| 1. |
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| 2. |
- Thörn, Ingrid, 1957-, et al.
(author)
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Minimal residual disease assessment in childhood acute lymphoblastic leukemia : Results of a Swedish multi-centre study comparing real-time PCR and multi-colour flow cytometry
- 2009
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Other publication (other academic/artistic)abstract
- In this Swedish multi-center study of early treatment response in childhood acute lymphoblastic leukemia (ALL), we evaluated the concordance between multicolour flow cytometry (FCM) and real-time quantitative polymerase chain reaction (RQ-PCR) for assessment of minimal residual disease (MRD). Multiple time points (i.e. day 15, 29, 50 and 106) were evaluated with the NOPHO (Nordic Society of Pediatric Hematology and Oncology) ALL 2000 treatment protocol as backbone. During 2002-2006, 334 children were diagnosed with ALL, where 228 had paired samples taken at any of the four time points. With the detection level of 0.1%, the concordance between RQ-PCR and FCM was 90% in the 726 paired samples analyzed. At day 29, the correlation between the methods was greater with MRD levels >0.1% (rs=0.7, p<0.001) than below (rs=0.2, p=0.024). MRD levels higher than 0.1% at day 29 was a significant predictor of higher risk of having a bone marrow relapse. This was true both for BCP ALL and T-ALL analysed with either FCM or RQ-PCR, although RQ-PCR was a better discriminator than FCM in T-ALL. However, using the NOPHO ALL 2000 protocol, our data indicate that a higher cut-off value (0.2%) should be applied in BCP ALL when using RQ-PCR as MRD method. In contrast, MRD levels ≥ 0.1%, analysed with either method late during induction therapy, was not a predictor of isolated extramedullary relapse. We therefore conclude that MRD assessment by RQ-PCR based IG/TCR rearrangement and multicolour FCM monitoring can be used as a clinical tool if the aim is to find childhood ALL cases with increased risk of having bone marrow relapses.
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| 3. |
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| 4. |
- Andersson, Sören, et al.
(author)
-
Frictions models for sliding dry, boundary and mixed lubricated contacts
- 2007
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In: Tribology International. - : Elsevier BV. - 0301-679X .- 1879-2464. ; 40, s. 580-587
-
Journal article (peer-reviewed)abstract
- Friction, lubrication, and wear have a strong influence on the performance and behavior of mechanical systems. This paper deals with different friction models for sliding contacts running under different conditions. The models presented are suited to different situations, depending on the type of contact, running conditions, and the behavior of interest. The models will be discussed from simulation and tribological points of view. The different types of friction models considered are:center dot friction models for transient sliding under dry, boundary and mixed lubrication conditions,center dot friction models for micro-displacements of engineering surfaces subjected to transient sliding,center dot friction models often used in the simulation and control of technical systems,center dot combined friction models that represent physical behaviors fairly well but are also suitable for use in simulating systems,center dot friction models that take into account the stochastic nature of interacting surface asperities
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| 5. |
- Balciunas, Darius, et al.
(author)
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Functional interactions within yeast mediator and evidence of differential subunit modifications
- 2003
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In: Journal of Biological Chemistry. - 0021-9258 .- 1083-351X. ; 278:6, s. 3831-3839
-
Journal article (peer-reviewed)abstract
- It is possible to recruit RNA polymerase II to a target promoter and, thus, activate transcription by fusing Mediator subunits to a DNA binding domain. To investigate functional interactions within Mediator, we have tested such fusions of the lexA DNA binding domain to Med1, Med2, Gal11, Srb7, and Srb10 in wild type, med1, med2, gal11, sin4, srb8, srb10, and srb11 strains. We found that lexA-Med2 and lexA-Gal11 are strong activators that are independent of all Mediator subunits tested. lexA-Srb10 is a weak activator that depends on Srb8 and Srb11. lexA-Med1 and lexA-Srb7 are both cryptic activators that become active in the absence of Srb8, Srb10, Srb11, or Sin4. An unexpected finding was that lexA-VP16 differs from Gal4-VP16 in that it is independent of the activator binding Mediator module. Both lexA-Med1 and lexA-Srb7 are stably associated with Med4 and Med8, which suggests that they are incorporated into Mediator. Med4 and Med8 exist in two mobility forms that differ in their association with lexA-Med1 and lexA-Srb7. Within purified Mediator, Med4 is present as a phosphorylated lower mobility form. Taken together, these results suggest that assembly of Mediator is a multistep process that involves conversion of both Med4 and Med8 to their low mobility forms.
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| 6. |
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| 7. |
- Björklund, Elisabet, et al.
(author)
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Quality control of flow cytometry data analysis for evaluation of minimal residual disease in bone marrow from acute leukemia patients during treatment.
- 2009
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In: Journal of pediatric hematology/oncology : official journal of the American Society of Pediatric Hematology/Oncology. - : Lippincott Williams & Wilkins. - 1536-3678 .- 1077-4114. ; 31:6, s. 406-15
-
Journal article (peer-reviewed)abstract
- Low levels of leukemia cells in the bone marrow, minimal residual disease (MRD), are considered to be a powerful indicator of treatment response in acute lymphatic leukemia (ALL). A Nordic quality assurance program, aimed on standardization of the flow cytometry MRD analysis, has been established before implementation of MRD at cutoff level 10 as one of stratifying parameters in next Nordic Society of Pediatric Hematology and Oncology (NOPHO) treatment program for ALL. In 4 quality control (QC) rounds 15 laboratories determined the MRD levels in 48 follow-up samples from 12 ALL patients treated according to NOPHO 2000. Analysis procedures were standardized. For each QC round a compact disc containing data in list-mode files was sent out and results were submitted to a central laboratory. At cutoff level 10, which will be applied for clinical decisions, laboratories obtained a high concordance (91.6%). If cutoff level 10 was applied, the concordance would be lower (85.3%). The continuing standardization resulted in better concordance in QC3 and QC4 compared with QC1 and QC2. The concordance was higher in precursor B as compared with T-cell ALL. We conclude that after standardization, flow cytometry MRD detection can be reliably applied in international, multicenter treatment protocols.
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| 8. |
- Björklund, Stefan, et al.
(author)
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Compensation of systematic errors in five-axis high-speed machining
- 2002
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In: International Journal of Production Research. - : Informa UK Limited. - 0020-7543 .- 1366-588X. ; 40:15 SPEC., s. 3765-3778
-
Journal article (peer-reviewed)abstract
- A method is described for the compensation of errors associated with tool path generation, particularly during five-axis high-speed machining (HSM). Information on machine tool performance and its dynamic features is used to calculate possible errors and convenient modifications of the NC program, thereby avoiding errors when parts are actually being machined. This 'preprocess method' by means of postprocessing with NC software is presented. The errors dealt with are mainly servo lag errors, but the explored approach can support most systematic errors associated with machine tool performance. These are briefly summarized. Research so far has largely been aimed at the implementation of compensation routines in the CNC controller and at corrections in real time. The problem is that most applications are only available for three-axis milling. The presented approach (compensation before machining by using software routines in a specially designed postprocessor) is based on a fuzzy logic expert system. The benefits can be summarized as data reduction, data improvement, precontrol of feed, and improved component accuracy. The applied procedure is also convenient for implementation in an industrial environment by retrofitting existing equipment. The suggested method provides improved control over machine dynamics, permitting high-speed machining centres to maintain a maximum or near-maximum feed rate despite axis reversals and tool path changes, even at corners. Two categories of results are presented, namely the management of NC data related to expected performance of the machine tool and improvements of the machine tool performance in terms of productivity and accuracy of the machined test components.
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| 9. |
- Björklund, Stefan
(author)
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Reformismens anatomi : Erasmus versus Luther
- 2009
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Book (other academic/artistic)abstract
- The author analyses the sharp philosophical debates between Erasmus Rotterdamus and Martin Luther, revolutionary and determinist, and takes that as a ground for discussion of the concept of "reformism". Ha proposes that "reformism" should [sic] used for a "step-by-step" method of political change, irrespective of the content of policies. He also tries to list some characteristics of the "reformistic" attitude.
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| 10. |
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| 11. |
- Björklund, Stefan
(author)
-
The influence of surface roughness in elliptical contacts
- 2001
-
In: Tribology International. - 0301-679X .- 1879-2464. ; 34:12, s. 841-845
-
Journal article (peer-reviewed)abstract
- The influence of surface roughness on contact behaviour is of great importance in many tribological situations. In the last decade several methods to calculate the pressure distribution and the real contact area in contacts between rough surfaces have been described. A problem arising for slender elliptical contacts, such as between gear teeth, roller and raceway, cam and follower, etc., is that the size of the contact is much greater than the size of the asperities. Accordingly the number of contact nodes necessary for an accurate solution to the problem becomes excessively large. This paper describes a method to calculate the influence of three-dimensional surface roughness in contacts that are very long in one direction. The method is based on restricting the calculations to a subpart of the real contact area, while the rest of the contact is taken into account by mirroring techniques. The results show that the real contact area is very sensitive to the amplitude of the roughness, while the waviness is less important. An equation is suggested from which the real area can be calculated if the smooth case contact parameters and two roughness parameters are known.
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| 12. |
- Björklund, Stefan, et al.
(author)
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The mediator complex
- 2004
-
In: Advances in protein chemistry. - 0065-3233 .- 1557-8941. ; 67, s. 43-65
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Journal article (peer-reviewed)
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| 13. |
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| 14. |
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| 15. |
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| 16. |
- Choquet, Isabelle, 1965-, et al.
(author)
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Clogging and lump formation during atmospheric plasma spraying with powder injection downstream the plasma gun
- 2007
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In: Journal of thermal spray technology (Print). - : Springer Science and Business Media LLC. - 1059-9630 .- 1544-1016. ; 16:4, s. 512-523
-
Journal article (peer-reviewed)abstract
- This study aimed to numerically and experimentally investigate lump formation during atmospheric plasma spraying with powder injection downstream the plasma gun exit. A first set of investigations was focused on the location and orientation of the powder port injector. It turned out impossible to keep the coating quality while avoiding lumps by simply moving the powder injector. A new geometry of the powder port ring holder was designed and optimized to prevent nozzle clogging, and lump formation using a gas screen. This solution was successfully tested for applications with Ni-5wt.%Al and ZrO2-7wt.%Y2O3 powders used in production. The possible secondary effect of plasma jet shrouding by the gas screen, and its consequence on powder particles prior to impact was also studied.
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| 17. |
- Choquet, Isabelle, 1965-, et al.
(author)
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New powder port holder geometry to avoid lump formation in APS
- 2005
-
In: 17th international symposium on plasma chemistry (ISPC 17). ; , s. OP7. 1-6
-
Conference paper (other academic/artistic)abstract
- A new geometry of the powder port ring holder used in atmospheric plasma spraying has recently been designed to avoid lump formation, and successfully tested for a set of process parameters associated with Ni-5Al powder used in production to form bond coat [1]. But with ZrO 2 powder used to made top coat, improvements were not enough satisfactory. Here, we investigate numerically the cause of the remaining defects, and further improve the ring geometry to prevent lump from forming in any part of the coating.
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| 18. |
- Flanagan, John N., et al.
(author)
-
Role of follistatin in promoting adipogenesis in women
- 2009
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In: Journal of Clinical Endocrinology and Metabolism. - : The Endocrine Society. - 0021-972X .- 1945-7197. ; 94:8, s. 3003-9
-
Journal article (peer-reviewed)abstract
- CONTEXT: Follistatin is a glycoprotein that binds and neutralizes biological activities of TGFbeta superfamily members including activin and myostatin. We previously identified by expression profiling that follistatin levels in white adipose tissue (WAT) were regulated by obesity. OBJECTIVE: The objective of the study was to elucidate the role of follistatin in human WAT and obesity. DESIGN: We measured secreted follistatin protein from WAT biopsies and fat cells in vitro. We also quantified follistatin mRNA expression in sc and visceral WAT and in WAT-fractionated cells and related it to obesity status, body region, and cellular origin. We investigated the effects of follistatin on adipocyte differentiation of progenitor cells in vitro. PARTICIPANTS: Women (n = 66) with a wide variation in body mass index were recruited by advertisement and from a clinic for weight-reduction therapy. RESULTS: WAT secreted follistatin in vitro. Follistatin mRNA levels in sc but not visceral WAT were decreased in obesity and restored to nonobese levels after weight reduction. Follistatin mRNA levels were high in the stroma-vascular fraction of WAT and low in adipocytes. Recombinant follistatin treatment promoted adipogenic differentiation of progenitor cells and neutralized the inhibitory action of myostatin on differentiation in vitro. Moreover, activin and myostatin signaling receptors were detected in WAT and adipocytes. CONCLUSION: Follistatin is a new adipokine important for adipogenesis. Down-regulated WAT expression of follistatin in obesity may counteract adiposity but could, by inhibiting adipogenesis, contribute to hypertrophic obesity (large fat cells) and insulin resistance.
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| 19. |
- Frodelius, Jenny, et al.
(author)
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Ti2AlC coatings deposited by High Velocity Oxy-Fuel spraying
- 2008
-
In: Surface & Coatings Technology. - : Elsevier. - 0257-8972 .- 1879-3347. ; 202:24, s. 5976-5981
-
Journal article (peer-reviewed)abstract
- High Velocity Oxy-Fuel has been utilized to spray coatings from Ti2AlC (MAXTHAL 211®) powders. X-ray diffraction showed that the coatings consist predominantly of Ti2AlC with inclusions of the phases Ti3AlC2, TiC, and Al–Ti alloys. The fraction of Ti2AlC in coatings sprayed with a powder size of 38 μm was found to increase with decreasing power of the spraying flame as controlled by the total gas flow of H2 and O2. A more coarse powder (56 μm) is less sensitive to the total gas flow and retains higher volume fraction of MAX-phase in the coatings, however, at the expense of increasing porosity. X-ray pole figure measurements showed a preferred crystal orientation in the coatings with the Ti2AlC (000l) planes aligned to the substrate surface. Bending tests show a good adhesion to stainless steel substrates and indentation yields a hardness of 3–5 GPa for the coatings sprayed with a powder size of 38 μm.
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| 20. |
- Hallberg, Magnus, et al.
(author)
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Functional and physical interactions within the middle domain of the yeast mediator
- 2006
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In: Molecular Genetics and Genomics. - : Springer Science and Business Media LLC. - 1617-4615 .- 1617-4623. ; 276:2, s. 197-210
-
Journal article (peer-reviewed)abstract
- Med21 (Srb7) is a small essential subunit of the middle domain of the Mediator, which is conserved in all eukaryotes. It is thought to play an important role in both transcriptional activation and repression. In the yeast Saccharomyces cerevisiae, Med21 is known to interact both with the Mediator subunit Med6 and the global co-repressor Tup1. We have made a temperature-sensitive med21-ts mutant, which we used in a high copy number suppressor screen. We found ten yeast genes that can suppress the med21-ts mutation in high copy number. The three strongest suppressors were MED7 and MED10 (NUT2), which encode other Mediator subunits, and ASH1, which encodes a repressor of the HO gene. 2-Hybrid experiments confirmed multiple interactions between Med21, Med10, Med7 and Med4, and also revealed a Med21 self-interaction. The interactions of Med21 with Med7 and Med10 were verified by co-immunoprecipitation of tagged proteins produced in insect cells and E. coli, where both interactions were found to depend strongly on the amino acid residues 2-8 of Med21. These interactions, and the interactions of Med21 with Med6 and Tup1, suggest that Med21 may serve as a molecular switchboard that integrates different signals before they reach the core polymerase.
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| 21. |
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| 22. |
- Hallberg, Magnus, 1974-
(author)
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Studies of Functional Interactions within Yeast Mediator and a Proposed Novel Mechanism for Regulation of Gene Expression
- 2004
-
Doctoral thesis (other academic/artistic)abstract
- The yeast Mediator complex is required for transcriptional regulation both in vivo and in vitro and the identification of similar complexes from metazoans indicates that its function is conserved through evolution. Mediator subunit composition and structure is well characterized both by biochemical, genetic and biophysical methods. In contrast, little is known about the mechanisms by which Mediator operates and how the complex is regulated. The aim of my thesis was to elucidate how Mediator functions at the molecular level and to investigate functional interactions within Mediator. It is possible to recruit RNA polymerase II to a target promoter and thus to activate transcription by fusing Mediator subunits to a DNA binding domain. In order to investigate functional interactions within Mediator, we made such fusion proteins where different Mediator subunits were fused to the DNA binding domain of lexA. The expression of a reporter gene containing binding sites for lexA was subsequently measured in both a wild type strain and in strains where genes encoding specific Mediator subunits had been disrupted. We found that lexA-Med2 and lexA-Gal11 are strong activators that function independently of all Mediator subunits tested. On the other hand, lexA-Srb10 is a weak activator that depends on Srb8 and Srb11 and lexA-Med1 and lexA-Srb7 are both cryptic activators that become active in the absence of Srb8, Srb10, Srb11, or Sin4. Both lexA-Med1 and lexA-Srb7 proteins showed a stable association with the Mediator subunits Med4 and Med8 in wild type cells and in all deletion strains tested, indicating that they were functionally incorporated into the Mediator complex. We also showed that both Med4 and Med8 exist in two forms that differed in electrophoretic mobility and that these forms differed in their ability to associate with Mediator immuno-purified from the LEXA-SRB7 and LEXA-MED1 strains. Dephosphorylation assays of purified Mediator indicated that the two mobility forms of Med4 corresponded to the phosphorylated and unphosphorylated forms of the Med4 protein respectively. Some of the data presented in this study as well as previous genetic and biochemical data obtained in our lab suggested a functional link between the Med1, Med2, Srb10 and Srb11 proteins. We extended these findings by showing that the Srb10 kinase phosphorylates the Med2 protein at residue serine 208, both in vitro and in vivo. We also showed that a point mutation of the single phosphorylation site to an alanine or to an aspartic acid residue altered the gene expression of a specific set of genes. Taken together, these data indicate that posttranslational modification of Mediator subunits is a so far uncharacterized mechanism for regulation of gene expression. In order to study the function of the Srb7 subunit of Mediator, we isolated a temperature sensitive strain where the amino acids 2 to 8 of srb7 were deleted. The Mediator subunits Nut2 and Med7 were isolated as high copy suppressor of srb7-∆(2-8) and we were also able to show that Srb7 interacted with Nut2 and Med7 both in a 2-hybrid system and in co-immuno precipitation experiments using recombinantly expressed proteins. Interestingly, a deletion of amino acids 2 to 8 of Srb7 abolishes its interaction with both Med7 and Nut2 in vitro. Med4 also interacted with Srb7 in the 2-hybrid system and surprisingly, the first eight amino acids of Srb7 were shown to be sufficient for this interaction.
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| 23. |
- Holmberg, Hans-Christer, et al.
(author)
-
Contribution of the legs to double-poling performance in elite cross-country skiers.
- 2006
-
In: Medicine and science in sports and exercise. - : Ovid Technologies (Wolters Kluwer Health). - 0195-9131 .- 1530-0315. ; 38:10, s. 1853-60
-
Journal article (peer-reviewed)abstract
- In the classical style of cross-country skiing, the double-poling (DP) technique, which is regarded as an upper-body exercise, is used on the flatter parts of a course. Limited biomechanical and physiological data are available about DP compared with other cross-country skiing techniques. The purpose of the present study was to evaluate the possible role of the lower body during DP.Eleven elite cross-country skiers performed two incremental tests using DP roller skiing at 1 degree inclination on a treadmill with or without locking the knee and ankle joints (DPLOCKED and DPFREE). Maximal and peak oxygen uptake (VO2max and VO2peak) during classic diagonal skiing and DP, respectively, were measured. In addition, heart rate, blood lactate concentration, and maximal DP velocity (Vmax) were determined. Pole-ground reaction forces and joint angles (elbow, hip, knee, and ankle) were analyzed.The skiers obtained 7.7% higher VO2peak, 9.4% higher Vmax, and 11.7% longer time to exhaustion during DPFREE compared with DPLOCKED (all P < 0.05). There was a higher heart rate and blood lactate concentration in DPLOCKED at submaximal stages (all P < 0.05), with no difference in oxygen consumption. At 85% Vmax, corresponding to approximately 81% VO2peak FREE, the differences in physiological variables were accompanied by a 13.6% higher poling frequency, a 4.9% shorter poling phase, 13.3% shorter recovery phase, and 10.9% lower relative pole force in DPLOCKED (all P < 0.05).Movements of the knee and ankle joints are an integrative part in the skillful use of the DP technique, and restriction of the motion in these joints markedly affects both biomechanical and physiological variables, impairing DP performance.
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| 24. |
- Iwnicki, Simon, et al.
(author)
-
Wheel-rail contact mechanics
- 2009
-
In: Wheel-rail interface handbook. - Cambridge, UK : Woodhead Publishing Ltd. - 9781845694128 - 9781845696788 - 9781439801468 ; , s. 58-92
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Book chapter (peer-reviewed)
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| 25. |
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| 26. |
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| 27. |
- Kotova, Irina, et al.
(author)
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A mouse in vitro transcription system reconstituted from highly purified RNA polymerase II, TFIIH, and recombinant TBP, TFIIB, TFIIE and TFIIF.
- 2001
-
In: European Journal of Biochemistry. - : Wiley. - 0014-2956 .- 1432-1033. ; 268:16, s. 4527-4536
-
Journal article (peer-reviewed)abstract
- Unregulated transcription of protein-encoding genes in vitro is dependent on 12-subunit core RNA polymerase II and five general transcription factors; TATA binding protein (TBP), transcription factor (TF)IIB, TFIIE, TFIIF, and TFIIH. Here we describe cloning of the mouse cDNAs encoding TFIIB and the small and large TFIIE and TFIIF subunits. The cDNAs have been used to express the corresponding proteins in recombinant form in Escherichia coli and in Sf21 insect cells, and all proteins have been purified to > 90% homogeneity. We have also purified a recombinant His6-tagged mouse TBP to near homogeneity and show that it is active in both a reconstituted mouse in vitro transcription system and a TBP-dependent in vitro transcription system from Saccharomyces cerevisiae. The more complex general transcription factors, TFIIH and RNA polymerase II, were purified more than 1000-fold and to near homogeneity, respectively, from tissue cultured mouse cells. When combined, the purified factors were sufficient to initiate transcription from different promoters in vitro. Functional studies of the S-phase-specific mouse ribonucleotide reductase R2 promoter using both the highly purified system described here (a mouse cell nuclear extract in vitro transcription system) and in vivo R2-promoter reporter gene assays together identify an NF-Y interacting promoter proximal CCAAT-box as being essential for high-level expression from the R2 promoter.
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| 28. |
- Kotova, Irina, et al.
(author)
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Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription
- 2003
-
In: European Journal of Biochemistry. - : Blackwell Publishing. - 0014-2956 .- 1432-1033. ; 270:8, s. 1791-1801
-
Journal article (peer-reviewed)abstract
- Ribonucleotide reductase is essential for the synthesis of all four dNTPs required for DNA replication. The enzyme is composed of two proteins, R1 and R2, which are both needed for activity. Expression of the R1 and R2 mRNAs is restricted to the S-phase of the cell cycle, but the R1 and R2 promoters show no obvious sequence homologies that could indicate coordination of transcription. Here we study initiation of transcription at the natural mouse R2 promoter, which contains an atypical TATA-box with the sequence TTTAAA, using a combination of in vivo reporter gene assays and in vitro transcription. Our results indicate that in constructs where sequences from the R2 5'-UTR are present, the mouse R2 TATA-box is dispensable both for unregulated, basal transcription from the R2 promoter and for S-phase specific activity. Instead, initiation of R2 transcription is directed by sequences downstream from the transcription start. We report that this region contains a conserved palindrome sequence that interacts with TAF(II)s. This interaction down-regulates basal transcription from the R2 promoter, both in the absence and in the presence of the TATA-box.
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| 29. |
- Lenner, Matz, 1944-, et al.
(author)
-
High Speed Machining
- 2001
-
In: The 5th Intl Conference on Modern Technologies in Manufacturing,2001.
-
Conference paper (other academic/artistic)
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| 30. |
- Lindholm, Per, et al.
(author)
-
Characterization of wear on a cam follower system in a diesel engine
- 2003
-
In: Wear 254. - : Elsevier. ; , s. 1199-1207
-
Journal article (peer-reviewed)abstract
- This paper presents an investigation of the running in of the most important contact surfaces of a modern diesel cam follower system. The test equipment used consists of a commercially available cylinder head with an overhead camshaft and valve train system for six cylinders. The load on the contacting surfaces is varied by controlling the fuel injector pumps. The running in is investigated by analysing the changes in topography of the roller, pin and rocker arm of the fuel injector arm. Seven test series were conducted for 1, 10 and 100 h with a variation of the load and speed between a high and low level. The test time was not long enough to be able to see any changes in the surface topography of the roller or pin surfaces. However the wear on the roller bearing surface and the rocker arm bearing surface was significant. Here the surface peak heights are worn off and the surfaces are smoothed out. The observed changes in surface topography are related to the current lubrication regime and the wear is discussed in terms of the λ -value.
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| 31. |
- Lindholm, Per, et al.
(author)
-
Contact conditions in a cam and roller contact
- 2001
-
In: Proceedings of World Tribology Congress WT2001. - Wien : Österreichische Tribologishe Gesellshaft. - 390165707X
-
Conference paper (other academic/artistic)
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| 32. |
- Lindholm, Per, et al.
(author)
-
Method and surface roughness aspects for the design of DLC coatings
- 2006
-
In: Wear. - : Elsevier BV. - 0043-1648 .- 1873-2577. ; 261:1, s. 107-111
-
Journal article (peer-reviewed)abstract
- The design of coatings for highly loaded component contacts, such as bearings, gears and valve train components involves several important factors, including load, friction, lubrication, surface characteristics and material parameters. This paper presents an investigation of the influence of the material, coating thickness and surface roughness on tensional stress levels for coatings that are more compliant than the substrate material. Specifically the effect of multiple asperity contact is studied in three dimensions. The simulation is based on a finite element model where the load is applied as several interacting Hertzian pressure distributions. The results show that the surface structure, in combination with the elastic properties of the coating, has a large influence on the tensional stress level in the coating. The highest tensional stress level in the coating occurs when contact spots almost overlap neighbouring cells and at the same time the size of the contact spots is in the same order of magnitude as the coating thickness.
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| 33. |
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| 34. |
- Marshall, M. B., et al.
(author)
-
Experimental characterization of wheel-rail contact patch evolution
- 2006
-
In: Journal of tribology. - : ASME International. - 0742-4787 .- 1528-8897. ; 128:3, s. 493-504
-
Journal article (peer-reviewed)abstract
- The contact area and pressure distribution in a wheel/rail contact is essential information required in any fatigue or wear calculations to determine design life, re-grinding, and maintenance schedules. As wheel or rail wear or surface damage takes place the contact patch size and shape will change. This leads to a redistribution of the contact stresses. The aim of this work was to use ultrasound to nondestructively quantify the stress distribution in new, worn, and damaged wheel-rail contacts. The response of a wheel/rail interface to an ultrasonic wave can be modeled as a spring. If the contact pressure is high the interface is very stiff, with few air gaps, and allows the transmission of an ultrasonic sound wave. If the pressure is low, interfacial stiffness is lower and almost all the ultrasound is reflected. A quasistatic spring model was used to determine maps of contact stiffness from wheel/rail ultrasonic reflection data. Pressure was then determined using a parallel calibration experiment. Three different contacts were investigated; those resulting from unused, worn, and sand damaged wheel and rail specimens. Measured contact pressure distributions are compared to those determined using elastic analytical and numerical elastic-plastic solutions. Unused as-machined contact surfaces had similar contact areas to predicted elastic Hertzian solutions. However, within the contact patch, the numerical models better reproduced the stress distribution, as they incorporated real surface roughness effects. The worn surfaces were smoother and more conformal, resulting in a larger contact patch and lower contact stress. Sand damaged surfaces were extremely rough and resulted in highly fragmented contact regions and high local contact stress.
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| 35. |
- Marshall, M. B., et al.
(author)
-
Ultrasonic characterisation of a wheel/rail contact
- 2004
-
In: Transient Processes in Tribology. - : Elsevier. - 0444517065 ; , s. 151-158
-
Conference paper (peer-reviewed)abstract
- A quantification of stress in a wheel/rail contact is essential information required in fatigue and wear calculations for determining design life, regrinding and maintenance schedules. The aim of this work was to use ultrasound to non-destructively determine wheel/rail contact pressures. A wheel/rail interface behaves like a spring. If the pressure is high there are few air gaps; so it is very stiff and allows transmission of an ultrasonic wave. If the pressure is low then interface stiffness is lower and most ultrasound is reflected. A spring model was used to determine maps of contact stiffness from wheel/rail contact ultrasonic reflection data. A calibration procedure was then used to determine the pressure. Measured contact pressure contours are compared with those predicted by various contact theories.
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| 36. |
- Nygren, Jens Martin, 1976-, et al.
(author)
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Myeloid and lymphoid contribution to non-haematopoietic lineages through irradiation-induced heterotypic cell fusion
- 2008
-
In: Nature Cell Biology. - London : Nature Publishing Group. - 1465-7392 .- 1476-4679. ; 10:5, s. 584-92
-
Journal article (peer-reviewed)abstract
- Recent studies have suggested that regeneration of non-haematopoietic cell lineages can occur through heterotypic cell fusion with haematopoietic cells of the myeloid lineage. Here we show that lymphocytes also form heterotypic-fusion hybrids with cardiomyocytes, skeletal muscle, hepatocytes and Purkinje neurons. However, through lineage fate-mapping we demonstrate that such in vivo fusion of lymphoid and myeloid blood cells does not occur to an appreciable extent in steady-state adult tissues or during normal development. Rather, fusion of blood cells with different non-haematopoietic cell types is induced by organ-specific injuries or whole-body irradiation, which has been used in previous studies to condition recipients of bone marrow transplants. Our findings demonstrate that blood cells of the lymphoid and myeloid lineages contribute to various non-haematopoietic tissues by forming rare fusion hybrids, but almost exclusively in response to injuries or inflammation.
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| 37. |
- Olofsson, Ulf, et al.
(author)
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Simulation of mild wear in boundary lubricated spherical roller thrust bearings
- 2000
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In: Wear. - 0043-1648 .- 1873-2577. ; 241:2, s. 180-185
-
Journal article (peer-reviewed)abstract
- Owing to the curved contact surfaces in a spherical roller thrust beating, the rollers will undergo sliding. For an unskewed roller there will be two points along each contact where the sliding velocity is zero. At all other points along the contact, sliding is present. Under boundary lubricated conditions the sliding can give rise to mild wear. Experimental results show that this wear can cause a significant change in the surface profile outside the zero sliding points. The mild wear in the contact was simulated using Archard's wear law. An iterative wear model is described in which the normal load distribution, the tangential tractions and the sliding distances are repeatedly calculated to simulate the changes in surface geometry due to wear. Good qualitative agreement was achieved between the simulation results and the previously presented experimental results.
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| 38. |
- Sellgren, U., et al.
(author)
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A finite element-based model of normal contact between rough surfaces
- 2003
-
In: Wear. - 0043-1648 .- 1873-2577. ; 254:11, s. 1180-1188
-
Journal article (peer-reviewed)abstract
- Engineering surfaces can be characterized as more or less randomly rough. Contact between engineering surfaces is thus discontinuous and the real area of contact is a small fraction of the nominal contact area. The stiffness of a rough surface layer thus influences the contact state as well as the behavior of the surrounding system. A contact model that takes the properties of engineering surfaces into account has been developed and implemented using finite element software. The results obtained with the model have been verified by comparison with results from an independent numerical method. The results show that the height distribution of the topography has a significant influence on the contact stiffness but that the curvature of the roughness is of minor importance. The contact model that was developed for determining the apparent contact area and the distribution of the mean contact pressure could thus be based on a limited set of height parameters that describe the surface topography. By operating on the calculated apparent pressure distribution with a transformation function that is based on both height and curvature parameters, the real contact area can be estimated when the apparent contact state is known. The model presented is also valid for cases with local plastic flow in the bulk material.
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| 39. |
- Shaikhibrahim, Zaki, 1979-
(author)
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Dynamics of protein folding and subunit interactions in assembly of the yeast mediator complex
- 2009
-
Doctoral thesis (other academic/artistic)abstract
- The Mediator complex was originally discovered in the yeast Saccharomyces cerevisiae and has since then been shown to be required for transcriptional regulation both in vitro and in vivo. The Mediator complex also stimulates basal, unregulated transcription and serves as a bridge by conveying signals from promoter-bound transcriptional regulatory proteins such as activators and repressors to the RNA Polymerase II general transcriptional machinery. The Mediator consists of 21 subunits and can be divided into three distinct modules head, middle and tail.Despite the tremendous progress that has been achieved so far in characterizing the Mediator complex both functionally and structurally, many aspects of the complex are not yet well understood. The objective of this work is to achieve further understanding of the Mediator complex by studying the folding of different protein subunits, their interactions and how that affects assembly of the Mediator complex.In our first study we made a temperature-sensitive med21 mutant and used it to identify genes that can suppress the mutation when present in high copy number. Among the 10 genes that we identified, the strongest suppressors were Med7 and Med10, which encode Mediator subunits, and Ash1, which encodes a repressor of the HO gene. We also used 2-hybrid experiments and immunoprecipitation to study protein-protein interactions between Med21 and the Med4, Med7 and Med10 proteins which are all essential for viability and located within the middle domain of the Mediator complex. We found that the N-terminal 2-8 amino acids of Med21 are required for interactions with Med7 and Med10. These results led us to propose a model in which the N-terminal part of Med21 functions as a molecular switchboard where competing signals from various activators, repressors and mediator subunits are integrated prior to reaching the general transcription machinery.In our second study, we extended our studies of protein-protein interactions to another part of the mediator complex by studying the folding and the assembly processes of the mediator head domain subunits Med8, Med18 and Med20. Using purified proteins and a combination of several different methods such as immunoprecipitation, far-UV circular dichroism and fluorescence, we demonstrated that the Med8, Med18 and Med20 subunits are interdependent on each other for proper folding and complex formation.
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| 40. |
- Shaikhibrahim, Zaki, 1979-, et al.
(author)
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Med8, Med18, and Med20 subunits of the Mediator head domain are interdependent upon each other for folding and complex formation
- 2009
-
In: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 106:49, s. 20728-20733
-
Journal article (peer-reviewed)abstract
- We have studied folding and complex formation of the yeast mediator head-module protein subunits Med8, Med18, and Med20. Using a combination of immunoprecipitation, far-UV circular dichroism, and fluorescence measurements on recombinantly expressed and denatured proteins that were allowed to renature separately or in different combinations, we found that Med8, Med18, and Med20 can fold in different ways to form both soluble monomeric proteins and different distinct subcomplexes. However, the concurrent presence of all three protein subunits during the renaturation process is required for proper folding and trimer complex formation.
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| 41. |
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| 42. |
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| 43. |
- Spiegelberg, Christer, et al.
(author)
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Simulation of transient friction of a cylinder between two planes
- 2003
-
In: Wear. - 0043-1648 .- 1873-2577. ; 254:11, s. 1170-1179
-
Journal article (peer-reviewed)abstract
- The need for good friction models for transient motions has increased as a consequence of the increased use of mechatronics and control engineering principles in precision mechanics. The machine elements in such equipment often involve rolling and sliding contacts. Most studies of friction in rolling and sliding contacts running under dry or boundary lubricated conditions have examined steady-state conditions. This paper describes simulations of the motion of a cylinder between two planes, first with a step change in velocity and then with an oscillating motion of the upper plane. The motion of the cylinder is determined by the friction in the contacts and the inertia. The friction in the rolling and sliding contacts is simulated with a brush model. The surfaces are assumed to be ideally smooth. For the step change in velocity, there is initially a period of complete sliding in the upper contact. During the sliding period, the friction force is the maximum possible, but it decreases as the complete sliding ends. The simulations show heavily damped oscillations, with frequencies corresponding to the natural translatory and torsional frequencies of the system. For the oscillating motions the sliding increases with the frequency of the motion, as expected.
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| 44. |
- Struglics, André, et al.
(author)
-
Western blot quantification of aggrecan fragments in human synovial fluid indicates differences in fragment patterns between joint diseases.
- 2009
-
In: Osteoarthritis and Cartilage. - : Elsevier BV. - 1063-4584. ; 17, s. 497-506
-
Journal article (peer-reviewed)abstract
- OBJECTIVE: To develop a Western blot method for quantification of multiple aggrecan fragments in human synovial fluids (SFs). METHOD: SF aggrecan fragments were prepared from knee healthy (reference), knee injury and arthritis subjects by CsCl gradient centrifugations collecting D1 fractions. Samples were analyzed by Western blot, using antibodies against the N-terminal epitope ARGS and the G3 domain, and fragments were quantified using a digital luminescence image analyzer. RESULTS: The method had a coefficients of variation of 10-30%, and a high correlation (r(S)=0.86) with a corresponding enzyme-linked immunosorbent assay (ELISA). The SFs from reference, knee injured and arthritic subjects contained two major ARGS fragments, ARGS-SELE and ARGS-CS1, and three major G3 fragments (GRGT-G3, GLGS-G3 and AGEG-G3). Compared to the reference, the acute arthritis and acute joint injury groups had a 30-fold elevated concentration of ARGS fragments, and both groups had a higher proportion of the aggrecan in joint fluid as ARGS fragments compared to the other groups. The reference and chronic injury groups had an excess of ARGS-CS1 fragments over ARGS-SELE fragments, while subjects with acute arthritis or osteoarthritis had a more even distribution between these fragments. CONCLUSIONS: We have developed a novel Western blot quantification method for quantification of SF aggrecan fragments which can differentiate fragments of different sizes sharing the same epitope. The anti-ARGS and anti-G3 quantitative Western blots provided information important for a better understanding of the proteolytic pathways in aggrecan breakdown, information that discriminates between different joint diseases, and may aid in identification of new biomarkers.
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| 45. |
- Sultan, Lena, et al.
(author)
-
Proterozoic and Archaean ages of detrital zircon from the Palaeoproterozoic Västervik Basin, SE Sweden: Implications for provenance and timing of deposition
- 2005
-
In: Gff. - 1103-5897. ; 127:Part 1, s. 17-24
-
Journal article (peer-reviewed)abstract
- Ages of detrital zircons, derived from Palaeoproterozoic metasedimentary rocks from Finland and Sweden are poorly represented in the presently exposed crust in the Baltic Shield. This study reports U-Pb ages of detrital zircons from the Svecofennian Vastervik Basin. 41 spots from 31 zircon crystals were dated using U-Pb geochronology at the NORDSIM ion microprobe in Stockholm. Most analyses are concordant and the zircon grains commonly display well-developed magmatic oscillatory zoning. The ages documented are: &SIM; 3.64 Ga, 3.03-2.95 Ga, 2.72-2.69 Ga, 2.12-1.87 Ga and 1.84 Ga. 75% of the grains are Palaeoproterozoic and 25% are Archaean. Ages gained from Proterozoic metasediments in Sweden, Finland, Svalbard, Greenland and Great Britain also report a large proportion of &SIM; 2.1-1.9 Ga and a smaller proportion of Archaean zircons with ages around 2.7 and 3 Ga. These age groups probably represent major crust forming events. The here presented results provide an estimate of the time of deposition in the Vastervik Basin for the time interval of 1882-1850 Ma, constrained by two concordant zircon analyses of 1872 &PLUSMN; 24 and 1870 &PLUSMN; 12 Ma, and the newly presented 1859 &PLUSMN; 9 Ma age for the Loftahammar granite that intrudes the metasedimentary succession in the north. The young detrital zircon age of 1837 &PLUSMN; 22 Ma might suggest that parts of the basin may be younger. As input of detrital grains may occur from several sources simultaneously (e.g. by rivers and by tidal currents from a marine source), the detrital grains were sampled from different depositional environments. Main fluvial sediment transport in the Vastervik Basin was from present north whereas the tidal sediment transport was from the present south. The age groups documented in the Vastervik Basin are poorly represented in the presently exposed crust in the Baltic Shield, but are represented in Sarmatia.
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| 46. |
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| 47. |
- Söderberg, Anders, et al.
(author)
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Validation of a simplified numerical contact model
- 2008
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In: Tribology International. - : Elsevier BV. - 0301-679X .- 1879-2464. ; 41:9-10, s. 926-933
-
Journal article (peer-reviewed)abstract
- Surface roughness tends to have a significant effect on how loads are transmitted at the contact interface between solid bodies. Most numerical contact models for analyzing rough surface contacts are computational demanding and more computationally efficient contact models are required. Depending on the purpose of the simulation, simplified and less accurate models can be preferable to more accurate, but also more complex, models. This paper discusses a simplified contact model called the elastic foundation model and its applicability to rough surfaces. The advantage of the model is that it is fast to evaluate, but its disadvantage is that it only gives an approximate solution to the contact problem. It is studied how surface roughness influences the errors in the elastic foundation solution in terms of predicted pressure distribution, real contact area, and normal and tangential contact stiffness. The results can be used to estimate the extent of error in the elastic foundation model, depending on the degree of surface roughness. The conclusion is that the elastic foundation model is not accurate enough to give a correct prediction of the actual contact stresses and contact areas, but it might be good enough for simulations where contact stiffness are of interest.
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| 48. |
- Victorsson, Jonas, 1968-
(author)
-
Community Assembly and Spatial Ecology of Saproxylic Coleoptera
- 2009
-
Doctoral thesis (other academic/artistic)abstract
- Community assembly is the process deciding inclusion/exclusion of species in a developing community. Spatial ecology integrates spatial habitat factors with local biotic interactions within a given patch. I used standardized tree bolts (0.35-1 m long) to sample saproxylic (wood living) beetles in natural and managed forest types. Density-dependent effects were more intense in old-growth coniferous forest than in burnt forest. The final-state assemblages in these two forest types diverged regarding species composition but converged regarding community structure. The communities also followed forest-type specific assembly trajectories. Order of arrival can influence the species assemblage, I found priority effects when comparing the reproductive success of two cerambycid species. A two-week head start had a positive effect on fitness in both species. Different fitness components were affected in the two species: offspring number in Acanthocinus aedilis and offspring quality in Rhagium inquisitor. In birch-living Coleoptera a large part of variance in species composition could be explained by habitat variables (22.9 %), such as forest type, or spatial variables (15.8 %), such as distance apart. The assemblages in deciduous sites responded to distance apart and showed positive spatial autocorrelation up to a distance of 80 km. For assemblages in deciduous sites a metacommunity perspective is warranted – on a surprisingly large scale. I compared two regions, one more fragmented and one less fragmented, (with 2.2 % more deciduous forest in the landscape). The effects of habitat fragmentation was primarily found in mature coniferous forest. Host-tree patches in this matrix forest were perceived as matrix by the Coleoptera in the more fragmented region but as habitat in the less fragmented region. Some of my study sites consisted of protected old-growth forest. These were embedded in a landscape dominated by forestry. These protected areas were invaded by generalist species, thriving in managed forests.
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| 49. |
- Walfridsson, Julian
(author)
-
The CHD chromatin remodeling factors in schizosaccharomyces pombe
- 2007
-
Doctoral thesis (other academic/artistic)abstract
- Regulation of chromatin structure is essential in a wide variety of processes including transcriptional regulation, recombination, replication, chromosome segregation, development and differentiation. The enzymes that are central in regulating chromatin structure can be classified into two major groups. The first group of proteins consists of the histone modifying enzymes that catalyse the addition or removal of posttranslational modifications of histones. The second group of proteins is the highly conserved ATP-dependent remodeling factors that modify the nucleosome structure. Evidence is emerging that these two groups of proteins are intimately linked in chromatin function. This thesis describes the roles of the S. pombe Hrp1 and Hrp3 CHD remodeling factors in chromatin regulation, which have been shown to be important in centromere function and transcriptional regulation. The Hrp remodeling factors are functionally linked to the histone chaperone Nap1 as well as acetylation and methylation activities. We have demonstrated that Hrp1 has both independent and overlapping roles with Hrp3 in regulating centromere assembly and function. Both hrp1 and hrp3 deficient cells are disrupted in centromere silencing and display various chromosome segregation defects indicative of functions at both the outer repeats and the central core of the centromere. These phenotypes are likely to originate from the requirement of Hrp1 in keeping the centromeres hypoacetylated and for maintaining the histone H3 variant CENP-A at the central core of the centromere. Genetic interactions combined with chromatin immunoprecipitation and fluorescent in situ hybridisation indicate that Hrp1 stimulates CENP-A assembly during DNA replication. In addition to their centromere functions, the Hrp remodeling factors contribute to transcriptional regulation by promoting histone removal. Biochemical purifications identified a physical interaction between Hrp1 and Hrp3 and with the histone chaperone Nap1. Consistent with the physical interaction data, genome wide analysis showed that the CHD remodeling factors together with Nap1 have a common function in removing histones particularly at promoter regions. Interestingly, we found that histone disassembly in coding regions by both Hrp1 and Hrp3 promote transcriptional activation. Cell synchronisation studies revealed that the Hrp1 dependent histone disassembly occurs in a DNA replication independent manner. A functional interaction between acetylation and remodeling activity was established based on the high degree of overlap between the Hrp ATPases, regions affected by Nap1 histone density, and corresponding histone deacetylase and histone acetylase targets. Finally, we discovered that regions with upregulated genes and altered levels of histone modifications in the HDAC clr6-1 mutant were significantly similar to equivalent lists for the histone demethyl transferase swm1 mutant. In addition, the same regions with upregulated genes and effects on histone modification levels in the swm1 and clr6 mutant overlapped with Hrp1 and Hrp3 binding targets. Thus, it is likely that Swm1 act in concert with Clr6 and Hrp1 to mediate transcriptional silencing. Thus, HDACs, HATs, and HMTs are intimately linked in vivo to CHD nucleosome remodeling factors as well as histone chaperones in centromere assembly and transcriptional regulation.
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| 50. |
- Xiao, Li, 1977, et al.
(author)
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The influence of surface roughness and the contact pressure distribution on friction in rolling/sliding contacts
- 2007
-
In: Tribology International. - Amsterdam : Elsevier. - 0301-679X .- 1879-2464. ; 40:4, s. 694-698
-
Journal article (peer-reviewed)abstract
- A numerical contact model is used to study the influence of surface roughness and the pressure distribution on the frictional behaviour in rolling/sliding contacts. Double-crowned roller surfaces are measured and used as input for the contact analysis. The contact pressure distribution is calculated for dry static contacts and the results are compared with friction measurements in a lubricated rolling/sliding contact made with a rough friction test rig. The mean pressure is suggested as a parameter that can be used to predict the influence of surface roughness on the friction coefficient in such contacts. The results show two important properties of the friction coefficient for the friction regime studied in this paper: (1) there is a linear decrease in friction coefficient as a function of the slide-to-roll ratio, and (2) the friction coefficient increases linearly with increasing mean contact pressure up to a maximum limit above which the friction coefficient is constant. The absolute deviation of experimental results from the derived theory is for most cases within 0.005.
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