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Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source
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- Reddy, Hemanth K. N. (author)
- Uppsala universitet,Molekylär biofysik
- Yoon, Chun Hong (author)
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- Aquila, Andrew (author)
- Uppsala universitet,Molekylär biofysik
- show more...
- Awel, Salah (author)
- Ayyer, Kartik (author)
- Barty, Anton (author)
- Berntsen, Peter (author)
- Bobkov, Sergey (author)
- Bucher, Maximilian (author)
- Carini, Gabriella A. (author)
- Carron, Sebastian (author)
- Chapman, Henry (author)
- DeMirci, Hasan (author)
- Fromme, Petra (author)
- Hart, Philip (author)
- Hogue, Brenda G. (author)
- Hosseinizadeh, Ahmad (author)
- Kim, Yoonhee (author)
- Kirian, Richard A. (author)
- Kurta, Ruslan P. (author)
- Loh, N. Duane (author)
- Mancuso, Adrian P. (author)
- Nam, Daewoong (author)
- Nettelblad, Carl (author)
- Ourmazd, Abbas (author)
- Rose, Max (author)
- Schwander, Peter (author)
- Song, Changyong (author)
- Spence, John C. H. (author)
- Vartanyants, Ivan A. (author)
- Williams, Garth J. (author)
- Xavier, P. Lourdu (author)
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- (creator_code:org_t)
- 2017-06-27
- 2017
- English.
- In: Scientia Danica. Series H. Humanistica 4. - : Nature Publishing Group. - 1904-5506 .- 2052-4463. ; 4
- Journal article (peer-reviewed)
Abstract
Subject headings
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- Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65-70 nm, which is considerably smaller than the previously reported similar to 600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.
Subject headings
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
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- ref (subject category)
- art (subject category)
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- Berntsen, Peter
- Bielecki, Johan
- Bobkov, Sergey
- Bucher, Maximili ...
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- Chapman, Henry
- Daurer, Benedikt
- DeMirci, Hasan
- Ekeberg, Tomas
- Fromme, Petra
- Hajdu, Janos
- Hantke, Max F.
- Hart, Philip
- Hogue, Brenda G.
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- Kim, Yoonhee
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- Munke, Anna
- Nam, Daewoong
- Nettelblad, Carl
- Ourmazd, Abbas
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- Song, Changyong
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