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- Kepka, C., et al.
(author)
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Pilot-scale extraction of an intracellular recombinant cutinase from E-coli cell homogenate using a thermoseparating aqueous two-phase system
- 2003
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In: Journal of Biotechnology. - : Elsevier BV. - 0168-1656 .- 1873-4863. ; 103:2, s. 165-181
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Journal article (peer-reviewed)abstract
- A thermoseparating aqueous two-phase system for extraction of a recombinant cutinase fusion protein from Escherichia coli homogenate has been scaled up to pilot scale. The target protein ZZ-cutinase-(WP)(4) was produced in a fed batch process at 500 1 to a concentration of 12% of the total protein and at a cell concentration of 19.7 g l(-1). After harvest and high-pressure homogenisation a first extraction step was performed in an EO50PO50 (50% (w/w) ethylene oxide and 50% (w/w) propylene oxide) thermopolymer/amylopectin rich Waxy barley starch system. The (WP)4 tag was used for enhanced target protein partitioning to the EO50PO50 phase while the cell debris was collected in the starch phase. A second extraction step followed where the recovered EO50PO50 phase from the first step was supplemented with a non-ionic detergent (C12-18EO5) and heated to the cloud point (CP) temperature (45 degreesC). One polymer-rich liquid phase and one almost pure aqueous phase were formed. The target protein could be obtained in a water phase after the thermal phase separation at a total recovery over the extraction steps of 71% and a purification factor of 2.5. We were able to demonstrate that a disk-stack centrifugal separator could be adapted for rapid separation of both primary and thermoseparated phase systems.
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- Nilsson, E, et al.
(author)
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Electrochemical treatment of tumours
- 2000
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In: Bioelectrochemistry (Amsterdam, Netherlands). - 1567-5394. ; 51:1, s. 1-11
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Journal article (peer-reviewed)
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- Wallenius, Janne, 1968-, et al.
(author)
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Development of an EAM potential for simulation of radiation damage in Fe-Cr alloys
- 2004
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In: Journal of Nuclear Materials. - : Elsevier BV. - 0022-3115 .- 1873-4820. ; 329-33, s. 1175-1179
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Journal article (peer-reviewed)abstract
- We have developed a set of EAM potentials for simulation of Fe-Cr alloys. By relaxing the requirement of reproducing the pressure-volume relation at short distances and by fitting to the thermal expansion coefficients of Fe and Cr, stability of theself-interstitial could be obtained. For Cr, properties of the paramagnetic state were applied, providing a positive Cauchy pressure. Mixed Fe-Cr pair potentials were fitted to the calculated mixing enthalpy of ferromagnetic Fe-Cr. Simulation of thermal ageing in Fe-Cr alloys using the Fe-20Cr potential exhibited pronounced Cr-precipitation for temperatures below 900 K, a feature not observed at any temperature using a potential fitted to the mixing enthalpy of Fe-5Cr.
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