352-3409. ; 8, s. 448-455
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Tidskriftsartikel (refereegranskat)abstract
- Examination of closely related species pairs is suggested for evolutionary comparisons of different degrees of polyphagy, which we did here with three taxa of lepidopteran herbivores,Spodopteraspp (S. littoralis,S. frugiperdamaize (C) and rice (R) strains) for a RNAseq analysis of the midguts from the 3rd instar insect larvae for differential metabolic responses after feeding on pinto bean based artificial diet vs maize leaves. Paired-end (2×100bp) Illumina HiSeq2500 sequencing resulted in a total of 24, 23, 24, and 21 million reads for the SF-C-Maize, SF-C-Pinto, SF-R-Maize, SF-R Pinto, and a total of 35 and 36 million reads for the SL-Maize and SL-Pinto samples, respectively. After quality control measures, a total of 62.2 million reads from SL and 71.7 million reads from SF were used for transcriptome assembly (TA). The resulting finalde novoreference TA (backbone) for the SF taxa contained 37,985 contigs with a N50 contig size of 1030bp and a maximum contig length of 17,093bp, while for SL, 28,329 contigs were generated with a N50 contig size of 1980bp and a maximum contig length of 18,267bp. The data presented herein contains supporting information related to our research article Roy et al. (2016)http://dx.doi.org/10.1016/j.ibmb.2016.02.006
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| 12. |
- Shao, Linus Ruijin, 1964, et al.
(författare)
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Quantitative analysis of hormones and inflammatory cytokines in Chlamydia trachomatis-infected women with tubal ectopic pregnancy and early intrauterine pregnancy
- 2016
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Ingår i: Data in Brief. - : Elsevier BV. - 2352-3409. ; 6, s. 135-142
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Tidskriftsartikel (refereegranskat)abstract
- © 2015 The Authors. In this data, non-pregnant women during the menstrual cycle, women with normal intrauterine pregnancy (IUP), and women with tubal ectopic pregnancy (EP) after informed consent were included. The serum levels of 17β-estradiol, progesterone, testosterone, beta-human chorionic gonadotropin, interleukin (IL)-1β, IL-4, IL-6, IL-7, IL-8, IL-10, tumor necrosis factor α (TNFα), and interferon-γ (IFN-γ), epidermal growth factor, the Chlamydia (C.) trachomatis IgG and HSP60 were analyzed. Receiver operating characteristic analysis was used to assess the diagnostic discrimination of tubal EP and gestational age-matched IUP. Our data show that C. trachomatis infection is associated with IL-8 levels, which had excellent discriminative validity in positively identifying tubal EP (concomitant with C. trachomatis infection) from IUP and non-pregnant conditions regardless of C. trachomatis infection.
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| 13. |
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| 14. |
- Zhao, Ying, et al.
(författare)
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The effect of statins therapy in aortic stenosis : Meta-analysis comparison data of RCTs and observationals.
- 2016
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Ingår i: Data in brief. - : Elsevier BV. - 2352-3409. ; 7, s. 357-361
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Tidskriftsartikel (refereegranskat)abstract
- Aortic stenosis has been shown to share the same risk factors as atherosclerosis which suggested a potential benefit from statins therapy. Fourteen studies which provided the effect of statins treatment on aortic stenosis (AS) were meta-analyzed, including 5 randomized controlled trials (RCTs) and 9 observational studies. In the RCTs, statins did not have any influence on peak aortic valve velocity, peak valve gradient, mean valve gradient, aortic valve area and aortic calcification compared to controls. In the observational studies, the peak valve velocity, peak gradient and aortic valve area showed less progression in the statins group compared to controls. This article describes data related article title "The effect of statins on valve function and calcification in aortic stenosis: a meta-analysis" (Zhao et al., 2016) [1].
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