| 1. |
- Studahl, Marie, 1957, et al.
(författare)
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Cytomegalovirus infection of the CNS in non-compromised patients.
- 1994
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Ingår i: Acta neurologica Scandinavica. - 0001-6314. ; 89:6, s. 451-7
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Tidskriftsartikel (refereegranskat)abstract
- Six non-compromised patients with cytomegalovirus (CMV) associated meningoencephalitis are described. CMV was isolated from the cerebrospinal fluid (CSF) in 2/4 cases, while the diagnosis was based on an 8-fold rise in CMV-specific serum IgG antibodies and intrathecal antibody production against CMV in one case. By the polymerase chain reaction (PCR) CMV DNA was detected in the CSF in 5/5 cases and in serum in 3/4 cases. In one patient who had an Influenza A infection, both CMV and Epstein-Barr virus DNA were detected by PCR in the CSF. In 4 patients possible triggering events could be identified. Symptoms and signs indicating a multifocal brain involvement were present in 4 patients. The outcome was generally favourable except for sequelae in form of slight dysphasia in one case.
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| 2. |
- Bergström, S, et al.
(författare)
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Cloning and sequencing of human kappa-casein cDNA.
- 1992
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Ingår i: DNA Sequence. - 1042-5179 .- 1029-2365. ; 3:4, s. 245-6
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Tidskriftsartikel (refereegranskat)abstract
- A cDNA encoding kappa-casein of human milk was cloned and sequenced. The kappa-casein cDNA was isolated from a lambda gt11 library generated from mRNA prepared from a mammary gland biopsy obtained from a lactating woman. The library was screened with polyclonal rabbit antibodies raised against purified native kappa-casein. The obtained nucleotide sequence contained an ORF sufficient to encode the entire amino acid sequence of a kappa-casein precursor protein consisting of 182 amino acids. This includes a tentative signal peptide of 20 amino acids and a processed protein of 162 amino acids.
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| 3. |
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| 4. |
- Hansson, L, et al.
(författare)
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Expression of human milk beta-casein in Escherichia coli : comparison of recombinant protein with native isoforms.
- 1993
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Ingår i: Protein Expression and Purification. - : Elsevier BV. - 1046-5928 .- 1096-0279. ; 4:5, s. 373-81
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Tidskriftsartikel (refereegranskat)abstract
- Studies on physiological function and on structure-function relationships of human milk beta-casein have been limited. In this study, we have introduced the human beta-casein cDNA into vectors designed for expression in Escherichia coli. The inducible T7-based expression system resulted in high-level expression of recombinant beta-casein. The recombinant beta-casein, localized intracellularly in E. coli, was purified to homogeneity and compared with purified native beta-casein, in particular with respect to phosphorylation. The E. coli-produced beta-casein was found to comigrate with the full-length, nonphosphorylated native human beta-casein isoform on SDS-PAGE. An N-terminal peptide containing all tentative phosphorylation sites was isolated from the recombinant protein and analyzed by mass spectrometry. The molecular mass as well as the migration of this peptide on reversed-phase chromatography confirmed that it was unphosphorylated.
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| 5. |
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| 6. |
- Jahnson, S, et al.
(författare)
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Extent of blood transfusion and cancer-related mortality after cystectomy and urinary diversion for bladder cancer
- 1994
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Ingår i: British Journal of Urology. - : BLACKWELL SCIENCE LTD. - 0007-1331 .- 1365-2176. ; 74:6, s. 779-784
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE:To assess the possible adverse effect of peri-operative blood transfusion on cancer-related survival after radical cystectomy for bladder cancer.PATIENTS AND METHODS:The hospital records of 130 patients treated with cystectomy and urinary diversion for bladder cancer between 1967 and 1986 were retrospectively reviewed.RESULTS:Standard proportional hazards estimation revealed tumour stage and radiation response after pre-operative irradiation to be significantly associated with cancer-related mortality, whereas age, tumour grade or the extent of peri-operative blood transfusion were not. In models which allowed time varying effects a significantly changed effect of blood transfusion (> or = 7 versus < or = 6 units) was observed, from an initially insignificantly increased relative hazard (RH) (RH = 1.44 at 6 months) to an insignificantly decreased effect after longer follow-up (RH = 0.53 after 2 years).CONCLUSION:Although no overall association between blood transfusion and cancer-related mortality was found, a tendency towards an increased risk early in the follow-up period was observed if more than 6 units were transfused. However, these results need confirmation in further studies before a restrictive attitude towards peri-operative blood transfusion is recommended.
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| 7. |
- Jonsson, Maria, et al.
(författare)
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Heterogeneity of outer membrane proteins in Borrelia burgdorferi : comparison of osp operons of three isolates of different geographic origins.
- 1992
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Ingår i: Infection and Immunity. - : American Society for Microbiology. - 0019-9567 .- 1098-5522. ; 60:5, s. 1845-53
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Tidskriftsartikel (refereegranskat)abstract
- Biochemical and immunochemical studies of the outer membrane proteins of Borrelia burgdorferi have shown that the OspA and OspB proteins from strains of different geographic origins may differ considerably in their reactivities with monoclonal antibodies and in their apparent molecular weights. To further characterize this variation in Osp proteins between strains, the osp operons and deduced translation products from two strains, one from Sweden (ACAI) and one from eastern Russia (Ip90), were studied. Polyacrylamide gel electrophoresis and Western blot (immunoblot) analyses confirmed differences between ACAI, Ip90, and the North American strain B31 in their Osp proteins. The sequences of the ospA and ospB genes of ACAI and Ip90 were compared with that of the previously studied osp operon of B31 (S. Bergström, V. G. Bundoc, and A. G. Barbour, Mol. Microbiol. 3:479-486, 1989). The osp genes of ACAI and Ip90, like the corresponding genes of B31, were found on plasmids with apparent sizes of about 50 kb and are cotranscribed as a single unit. Pairwise comparisons of the nucleotide sequences revealed that the ospA genes of ACAI and Ip90 were 85 and 86% identical, respectively, to the ospA gene of strain B31 and 86% identical to each other. The ospB sequences of these two strains were 79% identical to the ospB gene of B31 and 81% identical to each other. There was significantly greater similarity between the ospA genes of the three different strains than there was between the ospA and ospB genes within each strain. These studies suggest that the duplication of osp genes in B. burgdorferi occurred before the geographical dispersion of strains represented by ACAI, Ip90, and B31.
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| 8. |
- Lönnerdal, Bo, et al.
(författare)
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Cloning and sequencing of a cDNA encoding human milk beta-casein.
- 1990
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Ingår i: FEBS Letters. - 0014-5793 .- 1873-3468. ; 269:1, s. 153-6
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Tidskriftsartikel (refereegranskat)abstract
- A cDNA of 1065 bp encoding the human milk beta-casein was cloned and sequenced using a synthetic oligodeoxyribonucleotide probe and a human mammary gland library. The nucleotide (nt) sequence contained an open reading frame sufficient to encode the entire amino-acid (aa) sequence of a beta-casein precursor protein consisting of 210 aa and a signal peptide of 15 aa. The nt sequence shows 45-62% homology to those of bovine, ovine, rat, and mouse beta-caseins. The highly phosphorylated site, which is responsible for the calcium-binding capacity of beta-casein, the signal peptide, and a sequence encoding for an inhibitor to the angiotensin-converting enzyme seem highly conserved among the beta-caseins with known sequences.
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| 9. |
- Sandström, Herbert, et al.
(författare)
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Intravascular haemolysis and increased prevalence of myeloma and monoclonal gammopathy in congenital dyserythropoietic anaemia, type III.
- 1994
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Ingår i: European Journal of Haematology. - 0902-4441 .- 1600-0609. ; 52:1, s. 42-6
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Tidskriftsartikel (refereegranskat)abstract
- A family with congenital dyserythropoietic anaemia type III was studied. Twenty patients and 10 of their healthy siblings were clinically examined and questioned about their medical history. Blood sampling and bone marrow aspirations were also performed. Forty-five percent of the patients reported symptoms of anaemia and 35% regularly felt weakness, fatigue, or headache. However, the majority of the patients regarded themselves as healthy. The bone marrow showed a uniform picture of erythroid hyperplasia with multinuclear erythroblasts and gigantoblasts with up to 12 nuclei. There was laboratory evidence of intravascular haemolysis and mild anaemia. We also observed a high prevalence of monoclonal gammopathy of undetermined significance (3 cases) and myeloma (1 case) among the patients.
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| 10. |
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| 11. |
- Thoma, s V, et al.
(författare)
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Hydrophilized and functionalized microtiter plates for the site-specific coupling of antigens and antibodies: application to the diagnosis of viral cardiac and autoimmune diseases
- 1993
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Ingår i: Colloids and Surfaces A. - 0927-7757 .- 1873-4359. ; 77, s. 125-139
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Tidskriftsartikel (refereegranskat)abstract
- Extensive adsorption of macromolecules (antigens and antibodies) took place when they were covalently bound to non-hydrophilized polystyrene microtiter plates. Precoating polystyrene surfaces with gelatin reduced this non-specific adsorption only partially, whereas precoating with uncharged polymers such as poly(ethylene glycol) (PEG) and polysaccharides eliminated the problem totally. On such hydrophilized plates functionalized with epoxide groups, antigens and antibodies were randomly bound. On those functionalized with acid hydrazide, antibodies were site-specifically bound by their carbohydrate residues. These site-specifically bound antibodies retained greater reactivity and more of their native antigenic structure than randomly coupled antibodies. They therefore permitted the measurement of a minor analyte (D-dimer) in the presence of an excess of major components such as fibrinogen. Enzyme immunoassays which were uninterpretable on non-hydrophilized plates because Or the adsorption Or immunoglobulin gave meaningful results on hydrophilized plates. This held true for immunoglobulin aggregates formed artificially either by successive cycles of freezing and thawing or by the acid treatment of serum to dissociate immune complexes. The latter approach permitted us to obtain unequivocal evidence for the presence of antibodies specific for human T-cell Iymphotropic virus type I (HTLV1) in immune complexes in sera with no detectable free antibody. For naturally occurring aggregates which are often found in the sera of patients with autoimmune diseases, the use of hydrophilized plates also permitted antibody levels to be measured in instances where background noise was greater than the signal on non-hydrophilized plates. This combination of hydrophilization and site-specific coupling of monoclonal antibodies of a defined specificity should provide a distinct advantage when developing routine covalent enzyme linked immunoassays (CELIAs) for minor analytes, in a mixture with major constituents, as is often encountered, in both human and veterinary medicine and in the food industry.
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| 12. |
- Wassgren, Ann Britt, et al.
(författare)
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Pheromone related compounds in pupal and adult female pine sawflies, Neodiprion sertifer, of different age and in different parts of the body
- 1992
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Ingår i: Journal of Insect Physiology. - 0022-1910. ; 38:11, s. 885-893
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Tidskriftsartikel (refereegranskat)abstract
- The pine sawfly pheromone precursor 3,7-dimethyl-2-pentadecanol (diprionol) was quantified by gas chromatography in different body parts of virgin female Neodiprion sertifer. About one-third of the total amount (approx. 10 ng per female) was found in each of head + thorax, abdominal segments 1-3, and the remaining abdomen. Diprionol was also found in the respective parts of pupae, but in lower amounts. This suggests that at least the final steps of the pheromone biosynthesis take place in many parts of the body, possibly in the cuticle. The diprionol content remained relatively constant over the whole lifetime of virgin females. By using coupled gas chromatography-electroantennogram detection three active compounds in the acetylated female extract were observed. These corresponded to the pheromone, (2S,3S,7S)-diprionyl acetate, and its homologues with the chain shortened by one and two carbons respectively, as determined by gas chromatography-mass spectrometry. In addition, the homologue having a chain elongated by one carbon was found. The homologues occurred in amounts of 2-5% of the diprionyl acetate. When synthetic homologues were tested in the field, alone or in combination with diprionyl acetate, they were not behaviourally active to male sawflies, despite their electroantennogram activity. A threo-isomer of diprionol, most likely (2S,3R,7R), was present in the females in amounts less than 0.5% of the (2S,3S,7S)-content. The acetate of this threo-isomer is known as both synergist and inhibitor to the (2S,3S,7S)-isomer. trans-Perillenal was found in the abdominal segments 1-3 of both males and females and its possible biosynthetic relationship to diprionol is discussed.
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