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Effect of Cryoprese...
Effect of Cryopreservation on Nitric Oxide Production by Stallion Spermatozoa
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- Ortega Ferrusola, C. (författare)
- University of Extremadura, Spain
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- Gonzalez Fernandez, L. (författare)
- University of Extremadura, Spain
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- Macias Garcia, B. (författare)
- University of Extremadura, Spain
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- Salazar-Sandoval, C. (författare)
- University of Extremadura, Spain
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- Morillo Rodriguez, A. (författare)
- University of Extremadura, Spain
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- Rodriguez Martinez, Heriberto (författare)
- Swedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,Swedish University of Agriculture Science, Sweden
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- A. Tapia, J. (författare)
- University of Extremadura, Spain
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- J. Pena, F. (författare)
- University of Extremadura, Spain
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(creator_code:org_t)
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- Society for the Study of Reproduction, 2009
- 2009
- Engelska.
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Ingår i: Biology of Reproduction. - : Society for the Study of Reproduction. - 0006-3363 .- 1529-7268. ; 81:6, s. 1106-1111
- Relaterad länk:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://res.slu.se/i...
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Abstract
Ämnesord
Stäng
- The ability of stallion spermatozoa to produce nitric oxide (NO) before (fresh) and after freezing and thawing (FT) was evaluated by means of flow cytometry after loading the sperm suspension with the probe, 4,5-diaminofluorescenin diacetate. The presence of NO synthase (NOS) was investigated by Western blotting using anti-NOS1, anti-NOS3, or anti-universal NOS antibodies (Abs). While NO was detected both in fresh and FT sperm suspensions, its production increased after cryopreservation only when egg yolk was removed from the extender. Anti-NOS1 Ab intensively labeled a single band with an apparent molecular mass of approximately 83 kDa. On the other hand, the Ab developed against the NOS3 showed a band of approximately 96 kDa in fresh and FT sperm lysates. NO production was positively correlated with sperm motility and velocity after thaw, suggesting an NO role for the functionality of cryopreserved stallion spermatozoa; but the production of NO is compromised in egg yolk-containing extenders.
Ämnesord
- LANTBRUKSVETENSKAPER -- Husdjursvetenskap (hsv//swe)
- AGRICULTURAL SCIENCES -- Animal and Dairy Sience (hsv//eng)
Nyckelord
- assisted reproductive technology; cryopreservation; flow cytometry; gamete biology; nitric oxide; nitric oxide synthase; sperm; stallion
- TECHNOLOGY
- TEKNIKVETENSKAP
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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