| 1. |
- Hussain Ibupoto, Zafar, et al.
(författare)
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The Development of Highly Sensitive and Selective Immunosensor Based on Antibody Immobilized ZnO Nanorods for the Detection of D-Dimer
- 2014
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Ingår i: Electroanalysis. - : Wiley-VCH Verlag. - 1040-0397 .- 1521-4109. ; 26:2, s. 292-298
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Tidskriftsartikel (refereegranskat)abstract
- ZnO nanorods were grown on gold coated glass substrate by low temperature aqueous chemical growth method. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques were used for the characterization of ZnO nanorods. ZnO nanorods are highly dense, uniform, well aligned and perpendicular-oriented to the substrate. ZnO nanorods exhibited good crystal quality. The well aligned ZnO nanorods were potentially used for the development of selective and sensitive immunosensor for the detection of D-dimer by immobilizing antibody on stabilized lipid films. The ZnO nanorods based immunosensor responded to a wide range of D-dimer concentrations with fast response time of ca. 20s.
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| 2. |
- Nikoleli, Georgia-Paraskevi, et al.
(författare)
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Potentiometric cholesterol biosensing application of graphene electrode with stabilized polymeric lipid membrane
- 2013
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Ingår i: Central European Journal of Chemistry. - : Springer Verlag (Germany). - 1895-1066 .- 1644-3624. ; 11:9, s. 1554-1561
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Tidskriftsartikel (refereegranskat)abstract
- A novel potentiometric cholesterol biosensor has been fabricated through the immobilization of the stabilized polymeric lipid membrane onto graphene electrode. The stabilized polymeric lipid membrane is composed of cholesterol oxidase enzyme and polymerization mixture; which holds paramount influence on the properties of the cholesterol biosensor. The presented biosensor reveals an appreciable reproducibility, good selectivity and high sensing capability with a linear slope curve of similar to 64 mV per decade. The strong biocompatibility among stabilized polymeric lipid membranes and human biofluids provides the possibility to use for real blood samples and other biological applications.
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| 3. |
- Nikoleli, Georgia-Paraskevi, et al.
(författare)
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Structural Characterization of Graphene Nanosheets for Miniaturization of Potentiometric Urea Lipid Film Based Biosensors
- 2012
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Ingår i: Electroanalysis. - : Wiley-VCH Verlag Berlin. - 1040-0397 .- 1521-4109. ; 24:6, s. 1285-1295
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Tidskriftsartikel (refereegranskat)abstract
- The present article describes a miniaturized potentiometric urea lipid film based biosensor on graphene nanosheets. Structural characterization of graphene nanosheets for miniaturization of potentiometric urea lipid film based biosensors have been studied through atomic force microscopy (AFM) and transmission electron microscopy (TEM) measurements. UV-Vis and Fourrier transform IR (FTIR) spectroscopy have been utilized to study the pre- and postconjugated surfaces of graphene nanosheets. The presented potentiometric urea biosensor exhibits good reproducibility, reusability, selectivity, rapid response times (similar to 4 s), long shelf life and high sensitivity of ca. 70 mV/decade over the urea logarithmic concentration range from 1 x 10(-6) M to 1 x 10(-3) M.
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| 4. |
- Psychoyios, Vasillios N., et al.
(författare)
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Potentiometric Cholesterol Biosensor Based on ZnO Nanowalls and Stabilized Polymerized Lipid Film
- 2013
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Ingår i: Electroanalysis. - : John Wiley & Sons. - 1040-0397 .- 1521-4109. ; 25:2, s. 367-372
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Tidskriftsartikel (refereegranskat)abstract
- A novel potentiometric cholesterol biosensor was fabricated by immobilization of cholesterol oxidase into stabilized lipid films using zinc oxide (ZnO) nanowalls as measuring electrode. Cholesterol oxidase was incorporated into the lipid film prior polymerization on the surface of ZnO nanowalls resulting in a sensitive, selective, stable and reproducible cholesterol biosensor. The potentiometric response was 57mV/ decade concentration. The sensor response had no interferences by normal concentrations of ascorbic acid, glucose, and urea, proteins and lipids. The present biosensor could be implanted in the human body because of the biocompatibility of the lipid film.
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| 5. |
- Tzamtzis, Nikolaos, et al.
(författare)
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Flow Potentiometric Injection Analysis of Uric Acid Using Lipid Stabilized Films with Incorporated Uricase on ZnO Nanowires
- 2012
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Ingår i: Electroanalysis. - : Wiley-VCH Verlag Berlin. - 1040-0397 .- 1521-4109. ; 24:8, s. 1719-1725
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Tidskriftsartikel (refereegranskat)abstract
- A novel potentiometric uric acid biosensor was fabricated by immobilization of uricase into stabilized lipid films using zinc oxide (ZnO) nanowires as measuring electrode. Uricase was incorporated into the lipid film prior polymerization on the surface of well aligned ZnO nanowires resulting in a sensitive, selective, stable and reproducible uric acid biosensor. The potentiometric response was twice as large from previously reported values due to the presence of a cationic lipid in the lipid film. The sensor response had no interferences by normal concentrations of ascorbic acid, glucose, urea, proteins and lipids.
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