| 1. |
- Carlsson, Peter, et al.
(författare)
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In vitro complementation of Bacillus subtilis and Escherichia coli 2-oxoglutarate dehydrogenase complex mutants and genetic mapping of B. subtilis citK and citM mutations
- 1986
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Ingår i: FEMS Microbiology Letters. - : Oxford University Press (OUP). - 1574-6968 .- 0378-1097. ; 37:3, s. 373-378
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Tidskriftsartikel (refereegranskat)abstract
- AbstractThe 2-oxoglutarate dehydrogenase complex of the tricarboxylic acid cycle (TCA) consists of multiple copies of 3 different subenzymes; E1, E2 and E3. The E3 subenzyme is also a component of the pyruvate dehydrogenase complex. Bacillus subtilis 2-oxoglutarate dehydrogenase mutants were studied. The mutants defective in E1, E2 and E3 subenzyme activity, respectively, could be separated into 3 groups by biochemical complementation analyses. The groups correspond to the citK, citM and citL genes. A B. subtilis subenzyme defect, probably E1, could be complemented with the corresponding Escherichia coli wild-type subenzyme and vice versa. Mutations in citK and citM are closely linked. The gene order kauA--- ---citK-citM was determined from 3-factor transformation crosses. It is concluded that the gene organization and the subenzyme structure of the 2-oxoglutarate dehydrogenase complex are similar in B. subtilis and E. coli.
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| 2. |
- Fridén, H, et al.
(författare)
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Deletion of the Bacillus subtilis sdh operon
- 1987
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Ingår i: FEMS Microbiology Letters. - : Oxford University Press (OUP). - 1574-6968 .- 0378-1097. ; 41:2, s. 206-206
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Tidskriftsartikel (refereegranskat)abstract
- Plasmid pKIM2 carries the Bacillus subtilis sdh operon and adjacent regions of the bacterial chromosome. The plasmid replicates in Escherichia coli but not in B. subtilis. Different portions of the sdh operon were removed from pKIM2 and replaced by a cat gene derived from pC194. A series of plasmids carrying sdh deletions was thus derived. Plasmid DNA was linearized at restriction sites within the vector part and used to transform B. subtilis to chloramphenicol resistance. The majority of the transformants had a succinate dehydrogenase-negative phenotype and were deleted in the sdh operon as verified by Southern blotting. The B. subtilis deletion mutants were used to determine the functional integrity of cloned sdh genes.
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| 3. |
- Daeschel, M.A., et al.
(författare)
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Microbial ecology of fermenting plant materials
- 1987
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Ingår i: FEMS Microbiology Letters. - 0378-1097 .- 1574-6968. ; 46:3, s. 357-367
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Tidskriftsartikel (refereegranskat)abstract
- The lactic acid fermentation of plant materials is presented from an ecological perspective emphasizing microbial interactions and their influence on the production of fermented plant foods and silage. The plant lactic acid bacteria are discussed in terms of evolution; epiphytic function; physical distribution within fermented material; substrates and products; microbial sequences in fermentation; interactions among species; pure culture fermentation; and starter culture development. © 1987.
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| 4. |
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| 5. |
- Petricek, M, et al.
(författare)
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The structural gene for aspartokinase II in Bacillus subtilis is closely linked to the sdh operon
- 1989
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Ingår i: FEMS Microbiology Letters. - 1574-6968. ; 61:1-2, s. 85-87
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Tidskriftsartikel (refereegranskat)abstract
- The aecA and aecB loci map at 250 and 290 degrees, respectively, on the Bacillus subtilis chromosomal genetic map. The aecB locus has been proposed as the structural gene for aspartokinase II. From DNA sequence analyses and comparisons to the sequence of the aspartokinase II gene, it can be concluded that the structural gene for aspartokinase II is located close to sdh at 250 degrees and cannot be aecB. A detailed map over 7 kbp in the 250 degree region is presented.
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