Sökning: WFRF:(Kleywegt Gerard J.)
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Glucomannan and bet...
Glucomannan and beta-glucan degradation by Mytilus edulis Cel45A : Crystal structure and activity comparison with GH45 subfamily A, B and C
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- Okmane, Laura (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Swedish Univ Agr Sci, Dept Mol Sci, POB 7015, SE-75007 Uppsala, Sweden.
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- Nestor, Gustav (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Swedish Univ Agr Sci, Dept Mol Sci, POB 7015, SE-75007 Uppsala, Sweden.
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- Jakobsson, Emma (författare)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,Basque Res & Technol Alliance BRTA, CIC BiomaGUNE, San Sebastian 20014, Guipuzcoa, Spain.
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- Xu, Bingze (författare)
- Uppsala universitet,Centrum för ytbioteknik,Karolinska Inst, Dept Med Biochem & Biophys, Med Inflammat Res, SE-17177 Solna, Sweden.
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- Igarashi, Kiyohiko (författare)
- Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biomat Sci, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan.
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- Sandgren, Mats (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Swedish Univ Agr Sci, Dept Mol Sci, POB 7015, SE-75007 Uppsala, Sweden.
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- Kleywegt, Gerard J. (författare)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi,EMBL EBI, Wellcome Genome Campus, Cambridge CB10 1SD, England.
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- Ståhlberg, Jerry (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för Molekylära vetenskaper,Department of Molecular Sciences,Swedish Univ Agr Sci, Dept Mol Sci, POB 7015, SE-75007 Uppsala, Sweden.
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(creator_code:org_t)
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- Elsevier, 2022
- 2022
- Engelska.
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Ingår i: Carbohydrate Polymers. - : Elsevier. - 0144-8617 .- 1879-1344. ; 277
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https://doi.org/10.1...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The enzymatic hydrolysis of barley beta-glucan, konjac glucomannan and carboxymethyl cellulose by a beta-1,4-D-endoglucanase MeCel45A from blue mussel, Mytilus edulis, which belongs to subfamily B of glycoside hydrolase family 45 (GH45), was compared with GH45 members of subfamilies A (Humicola insolens HiCel45A), B (Trichoderma reesei TrCel45A) and C (Phanerochaete chrysosporium PcCel45A). Furthermore, the crystal structure of MeCel45A is reported.Initial rates and hydrolysis yields were determined by reducing sugar assays and product formation was characterized using NMR spectroscopy. The subfamily B and C enzymes exhibited mannanase activity, whereas the subfamily A member was uniquely able to produce monomeric glucose. All enzymes were confirmed to be inverting glycoside hydrolases. MeCel45A appears to be cold adapted by evolution, as it maintained 70% activity on cellohexaose at 4 degrees C relative to 30 degrees C, compared to 35% for TrCel45A. Both enzymes produced cellobiose and cellotetraose from cellohexaose, but TrCel45A additionally produced cellotriose.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- Endoglucanase
- Blue mussel
- Cel45A
- GH45
- Beta-glucan
- Glucomannan
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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