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- Klemmensen, B., et al.
(författare)
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Refinery Sector
- 1999
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Ingår i: Policies for Cleaner Technology. - London : Earthscan Publications Ltd.. - 9781853835193 - 1853835188 ; , s. 52-72
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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- Baas, Leenard W., et al.
(författare)
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Petro-chemical Sector
- 1999
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Ingår i: Policies for Cleaner Technology. - London : Earthscan Publications Ltd.. - 9781853835193 - 1853835188 ; , s. 73-90
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Bokkapitel (refereegranskat)
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- Huckvale, M., et al.
(författare)
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Opportunities for Computer-Aided Instruction in Phonetics and Speech Communication Provided by the Internet
- 1997
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Ingår i: 5th European Conference on Speech Communication and Technology, EUROSPEECH 1997. - : The International Speech Communication Association (ISCA). ; , s. 1919-1922
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Konferensbidrag (refereegranskat)abstract
- Spoken language engineering is starting to deliver technological products to the commercial market and has an important future role in supporting the multilingual structures of modern Europe. The field will be driven forward by basic science and applied research by experts drawn from a variety of backgrounds; among them: linguistics, psychology, computer science and electrical engineering. The wide range of expertise required in this discipline brings difficulties for our educational systems, but also challenges us to use our knowledge of technology and communication to improve the quality and effectiveness of teaching and learning. This paper investigates how resources currently available on the Internet could be exploited in the education of phonetics and speech communication. It discusses the technology, outlines the requirements for computer-aided learning in the field, gives a taxonomy of the available components with examples, and criticises the main weaknesses in the current provision.
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- Milton, Debra L., et al.
(författare)
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Quorum sensing in Vibrio anguillarum : characterization of the vanI/vanR locus and identification of the autoinducer N-(3-oxodecanoyl)-L-homoserine lactone
- 1997
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Ingår i: Journal of Bacteriology. - 0021-9193 .- 1098-5530. ; 179:9, s. 3004-3012
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Tidskriftsartikel (refereegranskat)abstract
- Certain gram-negative pathogens are known to control virulence gene expression through cell-cell communication via small diffusible signal molecules termed autoinducers. This intercellular signal transduction mechanism termed quorum sensing depends on the interaction of an N-acylhomoserine lactone (AHL) auto-inducer molecule with a receptor protein belonging to the LuxR family of positive transcriptional activators. Vibrio anguillarum is a gram-negative pathogen capable of causing a terminal hemorrhagic septicemia known as vibriosis in fish such as rainbow trout. In this study, we sought to determine whether V. anguillarum employs AHLs to regulate virulence gene expression. Spent V. anguillarum culture supernatants stimulated bioluminescence in a recombinant lux-based Escherichia coli AHL biosensor strain, whereas they both stimulated and inhibited AHL-mediated violacein pigment production in Chromobacterium violaceum. This finding suggested that V. anguillarum may produce multiple AHL signal molecules. Using high-performance liquid chromatography and high-resolution tandem mass spectrometry, we identified the major V. anguillarum AHL as N-(3-oxodecanoyl)-L-homoserine lactone (ODHL), a structure which was unequivocally confirmed by chemical synthesis. The gene (vanI) responsible for ODHL synthesis was cloned and sequenced and shown to belong to the LuxI family of putative AHL synthases. Further sequencing downstream of vanI revealed a second gene (vanR) related to the LuxR family of transcriptional activators. Although deletion of vanI abolished ODHL synthesis, no reduction of either metalloprotease production or virulence in a fish infection model was observed. However, the vanI mutant remained capable of weakly activating both bioluminescence and violacein in the E. coli and C. violaceum biosensors, respectively, indicating the existence of additional layers of AHL-mediated regulatory complexity.
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