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Träfflista för sökning "L773:0165 2478 OR L773:1879 0542 srt2:(1986-1989)"

Sökning: L773:0165 2478 OR L773:1879 0542 > (1986-1989)

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1.
  • Nilsson, B, et al. (författare)
  • SDS denaturation of complement factor C3 as a model for allosteric modifications occurring during C3b binding : demonstration of a profound conformational change by means of circular dichroism and quantitative immunoprecipitation.
  • 1986
  • Ingår i: Immunology Letters. - 0165-2478 .- 1879-0542. ; 13:1-2, s. 11-14
  • Tidskriftsartikel (refereegranskat)abstract
    • The antigenic expression of bound but not fluid-phase C3b closely resembles that of sodium dodecyl sulphate (SDS) denatured C3. For this reason, denatured C3 has been used in this study as a model to characterize the conformational changes associated with bound C3b. It was shown in circular dichroism in the far UV spectrum that profound changes in the secondary structure occurred in denatured C3. Furthermore, quantitation by immunoprecipitation of the previously observed antigenic changes during denaturation demonstrated that C3 lost 2/3 of the antigens associated with native C3 whereas 1/3 were stable. The lost antigens were completely replaced by antigens that are specific for denatured and bound C3. We postulate that the binding of C3b is accompanied by a profound conformational change distinctive of that observed in fluid-phase C3b.
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2.
  • Yefenof, E, et al. (författare)
  • Ligands of CR2 do not interfere with C3 fragment fixation or enhanced NK sensitivity of Raji cells treated with human serum.
  • 1989
  • Ingår i: Immunology Letters. - 0165-2478 .- 1879-0542. ; 21:4, s. 303-306
  • Tidskriftsartikel (refereegranskat)abstract
    • Raji cells activate the alternative complement pathway (ACP) and fix C3 fragments when incubated in human serum (HS). Earlier experiments have shown that CR2 molecules are involved in this phenomenon and the opsonized cells have elevated sensitivity to the lytic effect of CR3-bearing NK cells. We show here that Raji cells treated with CR2 site-specific ligands, (C3d, OKB-7 and HB-5 mAbs, and a synthetic peptide which binds to CR2) generated and bound C3 fragments after exposure to HS. The elevated lytic sensitivity of HS-treated cells was not altered by the presence of the various CR2 ligands. Thus, the membrane-bound C3 fragments are not fixed at the C3dg receptor binding site.
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  • Resultat 1-2 av 2
Typ av publikation
tidskriftsartikel (2)
Typ av innehåll
refereegranskat (2)
Författare/redaktör
Nilsson, B (2)
Klein, E (1)
Nilsson, U R (1)
Ramos, O F (1)
Yefenof, E (1)
Lärosäte
Uppsala universitet (2)
Språk
Engelska (2)

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