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- Hillier, Ladeana W, et al.
(författare)
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Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution
- 2004
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Ingår i: Nature. - 0028-0836 .- 1476-4687. ; 432:7018, s. 695-716
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Tidskriftsartikel (refereegranskat)abstract
- We present here a draft genome sequence of the red jungle fowl, Gallus gallus. Because the chicken is a modern descendant of the dinosaurs and the first non-mammalian amniote to have its genome sequenced, the draft sequence of its genome--composed of approximately one billion base pairs of sequence and an estimated 20,000-23,000 genes--provides a new perspective on vertebrate genome evolution, while also improving the annotation of mammalian genomes. For example, the evolutionary distance between chicken and human provides high specificity in detecting functional elements, both non-coding and coding. Notably, many conserved non-coding sequences are far from genes and cannot be assigned to defined functional classes. In coding regions the evolutionary dynamics of protein domains and orthologous groups illustrate processes that distinguish the lineages leading to birds and mammals. The distinctive properties of avian microchromosomes, together with the inferred patterns of conserved synteny, provide additional insights into vertebrate chromosome architecture.
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| 2. |
- Arbring, K, et al.
(författare)
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Cortisol response to heelstick stressor in preterm infants
- 2000
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Ingår i: Prenatal and Neonatal Medicine. - 1359-8635 .- 1473-0774. ; 5:3, s. 182-185
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Tidskriftsartikel (refereegranskat)abstract
- To evaluate the hormonal response to stress in healthy preterm infants, we measured concentrations of serum cortisol at baseline and after capillary heelstick. Eleven preterm infants, five girls and six boys, with gestational ages ranging from 30 to 34 weeks, were studied. We measured the serum cortisol concentration before and 30 min after capillary heelstick on days 1, 3 and 7 of life. On days 3 and 7, but not on day 1, the rise in cortisol was significant (p = 0.02 and 0.04, respectively). The reduced response on day 1 can probably be explained by the significantly higher baseline concentrations. We suggest that a test like this can be useful in evaluating the hormonal stress response in preterm as well as full-term infants.
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| 7. |
- Nelson, A E, et al.
(författare)
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Diagnosis of a patient with oncogenic osteomalacia using a phosphate uptake bioassay of serum and magnetic resonance imaging
- 2001
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Ingår i: European Journal of Endocrinology. - : Oxford University Press (OUP). - 0804-4643 .- 1479-683X. ; 145:4, s. 469-476
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Tidskriftsartikel (refereegranskat)abstract
- A previously healthy man with no family history of fractures presented with muscle pain, back pain and height loss. Investigations revealed hypophosphataemia, phosphaturia, undetectable serum 1,25-dihydroxyvitamin D and severe osteomalacia on bone biopsy, suggestive of a diagnosis of oncogenic osteomalacia. Thorough physical examination did not locate a tumour. Support for the diagnosis was obtained by detection of phosphate uptake inhibitory activity in a blinded sample of the patient's serum using a renal cell bioassay. On the basis of detection of this bioactivity, a total body magnetic resonance (MR) examination was performed. A small tumour was located in the right leg. Removal of the tumour resulted in the rapid reversal of symptoms and the abnormal biochemistry typical of oncogenic osteomalacia. Inhibitory activity was also demonstrated using the bioassay in serum from two other patients with confirmed or presumptive oncogenic osteomalacia, but not in serum from two patients with hypophosphataemia of other origin. This is the first case to be reported in which the diagnosis of oncogenic osteomalacia was assisted by demonstration of inhibitory activity of the patient's serum in a renal cell phosphate bioassay that provided an impetus for total body MR imaging.
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| 8. |
- Nelson, J H, et al.
(författare)
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A novel and rapid PCR-based method for genotyping human papillomaviruses in clinical samples.
- 2000
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Ingår i: Journal of Clinical Microbiology. - 0095-1137 .- 1098-660X. ; 38:2, s. 688-95
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Tidskriftsartikel (refereegranskat)abstract
- Many human papillomavirus (HPV) genotypes are associated with cervical carcinoma. We demonstrate the utility of an innovative technique for genotyping of HPV in cervical tissue samples. This method provides an accurate means of identification of the specific HPV genotypes present in clinical specimens. By using the MY09-MY11 and the GP5(+)-GP6(+) consensus primer pairs, HPV sequences were amplified by nested PCR from DNA isolated from cervical smear samples. This led to the production of an approximately 140-bp PCR product from the L1 (major capsid) gene of any of the HPVs present in the sample. PCR was performed with a deoxynucleoside triphosphate mixture which resulted in the incorporation of deoxyuridine into the amplified DNA product at positions where deoxythymidine would normally be incorporated at a frequency of about once or twice per strand. Following the PCR, the product was treated with an enzyme mix that contains uracil N-glycosylase (UNG) and endonuclease IV. UNG removes the uracil base from the nucleotide, and endonuclease IV cleaves the phosphodiester bond at this newly formed abasic site, producing fragments of various sizes. By having end labeled one of the amplification primers, a DNA ladder which is analogous to a "T-sequencing ladder" was produced upon electrophoresis of the products. By comparing this T-sequencing ladder to the known sequences of HPVs, the genotypes of unknown HPV isolates in samples were assigned. Data showing the utility of this technique for the rapid analysis of clinical samples are presented.
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| 9. |
- Silvegren, Ulla, et al.
(författare)
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Historiska museet
- 2002
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Ingår i: Mellan liv och död : arkiv och museer i Lund berättar. - 9188846253 ; , s. 101-116
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Bokkapitel (populärvet., debatt m.m.)abstract
- Presentation av Lunds Universitets Historiska Museum för utställningen "Mellan liv och död" på Lunds Konsthall 31 augusti - 27 oktober 2002. En bok med samma titel publicerades i samband med utställningen, där tolv arkiv och museer i Lund berättar om sina unika verksamheter i samband med en då pågående utredning vid Lunds universitet om fyra museers framtid : Antikmuseet, Botaniska museet, Historiska museet och Zoologiska museet.
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| 10. |
- Tjus, Tomas, 1954, et al.
(författare)
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Interaction patterns between children and their teachers when using a specific multimedia and communication strategy: observations from children with autism and mixed intellectual disabilities.
- 2001
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Ingår i: Autism : the international journal of research and practice. - : SAGE Publications. - 1362-3613 .- 1461-7005. ; 5:2, s. 175-87
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Tidskriftsartikel (refereegranskat)abstract
- This study reports on observed interaction patterns between 20 children with autism and mixed intellectual disabilities (mean chronological age = 11:4 years; language age = 4:7 years) and their nine teachers working with a specially developed multimedia program aiming to increase literacy skills. An increase in verbal expression was found over time for the total group. Children with autism also showed increased enjoyment and willingness to seek help from their teachers. Teachers for both diagnostic groups reduced their instructions on how to handle the computer during the program but the decrease was greater in the teachers for children with autism. When the total group of children was subdivided according to language age (high versus low), it appears that those with a low language age showed an increase in verbal expressiveness from start to end of training. Those with a high language age showed increased enjoyment. It is concluded that more detailed studies of the interaction patterns between teachers and children are needed, and these should be related to children's language level as well as to diagnostic group.
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| 11. |
- Tjus, Tomas, 1954, et al.
(författare)
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Reading acquisition by implementing a multimedia intervention strategy for fifty children with autism or other learning and communication disabilities
- 2004
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Ingår i: Journal of Cognitive and Behavioral Psychotherapies. ; 4:2, s. 203-221
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Tidskriftsartikel (refereegranskat)abstract
- This study reports on the effect of teaching fifty children using a specially developed multimedia program for computers combined with verbal and social interactions with the children's teachers. Five groups of children (mean chronological age = 9:6 years, mental age = 7:10; and language age = 5:7) participated, children who had or were at risk for learning and language difficulties; autism, dyslexia, hearing impairment, children with deficits in attention, motor control and perception, and cerebral palsy with mental retardation. The aim was to maximise the opportunities for exploring literacy and language structures through different modes. All children used the program in addition to their ordinary reading and language training. A quasi-experimental design that included measures of reading during baseline, treatment, and follow-up periods was used throughout the study. Most children significantly increased their reading gain from baseline to treatment but the clearest gain was noted for the group of children with autism. It is concluded that a strategy that combines a motivating multimedia program with focused interactions with the teacher might improve the reading development in children with various learning disabilities.
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