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All-trans retinoic ...
All-trans retinoic acid prevents epidural fibrosis through NF-kappa B signaling pathway in post-laminectomy rats
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Zhang, Chao (författare)
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Kong, Xiaohong (författare)
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Ning, Guangzhi (författare)
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Liang, Zhipin (författare)
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Qu, Tongjun (författare)
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Chen, Feiran (författare)
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Cao, Daigui (författare)
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Wang, Tianyi (författare)
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- Sharma, Hari Shanker (författare)
- Uppsala universitet,Anestesiologi och intensivvård
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Feng, Shiqing (författare)
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(creator_code:org_t)
- Elsevier BV, 2014
- 2014
- Engelska.
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Ingår i: Neuropharmacology. - : Elsevier BV. - 0028-3908 .- 1873-7064. ; 79C, s. 275-281
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Laminectomy is a widely accepted treatment for lumbar disorders, and epidural fibrosis (EF) is a common complication. EF is thought to cause post-operative pain recurrence after laminectomy or discectomy. All-trans retinoic acid (ATRA) has shown anti-fibrotic, anti-inflammatory, and anti-proliferative functions. The object of this study was to investigate the effects of ATRA on the prevention of EF in post-laminectomy rats. In vitro, the anti-fibrotic effect of ATRA was demonstrated with cultured fibroblasts count, which comprised of those that were cultured with/without ATRA. In vivo, rats underwent laminectomy at the L1-L2 levels. We first demonstrated the beneficial effects using 0.05% ATRA compared to vehicle (control group). We found that a higher concentration of ATRA (0.1%) achieved dose-dependent results. Hydroxyproline content, Rydell score, vimentin-positive cell density, fibroblast density, inflammatory cell density and inflammatory factor expression levels all suggested better outcomes in the 0.1% ATRA rats compared to the other three groups. Presumably, these effects involved ATRA's ability to suppress transforming growth factor (TGF-β1) and interleukin (IL)-6 which was confirmed with reverse-transcriptase polymerase chain reaction (RT-PCR). Finally we demonstrated that ATRA down-regulated nuclear factor (NF)-κB by immunohistochemistry and western blotting for p65 and inhibition of κB (IκBα), respectively. Our findings indicate that topical application of ATRA can inhibit fibroblast proliferation, decrease TGF-β1 and IL-6 expression level, and prevent epidural scar adhesion in rats. The highest concentration employed in this study (0.1%) was the most effective. ATRA suppressed EF through down-regulating NF-κB signaling, whose specific mechanism is suppression of IκB phosphorylation and proteolytic degradation.
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Zhang, Chao
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Kong, Xiaohong
-
Ning, Guangzhi
-
Liang, Zhipin
-
Qu, Tongjun
-
Chen, Feiran
-
visa fler...
-
Cao, Daigui
-
Wang, Tianyi
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Sharma, Hari Sha ...
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Feng, Shiqing
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visa färre...
- Artiklar i publikationen
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Neuropharmacolog ...
- Av lärosätet
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Uppsala universitet