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Träfflista för sökning "WFRF:(Miller A. A.) srt2:(1990-1994)"

Sökning: WFRF:(Miller A. A.) > (1990-1994)

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  • Tannock, Gerald W., et al. (författare)
  • Molecular characterization of a plasmid-borne (pGT633) erythromycin resistance determinant (ermGT) from lactobacillus reuteri 100-63
  • 1994
  • Ingår i: Plasmid. - : Elsevier BV. - 0147-619X. ; 31:1, s. 60-71
  • Tidskriftsartikel (refereegranskat)abstract
    • Lactobacillus reuteri 100-63 contains a 9.8 kb plasmid (pGT633) encoding resistance to the macrolide-lincosamide-streptogramin (MLS) type B antibiotics. The restriction endonucleases AccI, BamHI, Bcl I, EcoRI, EcoRV, HhaI, HindII, Hpa I, KpnI, SalI, and SspI were employed to establish a physical map of pGT633. Next, genetic transfer experiments were conducted to establish the host range of pGT633. The results revealed that pGT633 replicated autonomously and encoded constitutive resistance to erthromycin in a variety of bacterial hosts, including Bacillus subtilis BD170, Streptococcus sanguis DL1, Staphylococcus aureus RN4220, and Enterococcus faecalis 19433, as well as several Lactobacillus spp. A 2.2 kb BamHI fragment of pGT633 containing the genetic determinant (ermGT) encoding resistance to erythromycin was cloned into Escherichia coli. Determination of the nucleotide sequence of ermGT revealed a methylase gene 731 bp in length that was highly related (ca. 81% nucleotide and ca. 78% amino acid identity) to the ermC gene from S. aureus plasmid pE194. The leader sequences of ermGT and ermC were identical except for a single base change at nt 51.
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  • Wood, Sidney A J, et al. (författare)
  • A radiographic analysis of constriction locations for vowels
  • 1991
  • Ingår i: Papers in speech communication. Speech production (Reprinted from Journal of Phonetics 7, 1979, p.25-43). - 0883189585 ; 7
  • Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
    • Vocal tract area functions estimated from 38 sets of X-rayed vowel articulations collected from the literature and from new X-ray motion films of English and Arabic speech reveal four constriction locations: along the hard palate, along the soft palate, in the upper pharynx and in the lower larynx. Each location is appropriate for a definable class of vowel qualities, confirming the quantal nature of at least this aspect of vowel articulation. The acoustical, physiological and phonological implications are discussed. In a given phonotactic environment the precision of the constricting tongue manoeuvre was good. The only truly language specific difference was a preference for either the midpalatal or prepalatal location for palatal constrictions. The tongue muscles are found to be admirably situated for creating constrictions at the four locations.
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