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Sökning: WFRF:(Li Hao 1984 )

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51.
  • Ablikim, M., et al. (författare)
  • Observation of e(+)e(-) -> eta h(c) at center-of-mass energies from 4.085 to 4.600 GeV
  • 2017
  • Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 96:1
  • Tidskriftsartikel (refereegranskat)abstract
    • We observe for the first time the process e(+) e(-) -> eta h(c) with data collected by the BESIII experiment. Significant signals are observed at the center-of-mass energy root s = 4.226 GeV, and the Born cross section is measured to be (9.5(-2.0)(+2.2)) pb. Evidence for eta h(c) is observed at root s = 4.358 GeV with a Born cross section of (10.0(-2.7)(+3.1)) pb, and upper limits on the production cross section at other center-of-mass energies between 4.085 and 4.600 GeV are determined.
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52.
  • Ablikim, M., et al. (författare)
  • Observation of the decay Lambda(+)(c) -> Sigma(-)pi(+)pi(+)pi(0)
  • 2017
  • Ingår i: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 772, s. 388-393
  • Tidskriftsartikel (refereegranskat)abstract
    • We report the first observation of the decay Lambda(+)(c) -> Sigma(-)pi(+)pi(+)pi(0), based on data obtained in e(+)e(-) annihilations with an integrated luminosity of 567 pb(-1) at vs = 4.6 GeV. The data were collected with the BESIII detector at the BEPCII storage rings. The absolute branching fraction B(Lambda(+)(c) -> Sigma(-)pi(+)pi(+)pi(0)) is determined to be (2.11 +/- 0.33(stat.) +/- 0.14(syst.))%. In addition, an improved measurement of B(Lambda(+)(c) -> Sigma(-)pi(+)pi(+)pi(0)) is determined as (1.81 +/- 0.17(stat.) +/- 0.09(syst.))%.
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53.
  • Ablikim, M., et al. (författare)
  • Observation of the helicity-selection-rule suppressed decay of the χc2 charmonium state
  • 2017
  • Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 96:11
  • Tidskriftsartikel (refereegranskat)abstract
    • The decays of chi(c2) -> K+K- pi(0), KSK +/-pi(-/+), and pi(+)pi(-)pi(0) are studied with the psi(3686) data samples collected with the Beijing Spectrometer ( BESIII). For the first time, the branching fractions of chi(c2) -> K*(K) over bar,chi(c2) -> a(2)(+/-)(1320)pi(-/+)/a(2)(0)(1320)pi(0), and chi(c2) -> rho(770)(+/-)pi(-/+) are measured. Here, K*(K) over bar denotes both K*K-+/-(-/+) and its isospin-conjugated process K*(0)(K) over bar (0) + c: c:, and K* denotes the resonances K*(892), K*(2)(1430), and K*(3)(1780). The observations indicate a strong violation of the helicity selection rule in chi(c2) decays into vector and pseudoscalar meson pairs. The measured branching fractions of chi(c2) -> K*(892)(K) over bar are more than ten times larger than the upper limit of chi(c2) -> rho(770)(+/-)pi(-/+), which is so far the first direct observation of a significant U-spin symmetry breaking effect in charmonium decays.
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54.
  • Ablikim, M., et al. (författare)
  • Observation of Ψ(3686) → e+e-ΧcJ and ΧcJ → e+e-J/Ψ
  • 2017
  • Ingår i: Physical Review Letters. - 0031-9007 .- 1079-7114. ; 118:22
  • Tidskriftsartikel (refereegranskat)abstract
    • Using 4.479 x 10(8) psi(3686) events collected with the BESIII detector, we search for the decays psi(3686) -> e(+)e(-)chi(cJ) and chi(cJ) -> e(+)e(-)J/psi, where J = 0, 1, 2. The decays psi(3686) -> e(+)e(-)chi(cJ) and chi(cJ) -> e(+)e(-)J/psi are observed for the first time. The measured branching fractions are B(psi(3686) -> e(+)e(-)chi(cJ)) = (11.7 +/- 2.5 +/- 1.0) x 10(-4), (8.6 +/- 0.3 +/- 0.6) x 10(-4), (6.9 +/- 0.5 +/- 0.6) x 10(-4) for J = 0, 1, 2, and B(chi(cJ) -> e(+)e(-)J/psi) = (1.51 +/- 0.30 +/- 0.13)x10(-4), (3.73 +/- 0.09 +/- 0.25)x10(-3), (2.48 +/- 0.08 +/- 0.16)x10(-3) for J = 0, 1, 2, respectively. The ratios of the branching fractions B(psi(3686) -> e(+)e(-)chi(cJ))/B(psi(3686) -> gamma chi(cJ)) and B(chi(cJ) -> e(+)e(-)J/psi)/B(chi(cJ) -> gamma J/psi) are also reported. Also, the alpha values of helicity angular distributions of the e(+)e(-) pair are determined for psi(3686) -> e(+)e(-)chi(c1,2) and chi(c1,2) -> e(+)e(-)J/psi.
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55.
  • Ablikim, M., et al. (författare)
  • Observation of ψ(3686)→n¯n and improved measurement of ψ(3686)→p¯p
  • 2018
  • Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 98:3
  • Tidskriftsartikel (refereegranskat)abstract
    • We observe the decay psi (3686) -> n (n) over bar for the first time and measure psi (3686) -> p (p) over bar with improved accuracy by using 1.07 x 10(8) psi (3686) events collected with the BESIII detector. The measured branching fractions are B(psi(3686) -> n (n) over bar) = (3.06 +/- 0.06 +/- 0.14) x 10(-4) and B(psi(3686) -> p (p) over bar) = (3.05 +/- 0.02 +/- 0.12) x 10(-4). Here, the first uncertainties are statistical, and the second ones are systematic. With the hypothesis that the polar angular distributions of the neutron and proton in the center-of-mass system obey 1 + alpha cos(2) theta, we determine the a parameters to be alpha(n (n) over bar) = 0.68 +/- 0.12 +/- 0.11 and alpha(p (p) over bar) = 1.03 +/- 0.06 +/- 0.03 for psi(3686) -> n (n) over bar and psi(3686) -> p (p) over bar, respectively.
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56.
  • Ablikim, M., et al. (författare)
  • Search for h(c) -> pi(+)pi(-) J/psi via psi(3686) -> pi(0)pi(+)pi(-) J/psi
  • 2018
  • Ingår i: Physical Review D. - : American Physical Society. - 2470-0010 .- 2470-0029. ; 97:5
  • Tidskriftsartikel (refereegranskat)abstract
    • Using a data sample of 448.1 x 10(6) psi(3686) events collected with the BESIII detector operating at the BEPCII, we perform search for the hadronic transition h(c)-> pi(+)pi(-) J/psi via psi (3686) -> pi(0)hc. No signals of the transition are observed, and the upper limit on the product branching fraction B(sigma(3686) -> pi(0)h(c))B(h(c) -> pi(+)pi(-) J/psi) at the 90% confidence level (C. L.) is determined to be 2.0 x 10(-6). This is the most stringent upper limit to date.
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57.
  • Ablikim, M., et al. (författare)
  • Study of eta(1475) and X(1835) in radiative J/psi decays to gamma phi
  • 2018
  • Ingår i: Physical Review D. - : AMER PHYSICAL SOC. - 2470-0010 .- 2470-0029. ; 97:5
  • Tidskriftsartikel (refereegranskat)abstract
    • The decay J/psi -> gamma gamma phi is studied using a sample of 1.31 x 10(9) J/psi events collected with the BESIII detector. Two structures around 1475 MeV/c(2) and 1835 MeV/c(2) are observed in the gamma phi invariant mass spectrum for the first time. With a fit on the gamma phi invariant mass, which takes into account the interference between the two structures, and a simple analysis of the angular distribution, the structure around 1475 MeV/c(2) is found to favor an assignment as the eta(1475) and the mass and width for the structure around 1835 MeV/c(2) are consistent with the X(1835). The statistical significances of the two structures are 13.5 sigma and 6.3 sigma, respectively. The results indicate that both eta(1475) and X(1835) contain a sizeable s (s) over bar component.
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58.
  • Ablikim, M., et al. (författare)
  • Study of J/psi and psi(3686) -> Sigma(1385)(0)(Sigma)over-bar(1385)(0) and Xi(0)(Xi)over-bar(0) : Study of J/ψ and ψ(3686) → (1385)0( ¯ 1385)0 and 0¯ 0
  • 2017
  • Ingår i: Physics Letters B. - : ELSEVIER SCIENCE BV. - 0370-2693 .- 1873-2445. ; 770, s. 217-225
  • Tidskriftsartikel (refereegranskat)abstract
    • We study the decays of J/psi and psi(3686) to the final states Sigma(1385)(0)(Sigma) over bar (1385)(0) and Xi(0)(Xi) over bar (0) based on a single baryon tag method using data samples of (1310.6 +/- 7.0) x 10(6) J/psi and (447.9 +/- 2.9) x 10(6) psi(3686) events collected with the BESIII detector at the BEPCII collider. The decays to Sigma(1385)(0)(Sigma) over bar (1385)(0) are observed for the first time. The measured branching fractions of J/psi and psi(3686) to Xi(0)(Xi) over bar (0) are in good agreement with, and much more precise than, the previously published results. The angular parameters for these decays are also measured for the first time. The measured angular decay parameter for J/psi -> Sigma(1385)(0)(Sigma) over bar (1385)(0), alpha = -0.64 +/- 0.03 +/- 0.10, is found to be negative, different to the other decay processes in this measurement. In addition, the "12% rule" and isospin symmetry in the decays of J/psi and psi(3686) to Xi(Xi) over bar and Sigma(1385)(Sigma) over bar (1385) are tested.
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59.
  • Ablikim, M., et al. (författare)
  • Dark photon search in the mass range between 1.5 and 3.4 GeV/c
  • 2017
  • Ingår i: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 774, s. 252-257
  • Tidskriftsartikel (refereegranskat)abstract
    • Using a data set of 2.93 fb taken at a center-of-mass energy root s = 3.773 GeV with the BESIII detector at the BEPCII collider, we perform a search for an extra U(1) gauge boson, also denoted as a dark photon. We examine the initial state radiation reactions e(+)e(-) -> e(+)e(-) gamma(ISR) and e(+)e(-) -> mu(+)mu(-) gamma(ISR) for this search, where the dark photon would appear as an enhancement in the invariant mass distribution of the leptonic pairs. We observe no obvious enhancement in the mass range between 1.5 and 3.4 GeV/c(2) and set a 90% confidence level upper limit on the mixing strength of the dark photon and the Standard Model photon. We obtain a competitive limit in the tested mass range.
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60.
  • Ablikim, M., et al. (författare)
  • Measurement of cross sections of the interactions e(+)e(-) -> phi phi omega and e(+)e(-) -> phi phi phi at center-of-mass energies from 4.008 to 4.600 GeV
  • 2017
  • Ingår i: Physics Letters B. - : Elsevier BV. - 0370-2693 .- 1873-2445. ; 774, s. 78-86
  • Tidskriftsartikel (refereegranskat)abstract
    • Using data samples collected with the BESIII detector at the BEPCII collider at six center-of-mass energies between 4.008 and 4.600 GeV, we observe the processes e(+)e(-) -> phi phi omega and e(-)e(-) -> phi phi phi. The Born cross sections are measured and the ratio of the cross sections sigma(e(+)e(-) -> phi phi omega)/sigma(e(-)e(-) -> phi phi phi) is estimated to be 1.75 +/- 0.22 +/- 0.19 averaged over six energy points, where the first uncertainty is statistical and the second is systematic. The results represent first measurements of these interactions.
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61.
  • Akiyama, Kazunori, et al. (författare)
  • The persistent shadow of the supermassive black hole of M 87: I. Observations, calibration, imaging, and analysis*
  • 2024
  • Ingår i: Astronomy and Astrophysics. - 0004-6361 .- 1432-0746. ; 681
  • Tidskriftsartikel (refereegranskat)abstract
    • In April 2019, the Event Horizon Telescope (EHT) Collaboration reported the first-ever event-horizon-scale images of a black hole, resolving the central compact radio source in the giant elliptical galaxy M 87. These images reveal a ring with a southerly brightness distribution and a diameter of ∼42 μas, consistent with the predicted size and shape of a shadow produced by the gravitationally lensed emission around a supermassive black hole. These results were obtained as part of the April 2017 EHT observation campaign, using a global very long baseline interferometric radio array operating at a wavelength of 1.3 mm. Here, we present results based on the second EHT observing campaign, taking place in April 2018 with an improved array, wider frequency coverage, and increased bandwidth. In particular, the additional baselines provided by the Greenland telescope improved the coverage of the array. Multiyear EHT observations provide independent snapshots of the horizon-scale emission, allowing us to confirm the persistence, size, and shape of the black hole shadow, and constrain the intrinsic structural variability of the accretion flow. We have confirmed the presence of an asymmetric ring structure, brighter in the southwest, with a median diameter of 43.3-3.1+1.5 μas. The diameter of the 2018 ring is remarkably consistent with the diameter obtained from the previous 2017 observations. On the other hand, the position angle of the brightness asymmetry in 2018 is shifted by about 30 relative to 2017. The perennial persistence of the ring and its diameter robustly support the interpretation that the ring is formed by lensed emission surrounding a Kerr black hole with a mass ∼6.5× 109M. The significant change in the ring brightness asymmetry implies a spin axis that is more consistent with the position angle of the large-scale jet.
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62.
  • Li, Gang, 1991, et al. (författare)
  • Bayesian genome scale modelling identifies thermal determinants of yeast metabolism
  • 2021
  • Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723 .- 2041-1723. ; 12:1
  • Tidskriftsartikel (refereegranskat)abstract
    • The molecular basis of how temperature affects cell metabolism has been a long-standing question in biology, where the main obstacles are the lack of high-quality data and methods to associate temperature effects on the function of individual proteins as well as to combine them at a systems level. Here we develop and apply a Bayesian modeling approach to resolve the temperature effects in genome scale metabolic models (GEM). The approach minimizes uncertainties in enzymatic thermal parameters and greatly improves the predictive strength of the GEMs. The resulting temperature constrained yeast GEM uncovers enzymes that limit growth at superoptimal temperatures, and squalene epoxidase (ERG1) is predicted to be the most rate limiting. By replacing this single key enzyme with an ortholog from a thermotolerant yeast strain, we obtain a thermotolerant strain that outgrows the wild type, demonstrating the critical role of sterol metabolism in yeast thermosensitivity. Therefore, apart from identifying thermal determinants of cell metabolism and enabling the design of thermotolerant strains, our Bayesian GEM approach facilitates modelling of complex biological systems in the absence of high-quality data and therefore shows promise for becoming a standard tool for genome scale modeling.
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63.
  • Qin, Ning, 1990, et al. (författare)
  • Flux regulation through glycolysis and respiration is balanced by inositol pyrophosphates in yeast
  • 2023
  • Ingår i: Cell. - : Elsevier BV. - 0092-8674 .- 1097-4172. ; 186:4, s. 748-763.e15
  • Tidskriftsartikel (refereegranskat)abstract
    • Although many prokaryotes have glycolysis alternatives, it's considered as the only energy-generating glucose catabolic pathway in eukaryotes. Here, we managed to create a hybrid-glycolysis yeast. Subsequently, we identified an inositol pyrophosphatase encoded by OCA5 that could regulate glycolysis and respiration by adjusting 5-diphosphoinositol 1,2,3,4,6-pentakisphosphate (5-InsP7) levels. 5-InsP7 levels could regulate the expression of genes involved in glycolysis and respiration, representing a global mechanism that could sense ATP levels and regulate central carbon metabolism. The hybrid-glycolysis yeast did not produce ethanol during growth under excess glucose and could produce 2.68 g/L free fatty acids, which is the highest reported production in shake flask of Saccharomyces cerevisiae. This study demonstrated the significance of hybrid-glycolysis yeast and determined Oca5 as an inositol pyrophosphatase controlling the balance between glycolysis and respiration, which may shed light on the role of inositol pyrophosphates in regulating eukaryotic metabolism.
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64.
  • Tian, Z., et al. (författare)
  • Integrating concentrating PVs in biogas upgrading
  • 2018
  • Ingår i: Energy Procedia. - : Elsevier Ltd. - 1876-6102. ; , s. 598-603, s. 598-603
  • Konferensbidrag (refereegranskat)abstract
    • Biogas produced from anaerobic digestion processes has been considered as an important alternative to natural gas and plays a key role in the emerging market for renewable energy. By removing CO2, biogas can be upgraded to vehicle fuel. Chemical absorption is one of the widely used upgrading technologies, which advantages include high purity and low loss of biomethane. However, chemical absorption usually suffers from the high consumption of thermal energy, which is required by the regeneration of the solvent. Aiming at achieving a more sustainable and efficient biomethane production, this work proposed a novel system, which integrate concentrating photovoltaic/thermal hybrid (C-PV/T) in the upgrading of biogas. Due to the ability to produce electricity and heat simultaneously and efficiently, C-PV/T can provide the demands of both the electricity and heat. By doing dynamic simulation of the energy production of C-PV/T, the technical feasibility of such a system is analyzed. Based on the design to meet the heat demand of solvent regeneration, without energy storage, the produced heat can cover 17% of the heat demand of the solvent regeneration, but 51.1% of the electricity demand; meanwhile, 140.3 MWh excess electricity can be sold for one year.
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65.
  • Yang, Wenlong, et al. (författare)
  • Performance improvement and thermomechanical analysis of a novel asymmetrical annular thermoelectric generator
  • 2024
  • Ingår i: Applied Thermal Engineering. - 1359-4311. ; 237
  • Tidskriftsartikel (refereegranskat)abstract
    • Enhancing thermoelectric performance hinges on optimizing the geometry of thermoelectric legs. In this study, we present a novel asymmetrical annular thermoelectric generator (ATEG) in which the proportions of P-type and N-type legs are meticulously balanced. We construct a one-dimensional analytical model tailored to this ATEG. Utilizing this model, we derive the relationship governing thermal-electrical impedance matching in an asymmetrical ATEG and formulate a general expression for optimizing the asymmetry coefficient. We explore the influence of various thermal boundary conditions on optimal impedance matching, ideal annular leg parameters, and the optimal asymmetry coefficient. Our findings reveal that thermal boundary conditions significantly affect the optimal load ratio. Furthermore, in comparison to traditional ATEGs, our proposed asymmetrical ATEG with the optimized structure exhibits a remarkable 16.2 % increase in output power while maintaining the same material volume. Additionally, we perform a three-dimensional numerical analysis of the asymmetrical ATEG using Comsol. Our research findings indicate that introducing the asymmetric structure leads to higher maximum thermal stress on the legs. Interestingly, the study of asymmetric thermal boundary conditions highlights that improving heat transfer between the ATEG and the cooler yields higher mechanical reliability compared to enhancing heat transfer between the ATEG and the heat source.
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66.
  • Agrawal, Sumit, et al. (författare)
  • The secondary spiral lamina and its relevance in cochlear implant surgery
  • 2018
  • Ingår i: Upsala Journal of Medical Sciences. - : TAYLOR & FRANCIS LTD. - 0300-9734 .- 2000-1967. ; 123:1, s. 9-18
  • Tidskriftsartikel (refereegranskat)abstract
    • Objective: We used synchrotron radiation phase contrast imaging (SR-PCI) to study the 3D microanatomy of the basilar membrane (BM) and its attachment to the spiral ligament (SL) (with a conceivable secondary spiral lamina [SSL] or secondary spiral plate) at the round window membrane (RWM) in the human cochlea. The conception of this complex anatomy may be essential for accomplishing structural preservation at cochlear implant surgery.Material and methods: Sixteen freshly fixed human temporal bones were used to reproduce the BM, SL, primary and secondary osseous spiral laminae (OSL), and RWM using volume-rendering software. Confocal microscopy immunohistochemistry (IHC) was performed to analyze the molecular constituents.Results: SR-PCI reproduced the soft tissues including the RWM, Reissner's membrane (RM), and the BM attachment to the lateral wall (LW) in three dimensions. A variable SR-PCI contrast enhancement was recognized in the caudal part of the SL facing the scala tympani (ST). It seemed to represent a SSL allied to the basilar crest (BC). The SSL extended along the postero-superior margin of the round window (RW) and immunohistochemically expressed type II collagen.Conclusions: Unlike in several mammalian species, the human SSL is restricted to the most basal portion of the cochlea around the RW. It anchors the BM and may influence its hydro-mechanical properties. It could also help to shield the BM from the RW. The microanatomy should be considered at cochlear implant surgery.
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67.
  • Atturo, Francesca, et al. (författare)
  • The Human Cochlear Aqueduct and Accessory Canals : a Micro-CT Analysis Using a 3D Reconstruction Paradigm
  • 2018
  • Ingår i: Otology and Neurotology. - 1531-7129 .- 1537-4505. ; 39:6, s. e429-e435
  • Tidskriftsartikel (refereegranskat)abstract
    • Objective: We sought to study the anatomic variations of the cochlear aqueduct and its accessory canals in human temporal bones using micro-CT and a 3D reconstruction paradigm. More knowledge about the anatomic variations of these structures, particularly at the basal turn of the cochlea and round window niche, may be important to better preserve residual hearing as well as the neural supply during cochlear implant surgery.Methods: An archival collection of 30 human temporal bones underwent micro-CT and 3D reconstruction. A surface enhancement paradigm was applied. The application displays reconstructed slices as a 3D object with realistic 3D visualization of scanned objects. Virtual sectioning or cropping of the petrous bone presented subsequent areas. Thereby, the bony canals could be followed from inside the basal turn of cochlea and middle ear to the jugular foramen.Results: The cochlear aqueduct was always paralleled by an accessory canal containing the inferior cochlear vein. It ran from the basal turn of the cochlea and exited laterally in the jugular foramen. In 70% of the cases, a secondary accessory canal was observed and it derived mostly from a depression or infundibulum located in the floor of the round window niche. This canal also exited in the jugular foramen. The secondary accessory canal occasionally anastomosed with the primary accessory canal suggesting that it contains a vein that drains middle ear blood to the cranial sinus.Conclusion: Micro-CT with 3D surface reconstruction paradigm offers new possibilities to study the topographic anatomy of minor details in the human inner ear. The technique creates simulated transparent castings of the labyrinth with a coinciding surface view through enhancement of contrast between boundaries. Accessory canals that drain blood from the cochlea, spiral ganglion, and middle ear could be characterized three-dimensionally.
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68.
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69.
  • Cao, Zhejian, 1991, et al. (författare)
  • Synthesis of Metal-Organic Frameworks through Enzymatically Recycled Polyethylene Terephthalate
  • 2023
  • Ingår i: ACS Sustainable Chemistry & Engineering. - 2168-0485. ; 11:43, s. 15506-15512
  • Tidskriftsartikel (refereegranskat)abstract
    • Polyethylene terephthalate (PET) as one of the most produced plastics contributes to global waste pollution. Upcycling PET into value-added products therefore is of environmental and economic interest. Terephthalic acid (TPA), the monomer of PET, is a common linker for metal-organic framework (MOF) synthesis; thus, PET-to-MOF upcycling raises much research attention. However, conventional PET-to-MOF upcycling often requires PET depolymerization with strong acids or bases and high temperatures, which can lead to environmental and energy penalties. As an alternative, PETase offers a sustainable approach to depolymerizing PET under mesophilic and mild pH conditions. Here we report UiO-66, MOF-5, and MIL-101 syntheses using enzymatically recycled TPA as linkers. The enzymatically recycled TPA demonstrated low impurity, and the obtained MOFs possessed comparable crystallinity, thermal stability, and surface area. These results reveal the feasibility of MOF synthesis by using enzymatically recycled PET.
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70.
  • Enghag, Sara, et al. (författare)
  • Incus Necrosis and Blood Supply : A Micro-CT and Synchrotron Imaging Study
  • 2019
  • Ingår i: Otology and Neurotology. - : LIPPINCOTT WILLIAMS & WILKINS. - 1531-7129 .- 1537-4505. ; 40:7, s. E713-E722
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Incus necrosis is a common complication following stapes surgery and is associated with impaired microcirculation. The objective of this study was to investigate the vascular anatomy of the human incus by using light microscopy, micro-computed tomography (micro-CT), and synchrotron phase-contrast imaging (SR-PCI) for a novel three-dimensional (3D) analysis of the middle ear, mucosal folds, major vascular pathways, and intraosseous vascular bone channels. Methods: One-hundred-and-fifty temporal bones from the Uppsala collection were analyzed under light microscopy. Twenty temporal bones underwent high-resolution micro-CT scanning, and an additional seven specimens underwent SR-PCI at the Canadian Lightsource in Saskatoon, Canada. One of these specimens was from an individual who had undergone stapes surgery. Data were processed with volume-rendering software to create 3D reconstructions using scalar opacity mapping for bone transparency, cropping, and soft tissue analyses. Results: Micro-CT and SR-PCI with 3D rendering revealed the extensive vascular plexus within the un-decalcified incus bone communicating with the exterior surface. The relationship between the vessels, lenticular process, and incudostape-dial joint were clearly observed. SR-PCI allowed for histologic-level detail while preserving the specimen and its 3D relationships. Conclusion: SR-PCI with 3D reconstructions confirmed the main vascular supply to the lenticular process along the intraosseous lenticular vessels. This is the first synchrotron analysis of a patient having undergone stapes surgery, and it suggests that incus necrosis associated with stapes surgery may be caused by a disruption of the lenticular blood flow induced by the prosthesis loop, and not by strangulation of mucosal vessels as has been previously described.
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71.
  • Helpard, Luke, et al. (författare)
  • An Approach for Individualized Cochlear Frequency Mapping Determined From 3D Synchrotron Radiation Phase-Contrast Imaging
  • 2021
  • Ingår i: IEEE Transactions on Biomedical Engineering. - : Institute of Electrical and Electronics Engineers (IEEE). - 0018-9294 .- 1558-2531. ; 68:12, s. 3602-3611
  • Tidskriftsartikel (refereegranskat)abstract
    • Objective: Cochlear implants are traditionally programmed to stimulate according to a generalized frequency map, where individual anatomic variability is not considered when selecting the centre frequency of stimulation of each implant electrode. However, high variability in cochlear size and spatial frequency distributions exist among individuals. Generalized cochlear implant frequency maps can result in large pitch perception errors and reduced hearing outcomes for cochlear implant recipients. The objective of this work was to develop an individualized frequency mapping technique for the human cochlea to allow for patient-specific cochlear implant stimulation.Methods: Ten cadaveric human cochleae were scanned using synchrotron radiation phase-contrast imaging (SR-PCI) combined with computed tomography (CT). For each cochlea, ground truth angle-frequency measurements were obtained in three-dimensions using the SR-PCI CT data. Using an approach designed to minimize perceptual error in frequency estimation, an individualized frequency function was determined to relate angular depth to frequency within the cochlea.Results: The individualized frequency mapping function significantly reduced pitch errors in comparison to the current gold standard generalized approach.Conclusion and Significance: This paper presents for the first time a cochlear frequency map which can be individualized using only the angular length of cochleae. This approach can be applied in the clinical setting and has the potential to revolutionize cochlear implant programming for patients worldwide.
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72.
  • Helpard, Luke, et al. (författare)
  • Characterization of the human helicotrema : implications for cochlear duct length and frequency mapping
  • 2020
  • Ingår i: Journal of Otolaryngology - Head & Neck Surgery. - : BMC. - 1916-0216. ; 49
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Despite significant anatomical variation amongst patients, cochlear implant frequency-mapping has traditionally followed a patient-independent approach. Basilar membrane (BM) length is required for patient-specific frequency-mapping, however cochlear duct length (CDL) measurements generally extend to the apical tip of the entire cochlea or have no clearly defined end-point. By characterizing the length between the end of the BM and the apical tip of the entire cochlea (helicotrema length), current CDL models can be corrected to obtain the appropriate BM length. Synchrotron radiation phase-contrast imaging has made this analysis possible due to the soft-tissue contrast through the entire cochlear apex.Methods: Helicotrema linear length and helicotrema angular length measurements were performed on synchrotron radiation phase-contrast imaging data of 14 cadaveric human cochleae. On a sub-set of six samples, the CDL to the apical tip of the entire cochlea (CDLTIP) and the BM length (CDLBM) were determined. Regression analysis was performed to assess the relationship between CDLTIP and CDLBM.Results: The mean helicotrema linear length and helicotrema angular length values were 1.6 +/- 0.9 mm and 67.8 +/- 37.9 degrees, respectively. Regression analysis revealed the following relationship between CDLTIP and CDLBM: CDLBM = 0.88(CDLTIP) + 3.71 (R-2 = 0.995).Conclusion: This is the first known study to characterize the length of the helicotrema in the context of CDL measurements. It was determined that the distance between the end of the BM and the tip of the entire cochlea is clinically consequential. A relationship was determined that can predict the BM length of an individual patient based on their respective CDL measured to the apical tip of the cochlea.
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73.
  • Helpard, Luke, et al. (författare)
  • Three-Dimensional Modeling and Measurement of the Human Cochlear Hook Region : Considerations for Tonotopic Mapping
  • 2021
  • Ingår i: Otology and Neurotology. - : Lippincott Williams & Wilkins. - 1531-7129 .- 1537-4505. ; 42:6, s. E658-E665
  • Tidskriftsartikel (refereegranskat)abstract
    • Hypothesis: Measuring the length of the basilar membrane (BM) in the cochlear hook region will result in improved accuracy of cochlear duct length (CDL) measurements.Background: Cochlear implant pitch mapping is generally performed in a patient independent approach, which has been shown to result in place-pitch mismatches. In order to customize cochlear implant pitch maps, accurate CDL measurements must be obtained. CDL measurements generally begin at the center of the round window (RW) and ignore the basal-most portion of the BM in the hook region. Measuring the size and morphology of the BM in the hook region can improve CDL measurements and our understanding of cochlear tonotopy.Methods: Ten cadaveric human cochleae underwent synchrotron radiation phase-contrast imaging. The length of the BM through the hook region and CDL were measured. Two different CDL measurements were obtained for each sample, with starting points at the center of the RW (CDLRW) and the basal-most tip of the BM (CDLHR). Regression analysis was performed to relate CDLRW to CDLHR. A three-dimensional polynomial model was determined to describe the average BM hook region morphology.Results: The mean CDLRW value was 33.03 ± 1.62 mm, and the mean CDLHR value was 34.68 ± 1.72 mm. The following relationship was determined between CDLRW and CDLHR: CDLHR = 1.06(CDLRW)-0.26 (R2 = 0.99).Conclusion: The length and morphology of the hook region was determined. Current measurements underestimate CDL in the hook region and can be corrected using the results herein.
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74.
  • Hjort, Klas, 1964-, et al. (författare)
  • Softer, thinner and more compliant implants
  • 2018
  • Konferensbidrag (refereegranskat)abstract
    • Tissue irritation is caused by two main reasons – chemical and mechanical. In recent years, material chemical biocompatibility has been much improved but most implants used in soft tissue still have low compliance. This is especially severe in the brain, where the tissue often has a compliance of a soft hydrogel and ordinary silicone materials like PDMS have an elastic modulus up to 1,000 times higher, i.e. like a wooden stick irritating your skin. Starting from the remaining challenges of the highly successful Cochlear Implants and recent work on stretchable electronics this review conclude on the merits with soft stretchable printed circuitboards, with components of fluids, gels, and sprinkled with a smart dust of small chips.
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75.
  • Kämpfe Nordström, Charlotta, et al. (författare)
  • A Micro-CT and Synchrotron Imaging Study of the Human Endolymphatic Duct with Special Reference to Endolymph Outflow and Meniere's Disease
  • 2020
  • Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 10:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Meniere’s disease remains enigmatic, and has no treatment with sufficient evidence. The characteristic histopathological finding is endolymphatic hydrops, suggesting either an overproduction or decreased reabsorption of endolymph in the human inner ear. This study presents the first analysis of the vascular plexus around the human endolymphatic duct using micro computed tomography and coherent synchrotron radiation with phase contrast imaging. Using a software program, data were processed by volume-rendering with scalar opacity mapping to create transparent three-dimensional reconstructions. A rich vascular plexus was discovered around the endolymphatic duct that drained into collecting channels, linked to the vestibular venous outflow system. This network is believed to make up the principal route for endolymph outflow, and its associated malfunction may result in endolymphatic hydrops and Meniere’s disease.
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76.
  • Li, Hao, 1984-, et al. (författare)
  • A Synchrotron and Micro-CT Study of the Human Endolymphatic Duct System : Is Meniere's Disease Caused by an Acute Endolymph Backflow?
  • 2021
  • Ingår i: Frontiers in Surgery. - : Frontiers Media S.A.. - 2296-875X. ; 8
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: The etiology of Meniere's disease (MD) and endolymphatic hydrops believed to underlie its symptoms remain unknown. One reason may be the exceptional complexity of the human inner ear, its vulnerability, and surrounding hard bone. The vestibular organ contains an endolymphatic duct system (EDS) bridging the different fluid reservoirs. It may be essential for monitoring hydraulic equilibrium, and a dysregulation may result in distension of the fluid spaces or endolymphatic hydrops. Material and Methods: We studied the EDS using high-resolution synchrotron phase contrast non-invasive imaging (SR-PCI), and micro-computed tomography (micro-CT). Ten fresh human temporal bones underwent SR-PCI. One bone underwent micro-CT after fixation and staining with Lugol's iodine solution (I2KI) to increase tissue resolution. Data were processed using volume-rendering software to create 3D reconstructions allowing orthogonal sectioning, cropping, and tissue segmentation. Results: Combined imaging techniques with segmentation and tissue modeling demonstrated the 3D anatomy of the human saccule, utricle, endolymphatic duct, and sac together with connecting pathways. The utricular duct (UD) and utriculo-endolymphatic valve (UEV or Bast's valve) were demonstrated three-dimensionally for the first time. The reunion duct was displayed with micro-CT. It may serve as a safety valve to maintain cochlear endolymph homeostasis under certain conditions. Discussion: The thin reunion duct seems to play a minor role in the exchange of endolymph between the cochlea and vestibule under normal conditions. The saccule wall appears highly flexible, which may explain occult hydrops occasionally preceding symptoms in MD on magnetic resonance imaging (MRI). The design of the UEV and connecting ducts suggests that there is a reciprocal exchange of fluid among the utricle, semicircular canals, and the EDS. Based on the anatomic framework and previous experimental data, we speculate that precipitous vestibular symptoms in MD arise from a sudden increase in endolymph pressure caused by an uncontrolled endolymphatic sac secretion. A rapid rise in UD pressure, mediated along the fairly wide UEV, may underlie the acute vertigo attack, refuting the rupture/K+-intoxication theory.
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77.
  • Li, Hao, 1984-, et al. (författare)
  • Aeration of the Human Prussak's Space : A 3D Synchrotron Imaging Study
  • 2021
  • Ingår i: Otology and Neurotology. - : Lippincott Williams & Wilkins. - 1531-7129 .- 1537-4505. ; 42:7, s. E894-E904
  • Tidskriftsartikel (refereegranskat)abstract
    • Objectives: Prussak's space (PS) is an intricate middle ear region which may play an essential role in the development of middle ear disease. The three-dimensional (3D) anatomy of the human PS and its drainage routes remain relatively unknown. Earlier studies have histologically analyzed PS, by micro-dissection and endoscopy. Here, we used synchrotron-radiation phase-contrast imaging (SR-PCI), 3D reconstructions, and modeling to study the framework of the human PS, including aeration pathways. It may lead to increased understanding of development of middle ear pathology.Design: Nine human temporal bone specimens underwent in-line SR-PCI at the Canadian Light Source in Saskatoon, Saskatchewan, Canada. Data were processed with volume-rendering software to create 3D reconstructions using scalar opacity mapping and segmentations to visualize its walls in fixed, undecalcified human temporal bones.Results: The PS was found to be an irregular, variably shaped chamber with different aeration systems. Three different drainage pathways were found: 1) via the posterior malleolar pouch of von Troltsch in seven of nine ears; 2) directly posterior-inferior into the mesotympanum medial to the posterior malleolar pouch in one ear; and 3) anteriorly in another. The posterior-inferior communications depended on the anatomy of the posterior malleolar fold. In one bilateral case, the aeration differed between the ears. Earlier descriptions of upper ventilation routes between the PS and the epitympanic spaces could not be substantiated.Conclusions: The 3D anatomy of the membrane folds organizing the PS in humans was demonstrated for the first time using in-line SR-PCI. The PS was always aerated into the mesotympanum, suggesting its relative independence of attic ventilation. The impact of its various drainage routes on middle ear ventilation and disease were discussed.
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78.
  • Li, Hao, 1984-, et al. (författare)
  • Effects of Lactobacillus johnsonii and Lactobacillus reuteri on gut barrier function and heat shock proteins in intestinal porcine epithelial cells
  • 2015
  • Ingår i: Physiological Reports. - : WILEY. - 2051-817X. ; 3:4
  • Tidskriftsartikel (refereegranskat)abstract
    • Heat shock proteins (HSPs) are a set of highly conserved proteins that can serve as intestinal gate keepers in gut homeostasis. Here, effects of a probiotic, Lactobacillus rhamnosus GG (LGG), and two novel porcine isolates, Lactobacillus johnsonii strain P47-HY and Lactobacillus reuteri strain P43-HUV, on cyto-protective HSP expression and gut barrier function, were investigated in a porcine IPEC-J2 intestinal epithelial cell line model. The IPEC-J2 cells polarized on a permeable filter exhibited villus-like cell phenotype with development of apical microvilli. Western blot analysis detected HSP expression in IPEC-J2 and revealed that L. johnsonii and L. reuteri strains were able to significantly induce HSP27, despite high basal expression in IPEC-J2, whereas LGG did not. For HSP72, only the supernatant of L. reuteri induced the expression, which was comparable to the heat shock treatment, which indicated that HSP72 expression was more stimulus specific. The protective effect of lactobacilli was further studied in IPEC-J2 under an enterotoxigenic Escherichia coli (ETEC) challenge. ETEC caused intestinal barrier destruction, as reflected by loss of cell-cell contact, reduced IPEC-J2 cell viability and transepithelial electrical resistance, and disruption of tight junction protein zonula occludens-1. In contrast, the L. reuteri treatment substantially counteracted these detrimental effects and preserved the barrier function. L. johnsonii and LGG also achieved barrier protection, partly by directly inhibiting ETEC attachment. Together, the results indicate that specific strains of Lactobacillus can enhance gut barrier function through cytoprotective HSP induction and fortify the cell protection against ETEC challenge through tight junction protein modulation and direct interaction with pathogens.
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79.
  • Li, Hao, 1984- (författare)
  • In vitro Studies of Genodermatoses Affecting Cytoskeletal Integrity and Lipid Processing in Human Epidermis : Pathogenic Mechanisms and Effects of Retinoid Therapy
  • 2012
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Autosomal dominant epidermolytic ichthyosis (EI) is a rare disease characterized by intra-epidermal blistering due to mutations in either of two keratin genes, KRT1 and KRT10, expressed by suprabasal keratinocytes. Autosomal recessive congenital ichthyosis (ARCI) is a non-blistering, hyperkeratotic disease caused by mutations in one of the following genes: ABCA12, ALOX12B, ALOXE3, TGM1, CYP4F22, NIPAL4 and SLC27A4, which are all essential for skin barrier homeostasis. ARCI and EI often respond well to treatment with retinoids, but the mechanism of action is unclear. The aim of this thesis was to increase the knowledge of pathogenic pathways in ichthyosis and to find new explanations to the effect of retinoids.In vitro studies of immortalized keratinocytes from EI patients showed an abnormal keratin aggregation after heat stress, that could be partially inhibited by pre-treatment with all-trans retinoic acid (ATRA) or retinoic acid receptor α-agonists. ATRA treatment also reduced the relative expression of mutated vs wildtype KRT10. The clearance of ATRA in human keratinocytes was found to be mediated by CYP26B1.In skin biopsies from ARCI patients, immunofluorescence analysis of 12R-LOX, eLOX-3, TGM1, ichthyin and FATP4 showed altered expression, not only of the mutated protein, but also of the other proteins. These observations are consistent with a feedback regulatory mechanism by which the loss of one protein results in an up-regulation of other proteins. Furthermore, 12R-LOX, eLOX-3 and TGM1 were intimately co-localized in stratum corneum, as were ichthyin and FATP4, suggesting that the proteins are linked to the same metabolic pathway. When treated with a CYP26 inhibitor known to raise the endogenous ATRA level of the skin, two patients with NIPAL4 mutations, initially exhibiting increased co-localization signals for 12R-LOX and eLOX-3, displayed normalized lipoxygenase expressions and showed clinical improvement.In conclusion, mechanisms are proposed by which pathogenic keratin aggregations in EI and epidermal protein deficiencies in ARCI patients may be mitigated by retinoids. Furthermore, the vivid crosstalk between proteins incriminated in ARCI suggests that these enzymes operate along a common metabolic pathway essential for producing barrier lipids in stratum corneum. Any abrogation of this production may cause barrier failure, hence resulting in a compensatory hyperkeratosis characteristic of congenital ichthyosis.
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80.
  • Li, Hao, 1984-, et al. (författare)
  • Interactions between FATP4 and ichthyin in epidermal lipid processing may provide clues to the pathogenesis of autosomal recessive congenital ichthyosis
  • 2013
  • Ingår i: Journal of dermatological science (Amsterdam). - : Elsevier BV. - 0923-1811 .- 1873-569X. ; 69:3, s. 195-201
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Autosomal recessive congenital ichthyosis (ARCI) is caused by mutations in 10 differentgenes, of which transglutaminase-1 (TGM1) predominates. A rare form is ichthyosis prematuritysyndrome (IPS) caused by mutations in SLC27A4 encoding fatty acid transporter protein 4 (FATP4),believed to be an acyl-CoA synthetase activating long- and very-long-chain FA. Another ARCI is caused bymutations in NIPAL4, coding for ichthyin, which is proposed to be a magnesium transporter or a transmembrane receptor. A possible interaction between FATP4 and ichthyin has not been studied before.Objective:To find common denominators in the pathogenesis of ARCI.Methods:FATP4 and ichthyin were analyzed by immunofluorescence and proximity ligation assay (PLA) in healthy and ARCI patient skin and in in vitro models of ARCI epidermis.Results:Both proteins were expressed in the upper stratum granulosum of normal epidermis and PLA confirmed a close interaction between FATP4 and ichthyin. In IPS skin lacking FATP4 we found reduced ichthyin expression and this finding could be reproduced in organotypic epidermis with siRNA silenced SLC27A4. In contrast, increased FATP4 staining was found in patients with ichthyin (NIPAL4) mutations and in organotypic epidermis with silenced NIPAL4. In patients with TGM1 mutations, the expression of both FATP4 and ichthyin was increased, but the PLA signal was low probably indicating a malfunctioning protein interaction.Conclusion:Our study suggests that FATP4, ichthyin and TGM1 interact in lipid processing essential for maintaining the epidermal barrier function. It is also hypothesized that ichthyin serves as Mg2+-transporter for FATP4 in this process.
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81.
  • Li, Hao, 1984-, et al. (författare)
  • Retinoids Reduce Formation of Keratin Aggregates in Heat-stressed Immortalized Keratinocytes from an Epidermolytic Ichthyosis Patient with a KRT10 Mutation
  • 2013
  • Ingår i: Acta Dermato-Venereologica. - : Medical Journals Sweden AB. - 0001-5555 .- 1651-2057. ; 93:1, s. 44-49
  • Tidskriftsartikel (refereegranskat)abstract
    • Epidermolytic ichthyosis (EI) is an autosomal dominant epidermal skin fragility disorder caused by mutations in keratin 1 and 10 (K1 and K10) genes. Mutated keratins form characteristic aggregates in vivo and in vitro. Some patients benefit from retinoid therapy, although the mechanism is not fully understood. Our aim was to demonstrate whether retinoids affect the formation of keratin aggregates in immortalized EI cells in vitro. EI keratinocytes were seeded on cover slips, pre-treated or not with retinoids, heat-stressed, and keratin aggregate formation monitored. K10 aggregates were detected in 5% of cells in the resting state, whereas heat stress increased this proportion to 25%. When cells were pre-incubated with all-trans-retinoic acid (ATRA) or retinoic acid receptor (RAR)-α agonists the aggregates decreased in a dose-dependent manner. Furthermore, ATRA decreased the KRT10 transcripts 200-fold as well as diminished the ratio of mutant to wild-type transcripts from 0.41 to 0.35, thus providing a plausible rational for retinoid therapy of EI due to K10 mutations.
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82.
  • Li, Hao, 1984-, et al. (författare)
  • Synchrotron Radiation-Based Reconstruction of the Human Spiral Ganglion : Implications for Cochlear Implantation
  • 2020
  • Ingår i: Ear and Hearing. - : LIPPINCOTT WILLIAMS & WILKINS. - 0196-0202 .- 1538-4667. ; 41:1, s. 173-181
  • Tidskriftsartikel (refereegranskat)abstract
    • Objective: To three-dimensionally reconstruct Rosenthal's canal (RC) housing the human spiral ganglion (SG) using synchrotron radiation phase-contrast imaging (SR-PCI). Straight cochlear implant electrode arrays were inserted to better comprehend the electro-cochlear interface in cochlear implantation (CI).Design: SR-PCI was used to reconstruct the human cochlea with and without cadaveric CI. Twenty-eight cochleae were volume rendered, of which 12 underwent cadaveric CI with a straight electrode via the round window (RW). Data were input into the 3D Slicer software program and anatomical structures were modeled using a threshold paint tool.Results: The human RC and SG were reproduced three-dimensionally with artefact-free imaging of electrode arrays. The anatomy of the SG and its relationship to the sensory organ (Corti) and soft and bony structures were assessed.Conclusions: SR-PCI and computer-based three-dimensional reconstructions demonstrated the relationships among implanted electrodes, angular insertion depths, and the SG for the first time in intact, unstained, and nondecalcified specimens. This information can be used to assess stimulation strategies and future electrode designs, as well as create place-frequency maps of the SG for optimal stimulation strategies of the human auditory nerve in CI.
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83.
  • Li, Hao, 1984-, et al. (författare)
  • The expression of epidermal lipoxygenases and transglutaminase-1 is perturbed by NIPAL4 mutations : indications of a common metabolic pathway essential for skin barrier homeostasis
  • 2012
  • Ingår i: Journal of Investigative Dermatology. - : Elsevier BV. - 0022-202X .- 1523-1747. ; 132:10, s. 2368-2375
  • Tidskriftsartikel (refereegranskat)abstract
    • Autosomal recessive congenital ichthyosis (ARCI) is a heterogeneous group of skin barrier diseases due inter alia to mutations in transglutaminase-1 (TGM1), in lipoxygenases (LOXs) of the hepoxilin pathway, and in ichthyin, a putative Mg2+ transporter encoded by the NIPAL4 gene. In search of a common pathogenic pathway for ARCI, we investigated the epidermal expression of TGM1, 12R-LOX, eLOX-3, and ichthyin in skin biopsies from four healthy controls and nine patients with ARCI. In healthy skin, TGM1, ichthyin, and the LOX enzymes were predominantly expressed in the upper epidermis where colocalization signals could also be demonstrated by in situ proximity ligation assay. In patients with ALOX12B mutations and abnormal 12R-LOX expression, the colocalization signal for eLOX-3 and TGM1 was increased 4-fold. In contrast, patients with NIPAL4 mutations and abnormal ichthyin expression showed increased 12R-LOX and eLOX-3 staining and a colocalization signal of these LOXs that was three times the normal intensity. Treatment of these patients with a retinoid-mimetic drug, liarozole, normalized the expression of 12R-LOX and attenuated the colocalization signal. Altogether, our data indicate that ichthyin and TGM1 are functionally closely related in the lipid processing and that this metabolic pathway can be modified by retinoids.
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84.
  • Li, Hao, 1984-, et al. (författare)
  • The ultrastructure of a stria vascularis in the auditory organ of the cuban crocodile (Crocodylus rhombifer)
  • 2023
  • Ingår i: Frontiers in Cell and Developmental Biology. - : Frontiers Media S.A.. - 2296-634X. ; 11
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: An endocochlear potential (EP) exists in the mammalian cochlea generated by the stria vascularis and an associated fibrocyte network. It plays an essential role for sensory cell function and hearing sensitivity. In non-mammalian ectothermic animals the endocochlear potential is low and its origin somewhat unclear. In this study, we explored the crocodilian auditory organ and describe the fine structure of a stria vascularis epithelium that has not been verified in birds.Material and Methods: Three Cuban crocodiles (Crocodylus rhombifer) were analyzed with light and transmission electron microscopy. The ears were fixed in glutaraldehyde The temporal bones were drilled out and decalcified. The ears were dehydrated, and embedded and was followed by semi-thin and thin sectioning.Results: The fine structure of the crocodile auditory organ including the papilla basilaris and endolymph system was outlined. The upper roof of the endolymph compartment was specialized into a Reissner membrane and tegmentum vasculosum. At the lateral limbus an organized, multilayered, vascularized epithelium or stria vascularis was identified.Discussion: Electron microscopy demonstrates that the auditory organ in Crocodylus rhombifer, unlike in birds, contains a stria vascularis epithelium separate from the tegmentum vasculosum. It is believed to secrete endolymph and to generate a low grade endocochlear potential. It may regulate endolymph composition and optimize hearing sensitivity alongside the tegmentum vasculosum. It could represent a parallel evolution essential for the adaptation of crocodiles to their diverse habitats.
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85.
  • Li, Hao, 1984-, et al. (författare)
  • Three-dimensional tonotopic mapping of the human cochlea based on synchrotron radiation phase-contrast imaging
  • 2021
  • Ingår i: Scientific Reports. - : Springer Nature. - 2045-2322. ; 11:1
  • Tidskriftsartikel (refereegranskat)abstract
    • The human cochlea transforms sound waves into electrical signals in the acoustic nerve fibers with high acuity. This transformation occurs via vibrating anisotropic membranes (basilar and tectorial membranes) and frequency-specific hair cell receptors. Frequency-positions can be mapped within the cochlea to create a tonotopic chart which fits an almost-exponential function with lowest frequencies positioned apically and highest frequencies positioned at the cochlear base (Bekesy 1960, Greenwood 1961). To date, models of frequency positions have been based on a two-dimensional analysis with inaccurate representations of the cochlear hook region. In the present study, the first three-dimensional frequency analysis of the cochlea using dendritic mapping to obtain accurate tonotopic maps of the human basilar membrane/organ of Corti and the spiral ganglion was performed. A novel imaging technique, synchrotron radiation phase-contrast imaging, was used and a spiral ganglion frequency function was estimated by nonlinear least squares fitting a Greenwood-like function (F=A (10(ax) - K)) to the data. The three-dimensional tonotopic data presented herein has large implications for validating electrode position and creating customized frequency maps for cochlear implant recipients.
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86.
  • Li, Hao, 1984-, et al. (författare)
  • Unlocking the human inner ear for therapeutic intervention
  • 2022
  • Ingår i: Scientific Reports. - : Springer Nature. - 2045-2322. ; 12:1
  • Tidskriftsartikel (refereegranskat)abstract
    • The human inner ear contains minute three-dimensional neurosensory structures that are deeply embedded within the skull base, rendering them relatively inaccessible to regenerative therapies for hearing loss. Here we provide a detailed characterisation of the functional architecture of the space that hosts the cell bodies of the auditory nerve to make them safely accessible for the first time for therapeutic intervention. We used synchrotron phase-contrast imaging which offers the required microscopic soft-tissue contrast definition while simultaneously displaying precise bony anatomic detail. Using volume-rendering software we constructed highly accurate 3-dimensional representations of the inner ear. The cell bodies are arranged in a bony helical canal that spirals from the base of the cochlea to its apex; the canal volume is 1.6 mu L but with a diffusion potential of 15 mu L. Modelling data from 10 temporal bones enabled definition of a safe trajectory for therapeutic access while preserving the cochlea's internal architecture. We validated the approach through surgical simulation, anatomical dissection and micro-radiographic analysis. These findings will facilitate future clinical trials of novel therapeutic interventions to restore hearing.
  •  
87.
  • Li, Hao, 1984-, et al. (författare)
  • Vestibular Organ and Cochlear Implantation-A Synchrotron and Micro-CT Study
  • 2021
  • Ingår i: Frontiers in Neurology. - : Frontiers Media S.A.. - 1664-2295. ; 12
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Reports vary on the incidence of vestibular dysfunction and dizziness in patients following cochlear implantation (CI). Disequilibrium may be caused by surgery at the cochlear base, leading to functional disturbances of the vestibular receptors and endolymphatic duct system (EDS) which are located nearby. Here, we analyzed the three-dimensional (3D) anatomy of this region, aiming to optimize surgical approaches to limit damage to the vestibular organ.Material and Methods: A total of 22 fresh-frozen human temporal bones underwent synchrotron radiation phase-contrast imaging (SR-PCI). One temporal bone underwent micro-computed tomography (micro-CT) after fixation and staining with Lugol's iodine solution (I2KI) to increase tissue contrast. We used volume-rendering software to create 3D reconstructions and tissue segmentation that allowed precise assessment of anatomical relationships and topography. Macerated human ears belonging to the Uppsala collection were also used. Drilling and insertion of CI electrodes was performed with metric analyses of different trajectories.Results and Conclusions: SR-PCI and micro-CT imaging demonstrated the complex 3D anatomy of the basal region of the human cochlea, vestibular apparatus, and EDS. Drilling of a cochleostomy may disturb vestibular organ function by injuring the endolymphatic space and disrupting fluid barriers. The saccule is at particular risk due to its proximity to the surgical area and may explain immediate and long-term post-operative vertigo. Round window insertion may be less traumatic to the inner ear, however it may affect the vestibular receptors.
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88.
  • Liu, Wei, et al. (författare)
  • Immuno-surveillance and protection of the human cochlea
  • 2024
  • Ingår i: Frontiers in Neurology. - : Frontiers Media S.A.. - 1664-2295. ; 15
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Despite its location near infection-prone areas, the human inner ear demonstrates remarkable resilience. This suggests that there are inherent instruments deterring the invasion and spread of pathogens into the inner ear. Here, we combined high-resolution light microscopy, super-resolution immunohistochemistry (SR-SIM) and synchrotron phase contrast imaging (SR-PCI) to identify the protection and barrier systems in the various parts of the human inner ear, focusing on the lateral wall, spiral ganglion, and endolymphatic sac.Materials and methods: Light microscopy was conducted on mid-modiolar, semi-thin sections, after direct glutaraldehyde/osmium tetroxide fixation. The tonotopic locations were estimated using SR-PCI and 3D reconstruction in cadaveric specimens. The sections were analyzed for leucocyte and macrophage activity, and the results were correlated with immunohistochemistry using confocal microscopy and SR-SIM.Results: Light microscopy revealed unprecedented preservation of cell anatomy and several macrophage-like cells that were localized in the cochlea. Immunohistochemistry demonstrated IBA1 cells frequently co-expressing MHC II in the spiral ganglion, nerve fibers, lateral wall, spiral limbus, and tympanic covering layer at all cochlear turns as well as in the endolymphatic sac. RNAscope assays revealed extensive expression of fractalkine gene transcripts in type I spiral ganglion cells. CD4 and CD8 cells occasionally surrounded blood vessels in the modiolus and lateral wall. TMEM119 and P2Y12 were not expressed, indicating that the cells labeled with IBA1 were not microglia. The round window niche, compact basilar membrane, and secondary spiral lamina may form protective shields in the cochlear base.Discussion: The results suggest that the human cochlea is surveilled by dwelling and circulating immune cells. Resident and blood-borne macrophages may initiate protective immune responses via chemokine signaling in the lateral wall, spiral lamina, and spiral ganglion at different frequency locations. Synchrotron imaging revealed intriguing protective barriers in the base of the cochlea. The role of the endolymphatic sac in human inner ear innate and adaptive immunity is discussed.
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89.
  • Liu, Wei, et al. (författare)
  • Molecular composition and distribution of gap junctions in the sensory epithelium of the human cochlea a super-resolution structured illumination microscopy (SR-SIM) study
  • 2017
  • Ingår i: Upsala Journal of Medical Sciences. - : Uppsala Medical Society. - 0300-9734 .- 2000-1967. ; 122:3, s. 160-170
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Mutations in the GJB2 gene, which encodes the Connexin26 (Cx26) protein, are the most common cause of childhood hearing loss in American and European populations. The cochlea contains a gap junction (GJ) network in the sensory epithelium and two connective tissue networks in the lateral wall and spiral limbus. The syncytia contain the GJ proteins beta 2 (GJB2/Cx26) and beta 6 (GJB6/Cx30). Our knowledge of their expression in humans is insufficient due to the limited availability of tissue. Here, we sought to establish the molecular arrangement of GJs in the epithelial network of the human cochlea using surgically obtained samples. Methods: We analyzed Cx26 and Cx30 expression in GJ networks in well-preserved adult human auditory sensory epithelium using confocal, electron, and super -resolution structured illumination microscopy (SR-SIM). Results: Cx30 plaques (<5 mu m) dominated, while Cx26 plaques were subtle and appeared as 'mini junctions' (2-300 nm). 3-D volume rendering of Z-stacks and orthogonal projections from single optical sections suggested that the GJs are homomeric/homotypic and consist of assemblies of identical GJs composed of either Cx26 or Cx30. Occasionally, the two protein types were co-expressed, suggesting functional cooperation. Conclusions: Establishing the molecular composition and distribution of the GJ networks in the human cochlea may increase our understanding of the pathophysiology of Cx-related hearing loss. This information may also assist in developing future strategies to treat genetic hearing loss.
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90.
  • Liu, Wei, et al. (författare)
  • Spike Generators and Cell Signaling in the Human Auditory Nerve : An Ultrastructural, Super-Resolution, and Gene Hybridization Study
  • 2021
  • Ingår i: Frontiers in Cellular Neuroscience. - : Frontiers Media S.A.. - 1662-5102. ; 15
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: The human auditory nerve contains 30,000 nerve fibers (NFs) that relay complex speech information to the brain with spectacular acuity. How speech is coded and influenced by various conditions is not known. It is also uncertain whether human nerve signaling involves exclusive proteins and gene manifestations compared with that of other species. Such information is difficult to determine due to the vulnerable, "esoteric," and encapsulated human ear surrounded by the hardest bone in the body. We collected human inner ear material for nanoscale visualization combining transmission electron microscopy (TEM), super-resolution structured illumination microscopy (SR-SIM), and RNA-scope analysis for the first time. Our aim was to gain information about the molecular instruments in human auditory nerve processing and deviations, and ways to perform electric modeling of prosthetic devices.Material and Methods: Human tissue was collected during trans-cochlear procedures to remove petro-clival meningioma after ethical permission. Cochlear neurons were processed for electron microscopy, confocal microscopy (CM), SR-SIM, and high-sensitive in situ hybridization for labeling single mRNA transcripts to detect ion channel and transporter proteins associated with nerve signal initiation and conductance.Results: Transport proteins and RNA transcripts were localized at the subcellular level. Hemi-nodal proteins were identified beneath the inner hair cells (IHCs). Voltage-gated ion channels (VGICs) were expressed in the spiral ganglion (SG) and axonal initial segments (AISs). Nodes of Ranvier (NR) expressed Nav1.6 proteins, and encoding genes critical for inter-cellular coupling were disclosed.Discussion: Our results suggest that initial spike generators are located beneath the IHCs in humans. The first NRs appear at different places. Additional spike generators and transcellular communication may boost, sharpen, and synchronize afferent signals by cell clusters at different frequency bands. These instruments may be essential for the filtering of complex sounds and may be challenged by various pathological conditions.
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91.
  • Mei, Xueshuang, et al. (författare)
  • Human inner ear blood supply revisited : the Uppsala collection of temporal bone - an international resource of education and collaboration
  • 2018
  • Ingår i: Upsala Journal of Medical Sciences. - : TAYLOR & FRANCIS LTD. - 0300-9734 .- 2000-1967. ; 123:3, s. 131-142
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: The Uppsala collection of human temporal bones and molds is a unique resource for education and international research collaboration. Micro-computerized tomography (micro-CT) and synchrotron imaging are used to investigate the complex anatomy of the inner ear. Impaired microcirculation is etiologically linked to various inner ear disorders, and recent developments in inner ear surgery promote examination of the vascular system. Here, for the first time, we present three-dimensional (3D) data from investigations of the major vascular pathways and corresponding bone channels.Methods: We used the archival Uppsala collection of temporal bones and molds consisting of 324 inner ear casts and 113 macerated temporal bones. Micro-CT was used to investigate vascular bone channels, and 26 fresh human temporal bones underwent synchrotron radiation phase contrast imaging (SR-PCI). Data were processed by volume-rendering software to create 3D reconstructions allowing orthogonal sectioning, cropping, and soft tissue analyses.Results: Micro-CT with 3D rendering was superior in reproducing the anatomy of the vascular bone channels, while SR-PCI replicated soft tissues. Arterial bone channels were traced from scala vestibuli (SV) arterioles to the fundus, cochlea, and vestibular apparatus. Drainage routes along the aqueducts were examined.Conclusion: Human inner ear vessels are difficult to study due to the adjoining hard bone. Micro-CT and SR-PCI with 3D reconstructions revealed large portions of the micro-vascular system in un-decalcified specimens. The results increase our understanding of the organization of the vascular system in humans and how altered microcirculation may relate to inner ear disorders. The findings may also have surgical implications.
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92.
  • Mei, Xueshuang, et al. (författare)
  • Three-dimensional imaging of the human internal acoustic canal and arachnoid cistern : a synchrotron study with clinical implications
  • 2019
  • Ingår i: Journal of Anatomy. - : WILEY. - 0021-8782 .- 1469-7580. ; 234:3, s. 316-326
  • Tidskriftsartikel (refereegranskat)abstract
    • A thorough knowledge of the gross and micro-anatomy of the human internal acoustic canal (IAC) is essential in vestibular schwannoma removal, cochlear implantation (CI) surgery, vestibular nerve section, and decompression procedures. Here, we analyzed the acoustic-facial cistern of the human IAC, including nerves and anastomoses using synchrotron phase contrast imaging (SR-PCI). A total of 26 fresh human temporal bones underwent SR-PCI. Data were processed using volume-rendering software to create three-dimensional (3D) reconstructions allowing soft tissue analyses, orthogonal sectioning, and cropping. A scalar opacity mapping tool was used to enhance tissue surface borders, and anatomical structures were color-labeled for improved 3D comprehension of the soft tissues. SR-PCI reproduced, for the first time, the variable 3D anatomy of the human IAC, including cranial nerve complexes, anastomoses, and arachnoid membrane invagination (acoustic-facial cistern; an extension of the cerebellopontine cistern) in unprocessed, un-decalcified specimens. An unrecognized system of arachnoid pillars and trabeculae was found to extend between the arachnoid and cranial nerves. We confirmed earlier findings that intra-meatal vestibular schwannoma may grow unseparated from adjacent nerves without duplication of the arachnoid layers. The arachnoid pillars may support and stabilize cranial nerves in the IAC and could also play a role in local fluid hydrodynamics.
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93.
  • Mei, Xueshuang, et al. (författare)
  • Vascular Supply of the Human Spiral Ganglion : Novel Three-Dimensional Analysis Using Synchrotron Phase-Contrast Imaging and Histology
  • 2020
  • Ingår i: Scientific Reports. - : Springer Nature. - 2045-2322. ; 10:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Human spiral ganglion (HSG) cell bodies located in the bony cochlea depend on a rich vascular supply to maintain excitability. These neurons are targeted by cochlear implantation (CI) to treat deafness, and their viability is critical to ensure successful clinical outcomes. The blood supply of the HSG is difficult to study due to its helical structure and encasement in hard bone. The objective of this study was to present the first three-dimensional (3D) reconstruction and analysis of the HSG blood supply using synchrotron radiation phase-contrast imaging (SR-PCI) in combination with histological analyses of archival human cochlear sections. Twenty-six human temporal bones underwent SR-PCI. Data were processed using volume-rendering software, and a representative three-dimensional (3D) model was created to allow visualization of the vascular anatomy. Histologic analysis was used to verify the segmentations. Results revealed that the HSG is supplied by radial vascular twigs which are separate from the rest of the inner ear and encased in bone. Unlike with most organs, the arteries and veins in the human cochlea do not follow the same conduits. There is a dual venous outflow and a modiolar arterial supply. This organization may explain why the HSG may endure even in cases of advanced cochlear pathology.
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94.
  • Rask-Andersen, Helge, et al. (författare)
  • Special Anatomic Considerations in Otosclerosis Surgery
  • 2018
  • Ingår i: Otolaryngologic clinics of North America. - : Saunders Elsevier. - 0030-6665 .- 1557-8259. ; 51:2, s. 357-
  • Tidskriftsartikel (refereegranskat)abstract
    • The anatomy of the vestibular organs together with considerations of the middle and inner ear anatomy relevant to stapes surgery is discussed. An archival collection of macerated and freshly frozen human temporal bones underwent micro computed tomography (CT) with subsequent volume rendering. Three-dimensional (3D) reconstructions and the topographic anatomy of the oval window were considered. Micro-CT and 3D rendering revealed the relationship between the otolith organs and the oval window. Anatomic variations were extensive and included the distance between the footplate and the reconstructed macula margins. A "no-go" zone is suggested for the surgeon to avoid injury during stapes surgery.
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95.
  • Schart-Moren, Nadine, et al. (författare)
  • Anatomical Characteristics of Facial Nerve and Cochlea Interaction
  • 2017
  • Ingår i: Audiology & neuro-otology. - : KARGER. - 1420-3030 .- 1421-9700. ; 22:1, s. 41-49
  • Tidskriftsartikel (refereegranskat)abstract
    • Objective: The aim was to study the relationship between the labyrinthine portion (LP) of the facial canal and the cochlea in human inner ear molds and temporal bones using micro-CT and 3D rendering. A reduced cochlea-facial distance may spread electric currents from the cochlear implant to the LP and cause facial nerve stimulation. Influencing factors may be the topographic anatomy and otic capsule properties.Methods: An archival collection of human temporal bones underwent micro-CT and 3D reconstruction. In addition, cochlea-facial distance was assessed in silicone and polyester resin molds, and the association between the LP and upper basal turn of the cochlea was analyzed.Results: Local thinning of the otic capsule and local anatomy may explain the development of cochlea-facial dehiscence, which was found in 1.4%. A reduced cochlea-facial distance was noted in 1 bone with a superior semicircular canal dehiscence but not in bones with superior semicircular canal "blue line." The otic capsule often impinged upon the LP and caused narrowing.Conclusion: Micro-CT with 3D rendering offers new possibilities to study the topographic anatomy of the human temporal bone. The varied shape of the cross-section of the LP could often be explained by an "intruding" cochlea.
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96.
  • Schart-Moren, Nadine, et al. (författare)
  • Cochlear implantation and residual hearing preservation long-term follow-up of the first consecutively operated patients using the round window approach in Uppsala, Sweden
  • 2020
  • Ingår i: Cochlear Implants International. - : Taylor & Francis Group. - 1467-0100 .- 1754-7628. ; 21:5, s. 246-259
  • Tidskriftsartikel (refereegranskat)abstract
    • Objective: We conducted a long-term follow-up study to investigate the time course of residual hearing in our first 21 consecutively operated cochlear implant (CI) patients using the round window (RW) approach . The study may provide additional information about the influence of a flexible lateral wall electrode array on cochlear function.Methods: Data were available for long-term follow-up (>5 years) in 15 patients. Pure tone audiometry (PTA) was assessed at 0.125–8 kHz preoperatively, and at one, three and >5 years postoperatively. Insertion angle, number of electrodes inside the cochlea, user-time of the processor and stimulation strategy were documented.Results: Twelve out of 15 patients had residual hearing after a follow-up period of five years (mean 86 months, range: 61–103 months). Four out of 15 patients had >75% complete hearing preservation (HP), 8 out of 15 had 25–75% partial HP and 3 out of 15 patients had complete loss of hearing. There was a high correlation between insertion angle and HP.Conclusion: Long-term HP was possible in 12 out of 15 cases. Even patients with complete hearing loss at long-term follow-up showed high performance in speech understanding and were full-time users.
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97.
  • Schart-Moren, Nadine, et al. (författare)
  • Effects of Various Trajectories on Tissue Preservation in Cochlear Implant Surgery : A Micro-Computed Tomography and Synchrotron Radiation Phase-Contrast Imaging Study
  • 2019
  • Ingår i: Ear and Hearing. - 0196-0202 .- 1538-4667. ; 40:2, s. 393-400
  • Tidskriftsartikel (refereegranskat)abstract
    • OBJECTIVES:The purpose of this study was to evaluate the three-dimensional (3D) anatomy and potential damage to the hook region of the human cochlea following various trajectories at cochlear implantation (CI). The goal was to determine which of the approaches can avoid lesions to the soft tissues, including the basilar membrane and its suspension to the lateral wall. Currently, there is increased emphasis on conservation of inner ear structures, even in nonhearing preservation CI surgery.DESIGN:Micro-computed tomography and various CI approaches were made in an archival collection of macerated and freshly fixed human temporal bones. Furthermore, synchrotron radiation phase-contrast imaging was used to reproduce the soft tissues. The 3D anatomy was investigated using bony and soft tissue algorithms, and influences on inner ear structures were examined.RESULTS:Micro-computed tomography with 3D rendering demonstrated the topography of the round window (RW) and osseous spiral laminae, while synchrotron imaging allowed reproduction of soft tissues such as the basilar membrane and its suspension around the RW membrane. Anterior cochleostomies and anteroinferior cochleostomies invariably damaged the intracochlear soft tissues while inferior cochleostomies sporadically left inner ear structures unaffected.CONCLUSIONS:Results suggest that cochleostomy approaches often traumatize the soft tissues at the hook region at CI surgery. For optimal structural preservation, the RW approach is, therefore, recommended.
  •  
98.
  • Schart-Moren, Nadine, et al. (författare)
  • Three-Dimensional Analysis of the Fundus of the Human Internal Acoustic Canal
  • 2018
  • Ingår i: Ear and Hearing. - : Lippincott Williams & Wilkins. - 0196-0202 .- 1538-4667. ; 39:3, s. 563-572
  • Tidskriftsartikel (refereegranskat)abstract
    • Objectives: Documentation of the nerve components in the internal acoustic canal is essential before cochlea implantation surgery. Interpretations may be challenged by wide anatomical variations of the VIIIth nerve and their ramifications. Malformations may further defy proper nerve identification. Design: Using microcomputed tomography, we analyzed the fundus bone channels in an archival collection of 113 macerated human temporal bones and 325 plastic inner molds. Data were subsequently processed by volume-rendering software using a bony tissue algorithm. Three-dimensional reconstructions were made, and through orthogonal sections, the topographic anatomy was established. Results: The technique provided additional information regarding the anatomy of the nerve foramina/channels of the human fundus region, including variations and destinations. Channel anastomosis were found beyond the level of the fundus. A foramen of the transverse crest was identified. Conclusions: Three-dimensional reconstructions and cropping outlined the bone canals and demonstrated the highly variable VIIIth nerve anatomy at the fundus of the human inner acoustic canal. Myriad channel interconnections suggested an intricate system of neural interactive pathways in humans. Particularly striking was the variable anatomy of the saccule nerve channels. The results may assist in the preoperative interpretation of the VIIIth nerve anatomy.
  •  
99.
  • Senn, Pascal, et al. (författare)
  • NANOCI-Nanotechnology Based Cochlear Implant With Gapless Interface to Auditory Neurons
  • 2017
  • Ingår i: Otology and Neurotology. - 1531-7129 .- 1537-4505. ; 38:8, s. E224-E231
  • Tidskriftsartikel (refereegranskat)abstract
    • Cochlear implants (CI) restore functional hearing in the majority of deaf patients. Despite the tremendous success of these devices, some limitations remain. The bottleneck for optimal electrical stimulation with CI is caused by the anatomical gap between the electrode array and the auditory neurons in the inner ear. As a consequence, current devices are limited through 1) low frequency resolution, hence suboptimal sound quality and 2), large stimulation currents, hence high energy consumption (responsible for significant battery costs and for impeding the development of fully implantable systems). A recently completed, multinational and interdisciplinary project called NANOCI aimed at overcoming current limitations by creating a gapless interface between auditory nerve fibers and the cochlear implant electrode array. This ambitious goal was achieved in vivo by neurotrophin-induced attraction of neurites through an intra-cochlear gel-nanomatrix onto a modified nanoCI electrode array located in the scala tympani of deafened guinea pigs. Functionally, the gapless interface led to lower stimulation thresholds and a larger dynamic range in vivo, and to reduced stimulation energy requirement (up to fivefold) in an in vitro model using auditory neurons cultured on multi-electrode arrays. In conclusion, the NANOCI project yielded proof of concept that a gapless interface between auditory neurons and cochlear implant electrode arrays is feasible. These findings may be of relevance for the development of future CI systems with better sound quality and performance and lower energy consumption. The present overview/review paper summarizes the NANOCI project history and highlights achievements of the individual work packages.
  •  
100.
  • Shao, Xue-Feng, et al. (författare)
  • Hydroxyl group functionalized graphene oxide nanosheets as additive for improved erythritol latent heat storage performance : A comprehensive evaluation on the benefits and challenges
  • 2020
  • Ingår i: Solar Energy Materials and Solar Cells. - : Elsevier BV. - 0927-0248 .- 1879-3398. ; 215
  • Tidskriftsartikel (refereegranskat)abstract
    • Graphene oxide (GO) nanosheets were employed as the additive to make composites of erythritol, a promising medium-temperature PCM candidate. GO nanosheets modified with hydroxyl groups were applied to improve the dispersion stability of the composites. A systematic characterization on the latent heat storage performance was performed for both pure and composite erythritol, in order to identify the benefits and challenges of the composites. It was found that the thermal conductivity is increased by nearly twice and the degree of supercooling was lowered from ~64 °C to ~48 °C at the loading of 1.0 wt% GO nanosheets (the maximum loading tested). The addition of GO nanosheets also leads to an increase of the retrievable latent heat during crystallization, from ~187 kJ/kg to ~225 kJ/kg at the same loading, by increasing the crystallinity. However, the introduction of GO nanosheets can also lead to a rise in the dynamic viscosity of erythritol. As a result, the crystallization rate is slowed down and accordingly, the duration of crystallization becomes 62% longer when the loading reaches 1.0 wt%. In addition, favorable dispersion stability of the erythritol composites is observed, and their melting point (~117 °C) remains almost unchanged during 50 melting-crystallization cycles. Functionalized GO nanosheets have been shown to be an efficient additive for improving the performance of erythritol, but a trade-off analysis on the loading would be required to achieve the best overall performance.
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