Sökning: WFRF:(Blagova Olga)
> (2019) >
Crystal structure a...
Crystal structure and substrate interactions of an unusual fungal non-CBM carrying GH26 endo-β-mannanase from Yunnania penicillata
-
- von Freiesleben, Pernille (författare)
- Technical University of Denmark,Novozymes A/S
-
- Moroz, Olga V. (författare)
- University of York
-
- Blagova, Elena (författare)
- University of York
-
visa fler...
-
- Wiemann, Mathias (författare)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
-
- Spodsberg, Nikolaj (författare)
- Novozymes A/S
-
- Agger, Jane W. (författare)
- Technical University of Denmark
-
- Davies, Gideon J. (författare)
- University of York
-
- Wilson, Keith S. (författare)
- University of York
-
- Stålbrand, Henrik (författare)
- Lund University,Lunds universitet,Biokemi och Strukturbiologi,Centrum för Molekylär Proteinvetenskap,Kemiska institutionen,Institutioner vid LTH,Lunds Tekniska Högskola,Biochemistry and Structural Biology,Center for Molecular Protein Science,Department of Chemistry,Departments at LTH,Faculty of Engineering, LTH
-
- Meyer, Anne S. (författare)
- Technical University of Denmark
-
- Krogh, Kristian B.R.M. (författare)
- Novozymes A/S
-
visa färre...
-
(creator_code:org_t)
- 2019-02-19
- 2019
- Engelska.
-
Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 9:1
- Relaterad länk:
-
http://dx.doi.org/10... (free)
-
visa fler...
-
https://www.nature.c...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Endo-β(1 → 4)-mannanases (endomannanases) catalyse degradation of β-mannans, an abundant class of plant polysaccharides. This study investigates structural features and substrate binding of YpenMan26A, a non-CBM carrying endomannanase from Yunnania penicillata. Structural and sequence comparisons to other fungal family GH26 endomannanases showed high sequence similarities and conserved binding residues, indicating that fungal GH26 endomannanases accommodate galactopyranosyl units in the −3 and −2 subsites. Two striking amino acid differences in the active site were found when the YpenMan26A structure was compared to a homology model of Wsp.Man26A from Westerdykella sp. and the sequences of nine other fungal GH26 endomannanases. Two YpenMan26A mutants, W110H and D37T, inspired by differences observed in Wsp.Man26A, produced a shift in how mannopentaose bound across the active site cleft and a decreased affinity for galactose in the −2 subsite, respectively, compared to YpenMan26A. YpenMan26A was moreover found to have a flexible surface loop in the position where PansMan26A from Podospora anserina has an α-helix (α9) which interacts with its family 35 CBM. Sequence alignment inferred that the core structure of fungal GH26 endomannanases differ depending on the natural presence of this type of CBM. These new findings have implications for selecting and optimising these enzymes for galactomannandegradation.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
von Freiesleben, ...
-
Moroz, Olga V.
-
Blagova, Elena
-
Wiemann, Mathias
-
Spodsberg, Nikol ...
-
Agger, Jane W.
-
visa fler...
-
Davies, Gideon J ...
-
Wilson, Keith S.
-
Stålbrand, Henri ...
-
Meyer, Anne S.
-
Krogh, Kristian ...
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Biokemi och mole ...
- Artiklar i publikationen
-
Scientific Repor ...
- Av lärosätet
-
Lunds universitet