| 51. |
- Dastanpour, E., et al.
(författare)
-
Crystallization behavior, soft magnetism and nanoindentation of Fe–Si–B–P–Cu alloy on Ni substitution
- 2021
-
Ingår i: Journal of Alloys and Compounds. - : Elsevier BV. - 0925-8388 .- 1873-4669. ; 851
-
Tidskriftsartikel (refereegranskat)abstract
- The present work investigates how the substitution of Ni for Fe in the amorphous precursor of the high flux density Fe–Si–B–P–Cu (Nanomet®) alloy avoids the creation of detrimental pre-existing nuclei in the amorphous precursor as a step forward for improved amorphization capability, retains homogenous nanocrystalline structure with excellent soft magnetic properties, and affects the mechanical properties in terms of reduced hardness and Young's modulus. This has been achieved by adding Ni of various concentrations (0–8 atomic %). The investigation includes structural characterization, calorimetry, optimization of annealing temperature, extensive magnetic characterization and nanoindentation to assess the mechanical properties. The excellent soft magnetic properties demonstrate a strategy to deploy the nanocrystalline ribbons where freedom of device design is a limiting factor for electrodynamic energy conversion applications.
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| 52. |
- Dastanpour, E., et al.
(författare)
-
On the glass forming ability (GFA), crystallization behavior and soft magnetic properties of nanomet-substituted alloys
- 2020
-
Ingår i: Journal of Non-Crystalline Solids. - : ELSEVIER. - 0022-3093 .- 1873-4812. ; 529
-
Tidskriftsartikel (refereegranskat)abstract
- This study was aimed to investigate the effect of substitution of Fe with Co, C, and Mo on the glass forming ability (GFA), crystallization behavior, and magnetic properties of Fe-B-Si-P-Cu (Nanomet) alloy. The thermodynamic parameters, P-HS and P-HSS, were used to guide towards increased GFA. The P-HSS enhanced from -2.04 kJ/mol for Nanomet to -4.83 kJ/mol for a Co4C1Mo1 (at.%) substituted alloy. As a result, the critical quench rate reduced significantly, manifested as a drop of the required rotational speed from 3000 rpm to 2000 rpm. The temperature interval between two crystallization peaks enlarged for the substituted alloy, allowing a broader annealing range for nanocrystallization. The saturation magnetization (M-S) and the coercivity (H-C) were nearly maintained. This work confirms the relevance of the parameters P-HS and P-HSS to improve the GFA of the Nanomet alloy, and it is suggested that the same strategy can be applied for other alloys.
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| 53. |
- Dastanpour, Esmat, et al.
(författare)
-
Quantification of the anomalous crystallization and soft magnetic properties of Fe-Si-B-P-Cu (Nanomet) by isothermal calorimetry
- 2020
-
Ingår i: Journal of Alloys and Compounds. - : Elsevier. - 0925-8388 .- 1873-4669. ; 830
-
Tidskriftsartikel (refereegranskat)abstract
- The present study aimed to quantify the anomalous crystallization and soft magnetic properties of Fe-Si-B-P-Cu (Nanomet) alloy by isothermal calorimetry. The isothermal crystallization had slow kinetics at temperatures below the peak temperature of the exothermic event. The inhomogeneous distribution of pre-existing nuclei in the amorphous structure led to the anomalous crystallization, and hence, to a nonlinear Avrami plot with lowered localized Avrami exponents, attributed to temperature dependent crystallization kinetics. To the best of our knowledge, the presence of pre-existing magnetic nuclei was experimentally confirmed for the first time using ultra-high sensitive magneto-thermo-gravimetry (MTG). This is otherwise challenging, if not impossible, with conventional structural diffraction techniques. The incremental saturation magnetization (Ms) revealed how the volume fraction of the nanocrystallites intrinsically depends on both the annealing temperature and dwell time, and the significant change in the coercivity (He) confirmed the vital role the homogenous nucleation growth process has in order to achieve excellent soft magnetic properties.
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| 54. |
- Dastanpour Hosseinabadi, Esmat, et al.
(författare)
-
Multi-alloying of nanomet : conception and implementation of homogeneous nanocrystallization in high-flux density soft magnetic alloys
- 2021
-
Ingår i: Journal of Materials Science. - : Springer. - 0022-2461 .- 1573-4803. ; 56:16, s. 10124-10134
-
Tidskriftsartikel (refereegranskat)abstract
- This study demonstrates how multi-alloying the Fe-Si–B–P–Cu (Nanomet®) can avoid the strict requirements on the annealing scheme in terms of high heating rate and narrow annealing temperature range in order to grow a homogeneous ultrafine nanocrystalline structure. The rather restricted amorphization capability sets a low limit of the maximum thickness of the amorphous precursor. These shortcomings have their origin in the existence of detrimental pre-existing nuclei in the amorphous precursors, which in turn potentially lead to a heterogeneous crystallization. Here, we have multialloyed Nanomet with CoCNi- and CoCMo- to avoid the creation of these pre-existing nuclei. This leads to improved amorphization capability and changes a potentially heterogeneous crystallization to a homogeneous nanocrystallization over a much broader temperature range than for unalloyed Nanomet. Thus, the requirements for the annealing are much relaxed. This work encompasses quenching the amorphous precursors using melt-spinning, investigating the crystallization temperatures by calorimetry, showing the depletion of pre-existing nuclei by magneto-thermo-gravimetry, conceptualizing the crystallization dynamics using isothermal calorimetry, and finally revealing the excellent soft magnetic properties over a broad annealing temperature interval (390–490 °C for the substituted alloys compared to 410–470 °C for unalloyed Nanomet). The multi-elemental substitution of Fe with CoCMo and CoCNi in Nanomet alloy nearly maintains the saturation magnetization and the coercivity. We believe the substituted alloys provide a better alternative to Nanomet with improved amorphization capability and homogeneous nanocrystallization without any special heat treatment scheme. Graphical abstract: [Figure not available: see fulltext.]
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| 55. |
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| 56. |
- de Oliveira, Felipe Marques Souza, et al.
(författare)
-
Detection of post-translational modifications using solid-phase proximity ligation assay.
- 2018
-
Ingår i: New biotechnology. - : Elsevier BV. - 1876-4347 .- 1871-6784. ; 45:October, s. 51-59
-
Tidskriftsartikel (refereegranskat)abstract
- Post-translational modifications (PTMs) regulate protein activities to help orchestrate and fine-tune cellular processes. Dysregulation of PTMs is often related with disorders and malignancies, and may serve as a precise biomarker of disease. Developing sensitive tools to measure and monitor low-abundant PTMs in tissue lysates or serum will be instrumental for opening up new PTM-based diagnostic avenues. Here, we investigate the use of solid-phase proximity ligation assay (SP-PLA) for detection of different PTMs. The assay depends on the recognition of the target protein molecule and its modification by three affinity binders. Using antibodies and lectins, we applied the method for detection of glycosylated CD44 and E-Cadherin, and phosphorylated p53 and EGFR. The assay was found to have superior dynamic range and limit of detection compared to standard ELISAs. In summary, we have established the use of SP-PLA as an appropriate method for sensitive detection of PTMs in lysates and sera, which may provide a basis for future PTM-based diagnostic and prognostic biomarkers.
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| 57. |
- Flowers, Sarah A., et al.
(författare)
-
Lubricin binds cartilage proteins, cartilage oligomeric matrix protein, fibronectin and collagen II at the cartilage surface
- 2017
-
Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 7
-
Tidskriftsartikel (refereegranskat)abstract
- Lubricin, a heavily O-glycosylated protein, is essential for boundary lubrication of articular cartilage. Strong surface adherence of lubricin is required given the extreme force it must withstand. Disulfide bound complexes of lubricin and cartilage oligomeric matrix protein (COMP) have recently been identified in arthritic synovial fluid suggesting they may be lost from the cartilage surface in osteoarthritis and inflammatory arthritis. This investigation was undertaken to localise COMP-lubricin complexes within cartilage and investigate if other cartilage proteins are involved in anchoring lubricin to the joint. Immunohistochemical analysis of human cartilage biopsies showed lubricin and COMP co-localise to the cartilage surface. COMP knockout mice, however, presented with a lubricin layer on the articular cartilage leading to the further investigation of additional lubricin binding mechanisms. Proximity ligation assays (PLA) on human cartilage biopsies was used to localise additional lubricin binding partners and demonstrated that lubricin bound COMP, but also fibronectin and collagen II on the cartilage surface. Fibronectin and collagen II binding to lubricin was confirmed and characterised by solid phase binding assays with recombinant lubricin fragments. Overall, COMP, fibronectin and collagen II bind lubricin, exposed on the articular cartilage surface suggesting they may be involved in maintaining essential boundary lubrication.
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| 58. |
- Georgantopoulos, Christos K., et al.
(författare)
-
Derivation of a qualitative model for the spatial characteristic wavelength of extrusion flow instabilities: Investigation of a polybutadiene rubber through capillary, slit and complex geometry extrusion dies
- 2022
-
Ingår i: Macromolecular Materials and Engineering. - : Wiley. - 1439-2054 .- 1438-7492. ; 307
-
Tidskriftsartikel (refereegranskat)abstract
- The extrusion flow instabilities of a commercial polybutadiene (PBD) are investigated as a function of different extrusion die geometries, such as round capillary, slit, and complex cross-section profile slit dies via capillary rheology. Qualitative models are used to fit the experimental data for the spatial characteristic wavelength (λ) of the appearing extrusion flow instabilities. A new qualitative model for the slit die geometry, rectangular cross-section, is derived based on the theoretical concept of the “two layers” extrudate and the force balance at the die exit region. The proposed qualitative model for the slit die geometry is used to predict the spatial characteristic wavelength (λ) for extrudates obtained by complex cross-section profile slit die geometries similar to industrial manufacturing. Correlation between the ratio of the extensional ( ) and shear ( ) stress at the die exit area and the characteristic dimension, height H for slit dies and diameter D for round capillary dies, is presented. Moreover, a geometry-dependent model is used to predict the spatial characteristic wavelength (λ) of the extrusion flow instabilities from a round capillary die to a slit die and vice versa.
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| 59. |
- Georgantopoulos, Christos K., et al.
(författare)
-
Modeling the spatial characteristics of extrusion flow instabilities for styrene-butadiene rubbers: Investigating the influence of molecular weight distribution, molecular architecture, and temperature
- 2021
-
Ingår i: Physics of Fluids. - : AIP Publishing. - 1089-7666 .- 1070-6631. ; 33:9
-
Tidskriftsartikel (refereegranskat)abstract
- The extrusion flow instabilities of three commercial styrene-butadiene rubbers (SBR) are investigated as a function of molecular weight distribution (MWD); molecular architecture (linear, branched); and temperature. The samples have multimodal MWD, with the main component being SBR and a low amount, less than 10 wt. %, of low-molecular weight hydrocarbons. Deviation from the Cox-Merz rule at high angular frequencies/shear rates becomes intense as the amount of medium-molecular weight component increases. Optical analysis is used to identify and quantify spatial surface distortions, specifically wavelength (λ) and height (h), of the different types of extrusion flow instabilities. Qualitative constitutive models are reviewed and used to fit the experimental data for the spatial characteristics of extrusion flow instability. The fitting parameters as obtained by the models are correlated with molecular properties of the materials. It is found that the characteristic spatial wavelength (λ) increases as the extrusion temperature decreases. Hence, the influence of temperature on the spatial characteristic wavelength is investigated and an Arrhenius behavior is observed.
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| 60. |
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| 61. |
- Laniel, Dominique, et al.
(författare)
-
Aromatic hexazine [N6]4− anion featured in the complex structure of the high-pressure potassium nitrogen compound K9N56
- 2023
-
Ingår i: Nature Chemistry. - : NATURE PORTFOLIO. - 1755-4330 .- 1755-4349. ; 15:5, s. 641-646
-
Tidskriftsartikel (refereegranskat)abstract
- The recent high-pressure synthesis of pentazolates and the subsequent stabilization of the aromatic [N-5](-) anion at atmospheric pressure have had an immense impact on nitrogen chemistry. Other aromatic nitrogen species have also been actively sought, including the hexaazabenzene N-6 ring. Although a variety of configurations and geometries have been proposed based on ab initio calculations, one that stands out as a likely candidate is the aromatic hexazine anion [N-6](4-). Here we present the synthesis of this species, realized in the high-pressure potassium nitrogen compound K9N56 formed at high pressures (46 and 61 GPa) and high temperature (estimated to be above 2,000 K) by direct reaction between nitrogen and KN3 in a laser-heated diamond anvil cell. The complex structure of K9N56-composed of 520 atoms per unit cell-was solved based on synchrotron single-crystal X-ray diffraction and corroborated by density functional theory calculations. The observed hexazine anion [N-6](4-) is planar and proposed to be aromatic.
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| 62. |
- Löfgren, Johan, et al.
(författare)
-
Implementation of an SVD Based MIMO OFDM channel estimator
- 2010
-
Ingår i: 2009 NORCHIP. - 9781424443109 - 9781424443116
-
Konferensbidrag (refereegranskat)abstract
- This paper presents a hardware design of an SVD based channel estimator. The details of the design are explained and some key aspects are discussed. The design has been implemented and tested on an FPGA and synthesized for an ASIC in 130 nm technology. It is shown that it is possible to get a clock frequency of 179 MHz for a 1.38 mm 2 design. This corresponds to ~30 M estimates per second, which is more than needed in current wireless systems. Further, simulations show that this design would consume an average power of around 8.5 mW with a peak power at 14.2 mW. The presented data shows that it is possible to use these kind of advanced channel estimation strategies in wireless receivers, even though there has been no prior reports of these being implemented.
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| 63. |
- Lönn, Peter, et al.
(författare)
-
High-throughput in situ mapping of phosphorylated protein complexes across the cell cycle and in response to drugs
-
Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Interactions and posttranslational modifications (PTMs) of proteins orchestrate cellular responses to cytokines, drugs or other agents, but it has been difficult to monitor and characterize these dynamic events at high-throughput. Here, we have established a semi-automated system for large-scale in situ proximity ligation assays (isPLA). The protocol combines isPLA in microtiter wells with automated microscopy and computer-based image analysis whereby specific protein phosphorylations and interactions are digitally recorded in cells, along with measurements of morphological features. We demonstrate how this platform can improve analysis of cellular signaling by investigating TGF-b responsive Smad2 linker phosphorylations and complex formations over time and across millions of individual cells. We depict single cell responses in relation to e.g. local cell crowding and cell cycle progression via measurements of DNA content and nuclear size. Finally, we illustrate the application of the protocol for demonstrating drug effects by screening a library of phosphatase inhibitors. In summary, our approach expands the scope for image-based single cell analyses by combining observations of protein interactions and modifications with morphological details of individual cells at high throughput.
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| 64. |
- Ma, Taoran, et al.
(författare)
-
Self-organizing nanostructured lamellar (Ti,Zr)C - A superhard mixed carbide
- 2015
-
Ingår i: International journal of refractory metals & hard materials. - : Elsevier BV. - 0263-4368. ; 51, s. 25-28
-
Tidskriftsartikel (refereegranskat)abstract
- A nanoindentation and first-principles calculation study of a self-organizing nanostructured lamellar (Ti,Zr)C powder has been performed. The nanoindentation measurements reveal that the hardness of the carbide is comparable to the hardest transition metal carbides that have been reported previously. The origin of the super-high hardness is postulated to be due to the inherent bond strength and the large coherency strains that are generated when the carbide demixes within the miscibility gap. The high hardness is maintained at a high level even after 500 h aging treatment at 1300°C. Therefore, it is believed that the new superhard mixed carbide has a high potential in various engineering applications such as in bulk cemented carbide and cermet cutting tools, and in surface coatings.
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| 65. |
- Masood, Ansar, 1983-, et al.
(författare)
-
Distinct Plasticity of Biocompatible Ti-Zr-Cu-Pd-Sn Bulk Metallic Glass
-
Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- We have developed Ti-Zr-Cu-Pd-Sn bulk metallic glass without toxic elements which exhibits distinct plasticity (~12.6%) by revealing strain hardening before failure. Specimens performed under compression tests do not show any crystalline phases which usually enhance plasticity by branching or restricting the rapid propagation of shear bands. Along with excellent mechanical properties alloy exhibits appreciably high bulk forming ability, GFA, with large supercooled regime (~56K) and as a consequence cylindrical rods of at least 7mm were fabricated directly by Cu-mold casting. The combination of such mechanical properties and appreciably high bulk forming ability makes it a potential candidate for biomedical applications.
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| 66. |
- Masood Hafez Haghighat, Seyed, et al.
(författare)
-
Discrete Models : Down to Atoms and Electrons
- 2016
-
Ingår i: Handbook of Software Solutions for ICME. - Weinheim, Germany : Wiley-VCH Verlagsgesellschaft. - 9783527339020 ; , s. 385-431
-
Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- This chapter reviews some computational methods and software tools available for modeling materials at small scales where the matter exhibits its discrete nature. Discrete models operate on different levels, ranging from the electronic structure and atomistic level to the mesoscopic level at which the discrete objects are molecules or crystal defects rather than the individual atoms. The chapter considers the main methods of atomistic modeling, molecular dynamics (MD) and Monte Carlo (MC), and quantum mechanics (QM) electronic structure methods. It discusses the effective potentials, interatomic interactions, and force fields (FFs), which serve the purpose of passing the physical information between the different levels of modeling. Two atomistic methods are also reviewed. Kinetic MC (KMC) is first introduced as a technique able to link the nanoscale and mesoscale domains but relying heavily on atomistic parameterizations. Then, the powerful MD technique is explained, and some links between both techniques are also hinted.
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| 67. |
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| 68. |
- Masood, Talha Bin, et al.
(författare)
-
An Overview of the Topology ToolKit
- 2021
-
Ingår i: Topological Methods in Data Analysis and Visualization VI. - Cham : Springer. - 9783030835002 - 9783030834999 ; , s. 327-342
-
Bokkapitel (refereegranskat)abstract
- This software paper gives an overview of the features supported by the Topology ToolKitTopology ToolKit (TTK), which is an  Open-sourceopen-source library for  Topological data analysistopological data analysis (TDA). TTK implements, in a generic and efficient way, a substantial collection of reference algorithms in TDA. Since its initial public release in 2017, both its user and developer bases have grown, resulting in a significant increase in the number of supported features. In contrast to the original paper introducing TTK [40] (which detailed the core algorithms and data structures of TTK), the purpose of this Softwaresoftware paper is to describe the list of features currently supported by TTK, ranging from image segmentation tools to advanced topological analysis of high-dimensional data, with concrete usage examples available on the TTK website [42].
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| 69. |
- Munir, M. Adeel, et al.
(författare)
-
Blockchain Adoption for Sustainable Supply Chain Management : Economic, Environmental, and Social Perspectives
- 2022
-
Ingår i: Frontiers in Energy Research. - : Frontiers Media S.A.. - 2296-598X. ; 10
-
Tidskriftsartikel (refereegranskat)abstract
- Due to the rapid increase in environmental degradation and depletion of natural resources, the focus of researchers is shifted from economic to socio-environmental problems. Blockchain is a disruptive technology that has the potential to restructure the entire supply chain for sustainable practices. Blockchain is a distributed ledger that provides a digital database for recording all the transactions of the supply chain. The main purpose of this research is to explore the literature relevant to blockchain for sustainable supply chain management. The focus of this review is on the sustainability of the blockchain-based supply chain concerning environmental conservation, social equality, and governance effectiveness. Using a systematic literature review, a total of 136 articles were evaluated and categorized according to the triple bottom-line aspects of sustainability. Challenges and barriers during blockchain adoption in different industrial sectors such as aviation, shipping, agriculture and food, manufacturing, automotive, pharmaceutical, and textile industries were critically examined. This study has not only explored the economic, environmental, and social impacts of blockchain but also highlighted the emerging trends in a circular supply chain with current developments of advanced technologies along with their critical success factors. Furthermore, research areas and gaps in the existing research are discussed, and future research directions are suggested. The findings of this study show that blockchain has the potential to revolutionize the entire supply chain from a sustainability perspective. Blockchain will not only improve the economic sustainability of the supply chain through effective traceability, enhanced visibility through information sharing, transparency in processes, and decentralization of the entire structure but also will help in achieving environmental and social sustainability through resource efficiency, accountability, smart contracts, trust development, and fraud prevention. The study will be helpful for managers and practitioners to understand the procedure of blockchain adoption and to increase the probability of its successful implementation to develop a sustainable supply chain network.
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| 70. |
- Nagar, Sandeep, et al.
(författare)
-
A new material for Magneto-Optical applications : (Fe72B24Nb4)95.5Y4.5 glassy thin film
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Magneto-Optical properties have been investigated for new kind of glassy thin films. 5, 8, 25 and 30 nm (Fe72B24Nb4)95.5Y4.5 thin films were fabricated using Pulsed LASER deposition (PLD) technique. These thin films were then compared to Fe thin films of same thickness deposited under similar conditions. Using inversion of spectroscopic transmittance and reflectance spectra in the wavelength range 300-2500nm, optical constant ε2(imaginary part of dielectric constant) was found. The optical properties resemble those of other transition metals and their alloys, being mainly determined by interband transitions in the studied wavelength range. The free electron contribution is not significant in this region, which is in line with their low electrical conductivity. These thin films also show large moment (~372.5emu/cc) and soft magnetic properties (coercivity of ~15 Gauss). Being glassy in nature, they can be easily fabricated on any kind of substrate and can tolerate high temperatures (Glass transition temperature for bulk material is close to 700°C [1]) without changing physical properties. Epitaxial and defect free growth of thin films are critical parameters for thin film fabrication. These can be avoided using amorphous materials hence (Fe72B24Nb4)95.5Y4.5 thin films has potential for new functional thin film structures and composites for magneto-optic applications.
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| 71. |
- Nagar, Sandeep, et al.
(författare)
-
Magnetic and electronic properties of glassy (Fe72B24Nb4)95.5Y4.5 ferromagneticthin films fabricated using Pulsed Laser Deposition technique
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Magnetic and electrical properties have been studied for (Fe72B24Nb4)95.5Y4.5 ferromagnetic thin films fabricated using Pulsed Laser Deposition technique. Magnetic characterization shows that these thin films are soft ferromagnetic at room temperature with high saturating magnetic moment (averaged at 372.5 emu/cc). Magnetic data indicates mixed orientation of magnetic moments where mostly in-plane orientation of magnetic moments along with a minority contribution from out of plane magnetic moments. This arrangement of mixed orientation of magnetic moment is attributed to energy of LASER beam used for deposition. Electrical characterization show peculiar thickness dependence of electrical transport and corresponding optical behavior. Temperature dependence of resistivity shows a negative temperature coefficient of resistance which is characteristic of amorphous state. Mott and Efros-Shklovskii hopping mechanism were found to work under different temperature and thickness regimes for these thin films. Since these thin films are amorphous hence their physical properties are independent of choice of substrate and hence present a major advantage while fabricating magneto-optic devices for NEMS.
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| 72. |
- Schiffer, Tomas A., et al.
(författare)
-
Mitochondrial Respiration-Dependent ANT2-UCP2 Interaction
- 2022
-
Ingår i: Frontiers in Physiology. - : Frontiers Media S.A.. - 1664-042X. ; 13
-
Tidskriftsartikel (refereegranskat)abstract
- Adenine nucleotide translocases (ANTs) and uncoupling proteins (UCPs) are known to facilitate proton leak across the inner mitochondrial membrane. However, it remains to be unravelled whether UCP2/3 contribute to significant amount of proton leak in vivo. Reports are indicative of UCP2 dependent proton-coupled efflux of C4 metabolites from the mitochondrial matrix. Previous studies have suggested that UCP2/3 knockdown (KD) contributes to increased ANT-dependent proton leak. Here we investigated the hypothesis that interaction exists between the UCP2 and ANT2 proteins, and that such interaction is regulated by the cellular metabolic demand. Protein-protein interaction was evaluated using reciprocal co-immunoprecipitation and in situ proximity ligation assay. KD of ANT2 and UCP2 was performed by siRNA in human embryonic kidney cells 293A (HEK293A) cells. Mitochondrial and cellular respiration was measured by high-resolution respirometry. ANT2-UCP2 interaction was demonstrated, and this was dependent on cellular metabolism. Inhibition of ATP synthase promoted ANT2-UCP2 interaction whereas high cellular respiration, induced by adding the mitochondrial uncoupler FCCP, prevented interaction. UCP2 KD contributed to increased carboxyatractyloside (CATR) sensitive proton leak, whereas ANT2 and UCP2 double KD reduced CATR sensitive proton leak, compared to UCP2 KD. Furthermore, proton leak was reduced in double KD compared to UCP2 KD. In conclusion, our results show that there is an interaction between ANT2-UCP2, which appears to be dynamically regulated by mitochondrial respiratory activity. This may have implications in the regulation of mitochondrial efficiency or cellular substrate utilization as increased activity of UCP2 may promote a switch from glucose to fatty acid metabolism.
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| 73. |
- Shahid, M., et al.
(författare)
-
Serratia sp. CP-13 augments the growth of cadmium (Cd)-stressed Linum usitatissimum L. by limited Cd uptake, enhanced nutrient acquisition and antioxidative potential
- 2019
-
Ingår i: Journal of Applied Microbiology. - : Oxford University Press (OUP). - 1364-5072 .- 1365-2672. ; 126:6, s. 1708-1721
-
Tidskriftsartikel (refereegranskat)abstract
- Aims: The current study was aimed to evaluate the beneficial effects and bioremediation potential of a Cd-tolerant bacterial strain, Serratia sp. CP-13, on the physiological and biochemical functions of Linum usitatissimum L., under Cd stress.Methods and Results: The bacterial strain was isolated from the wastewater collection point of Chakera, Faisalabad, Pakistan, as this place contains industrial wastewater of the Faisalabad region. The Serratia sp. CP-13, identified through 16S rRNA gene sequence analysis, exhibited a significant phyto-beneficial potential in terms of in vitro inorganic phosphate solubilization, indole-3-acetic acid production and 1-aminocyclopropane-1-carboxylic acid deaminase activity. Effects of Serratia sp. CP-13 inoculation on L. usitatissimum were evaluated by growing the plants in CdCl2 (0, 5 or 10 mg kg(-1) dry soil)-spiked soil. Without inoculation of Serratia sp. CP-13, Cd stress significantly reduced the plant biomass as well as the quantity of proteins and photosynthetic pigments due to enhanced H2O2, malondialdehyde (MDA) contents and impaired nutrient homeostasis. Subsequently, Serratia sp. CP-13 increased the plant fresh and dry biomass, plant antioxidation capacity, whereas it decreased the lipid peroxidation under Cd stress. In parallel, Serratia sp. inoculation assisted the Cd-stressed plants to maintain an optimum level of nutrients (K, Ca, P, Mg, Fe and Mn).Conclusions: The isolated bacterial strain (Serratia sp. CP-13) when applied to Cd-stressed L. usitatissimum inhibited the Cd uptake, reduced Cd-induced lipid peroxidation, maintained the optimum level of nutrients and thereby, enhanced L. usitatissimum growth. The analysis of bio-concentration and translocation factor revealed that L. usitatissimum with Serratia sp. CP-13 inoculation sequestered Cd in plant rhizospheric zone.Significance and Impact of the Study: Serratia sp. CP-13 inoculation is a potential candidate for the development of low Cd-accumulating linseed and could be used for phytostabilization of Cd-contaminated rhizosphere/soil colloids.
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