| 1. |
- Pfaller, M.A., et al.
(författare)
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Twelve years of fluconazole in clinical practice : Global-trends in species distribution and fluconazole susceptibility of bloodstream isolates of Candida
- 2004
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Ingår i: Clinical Microbiology and Infection. - : Elsevier BV. - 1198-743X .- 1469-0691. ; 10:SUPPL. 1, s. 11-23
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Tidskriftsartikel (refereegranskat)abstract
- We determined the species distribution and in-vitro susceptibility of 6082 bloodstream infection (BSI) isolates of Candida spp. collected from 250 medical centres in 32 nations over a 10-year period from 1992 through 2001. The species included 3401 C. albicans, 984 C. glabrata, 796 C. parapsilosis, 585 C. tropicalis, 153 C. krusei, 67 C. lusitaniae, 48 C. guilliermondii, 10 C. famata, 10 C. kefyr, six C. pelliculosa, five C. rugosa, four C. lipolytica, three C. dubliniensis, three C. inconspicua, two C. sake and one isolate each of C. lambica, C. norvegensis and C. zeylanoides. Minimum inhibitory concentration determinations were made using the National Committee for Clinical Laboratory Standards reference broth microdilution method. Variation in the rank order and frequency of the different species of Candida was observed over time and by geographic area. The proportion of BSI due to C. albicans and C. glabrata increased and C. parapsilosis decreased over time in Canada, the USA and Europe. C. glabrata was an infrequent cause of BSI in Latin America and the Asia-Pacific region. Very little variation in fluconazole susceptibility was observed among isolates of C. albicans, C. tropicalis and C. parapsilosis. These species accounted for 78% of all BSI and remained highly susceptible (91-100% susceptible) to fluconazole from 1992 to 2001 irrespective of geographic origin. The prevalence of fluconazole resistance among C. glabrata isolates was variable both over time and among the various countries and regions. Resistance to fluconazole among C. glabrata isolates was greatest in the USA and varied by US census region (range 0-23%). These observations are generally encouraging relative to the sustained usefulness of fluconazole as a systemically active antifungal agent for the treatment of candida BSI. © 2004 Copyright by the European Society of Clinical Microbiology and Infectious Diseases.
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| 2. |
- Goetsch, L., et al.
(författare)
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Influence of administration dose and route on the immunogenicity and protective efficacy of BBG2Na, a recombinant respiratory syncytial virus subunit vaccine candidate
- 2000
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Ingår i: Vaccine. - 0264-410X .- 1873-2518. ; 18:24, s. 2735-2742
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Tidskriftsartikel (refereegranskat)abstract
- The immunogenicity and protective efficacy of BBG2Na, a novel recombinant respiratory syncytial virus subunit vaccine candidate, was assessed in BALB/c mice under various conditions of dose, administration route and number of immunisations. A single intra-peritoneal (i.p.) dose of 2 mu g, or two doses of 0.2 mu g, were sufficient to induce elevated RSV-A serum antibodies and sterilising lung protective immunity. Serum antibody titres were significantly boosted following second immunisations, but not a third. Of three routes of immunisation, i.p. induced the highest RSV-A antibody titres, followed in efficacy by the intramuscular (i.m.) and subcutaneous (s.c.) routes. Nonetheless, all three routes induced comparable and sterilising lung protection. In contrast, upper respiratory tract protection was observed only after i.p. vaccination, although significant viral titre reductions were evident following i.m. or s.c. immunisations. Interestingly, Pepscan analyses indicated that antibody epitope usage was highest in i.p. and lowest in i.m. immunised mice, respectively. Nonetheless, all routes resulted in antibody responses to known lung protective epitopes (protectopes). Thus, the prevention of serious lower respiratory tract disease, the principle goal of a RSV vaccine, but not URT infection, is dose dependent but unlikely to be influenced by the route of BBG2Na administration.
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| 3. |
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| 4. |
- Cano, F., et al.
(författare)
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Partial protection to respiratory syncytial virus (RSV) elicited in mice by intranasal immunization using live staphylococci with surface-displayed RSV-peptides
- 2000
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Ingår i: Vaccine. - 0264-410X .- 1873-2518. ; 18:24, s. 2743-2752
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Tidskriftsartikel (refereegranskat)abstract
- A live bacterial vaccine-delivery system based on the food-grade bacterium Staphylococcus carnosus was used for delivery of peptides from the G glycoprotein of human respiratory syncytial virus, subtype A (RSV-A). Three peptides, corresponding to the G protein amino acids, 144-159 (denoted G5), 190-203 (G9) and 171-188 (G4 S), the latter with four cysteine residues substituted for serines, were expressed by recombinant means as surface-exposed on three different bacteria, and their surface accessibility on the bacteria was verified by fluorescence-activated cell sorting (FACS). Intranasal immunization of mice with the live recombinant staphylococci elicited significant anti-peptide as well as anti-virus serum IgG responses of balanced IgG1/IgG2a isotype profiles, and upon viral challenge with 10(5) tissue culture infectious doses(50) (TCID50), lung protection was demonstrated for approximately half of the mice in the G9 and G4 S immunization groups. To our knowledge, this is the first study in which protective immunity to a viral pathogen has been evoked using food-grade bacteria as vaccine-delivery vehicles.
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| 5. |
- Hansson, M., et al.
(författare)
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General expression vectors for Staphylococcus carnosus enabled efficient production of the outer membrane protein A of Klebsiella pneumoniae
- 2002
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Ingår i: FEMS Microbiology Letters. - : Oxford University Press (OUP). - 0378-1097 .- 1574-6968. ; 210:2, s. 263-270
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Tidskriftsartikel (refereegranskat)abstract
- General expression vectors, designed for intracellular expression or secretion of recombinant proteins in the non-pathogenic Staphylococcus carnosus, were constructed. Both vector systems encode two different affinity tags, an upstream albumin binding protein and a downstream hexahistidyl peptide, and are furnished with cleavage sites for two site-specific proteases for optional affinity tag removal. To evaluate the novel vectors, the gene encoding the outer membrane protein A (OmpA) of Klebsiella pneumoniae was introduced into the vectors. Efficient production was demonstrated in both systems, although, as expected for OmpA fusions, somewhat better intracellularly, and the fusion proteins could be recovered as full-length products by affinity chromatography.
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| 6. |
- Nguyen, Son Tien, 1953-, et al.
(författare)
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Hole effective masses in 4H SiC
- 2000
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Ingår i: Physical Review B. Condensed Matter and Materials Physics. - 1098-0121 .- 1550-235X. ; 61:16
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Tidskriftsartikel (refereegranskat)abstract
- Optically detected cyclotron resonance (ODCR) at X-band frequency (~9.23 GHz) was used to study hole effective masses in 4H SiC. In addition to the known ODCR signal related to the cyclotron resonance (CR) of electrons we have observed an ODCR peak at a higher magnetic field, which is attributed to the CR of holes. In the vicinity of the maximum of the uppermost valence band, the constant energy surface can be considered as an ellipsoid with the principal axis along the c axis and the effective masses of the holes were determined as mh? = (0.66▒0.02)m0 and mh?=(1.75▒0.02)m0. The influence of the polaron coupling effect on the effective mass values in 4H SiC is discussed. ⌐ 2000 The American Physical Society.
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| 7. |
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| 8. |
- Nguyen, Son Tien, 1953-, et al.
(författare)
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The Carbon Vacancy Pair in 4H and 6H SiC
- 2000
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Ingår i: Materials Science Forum, Vol. 338 - 342. - : Trans Tech Publications. ; , s. 821-824
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Konferensbidrag (refereegranskat)abstract
- Electron paramagnetic resonance (EPR) was used to study defects in p-type 4H and 6H SiC irradiated with 2.5 MeV electrons at elevated temperatures (400 °C). After irradiation, an anisotropic EPR spectrum, labeled EI4, having monoclinic symmetry (C1h) and an effective spin S = 1 was observed in both 4H and 6H SiC. The same g-tensor with the principal values, gx = 2.0051, gy = 2.0038 and gz = 2.0029 was determined for the spectra in both polytypes. Here the z- and x-axis lie in the (1 1 2̄ 0) plane and the y-axis perpendicular to this plane. The angle between the principal z-axis and the c-axis is 54°. The fine structure parameters were determined as D = 3.28×10-2 cm-1 and 3.44×10-2 cm-1 for 6H and 4H SiC, respectively; E = 0.67×10-2 cm-1 for both polytypes. From the obtained 29Si hyperfine structure and spin Hamiltionian parameters, the defect can be identified as the pair of two carbon vacancies in the (1 1 2̄ 0) or equivalent planes.
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| 9. |
- Noterdaeme, J. M., et al.
(författare)
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Spatially resolved toroidal plasma rotation with ICRF on JET
- 2003
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Ingår i: Nuclear Fusion. - : IOP Publishing. - 0029-5515 .- 1741-4326. ; 43:4, s. 274-289
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Tidskriftsartikel (refereegranskat)abstract
- Plasmas heated by ICRF only in the JET tokamak show distinct structures in the toroidal rotation profile, with regions where domega/dr > 0 when the minority cyclotron resonance layer is far off-axis. The rotation is dominantly co-current with a clear off-axis maximum. There is only a slight difference between a high-field side (HFS) or a low-field side position of this resonance layer: the off-axis maximum in the rotation profile is modestly higher for the HFS position. This is in contrast to the predictions of theories that rely mainly on the effects arising from ICRF-driven fast ions to account for ICRF-induced plasma rotation. The differences due to the direction of the antenna spectrum (co- or counter-) are small. A more central deposition of the ICRF power in L-mode and operation in H-mode both lead to more centrally peaked profiles, both in the co-direction. Strong MHD modes brake the rotation and lead to overall flat rotation profiles.
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| 10. |
- Ståhl, Stefan, et al.
(författare)
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Staphylococcal surface display and its applications
- 2000
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Ingår i: International Journal of Medical Microbiology. - : Elsevier BV. - 1438-4221 .- 1618-0607. ; 290:7, s. 571-577
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Forskningsöversikt (refereegranskat)abstract
- Novel surface proteins can be introduced onto the bacterial cell surface by recombinant means. Here, we describe the development of such display systems for two food-grade bacteria, Staphylococcus carnosus and Staphylococcus xylosus, and present how such engineered bacteria can be used in different applications. A study will be described in which such staphylococci were employed as vaccine delivery vehicles to elicit protective antibody responses to respiratory syncytial virus (RSV). The use of surface-engineered staphylococci as novel microbial biocatalysts, as a new type of whole-cell diagnostic devices or for adsorption of metal ions with potential environmental or biosensor applications, will also be discussed.
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| 11. |
- Umeda, T., et al.
(författare)
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EPR and theoretical studies of positively charged carbon vacancy in 4H-SiC
- 2004
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Ingår i: Physical Review B. Condensed Matter and Materials Physics. - : American Physical Society. - 1098-0121 .- 1550-235X. ; 70:23
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Tidskriftsartikel (refereegranskat)abstract
- The carbon vacancy is a dominant defect in 4H-SiC, and the "E15" electron-paramagnetic-resonance (EPR) spectrum originates from positively charged carbon vacancies (VC+) at quasicubic sites. The observed state for EI5, however, has been attributed to a motional-averaged state with the C3v symmetry, and its true atomic structure has not been revealed so far. We here report low temperature (
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| 12. |
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