| 1. |
- af Klinteberg, C, et al.
(författare)
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Laser-induced fluorescence diagnostics of basal cell carcinomas of the skin following topical ALA application
- 1996
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Ingår i: Optical Biopsies and Microscopic Techniques, Proceedings of. - : SPIE. - 0819423289 ; 2926, s. 32-40
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Konferensbidrag (refereegranskat)abstract
- Fourteen patients with superficial basal cell carcinomas (BCCs) and fifteen patients with nodular BCCs were investigated by means of laser-induced fluorescence (LIF) in connection with photodynamic therapy (PDT). Topical application of delta-amino levulinic acid (ALA) was performed six hours prior to the treatment session. Fluorescence spectra were recorded, using a point-monitoring system with an excitation wavelength of 405 nm. The measurements were performed in scans over the lesion and the surrounding normal skin before application of ALA, and immediately before and after the laser treatment. The selective uptake of the photosensitiser resulted in a fluorescence intensity ratio of 2.4:1 for superficial BCCs and 2.5:1 for nodular BCCs. If the fluorescence intensity was divided by the autofluorescence, this resulted in a contrast enhancement of about a factor 6 for tumour tissue. In seven patients (five with nodular BCC and two with superficial BCC), additional fluorescence measurements were performed two and four hours following the ALA application, and two hours after the PDT procedure. Thus, the kinetics of the transformation of ACA to protoporphyrin IX (PpIX) could be followed, which indicated that the synthesis of PpIX was more rapid in the tumour than in the normal tissue. After four hours, the PpIX level inside the tumour was saturated, while there still was an accumulation in the surrounding skin. The highest contrast between tumour and normal skin was reached within two hours after the ALA application.
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| 2. |
- Andersson, SGE, et al.
(författare)
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Codon usage and base composition in Rickettsia prowazekii
- 1996
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Ingår i: JOURNAL OF MOLECULAR EVOLUTION. - : SPRINGER VERLAG. - 0022-2844. ; 42:5, s. 525-536
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Codon usage and base composition in sequences from the A + T-rich genome of Rickettsia prowazekii, a member of the alpha Proteobacteria, have been investigated. Synonymous codon usage patterns are roughly similar among genes, even though the data set incl
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| 3. |
- Andersson, SGE, et al.
(författare)
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Codon usage in the Mycobacterium tuberculosis complex
- 1996
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Ingår i: MICROBIOLOGY-UK. - : SOC GENERAL MICROBIOLOGY. - 1350-0872. ; 142, s. 915-925
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The usage of alternative synonymous codons in Mycobacterium tuberculosis (and M. bovis) genes has been investigated. This species is a member of the high-Ci + C Gram-positive bacteria, with a genomic G + C content around 65 mol%, This G + C-richness is re
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| 4. |
- Filippidis, G, et al.
(författare)
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Effect of liquid nitrogen and formalin-based conservation in the in-vitro measurement of laser-induced fluorescence of peripheral vascular tissue
- 1997
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Ingår i: OPTICAL BIOPSIES AND MICROSCOPIC TECHNIQUES II. - : SPIE. - 1996-756X .- 0277-786X. - 0819426296 ; 3197, s. 27-31
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Konferensbidrag (refereegranskat)abstract
- In order to investigate the effects of conservation in liquid nitrogen and formalin on peripheral vascular tissue (abdominal aortic, femoral and flank artery tissue) laser-induced fluorescence spectra were recorded during the exposure of these tissues to He-Cd radiation (442 nm). The spectral distribution of tissue fluorescence allowed the development of simple algorithms based on the intensity difference in order to discriminate the tissue samples when they were fresh and after they were stored for 24 and 48 hours in liquid nitrogen or formalin.
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