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- Malmström, P, et al.
(författare)
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Countercurrent distribution of lymphocytes from human peripheral blood in an aqueous two-phase system. : II. Separation into subsets of lymphocytes with distinctive functions
- 1980
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Ingår i: Cellular Immunology. - : Elsevier BV. - 0008-8749. ; 53, s. 51-64
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Tidskriftsartikel (refereegranskat)abstract
- Lymphocytes from human peripheral blood were separated by Countercurrent distribution (CCD) in a charged aqueous two-phase system composed of Dextran T 500 and polyethylene glycol 6000. Maximal responses to the T-cell mitogens phytohemagglutinin and concanavalin A were detected in a part of the distribution corresponding to the area where the highest percentage of E rosetting cells were seen. Antibody-dependent cellular cytotoxicity was mediated by lymphocytes located in a restricted part of the distribution separate from the majority of T lymphocytes. Lymphocytes with natural killer activity against K 562, adherent melanoma cells, and fibroblasts were detected in the same region. This area of the distribution also contained the peak of lymphocytes with high-affinity Fc receptors. It was further investigated whether affinity separation may be possible in two-phase systems on the basis of cell to cell interaction. Affinity CCD utilizing the interaction between sheep erythrocytes (SRBC) and T lymphocytes was shown to redistribute the majority of T cells into the area in which SRBC were located, while other lymphocytes were not affected. Lymphocytes mediating natural killing to adherent target cells were not redistributed, indicating that they lack high-affinity receptors for SRBC.
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