| 1. |
- Naredi, S., et al.
(författare)
-
An outcome study of severe traumatic head injury using the "Lund therapy" with low-dose prostacyclin
- 2001
-
Ingår i: Acta Anaesthesiologica Scandinavica. - : Wiley-Blackwell. - 0001-5172 .- 1399-6576. ; 45:4, s. 402-406
-
Tidskriftsartikel (refereegranskat)abstract
- Background: There are two independent head injury outcome studies using the “Lund concept”, and both showed a mortality rate of about 10%, and a favourable outcome (Glasgow outcome scale, GOS 4 and 5) of about 70%. The Lund concept aims at controlling intracranial pressure, and improving microcirculation around contusions. Intracranial pressure is controlled by maintaining a normal colloid osmotic pressure and reducing the hydrostatic capillary pressure. Microcirculation is improved by ensuring strict normovolaemia and reducing sympathetic discharge. The endogenous substance prostacyclin with its antiaggregatory/antiadhesive effects may further improve microcirculation, which finds support from a microdialysis‐based clinical study and an experimental brain trauma study. The present clinical outcome study aims at evaluating whether the previously obtained good outcome with the Lund therapy can be reproduced, and whether the addition of prostacyclin has any adverse side‐effects.Methods: All 31 consecutive patients with severe head injury, Glasgow coma scale (GCS) ≤8, admitted to the University Hospital of Umeå during 1998 were included. The Lund therapy including prostacyclin infusion for the first three days at a dose of 0.5 ng kg−1 min−1. Outcome was evaluated according to the GOS >10 months after the injury.Results: One patient died, another suffered vegetative state and 7 severe disability. Of the 22 patients with favourable outcome, 19 showed good recovery and 3 moderate disability. No adverse side‐effects of prostacyclin were observed.Conclusion: The outcome results from previous studies using the Lund therapy were reproduced, and no adverse side‐effects of low‐dose prostacyclin were observed.
|
|
| 2. |
|
|
| 3. |
- Sabri, N, et al.
(författare)
-
Evidence for a Posttranscription Role of a TFIIIC-like Protein in Chironomus tentans.
- 2002
-
Ingår i: Molecular Biology of the Cell. - : American Society for Cell Biology (ASCB). - 1059-1524 .- 1939-4586. ; 13, s. 1765-1777
-
Tidskriftsartikel (refereegranskat)abstract
- We have cloned and sequenced a cDNA that encodes for a nuclear protein of 238 kDa in the dipteran Chironomus tentans. This protein, that we call p2D10, is structurally similar to the α subunit of the general transcription factor TFIIIC. Using immunoelectron microscopy we have shown that a fraction of p2D10 is located at sites of transcription, which is consistent with a possible role of this protein in transcription initiation. We have also found that a large fraction of p2D10 is located in the nucleoplasm and in the nuclear pore complexes. Using gel filtration chromatography and coimmunoprecipitation methods, we have identified and characterized two p2D10-containing complexes that differ in molecular mass and composition. The heavy p2D10-containing complex contains at least one other component of the TFIIIC complex, TFIIIC-ε. Based on its molecular mass and composition, the heavy p2D10-containing complex may be the Pol III holoenzyme. The light p2D10-containing complex contains RNA together with at least two proteins that are thought to be involved in mRNA trafficking, RAE1 and hrp65. The observations reported here suggest that this new TFIIIC-α-like protein is involved in posttranscriptional steps of premRNA metabolism in Chironomus tentans.
|
|
| 4. |
- Östlund, Jenny A., et al.
(författare)
-
Component-resolved diffusion in multicomponent mixtures. A case study of high-field PGSE-NMR self-diffusion measurements in asphaltene/naphthenic acid/solvent systems
- 2004
-
Ingår i: Energy & Fuels. - : American Chemical Society (ACS). - 0887-0624 .- 1520-5029. ; 18:2, s. 531-538
-
Tidskriftsartikel (refereegranskat)abstract
- The diffusion of a multitude of different-sized molecules in various asphaltene mixtures has been measured by means of the pulsed gradient spin-echo nuclear magnetic resonance (PGSE-NMR) self-diffusion technique. The multicomponent mixtures studied contained mixtures of polydisperse asphaltenes and monodisperse naphthenic acid in solvent. Spectra obtained from a high-field PGSE-NMR self-diffusion experiment on these systems showed that the peaks from all components were almost completely overlapping, making a standard least-squares fit approach for evaluating individual self-diffusion coefficients from the decrease in signal intensity quite difficult. However, to study the details of the molecular interactions that occur between asphaltenes and naphthenic acids, the experimental data must be such that component-resolved information is obtained. Hence, with the purpose to obtain a robust evaluation procedure, with regard to obtaining component-resolved diffusion coefficients, a standard least-squares fit approach was tested against a global least-squares approach. The global least-squares evaluation was performed using a program called CORE (component-resolved spectroscopy). CORE not only fits predetermined functions to specific peak intensities or areas obtained from the diffusion experiments, but also considers the full information in the spectra. That is, the intensity at each frequency in the spectra is considered and the band shapes for all components in the mixture can be calculated. The calculated band shapes are thus a good test of the validity of the results for the diffusion coefficients. In this work, the band shapes were calculated and the diffusion for each individual component in the samples was extracted. The band shapes were observed to be in close agreement with the spectra obtained for the pure components. In addition, the diffusion coefficients, as obtained from CORE and from the least-squares fit method, were in close agreement, although some discrepancies were noted.
|
|
