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Selected ion monito...
Selected ion monitoring of glycospingolipid mixtures. Identification of several blood group type glycolipids in the small intestine of an individual rabbit.
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- Breimer, Michael, 1951 (författare)
- Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine
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- Hansson, Gunnar C., 1951 (författare)
- Gothenburg University,Göteborgs universitet,Medicinska institutionen,Department medicine
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Karlsson, K A (författare)
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Leffler, H (författare)
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Pimlott, W (författare)
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Samuelsson, B E (författare)
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(creator_code:org_t)
- Wiley, 1979
- 1979
- Engelska.
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Ingår i: Biomedical mass spectrometry. - : Wiley. - 0306-042X .- 1096-9888. ; 6:6, s. 231-41
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A novel application of selected ion monitoring was used for a mixture of non-acid glycosphingolipids of one rabbit small intestine. Earlier studies of permethylated and permethylated-reduced (LiAIH4) derivatives of model compounds have revealed a specificity and abundance of saccharide ions (terminal monosaccharide(s), disaccharide, trisaccharide, etc., and all sugars plus fatty acid) and of ceramide fragments that permit a conclusive detection of separate glycolipid species in a mixture. The sample (50-200 micrograms) was evaporated slowly (1-5 degrees C min-1 from 150-350 degrees C) from the direct inlet probe of an MS 902 mass spectrometer (electron ionization). Mass spectra with fragments up to about m/z 200 were collected on-line by a computer system. A successive partial separation was obtained for glycolipids with from one up to seven sugars. The structures of eight different compounds were identified. They all had 16:0, 22:0 and 24:0 2-hydroxy fatty acids and 18:0 trihydroxy base (phytosphingosine) as major ceramide components. The dominating complex glycolipid was a hexaglycosylceramide with a blood group B type of sequence. A blood group A type sequence was found in a second hexaglycosylceramide. In support of this, the native mixture showed blood group A and B activity. An intense peak, m/z 182, collected from methylated derivatives were evidence for a dominating type 2 carbohydrate chain of the core tetrasaccharide.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- Animals
- Blood Group Antigens
- Chromatography
- Thin Layer
- Computers
- Glycolipids
- analysis
- Glycosphingolipids
- analysis
- Intestine
- Small
- analysis
- Lewis Blood-Group System
- Mass Spectrometry
- Rabbits
- Trihexosylceramides
- analysis
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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