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Analysis of small R...
Analysis of small RNA in fission yeast; centromeric siRNAs are potentially generated through a structured RNA
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- Djupedal, Ingela (author)
- Södertörns högskola,Institutionen för livsvetenskaper,Karolinska Institutet
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- Kos-Braun, Isabelle C. (author)
- University of Edinburgh, Edinburgh, UK / Universität Heidelberg, Heidelberg, Germany
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- Mosher, Rebecca A. (author)
- University of Cambridge, Cambridge, UK
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- Söderholm, Niklas (author)
- Karolinska Institutet
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- Simmer, Femke (author)
- University of Edinburgh, Edinburgh, UK
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- Hardcastle, Thomas J. (author)
- University of Cambridge, Cambridge, UK
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- Fender, Aurelie (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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- Heidrich, Nadja (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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- Kagansky, Alexander (author)
- University of Edinburgh, Edinburgh, UK
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- Bayne, Elizabeth (author)
- University of Edinburgh, Edinburgh, UK
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- Wagner, Gerhart E. H. (author)
- Uppsala universitet,Institutionen för cell- och molekylärbiologi
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- Baulcombe, David C. (author)
- University of Cambridge, Cambridge, UK
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- Allshire, Robin C. (author)
- University of Edinburgh, Edinburgh, UK
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- Ekwall, Karl (author)
- Södertörns högskola,Molekylärbiologi,Karolinska Institutet
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(creator_code:org_t)
- 2009-11-26
- 2009
- English.
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In: EMBO Journal. - : Wiley. - 0261-4189 .- 1460-2075. ; 28:24, s. 3832-3844
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Abstract
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- The formation of heterochromatin at the centromeres in fission yeast depends on transcription of the outer repeats. These transcripts are processed into siRNAs that target homologous loci for heterochromatin formation. Here, high throughput sequencing of small RNA provides a comprehensive analysis of centromere-derived small RNAs. We found that the centromeric small RNAs are Dcr1 dependent, carry 50-monophosphates and are associated with Ago1. The majority of centromeric small RNAs originate from two remarkably well-conserved sequences that are present in all centromeres. The high degree of similarity suggests that this non-coding sequence in itself may be of importance. Consistent with this, secondary structure-probing experiments indicate that this centromeric RNA is partially double-stranded and is processed by Dicer in vitro. We further demonstrate the existence of small centromeric RNA in rdp1D cells. Our data suggest a pathway for siRNA generation that is distinct from the well-documented model involving RITS/RDRC. We propose that primary transcripts fold into hairpin-like structures that may be processed by Dcr1 into siRNAs, and that these siRNAs may initiate heterochromatin formation independent of RDRC activity. The EMBO Journal (2009) 28, 3832-3844. doi: 10.1038/emboj.2009.351; Published online 26 November 2009
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- centromeres
- RNAi
- small RNA
- S. pombe
- Biology
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Djupedal, Ingela
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Kos-Braun, Isabe ...
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Mosher, Rebecca ...
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Söderholm, Nikla ...
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Simmer, Femke
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Hardcastle, Thom ...
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Fender, Aurelie
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Heidrich, Nadja
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Kagansky, Alexan ...
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Bayne, Elizabeth
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Wagner, Gerhart ...
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Baulcombe, David ...
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Allshire, Robin ...
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Ekwall, Karl
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Cell Biology
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
- Articles in the publication
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EMBO Journal
- By the university
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Södertörn University
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Karolinska Institutet
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Uppsala University