| 1. |
- Huber, Birgit, et al.
(författare)
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Ochrobactrum pituitosum sp. nov., isolated from an industrial environment.
- 2010
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Ingår i: International journal of systematic and evolutionary microbiology. - : Microbiology Society. - 1466-5026 .- 1466-5034. ; 60:Pt 2, s. 321-6
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Tidskriftsartikel (refereegranskat)abstract
- Strain CCUG 50899, a Gram-negative, rod-shaped, non-spore-forming, motile bacterium isolated from industrial environment in Sweden and tentatively assigned to the species Ochrobactrum anthropi, was studied in order to clarify its taxonomic status. 16S rRNA gene sequence similarities placed the strain in the genus Ochrobactrum, sharing highest similarity with the type strains of Ochrobactrum rhizosphaerae (99.3 %), Ochrobactrum thiophenivorans (98.7 %), Ochrobactrum pseudogrignonense (98.6 %) and Ochrobactrum grignonense (98.5 %). The fatty acid profile of [O. anthropi] CCUG 50899 (major fatty acids C(18 : 1)omega7c and C(19 : 0) cyclo omega8c and presence of C(18 : 1) 2-OH), the polar lipid profile (diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, two unknown aminolipids and an unknown phospholipid), the presence of the quinone system ubiquinone Q-10 and a polyamine pattern with the major compounds putrescine and spermidine and moderate amounts of sym-homospermidine supported its affiliation to the genus Ochrobactrum. DNA-DNA reassociation experiments with the type strains of its closest relatives O. rhizosphaerae, O. pseudogrignonense, O. thiophenivorans and O. grignonense demonstrated that [O. anthropi] CCUG 50899 should be placed in a novel species, which is distinguishable from related species by a set of biochemical traits. Based on these data, reclassification of [O. anthropi] CCUG 50899 as the type strain of a novel species appears to be justified. Hence, we describe a novel species to accommodate this strain, for which we propose the name Ochrobactrum pituitosum sp. nov. The type strain is CCUG 50899(T) (=DSM 22207(T)).
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| 2. |
- Kämpfer, Peter, et al.
(författare)
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A revision of Massilia La Scola et al. 2000, with an emended description of the genus, and the inclusion of all species of the genus Naxibacter as new combinations and a proposal of Massilia consociata sp. nov.
- 2011
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Ingår i: International journal of systematic and evolutionary microbiology. - : Microbiology Society. - 1466-5034 .- 1466-5026. ; 61:7, s. 1528-1533
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Tidskriftsartikel (refereegranskat)abstract
- A Gram-negative, rod-shaped, non-spore-forming bacterium originating from a human clinical specimen was studied for its taxonomic position. 16S rRNA gene sequence similarity studies clearly allocated this strain (CCUG 58010T) to the Betaproteobacteria, closely related to members of both genera Massilia and Naxibacter. Naxibacter varians was shown to be the most closely related species on the basis of 16S rRNA gene sequence similarity (97,5%), followed by Massilia niastensis (96.8%) and Massilia aerilata (96.4%). Similarities to all other Naxibacter and Massilia species were in a range of 93.9 - 96.2%. Chemotaxonomic data (major ubiquinone: Q-8; major polar lipids: phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol, and major fatty acids: summed feature 3 (C16:1omega7c/2-OH and/or iso-C15:0 2-OH), C16:0, C18:1omega7c, C12:0 and with C10:0 3-OH as hydroxylated fatty acid) supported the affiliation of the isolate to these genera, which are sharing these chemotaxonomic traits. DNA-DNA hybridization of strain CCUG 58010T with the type strain of N. varians CCUG 35299T resulted in values of 39.2% (reciprocal, 50%) and physiological and biochemical tests allowed also a phenotypic differentiation of the isolate from the most closely related species. There is currently no justification for a division of Massilia and Naxibacter and for this reason a proposal is made to transfer all Naxibacter species to the genus Massilia, as Massilia alkalitolerans comb. nov., Massilia varians comb. nov., Massilia haematophila comb. nov, and Massilia suwonensis comb. nov. Strain CCUG 58010T represents a novel species, for which the name Massilia consociata sp. nov. is proposed, with the type strain CCUG 58010T (= CCM 7792T).
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| 3. |
- Kämpfer, Peter, et al.
(författare)
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Hydrotalea flava gen. nov., sp. nov., a new species of the phylum Bacteroidetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas and Hydrotalea to the family Chitinophagaceae fam. nov.
- 2011
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Ingår i: International journal of systematic and evolutionary microbiology. - : Microbiology Society. - 1466-5034 .- 1466-5026. ; 61:3, s. 518-523
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Tidskriftsartikel (refereegranskat)abstract
- Three orange pigmented bacterial isolates (strain CCUG 51397T, CCUG 53736 and CCUG 53920) isolated from water of different locations in Southern Sweden were studied for their taxonomic position in a polyphasic approach. Comparative analysis of 16S rRNA gene sequences showed that these bacteria had <93% similarity to all described species of the genera Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas, and Chitinophaga. The three organisms grouped most closely to Sediminibacterium salmoneum, but showed only 92.5% similarity to the only species of this genus. The fatty acid profile showed high amounts of iso 15:0, iso 17:0 3-OH and iso 15:1G and minor amounts of iso 15:0 3-OH, iso 16:0 3-OH and other fatty acids, allowing the differentiation of the genus from the other genera. Biochemical test patterns differed also from that of Sediminibacterium salmoneum and the other genera. On the basis of these data, it is proposed that the strains are representative of a novel genus, for which the name Hydrotalea gen. nov. is proposed. The type strain for Hydrotalea flava sp. nov. is CCUG 51397T = CCM 7760T. A formal allocation of the genera Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas, and Chitinophaga to the family Chitinophagaceae fam. nov. is proposed .
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| 4. |
- Kämpfer, Peter, et al.
(författare)
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Massilia oculi sp. nov., isolated from a human clinical specimen.
- 2012
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Ingår i: International journal of systematic and evolutionary microbiology. - : Microbiology Society. - 1466-5034 .- 1466-5026. ; 62:Pt 2, s. 364-9
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Tidskriftsartikel (refereegranskat)abstract
- A gram-negative, rod-shaped, non-spore-forming bacterium (strain CCUG 43427A(T)) was isolated from a patient suffering from endophthalmitis and its taxonomic position was studied. 16S rRNA gene sequence analysis indicated that this strain was a member of the genus Massilia. Strain CCUG 43427A(T) was most closely related to the type strains of Massilia timonae (97.4% 16S rRNA gene sequence similarity) and Massilia aurea (97.2%); levels of similarity to the type strains of all other recognized Massilia species were below 97.0%. Chemotaxonomic data [Q-8 as major ubiquinone; phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as major polar lipids; and summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH), C(16:0), C(18:1)ω7c, C(12:0) and C(10:0) 3-OH as major fatty acids] supported the affiliation of the isolate to the genus Massilia. Levels of DNA-DNA relatedness of strain CCUG 43427A(T) with M. timonae CCUG 45783(T) and M. aurea AP13(T) were 60.6% (reciprocal, 55.8%) and 58.1% (reciprocal, 34.0%), respectively. Strain CCUG 43427A(T) could be differentiated from its closest phylogenetic neighbours based on a range of phenotypic characteristics. Strain CCUG 43427A(T) is therefore considered to represent a novel species of the genus Massilia, for which the name Massilia oculi sp. nov. is proposed. The type strain is CCUG 43427A(T) (=CCM 7900(T)).
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| 5. |
- Scheiben, D., et al.
(författare)
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Observations of middle atmospheric H2O and O-3 during the 2010 major sudden stratospheric warming by a network of microwave radiometers
- 2012
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Ingår i: Atmospheric Chemistry and Physics. - : Copernicus GmbH. - 1680-7316 .- 1680-7324. ; 12:16, s. 7753-7765
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Tidskriftsartikel (refereegranskat)abstract
- In this study, we present middle atmospheric water vapor (H2O) and ozone (O-3) measurements obtained by ground-based microwave radiometers at three European locations in Bern (47 degrees N), Onsala (57 degrees N) and Sodankyla (67 degrees N) during Northern winter 2009/2010. In January 2010, a major sudden stratospheric warming (SSW) occurred in the Northern Hemisphere whose signatures are evident in the ground-based observations of H2O and O-3. The observed anomalies in H2O and O-3 are mostly explained by the relative location of the polar vortex with respect to the measurement locations. The SSW started on 26 January 2010 and was most pronounced by the end of January. The zonal mean temperature in the middle stratosphere (10 hPa) increased by approximately 25 Kelvin within a few days. The stratospheric vortex weakened during the SSW and shifted towards Europe. In the mesosphere, the vortex broke down, which lead to large scale mixing of polar and midlatitudinal air. After the warming, the polar vortex in the stratosphere split into two weaker vortices and in the mesosphere, a new, pole-centered vortex formed with maximum wind speed of 70 ms(-1) at approximately 40 degrees N. The shift of the stratospheric vortex towards Europe was observed in Bern as an increase in stratospheric H2O and a decrease in O-3. The breakdown of the mesospheric vortex during the SSW was observed at Onsala and Sodankyla as a sudden increase in mesospheric H2O. The following large-scale descent inside the newly formed mesospheric vortex was well captured by the H2O observations in Sodankyla. In order to combine the H2O observations from the three different locations, we applied the trajectory mapping technique on our H2O observations to derive synoptic scale maps of the H2O distribution. Based on our observations and the 3-D wind field, this method allows determining the approximate development of the stratospheric and mesospheric polar vortex and demonstrates the potential of a network of ground-based instruments.
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