| 1. |
- Anderson, Cynthia M., et al.
(författare)
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Permanent Genetic Resources added to Molecular Ecology Resources Database 1 December 2009-31 January 2010
- 2010
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Ingår i: Molecular Ecology Resources. - : Wiley. - 1755-098X .- 1755-0998. ; 10:3, s. 576-579
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Tidskriftsartikel (refereegranskat)abstract
- This article documents the addition of 220 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco, Phragmidium violaceum, Pistacia vera, and Thunnus thynnus. These loci were cross-tested on the following species: Allanblackia gabonensis, Allanblackia stanerana, Neoceratitis cyanescens, Dacus ciliatus, Dacus demmerezi, Bactrocera zonata, Ceratitis capitata, Ceratitis rosa, Ceratits catoirii, Dacus punctatifrons, Ephydatia mulleri, Spongilla lacustris, Geodia cydonium, Axinella sp., Ischnura graellsii, Ischnura ramburii, Ischnura pumilio, Pistacia integerrima and Pistacia terebinthus.
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| 2. |
- Balao da Silva, C M., et al.
(författare)
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Sex sorting increases the permeability of the membrane of stallion spermatozoa
- 2013
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Ingår i: Animal Reproduction Science. - : Elsevier Masson. - 0378-4320 .- 1873-2232. ; 138:3-4, s. 241-251
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Tidskriftsartikel (refereegranskat)abstract
- At present, the only repeatable means of selecting the sex of offspring is the Beltsville semen sorting technology using flow cytometry (FC). This technology has reached commercial status in the bovine industry and substantial advances have occurred recently in swine and ovine species. In the equine species, however, the technology is not as well developed. To better understand the changes induced in stallion spermatozoa during the sorting procedure, pooled sperm samples were sorted: sperm motility and kinematics were assessed using computer assisted sperm analysis, sperm membrane integrity was assessed using the YoPro-1 assay, while plasmalemmal stability and lipid architecture were assessed using Merocyanine 540/SYTOX green and Annexin-V, respectively. Lipid peroxidation was also investigated with the probe Bodipy(581/591)-C11. All assays were performed shortly after collection, after incubation and after sex sorting using FC. In order to characterize potential molecular mechanisms implicated in sperm damage, an apoptosis protein antibody dot plot array analysis was performed before and after sorting. While the percentage of total motile sperm remained unchanged, sex sorting reduced the percentages of progressive motile spermatozoa and of rapid spermatozoa as well as curvilinear velocity (VCL). Sperm membranes responded to sorting with an increase in the percentage of YoPro-1 positive cells, suggesting the sorted spermatozoa had a reduced energy status that was confirmed by measuring intracellular ATP content.
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| 3. |
- Macias Garcia, B, et al.
(författare)
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Toxicity of glycerol for the stallion spermatozoa: Effects on membrane integrity and cytoskeleton, lipid peroxidation and mitochondrial membrane potential
- 2012
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Ingår i: Theriogenology. - : Elsevier. - 0093-691X .- 1879-3231. ; 77:7, s. 1280-1289
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Tidskriftsartikel (refereegranskat)abstract
- Glycerol is, to date, the most widely used cryoprotectant to freeze stallion spermatozoa at concentrations between 2% and 5%. Cryoprotectant toxicity has been claimed to be the single most limiting factor for the success of cryopreservation. In order to evaluate the toxic effects of the concentrations of glycerol used in practice, stallion spermatozoa were incubated in Biggers Whitten and Whittingham (BWW) media supplemented with 0%, 0.5%, 1.5%, 2.5%, 3.5%, and 5% glycerol. In two additional experiments, a hyposmotic (75 mOsm/kg) and a hyperosmotic (900 mOsm/kg) control media were included. Sperm parameters evaluated included cell volume, membrane integrity, lipid peroxidation, caspase 3, 7, and 8 activation, mitochondrial membrane potential, and integrity of the cytoskeleton. Glycerol exerted toxicity at concentrations 3.5% and the maximal toxicity was observed at 5%. The actin cytoskeleton was especially sensitive to glycerol presence, inducing rapid F actin depolymerization at concentrations over 1.5%. The sperm membrane and the mitochondria were other structures affected. The toxicity of glycerol is apparently related to osmotic and nonosmotic effects. In view of our results the concentration of glycerol in the freezing media for stallion spermatozoa should not surpass 2.5%.
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| 4. |
- Milone, A. P., et al.
(författare)
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The ACS survey of Galactic globular clusters XII. Photometric binaries along the main sequence
- 2012
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Ingår i: Astronomy & Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 540
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Tidskriftsartikel (refereegranskat)abstract
- Context. The fraction of binary stars is an important ingredient to interpret globular cluster dynamical evolution and their stellar population. Aims. We investigate the properties of main-sequence binaries measured in a uniform photometric sample of 59 Galactic globular clusters that were observed by HST WFC/ACS as a part of the Globular Cluster Treasury project. Methods. We measured the fraction of binaries and the distribution of mass-ratio as a function of radial location within the cluster, from the central core to beyond the half-mass radius. We studied the radial distribution of binary stars, and the distribution of stellar mass ratios. We investigated monovariate relations between the fraction of binaries and the main parameters of their host clusters. Results. We found that in nearly all the clusters, the total fraction of binaries is significantly smaller than the fraction of binaries in the field, with a few exceptions only. Binary stars are significantly more centrally concentrated than single MS stars in most of the clusters studied in this paper. The distribution of the mass ratio is generally flat (for mass-ratio parameter q > 0.5). We found a significant anti-correlation between the binary fraction in a cluster and its absolute luminosity (mass). Some, less significant correlation with the collisional parameter, the central stellar density, and the central velocity dispersion are present. There is no statistically significant relation between the binary fraction and other cluster parameters. We confirm the correlation between the binary fraction and the fraction of blue stragglers in the cluster.
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| 5. |
- Llatser, I., et al.
(författare)
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Characterization of graphene-based nano-antennas in the terahertz band
- 2012
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Ingår i: Proceedings of 6th European Conference on Antennas and Propagation, EuCAP 2012. - : IEEE. - 9781457709180 ; , s. 194-198
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Konferensbidrag (refereegranskat)abstract
- Graphene-enabled wireless communications constitute a novel paradigm which has been proposed to implement wireless communications at the nanoscale. Indeed, graphene-based nano-antennas just a few micrometers in size have been predicted to radiate electromagnetic waves at the terahertz band. In this work, the performance of a graphene-based nano-patch antenna in transmission and reception is numerically analyzed. The resonance frequency of the nano-antenna is calculated as a function of its length and width, both analytically and by simulation. The influence of a dielectric substrate with a variable size, and the position of the patch with respect to the substrate is also evaluated. Finally, the radiation pattern of a graphene-based nano-patch antenna is compared to that of an equivalent metallic antenna. These results will prove useful for designers of future graphene-based nano-antennas, which will enable wireless communications at the nanoscale.
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