| 1. |
- Blom, M., et al.
(author)
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Human eosinophils express, relative to other circulating leukocytes, large amounts of secretory 14-kD phospholipase A2
- 1998
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In: Blood. - 1528-0020. ; 91:8, s. 3037-3043
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Journal article (peer-reviewed)abstract
- Human eosinophils perform several functions dependent on phospholipase A2 (PLA2) activity, most notably the synthesis of platelet-activating factor (PAF) and leukotriene C4 (LTC4). Several forms of PLA2 have been identified in mammalian cells. In the present study, the 14-kD, secretory form of PLA2 was detected in human eosinophils by immunocytochemical staining with the specific monoclonal antibody (MoAb) 4A1. In contrast, preparations of neutrophils, monocytes, lymphocytes, and basophils did not show detectable staining. With two MoAbs in a sandwich enzyme-linked immunosorbent assay (ELISA), large amounts of sPLA2 were detected in lysates of eosinophils, that were 20-fold to 100-fold higher than in the other circulating leukocytes (ie, neutrophils, basophils, monocytes, and lymphocytes). In addition, with a commercially available sPLA2 activity assay kit, we were able to show high activity of sPLA2 in human eosinophils relative to neutrophils. Investigations at the ultrastructural level showed that sPLA2 in eosinophils is mainly located in specific granules. Immunoelectron microscopy also visualized sPLA2 within phagosomes after addition of opsonized particles to the eosinophils. However, sPLA2 was not detected in the cell-free supernatants of activated eosinophils, in contrast to eosinophil-cationic protein (ECP), which colocalizes with sPLA2 in resting eosinophils. These findings warrant further studies into the role of sPLA2 in eosinophil function.
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| 2. |
- Leusen, JHW, et al.
(author)
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Disturbed interaction of p21-rac with mutated p67-phox causes chronic granulomatous disease
- 1996
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In: The Journal of experimental medicine. - : Rockefeller University Press. - 0022-1007 .- 1540-9538. ; 184:4, s. 1243-1249
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Journal article (peer-reviewed)abstract
- Chronic granulomatous disease (CGD) is characterized by the failure of phagocytic leukocytes to generate superoxide, needed for the intracellular killing of microorganisms. This is caused by mutations in any one of the four subunits of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase. In a rare, autosomal recessive form of CGD, a 67-kD cytosolic component of this enzyme (p67-phox) is missing. We here report on a patient with a mutation in the p67-phox gene that leads to expression of a nonfunctional p67-phox protein. The purified granulocytes of this patient failed to produce superoxide and contained about half of the normal amount of p67-phox. Analysis of the cDNA and genomic DNA of this patient showed that the patient is a compound heterozygote for a triplet nucleotide deletion in the p67-phox gene, predicting an in-frame deletion of lysine 58 in the p67-phox protein and a larger deletion of 11-13 kb in the other allele. Interestingly, the 58Lys deletion in p67-phox disrupts the interaction with p21-rac1, a ras-related protein involved in the activation of the NADPH oxidase. In contrast to normal neutrophils, in which p47-phox and p67-phox translocate to the plasma membrane upon cell activation, the cells of the patient did not show this translocation, indicating that an interaction between p67-phox and p21-rac1 is essential for translocation of these cytosolic proteins and activation of the NADPH oxidase. Moreover, this CGD patient represents the first case of disease caused by a disturbed binding of a ras-related protein to its target protein.
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| 5. |
- Atac, A, et al.
(author)
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Single-step link of the superdeformed band in Eu-143
- 1996
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In: ZEITSCHRIFT FUR PHYSIK A-HADRONS AND NUCLEI. - : SPRINGER VERLAG. - 0939-7922. ; 355:4, s. 343-344
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Journal article (other academic/artistic)abstract
- A discrete gamma-ray transition with an energy of 3360.6 keV deexciting the second lowest SD state in Eu-143 has been discovered. It carries 3.2 % of the full intensity of the band and feeds into a nearly spherical state which is above the I = 35/2((+)),
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| 6. |
- Axelsson, A, et al.
(author)
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Single-step link from yrast SD band in Eu-143
- 1997
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In: PROGRESS IN PARTICLE AND NUCLEAR PHYSICS. - : PERGAMON PRESS LTD. - 0146-6410. ; 38, s. 51-52
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Journal article (other academic/artistic)abstract
- A discrete gamma-ray transition from the yrast SD band to the region of near-yrast discrete normal-deformed states has been identified in Eu-143. It has an energy of 3360.6 keV and an intensity corresponding to 3.2% of the full intensity of the SD band an
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| 11. |
- Dahlberg, L., et al.
(author)
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Authors' reply
- 1995
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In: Annals of the Rheumatic Diseases. - : BMJ. - 0003-4967. ; 54:8, s. 685-685
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Journal article (peer-reviewed)
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| 12. |
- De Kerpel, Jan O A, et al.
(author)
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Theoretical study of the structural and spectroscopic properties of stellacyanin
- 1998
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In: The Journal of Physical Chemistry Part B. - : American Chemical Society (ACS). - 1520-5207 .- 1520-6106. ; 102:23, s. 4638-4647
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Journal article (peer-reviewed)abstract
- The electronic spectrum of the azurin Met121Gln mutant, a model of the blue copper protein stellacyanin, has been studied by ab initio multiconfigurational second-order perturbation theory (the CASPT2 method), including the effect of the protein and solvent by point charges. The six lowest electronic transitions have been calculated and assigned with an error of less than 2400 cm-1. The ground-state singly occupied orbital is found to be a predominantly π antibonding orbital involving Cu3d and Scys3pπ. However, it also contains a significant amount (18%) of Cu-Scys σ antibonding character. This σ interaction is responsible for the appearance in the absorption spectrum of a band at 460 nm, with a significantly higher intensity than observed for other, axial, type 1 copper proteins (i.e., plastocyanin or azurin). The π-σ mixing is caused by the axial glutamine ligand binding at a much shorter distance to copper than the corresponding methionine ligand in the normal blue copper proteins. This explains why, based on its spectral properties, stellacyanin is classified among the rhombic type 1 copper proteins, although its structure is clearly trigonal, as it is for the axial proteins. Calculations have also been performed on structures where the glutamine model coordinates to the copper ion via the deprotonated N∈ atom instead of the O∈ atom. However, the resulting transition energies do not resemble the experimental spectrum obtained at normal or elevated pH. Thus, the results do not confirm the suggestion that the coordinating atom of glutamine changes at high pH.
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| 13. |
- Frisén, Lars, 1939, et al.
(author)
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Surgically created fourth-third cranial nerve communication: temporary success in a child with bilateral third nerve hamartomas. Case report.
- 1999
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In: Journal of neurosurgery. - : Journal of Neurosurgery Publishing Group (JNSPG). - 0022-3085. ; 90:3, s. 542-5
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Journal article (peer-reviewed)abstract
- Shortly after birth, an otherwise healthy infant developed eye deviation and ptosis due to a hamartomatous lesion of the interpeduncular segment of the right oculomotor nerve. The left nerve became similarly involved when the child was 1.5 years of age. Direct nerve repair was not possible. Instead, the trochlear nerve was divided and its proximal end was attached to the distal end of the third nerve. Elevation of the upper eyelid and partial adduction of the eye developed gradually over the ensuing 3 to 5 months. Both functions were lost after an additional 2 months, presumably as a result of tumor recurrence or neuroma formation. This case report shows that surgically created fourth-third cranial nerve communication is feasible and may merit consideration under similar circumstances.
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| 17. |
- Nostell, P, et al.
(author)
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Ageing of solar booster reflector materials
- 1998
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In: SOLAR ENERGY MATERIALS AND SOLAR CELLS. - : ELSEVIER SCIENCE BV. - 0927-0248. ; 54:1-4, s. 235-246
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Journal article (other academic/artistic)abstract
- Anodized aluminium lacquered with polyvinylidene fluoride, anodized aluminium, vacuum evaporated aluminium foil and stainless steel have been aged up to 7 yr. It was found that anodized aluminium is not long-term durable, while the others show only slow d
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| 22. |
- Nostell, P, et al.
(author)
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Optical and mechanical properties of sol-gel antireflective films for solar energy applications
- 1999
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In: THIN SOLID FILMS. - : ELSEVIER SCIENCE SA. - 0040-6090. ; 351:1-2, s. 170-175
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Journal article (other academic/artistic)abstract
- Antireflective films on glass were prepared by a dip-coating method from a sol-gel, which consisted of 50 nm monodisperse silica particles mixed with ethanol and water. The solar transmittance increased by 5.4% for the best made sample. A visible transmit
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