| 1. |
|
|
| 2. |
|
|
| 3. |
|
|
| 4. |
- Giwercman, YL, et al.
(författare)
-
Response to treatment in patients with partial androgen insensitivity due to mutations in the DNA-binding domain of the androgen receptor
- 2000
-
Ingår i: Hormone research. - : S. Karger AG. - 0301-0163. ; 53:2, s. 83-88
-
Tidskriftsartikel (refereegranskat)abstract
- The androgen insensitivity syndrome is a disorder caused by deficient function of the androgen receptor, characterized by varying degrees of undermasculinization in karyotypic males. We have identified four mutations in the androgen receptor gene, in the region encoding the DNA-binding domain of the protein. Two mutations, R607X and R615G, were found in patients with complete insensitivity to androgens, whereas the other two, S578T and A596T, were found in patients with partial insensitivity. The functional consequences of the three missense mutations were assayed in vitro after transient expression of the receptors in COS cells. All mutants showed normal androgen binding but abnormal abilities to stimulate transcription of an androgen-responsive reporter gene. R615G abolished transactivation whereas S578T and A596T were partially malfunctional. The function of A596T, but not of S578T, was normalized at high androgen concentrations in vitro, reflecting the in vivo situation. Thus, patients with specific mutations in the DNA-binding domain of the androgen receptor may benefit from androgen treatment.
|
|
| 5. |
- Landstrom, U, et al.
(författare)
-
Effect on truck drivers' alertness of a 30-min. exposure to bright light: a field study
- 2004
-
Ingår i: Perceptual and motor skills. - : SAGE Publications. - 0031-5125 .- 1558-688X. ; 98:33 Pt 1, s. 770-776
-
Tidskriftsartikel (refereegranskat)abstract
- Reduced alertness is common during night driving. Light treatment may constitute one countermeasure to reduce sleepiness. To test this idea six professional drivers participated in this study in which they self-administered a 30-min. light treatment during a break in the middle of a night drive of about 9 hours. Two experimental conditions were used, including light exposures with a light box and a light visor. There was a control condition. Alertness was measured on a 100-mm visual analogue scale. No significant effect of light was found, but ratings of sleepiness increased significantly through the night drive. The experimental light treatment was not correlated with any increased wakefulness compared to the drivings where no extra light exposures were carried out.
|
|
| 6. |
|
|
| 7. |
- Tornblom, SA, et al.
(författare)
-
15-Hydroxyprostaglandin dehydrogenase and cyclooxygenase 2 messenger ribonucleic acid expression and immunohistochemical localization in human cervical tissue during term and preterm labor
- 2004
-
Ingår i: Journal of Clinical Endocrinology and Metabolism. - : The Endocrine Society. - 1945-7197 .- 0021-972X. ; 89:6, s. 2909-2915
-
Tidskriftsartikel (refereegranskat)abstract
- Here we have examined the enzymes cyclooxygenase (COX)-2 and 15-hydroxyprostaglandin dehydrogenase (15-OH PGDH) in pregnant human cervix. In biopsies taken transvaginally after preterm and term elective cesarean sections and vaginal deliveries, the levels of mRNA coding for COX-2 and 15-OH PGDH were assessed by Northern blotting. The cellular localization of the COX-2 and 15-OH PGDH proteins was determined by immunohistochemical analysis. COX-2 and 15-OH PGDH mRNAs were expressed at detectable levels in the cervical biopsies from all four groups of subjects. At cesarean sections ( unripe cervix), the level of 15-OH PGDH mRNA was significantly higher than the level in the ripe cervix at the time of partus, irrespective of the gestational length. In contrast, the level of COX-2 mRNA was similar in all subjects. Immunoreactivity of COX-2 and 15-OH PGDH was expressed by activated fibroblasts. The present investigation documents the expression and cellular localization of COX-2 and 15-OH PGDH in the preterm and term pregnant human cervix. This observation indicates that both preterm and term cervical ripening is associated with decreased degradation of prostaglandins.
|
|
| 8. |
|
|
| 9. |
- Westergren Soderberg, M, et al.
(författare)
-
Young women with genital prolapse have a low collagen concentration
- 2004
-
Ingår i: Acta Obstetricia et Gynecologica Scandinavica. - : Wiley. - 1600-0412 .- 0001-6349. ; 83:12, s. 1193-1198
-
Tidskriftsartikel (refereegranskat)abstract
- Background. Genital prolapse is a common and handicapping form of pelvic floor dysfunction. To explain its genesis as a result of endopelvic connective tissue weakness, the collagen state was analyzed in women with and without genital prolapse. Methods. Punch biopsies from the paraurethral ligaments were obtained during the operation from 22 women undergoing surgery for genital prolapse. As controls, similar biopsies were taken from 13 women who underwent gynecologic surgery for other benign reasons. Collagen concentration as hydroxyproline and its extractability by pepsin digestion were studied in relation to age by multiple regression, two-way ANOVA, Levene's test, and Student's t-test. Histological examination was also performed. Results. Women, younger than 53 years, with genital prolapse had a 30% lower collagen concentration than age-matched controls, which reached significance, P = 0.01. The extractability by pepsin digestion, an indicator of cross-links in the collagen molecule, did not significantly differ between groups. It did, however, decrease significantly with age in both prolapse patient and control groups. Morphology supported these findings with a less-dense extracellular matrix composition subepithelially in genital prolapse compared to a healthy control. Conclusion. For the first time, we show that young women with genital prolapse have a decreased collagen concentration, suggesting a different organization of the endopelvic connective tissue extracellular matrix. Furthermore, these alterations differ from those earlier found in younger women with stress urinary incontinence.
|
|
| 10. |
|
|
