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Träfflista för sökning "L773:1095 9130 OR L773:1046 2023 srt2:(2000-2004)"

Sökning: L773:1095 9130 OR L773:1046 2023 > (2000-2004)

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1.
  • Kanno, T, et al. (författare)
  • Large dense-core vesicle exocytosis in pancreatic beta-cells monitored by capacitance measurements
  • 2004
  • Ingår i: Methods. - : Elsevier BV. - 1095-9130 .- 1046-2023. ; 33:4, s. 302-311
  • Tidskriftsartikel (refereegranskat)abstract
    • This article discusses the currently used methodologies for monitoring exocytosis as changes in cell capacitance. Details are given on composition of solutions, experimental protocols, and how the observed responses can be interpreted physiologically. The concepts are illustrated by examples from our own work on insulin-releasing pancreatic P-cells. Finally, we consider the feasibility of applying capacitance measurements to endocrine cells in intact pancreatic islets, where the cells are electrically coupled to each other. (C) 2004 Elsevier Inc. All rights reserved.
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  • Sjöbring, Ulf, et al. (författare)
  • Analysis of plasminogen-binding M proteins of Streptococcus pyogenes.
  • 2000
  • Ingår i: Methods. - 1095-9130. ; 21:2, s. 143-150
  • Tidskriftsartikel (refereegranskat)abstract
    • Group A streptococci are common human pathogens that cause a variety of infections. They express M proteins which are important cell wall-bound type-specific virulence factors. We have found that a set of strains, associated primarily with skin infections, express M proteins that bind plasminogen and plasmin with high affinity. The binding is mediated by a 13-amino-acid internal repeated sequence located in the N-terminal surface-exposed portion of these M proteins. This sequence binds to kringle 2 in plasminogen, a domain that is not involved in the interaction with streptokinase, a potent group A streptococcal activator of plasminogen. It could be demonstrated that plasminogen, absorbed from plasma by growing group A streptococci expressing the plasminogen-binding M proteins, could be activated by exogenous and endogenous streptokinase, thereby providing the bacteria with a surface-associated enzyme that could act on the tissue barriers in the infected host.
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