Sökning: WFRF:(Ruel Marc)
> (2006-2009) >
Tissue-engineered i...
Tissue-engineered injectable collagen-based matrices for improved cell delivery and vascularization of ischemic tissue using CD133+progenitors expanded from the peripheral blood
-
- Suuronen, Erik J. (författare)
- Division of Cardiac Surgery, University of Ottawa, Ottawa, Ontario, Canada
-
- Veinot, John P. (författare)
- Department of Pathology and Laboratory Medicine, University of Ottawa, Ottawa, Ontario, Canada
-
- Wong, Serena (författare)
- Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada
-
visa fler...
-
- Kapila, Varun (författare)
- Division of Cardiac Surgery, University of Ottawa, Ottawa, Ontario, Canada
-
- Price, Joel (författare)
- Division of Cardiac Surgery, University of Ottawa, Ottawa, Ontario, Canada
-
- Griffith, May (författare)
- Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada
-
- Mesana, Thierry G. (författare)
- Division of Cardiac Surgery, University of Ottawa, Ottawa, Ontario, Canada
-
- Ruel, Marc (författare)
- Division of Cardiac Surgery and Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, Ontario, Canada
-
visa färre...
-
(creator_code:org_t)
- LIPPINCOTT WILLIAMS and WILKINS, 2006
- 2006
- Engelska.
-
Ingår i: Circulation. - : LIPPINCOTT WILLIAMS and WILKINS. - 0009-7322 .- 1524-4539. ; 114, s. I138-I144
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Background-The use of stem and/or progenitor cells to achieve potent vasculogenesis in humans has been hindered by low cell numbers, implant capacity, and survival. This study investigated the expansion of CD133(+) cells and the use of an injectable collagen-based tissue engineered matrix to support cell delivery and implantation within target ischemic tissue. Methods and Results-Adult human CD133(+) progenitor cells from the peripheral blood were generated and expanded by successive removal and culture of CD133(-) cell fractions, and delivered within an injectable collagen-based matrix into the ischemic hindlimb of athymic rats. Controls received injections of phosphate-buffered saline, matrix, or CD133(+) cells alone. Immunohistochemistry of hindlimb muscle 2 weeks after treatment revealed that the number of CD133(+) cells retained within the target site was greater than 2-fold greater when delivered by matrix than when delivered alone (P less than 0.01). The transplanted CD133(+) cells incorporated into vascular structures, and the matrix itself also was vascularized. Rats that received matrix and CD133(+) cells demonstrated greater intramuscular arteriole and capillary density than other treatment groups (P less than 0.05 and P less than 0.01, respectively). Conclusions-Compared with other experimental approaches, treatment of ischemic muscle tissue with generated CD133(+) progenitor cells delivered in an injectable collagen-based matrix significantly improved the restoration of a vascular network. This work demonstrates a novel approach for the expansion and delivery of blood CD133(+) cells with resultant improvement of their implantation and vasculogenic capacity.
Nyckelord
- angiogenesis; cells; endothelial progenitor cells; ischemia; revascularization; tissue engineering
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas