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- Kato, S, et al.
(författare)
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DNA hypermethylation and inflammatory markers in incident Japanese dialysis patients
- 2012
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Ingår i: Nephron extra. - : S. Karger AG. - 1664-5529. ; 2:1, s. 159-68
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Tidskriftsartikel (refereegranskat)abstract
- <b><i>Background/Aims:</i></b> Inflammation is an established mortality risk factor in chronic kidney disease (CKD) patients. Although a previous report showed that uremic Caucasian patients with inflammation had signs of global DNA hypermethylation, it is still unknown whether DNA hypermethylation is linked to inflammatory markers including a marker of bacterial infections in Japanese CKD patients. <b><i>Methods:</i></b> In 44 consecutive incident dialysis patients (26 males, mean age 59 ± 12 years) without clinical signs of infection, global DNA methylation was evaluated in peripheral blood DNA using the <i>Hpa</i>II<i>/Msp</i>I ratio by the luminometric methylation assay method. A lower ratio of <i>Hpa</i>II<i>/Msp</i>I indicates global DNA hypermethylation. Procalcitonin (PCT), a marker of inflammation due to bacterial infections, was measured using an immunochromatographic assay. <b><i>Results:</i></b> The patients were divided into hyper- and hypomethylation groups based on the median value of the <i>Hpa</i>II<i>/Msp</i>I ratio 0.31 (range 0.29–0.37). Whereas patients in the hypermethylation group had higher ferritin levels [133.0 (51.5–247.3) vs. 59.5 (40.0–119.0) ng/ml; p = 0.046], there were no significant differences in age, gender, diabetes, smoking, anemia or serum albumin levels. However, the <i>Hpa</i>II<i>/Msp</i>I ratio showed significant negative correlations with PCT (ρ = –0.32, p = 0.035) and ferritin (ρ = –0.33, p = 0.027) in Spearman’s rank test. In a multiple linear regression analysis, PCT and ferritin were associated with a lower <i>Hpa</i>II<i>/Msp</i>I ratio (R<sup>2</sup> = 0.24, p = 0.013). <b><i>Conclusion:</i></b> In this study, global DNA hypermethylation was associated with ferritin and, most likely, PCT, suggesting that inflammation induced by subclinical bacterial infection promoted DNA methylation.
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| 2. |
- Nakajima, Y., et al.
(författare)
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Approach to 3D dose verification by utilizing autoactivation
- 2011
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Ingår i: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment. - : Elsevier BV. - 0168-9002. ; 648 (Supplement 1):SUPPL. 1, s. S119-S121
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Tidskriftsartikel (refereegranskat)abstract
- To evaluate the deposited dose distribution in a target, we have proposed to utilize the annihilation gamma-rays emitted from the positron emitters distributed in the target irradiated with stable heavy-ion beams. Verification of the one dimensional (1-D) dose distributions along and perpendicular to a beam axis was achieved through our previous works. The purpose of this work is to verify 3-D dose distributions. As the first attempt uniform PMMA targets were irradiated in simple rectangular parallelepiped shapes, and the annihilation gamma-rays were detected with a PET scanner. By comparing the detected annihilation gamma-ray distributions with the calculated ones the dose distributions were estimated. As a result the estimated positions of the distal edges of the dose distributions were in agreement with the measured ones within 1 mm. However, the estimated positions of the proximal edges were different from the measured ones by 5-9 mm depending on the thickness of the irradiation filed.
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