| 1. |
- Tegler, Lotta T., et al.
(författare)
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Powerful protein binders from designed polypeptides and small organic molecules : a general concept for protein recognition
- 2011
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Ingår i: Angewandte Chemie International Edition. - : Wiley. - 1433-7851 .- 1521-3773. ; 50:8, s. 1823-1827
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Tidskriftsartikel (refereegranskat)abstract
- High-affinity binders for the C-reactive protein (CRP), with dissociation constants in the pM to nM range and selectivities in human serum comparable to those of antibodies, were obtained by conjugation of 16 designed polypeptides to phosphocholine, a small molecule that binds CRP with a KDvalue of 5I . The polypeptides were not designed specifically to recognize CRP and bind by an adapted fit mechanism.
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| 2. |
- Dahlin, Andreas P., et al.
(författare)
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Methodological aspects on microdialysis protein sampling and quantification in biological fluids : an in vitro study on human ventricular CSF.
- 2010
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Ingår i: Analytical Chemistry. - : American Chemical Society (ACS). - 0003-2700 .- 1520-6882. ; 82:11, s. 4376-4385
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Tidskriftsartikel (refereegranskat)abstract
- There is growing interest in sampling of protein biomarkers from the interstitial compartment of the brain and other organs using high molecular cutoff membrane microdialysis (MD) catheters. However, recent data suggest that protein sampling across such MD membranes is a highly complex process that needs to be further studied. Here, we report three major improvements for microdialysis sampling of proteins in complex biological matrixes. The improvements in this in vitro study using human ventricular cerebrospinal fluid as the sample matrix include increased fluid recovery control, decreased protein adsorption on the microdialysis membrane and materials, and novel quantitative mass spectrometry analysis. Dextrans in different concentrations and sizes were added to the perfusion fluid. It was found that dextrans with molecular mass 250 and 500 kDa provided a fluid recovery close to 100%. An improved fluid recovery precision could be obtained by self-assembly triblock polymer surface modification of the MD catheters. The modified catheters also delivered a significantly increased extraction efficiency for some of the investigated proteins. The final improvement was to analyze the dialysates with isobaric tagged (iTRAQ) proteomics, followed by tandem mass spectrometric analysis. By using this technique, 48 proteins could be quantified and analyzed with respect to their extraction efficiencies. The novel aspects of microdialysis protein sampling, detection, and quantification in biological fluids presented in this study should be considered as a first step toward better understanding and handling of the challenges associated with microdialysis sampling of proteins. The next step is to optimize the developed methodology in vivo.
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| 3. |
- Nilsson, Lars, et al.
(författare)
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Starch and other polysaccharides
- 2012. - 1
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Ingår i: Field-flow fractionation in biopolymer analysis. - Vienna : Springer Vienna. - 9783709101537 - 9783709101544 ; , s. 165-185
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Bokkapitel (refereegranskat)abstract
- Polysaccharides constitute one of the major groups of biological macromolecules and they include some of the most abundant macromolecules in nature. In this chapter instrumental considerations when analyzing polysaccharides with flow field-flow fractionation (flow FFF) are briefly discussed. Furthermore, an overview of characterized properties is given with special attention to multi angle light scattering. Included is an extensive review of literature on applications regarding flow FFF and polysaccharides.
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| 4. |
- Wahlund, Karl-Gustav, et al.
(författare)
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Flow FFF–basics and key applications
- 2012. - 1
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Ingår i: Field-flow fractionation in biopolymer analysis. - Vienna : Springer Vienna. - 9783709101537 - 9783709101544 ; , s. 1-21
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Bokkapitel (refereegranskat)abstract
- The 1990s and 2000s have seen a rapidly growing use of flow field-flow fractionation (flow FFF, FlFFF). As of today hundreds of publications in many different application areas are presented each year in which flow FFF has been used or is referred to. In this chapter a brief historical overview of flow FFF is given. Channel designs and basic principles are discussed as well as approaches to development of rapid high resolution separations. Finally, an overview of key applications is included with pioneering and ground-breaking papers from literature.
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| 5. |
- Williams, S. Kim R., et al.
(författare)
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Field-flow fractionation
- 2014
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Ingår i: Analytical and Bioanalytical Chemistry. - : Springer Science and Business Media LLC. - 1618-2642 .- 1618-2650. ; 406:6, s. 1577-1578
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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