| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
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| 3. |
- Heuzé, Céline, 1988, et al.
(författare)
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Spaceborne infrared imagery for early detection of Weddell Polynya opening
- 2021
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Ingår i: Cryosphere. - : Copernicus GmbH. - 1994-0416. ; 15:7, s. 3401-3421
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Tidskriftsartikel (refereegranskat)abstract
- Knowing when sea ice will open is crucial, notably for scientific deployments. This was particularly obvious when the Weddell Polynya, a large opening in the winter Southern Ocean sea ice, unexpectedly re-appeared in 2016. As no precursor had been detected, observations were limited to chance autonomous sensors, and the exact cause of the opening could not be determined accurately. We investigate here whether the signature of the vertical ocean motions or that of the leads, which ultimately re-open the polynya, are detectable in spaceborne infrared temperature before the polynya opens. From the full historical sea ice concentration record, we find 30 polynyas starting from 1980. Then, using the full time series of the spaceborne infrared Advanced Very High Resolution Radiometer, we determine that these events can be detected in the 2 weeks before the polynya opens as a reduction in the variance of the data. For the three commonly used infrared brightness temperature bands, the 15 d sum and 15 d standard deviation of their area median and maximum are systematically lower than the climatology when a polynya will open. Moreover, by comparing the infrared brightness temperature to atmospheric reanalysis, hydrographic mooring data, and autonomous profilers, we find that temporal oscillations in one band and the decrease in the difference between bands may be used as proxies for upwelling of warm water and presence of leads, respectively, albeit with caution. Therefore, although infrared data are strongly limited by their horizontal resolution and sensitivity to clouds, they could be used for studying ocean or atmosphere preconditioning of polynyas in the historical record.
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| 4. |
- Lu, Xi, et al.
(författare)
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Luminescent solar concentrator efficiency versus edge solar cell coverage
- 2023
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Ingår i: Optics Letters. - : Optica Publishing Group. - 0146-9592 .- 1539-4794. ; 48:16, s. 4197-4200
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Tidskriftsartikel (refereegranskat)abstract
- This Letter introduces an analytical approach to estimate the waveguiding efficiency of large-area luminescent solar concentrators (LSCs), where the edges are covered by a var-ied number of mirrors and solar cells. The model provides physically relevant description in the whole range of optical (absorption, scattering) and geometrical (size) parameters of rectangular LSCs. A 19 x 19 cm2 silicon quantum dot -based LSC has been fabricated to verify the theory. Within an experimental error, the predicted waveguiding efficiency matched well the measured one. A critical LSC size, beyond which a part of the device turns inactive, has been deter-mined as N/& alpha; for N attached solar cells (one or two) and LSC material absorption coefficient & alpha;. This model provides a straightforward waveguiding analysis tool for large-area LSCs with different structural parameters relevant for both high concentration ratio and glazing applications.
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| 5. |
- Willatt, Rosemary, et al.
(författare)
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Retrieval of Snow Depth on Arctic Sea Ice From Surface-Based, Polarimetric, Dual-Frequency Radar Altimetry
- 2023
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Ingår i: Geophysical Research Letters. - 0094-8276 .- 1944-8007. ; 50:20
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Tidskriftsartikel (refereegranskat)abstract
- Snow depth on sea ice is an Essential Climate Variable and a major source of uncertainty in satellite altimetry-derived sea ice thickness. During winter of the MOSAiC Expedition, the “KuKa” dual-frequency, fully polarized Ku- and Ka-band radar was deployed in “stare” nadir-looking mode to investigate the possibility of combining these two frequencies to retrieve snow depth. Three approaches were investigated: dual-frequency, dual-polarization and waveform shape, and compared to independent snow depth measurements. Novel dual-polarization approaches yielded r2 values up to 0.77. Mean snow depths agreed within 1cm, even for data sub-banded to CryoSat-2 SIRAL and SARAL AltiKa bandwidths. Snow depths from co-polarized dual-frequency approaches were at least a factor of four too small and had a r2 0.15 or lower. r2 for waveform shape techniques reached 0.72 but depths were underestimated. Snow depth retrievals using polarimetric information or waveform shape may therefore be possible from airborne/satellite radar altimeters.
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| 6. |
- Zhang, Q., et al.
(författare)
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Mechanical property and reliability of bimodal nano-silver paste with Ag-coated SiC particles
- 2019
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Ingår i: Soldering and Surface Mount Technology. - 1758-6836 .- 0954-0911. ; 31:4, s. 193-202
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Tidskriftsartikel (refereegranskat)abstract
- © 2019, Emerald Publishing Limited. Purpose: This study aims to develop a bimodal nano-silver paste with improved mechanical property and reliability. Silicon carbide (SiC) particles coated with Ag were introduced in nano-silver paste to improve bonding strength between SiC and Ag particles and enhance high-temperature stability of bimodal nano-silver paste. The effect of sintering parameters such as sintering temperature, sintering time and the proportion of SiC particles on mechanical property and reliability of sintered bimodal nano-silver structure were investigated. Design/methodology/approach: Sandwich structures consist of dummy chips and copper substrates with nickel and silver coating bonded by nano-silver paste were designed for shear testing. Shear strength testing was conducted to study the influence of SiC particles proportions on the mechanical property of sintered nano-silver joints. The reliability of the bimodal nano-silver paste was evaluated experimentally by means of shear test for samples subjected to thermal aging test at 150°C and humidity and temperature testing at 85°C and 85 per cent RH, respectively. Findings: Shear strength was enhanced obviously with the increase of sintering temperature and sintering time. The maximum shear strength was achieved for nano-silver paste sintered at 260°C for 10 min. There was a negative correlation between the proportion of SiC particles and shear strength. After thermal aging testing and humidity and temperature testing for 240 h, the shear strength decreased a little. High-temperature stability and high-hydrothermal stability were improved by the addition of SiC particles. Originality/value: Submicron-scale SiC particles coated with Ag were used as alternative materials to replace part of nano-silver particles to prepare bimodal nano-silver paste due to its high thermal conductivity and excellent mechanical property.
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| 7. |
- Zhou, Lu, 1993, et al.
(författare)
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Early Winter Triggering of the Maud Rise Polynya
- 2022
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Ingår i: Geophysical Research Letters. - 0094-8276 .- 1944-8007. ; 49:2
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Tidskriftsartikel (refereegranskat)abstract
- What triggers Maud Rise polynya, a large opening in the winter Antarctic sea ice, is still debated. We show that the upcoming opening of all Maud Rise polynyas can be detected in early winter up to four months ahead, especially since the 2002 expansion in satellite observations. In all polynya years, continuous anomalous sea ice thinning begins in May, caused by atmospheric and oceanic forcings. Dynamically, an anomalous cyclonic circulation in the atmosphere and the ocean strengthens the Weddell Gyre and exerts anomalously intense stresses on the ice. Thermodynamically, the warm water advected by the intensified circulation, and most importantly entrained into the mixed layer, thins the ice from below at the beginning of the freezing season, preconditioning the region for a polynya event months later. This four-month-ahead pattern enables early predictions of the polynya, and improved expedition planning and sensor deployment.
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| 8. |
- Zhou, Lu, 1993, et al.
(författare)
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Sea Ice Production in the 2016 and 2017 Maud Rise Polynyas
- 2023
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Ingår i: Journal of Geophysical Research: Oceans. - 2169-9275 .- 2169-9291. ; 128:2
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Tidskriftsartikel (refereegranskat)abstract
- Sea ice production within polynyas, an outcome of the atmosphere-ice-ocean interaction, is a major source of dense water and hence key to the global overturning circulation, but is poorly quantified over open-ocean polynyas. Using the two recent extensive open-ocean polynyas within the wider Maud Rise region of the Weddell Sea in 2016 and 2017, we here explore the sea ice energy budget and estimate their sea ice production based on satellite retrievals, in-situ hydrographic observations and the Japanese 55-year Reanalysis. We find that the oceanic heat flux amounts to 36.1 and 30.7Wm−2 within the 2016 and 2017 polynyas, respectively. Especially the 2017 open-ocean polynya produced nearly 200km3 of new sea ice, which is comparable to the production in the largest Antarctic coastal polynyas. Finally, we determine that ice production is highly correlated with and sensitive to skin temperature and wind speed, which affect the turbulent fluxes. It is also strongly sensitive to uncertainties in the sea ice concentration and 1,000hPa temperature, which all urgently need to be better monitored at high latitudes. Lastly, more process-oriented campaigns are required to further elucidate the role of open-ocean polynya on the local and global ocean circulations.
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