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| 3. |
- Ohlson, Sten, et al.
(författare)
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High Performance Liquid Affinity Chromatography: a New Tool in Biotechnology
- 1989
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Ingår i: Trends in Biotechnology. - : Elsevier BV. - 0167-7799 .- 1879-3096. ; 7:7, s. 179-186
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Tidskriftsartikel (refereegranskat)abstract
- In high performance liquid affinity chromatography (HPLAC), the biospecificity of affinity chromatography is combined with the robust support materials of HPLC. HPLAC can provide specificity, speed and high resolution in chromatography both on analytical and preparative scales. The number of commercially available HPLAC matrices is an indicator of the growing use of the technique.
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| 6. |
- Belew, M, et al.
(författare)
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Structure-activity studies on synthetic analogs to vasoactive peptides derived from human fibrinogen.
- 1980
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Ingår i: Biochimica et Biophysica Acta. - 0006-3002 .- 1878-2434. ; 632:1, s. 87-94
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Tidskriftsartikel (refereegranskat)abstract
- Counterparts to two vasoactive peptides previously isolated from fibrin(ogen) degraded by plasmin (EC 3.4.21.7) were synthesized by the solid phase procedure. The synthetic undecapeptide (Ser-Gln-Leu-Gln-Lys-Val-Pro-Pro-Glu-Trp-Lys) was isolated in a homogeneous state by chromatography on Sephadex G-25 and DEAE-Sepharose CL-6B and the pentapeptide (Ala-Arg-Pro-Ala-Lys) by chromatography on BioGel P-6 and column zone electrophoresis. The effect of these two peptides and of fifteen analogs to the pentapeptide on microvascular permeability in rat skin was investigated. The two synthetic counterparts were as potent as the natural peptides. With respect to the analogs, the influence of different functional groups was first studied. This was followed by attempts to minimize the active structure, induce or relieve rigidity of the peptide back-bone or otherwise accomplish modifications by a change in chirality at critical positions. Our results show that the tetrapeptide Arg-Pro-Ala-Lys has the same effect on microvascular permeability as the pentapeptide in the assay system used. Basic amino acids at both ends, as well as a proline residue adjacent to the N-terminal amino acid appear important for full or essentially full activity. On the other hand, substitution of the Ala at position 4 with several other amino acids did not result in a significant loss in biological potency.
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| 7. |
- Bjornstad, T., et al.
(författare)
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Study of the Giant Gamow-Teller Resonance in Nuclear Beta-Decay - the Case of Ar-32
- 1985
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Ingår i: Nuclear Physics A. - : Elsevier BV. - 0375-9474. ; 443:2, s. 283-301
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Tidskriftsartikel (refereegranskat)abstract
- Delayed proton and gamma emissions following the β-decay of 32Ar have been studied. The half-life of 32Ar is 98 ± 2 ms, and the T = 2 analogue state in 32Cl lies at the excitation energy 5036 ± 12 keV. From the intensity of the feeding to this state the proton intensities can be converted to an absolute scale leading to a total proton branching ratio of (43 ± 3)%. From proton-gamma coincidence measurements it emerges that (1.9 ± 0.2)% of the protons lead to the first-excited state in 31S. A level scheme up to 8.75 MeV excitation in 32Cl has been constructed and the Gamow-Teller strength function has been deduced. The result is a renormalization of the axial-vector strength to (49 ± 5)% of the free-nucleon value.
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| 10. |
- Glad, M, et al.
(författare)
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Separation Agent
- 1983
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Patent (populärvet., debatt m.m.)
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| 11. |
- Göransson, L., et al.
(författare)
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Effects of different handling systems on pig feed quality
- 1982
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Ingår i: Animal Feed Science and Technology. - : Elsevier BV. - 0377-8401 .- 1873-2216. ; 7:1, s. 11-26
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Tidskriftsartikel (refereegranskat)abstract
- Separation of feed mixtures and its effect on the production traits of growing-finishing pigs was studied in three experiments. In Experiments I and II the effects of different types of equipment to prevent separation in a silo were studied. Experiment III dealt with separation along a feed transport chain. Experiments I and II clearly demonstrated an increase in feed quality variation by filling a silo without any arrangement to reduce separation. The last feed to be extracted from a silo filled in this manner showed an increasing amount of small particles and contents of crude fibre and ash, a decrease in the bulk density and also in the digestibility of nutrients and in the performance of the pigs. Filling a silo equipped with chutes by an auger or pneumatically through a cyclone did not create separation problems, and consequently there were no differences between these treatments in the production experiments. Separation measured by particle size, bulk density or chemical composition was not noticeable along the feed transport chain (Experiment III). There was no effect on performance of the pigs of their location along the chain. © 1982.
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| 12. |
- Hirsch, Jan M, et al.
(författare)
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The reversibility of the snuff-induced lesion : an experimental study in the rat
- 1986
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Ingår i: Journal of Oral Pathology & Medicine. - : Wiley. - 0904-2512 .- 1600-0714. ; 15:10, s. 540-543
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Tidskriftsartikel (refereegranskat)abstract
- Snuff lesions were induced in 30 rats. Ten of the snuff-exposed rats were killed immediately after 13 months snuff exposure, as were the 10 control animals. Ten rats were killed 1 month and 10 rats 4 months after the snuff administration had ceased. The rats exposed to snuff for 13 months exhibited hyperplastic, hyperorthokeratotic epithelium with focal mild atypia, focal ulcerations and marked subepithelial fibrosis. These changes were markedly reduced or absent in rats exposed to snuff and killed after a snuff-free interval of 1 or 4 months. Similar differences between the test-groups were seen in the epithelium lining the gingival sulcus of the lower incisors. This area seems to be more sensitive to chemical exposure than the oral mucosa proper as more severe microscopical changes were seen here. Snuff exposure results in the development of a hyperplastic, reactive, reversible lesion of the oral mucosa, suggesting that snuff predominantly has promoting activity when administered for a relatively short interval of time.
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| 13. |
- Johansson, S L, et al.
(författare)
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Snuff-induced carcinogenesis : effect of snuff in rats initiated with 4-nitroquinoline N-oxide
- 1989
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Ingår i: Cancer Research. - 0008-5472 .- 1538-7445. ; 49:11, s. 3063-3069
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Tidskriftsartikel (refereegranskat)abstract
- A canal in the lower lip to function as a reservoir for snuff was surgically created in 150 male Sprague-Dawley rats. The animals were randomized into five groups of 30 each: Group I received snuff twice a day, 5 days a wk; Group II was painted with propylene glycol (solvent control) on the hard palate 3 times a wk during 4 wk; Group III underwent painting on the hard palate with 4-nitroquinoline N-oxide (4-NQO) dissolved in propylene glycol, 3 times a wk for 4 wk; Group IV received 4-NQO as in Group III followed by snuff application as in Group I; and Group V received a cotton pellet dipped in saline twice a day, 5 days a wk. Treatment continued for up to 108 wk. There was no significant difference in mean survival time between the groups. Squamous cell tumors of the lip, oral and nasal cavities, esophagus, and forestomach were seen only in Groups I, III, and IV. Nine tumors of these organs were found in Group I (six carcinomas and three papillomas), nine in Group III (seven carcinomas and two papillomas), and ten in Group IV (eight carcinomas and two papillomas). The difference between each of these groups and the control groups (II and V) with regard to tumor incidence is statistically significant (P less than 0.05). In Group I, four oral cavity or lip carcinomas were found in 29 rats, a significant difference in relation to control rats (P less than 0.05). In addition, hyperplastic lesions of the lip, palate, and forestomach were significantly more common in Groups I and IV compared with Groups II, III, and V. The study has shown that snuff and 4-NQO by themselves have the potential to induce malignant tumors. Initiation with 4-NQO followed by snuff did not significantly enhance tumor formation.
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| 18. |
- Larsson, C.G., et al.
(författare)
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Diffraction of UV excited photoelectrons by an ordered overlayer
- 1983
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Ingår i: Physica Scripta. - 0031-8949 .- 1402-4896. ; T4, s. 44-46
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Tidskriftsartikel (refereegranskat)abstract
- Angle resolved UV photoemission spectra obtained from Cu(111)-(√3 × √3)R30° Xe and Cu(111)-(√3 × √3)R30° CO samples are compared with spectra obtained from the clean substrate. Interest is focussed on the adsorbate induced changes in the Cu3d band range of initial energies. From the similarity between the spectra observed for the ordered Xe and CO overlayers we conclude that the changes are primarily due to diffraction of the photoexcited Cu3d electrons by the adsorbate. This is supported also by calculated photoelectron energy distributions.
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| 19. |
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| 20. |
- Larsson, C, et al.
(författare)
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Subjective memory after subarachnoid haemorrhage.
- 1988
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Ingår i: Practical aspects of memory: Current resarch and issues.. - Chichester : Wiley. - 0471912344 - 0471918679 ; , s. 71-76
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 23. |
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| 24. |
- Larsson, L, et al.
(författare)
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The measurement of cell growth in coal slurries
- 1989
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Ingår i: Biotechnology Techniques. - 0951-208X. ; 3:5, s. 295-298
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Tidskriftsartikel (refereegranskat)abstract
- Microbial growth in coal slurries can be detected if surfactant is present in the samples by analysing the protein concentration after disintegration of the cells. Without surfactant present in the samples, the proteins are readily adsorbed on the coal surfaces. A suitable surfactant is sodium dodecyl sulphate at 0.5 to 1% of the sample volume
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| 25. |
- Larsson, P A, et al.
(författare)
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Effects of acyclovir on herpes simplex virus type 1 infection in mice treated with 12-O-tetradecanoylphorbol 13-acetate
- 1989
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Ingår i: Journal of General Virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 70:7, s. 1773-1778
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Tidskriftsartikel (refereegranskat)abstract
- The purpose of this study was to determine whether infectious herpes simplex virus type 1 (HSV-1) has tumorigenic properties and, if so, whether inhibition of the cytolytic replicative cycle of the virus after infection enhances tumour development. Eighty mice were subjected to repeated inoculation of HSV-1 on their upper lips after scarification, and systemic administration of acyclovir (ACV). 12-O-tetradecanoylphorbol 13-acetate (TPA) was used as the tumour promoter. The tumour incidence was compared to control groups each of 40 mice that were either not treated with ACV, not treated with TPA, not infected with HSV or only scarified. In the virus-infected group treated with ACV and TPA, 25% of the animals developed tumours. In the HSV-infected group treated with TPA only, 25% of the animals also developed tumours. The uninfected animals which were not treated with TPA developed tumours to a significantly lesser degree. In conclusion, the combined effects of HSV-1 and TPA, with or without ACV treatment, resulted in a significant increase in the number of tumours in comparison to the control groups.
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