| 1. |
- Ludvigsson, Johnny, et al.
(författare)
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GAD65 antigen therapy in recently diagnosed type 1 diabetes mellitus
- 2012
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Ingår i: New England Journal of Medicine. - : Massachusetts Medical Society. - 0028-4793 .- 1533-4406. ; 366:5, s. 433-442
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Tidskriftsartikel (refereegranskat)abstract
- BACKGROUND: The 65-kD isoform of glutamic acid decarboxylase (GAD65) is a major autoantigen in type 1 diabetes. We hypothesized that alum-formulated GAD65 (GAD-alum) can preserve beta-cell function in patients with recent-onset type 1 diabetes.METHODS: We studied 334 patients, 10 to 20 years of age, with type 1 diabetes, fasting C-peptide levels of more than 0.3 ng per milliliter (0.1 nmol per liter), and detectable serum GAD65 autoantibodies. Within 3 months after diagnosis, patients were randomly assigned to receive one of three study treatments: four doses of GAD-alum, two doses of GAD-alum followed by two doses of placebo, or four doses of placebo. The primary outcome was the change in the stimulated serum C-peptide level (after a mixed-meal tolerance test) between the baseline visit and the 15-month visit. Secondary outcomes included the glycated hemoglobin level, mean daily insulin dose, rate of hypoglycemia, and fasting and maximum stimulated C-peptide levels.RESULTS: The stimulated C-peptide level declined to a similar degree in all study groups, and the primary outcome at 15 months did not differ significantly between the combined active-drug groups and the placebo group (P=0.10). The use of GAD-alum as compared with placebo did not affect the insulin dose, glycated hemoglobin level, or hypoglycemia rate. Adverse events were infrequent and mild in the three groups, with no significant differences.CONCLUSIONS: Treatment with GAD-alum did not significantly reduce the loss of stimulated C peptide or improve clinical outcomes over a 15-month period.
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| 2. |
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| 3. |
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| 4. |
- Abrahamsson, Christina, et al.
(författare)
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DeltaT50 - a new method to assess temporal ventricular repolarization variability
- 2011
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Ingår i: Journal of Electrocardiology. - : Elsevier BV. - 1532-8430 .- 0022-0736. ; 44:4
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Tidskriftsartikel (refereegranskat)abstract
- Background Increased beat-to-beat variability in cardiac repolarization time is a tentative risk marker of drug-induced torsades de pointes. We developed a new, automatic method based on the temporal variability of the T-wave down slope to assess this variability. Method and Results Leads V1 to V6 of resting electrocardiograms were recorded in 42 healthy subjects (18-68 years, 22 men). The temporal variability at 50% of the T-wave down slope, deltaT50 (1.5 ± 0.41 milliseconds; range, 0.86-2.66 milliseconds), was measured with an accuracy of 1 millisecond on at least 9 pairs of electrocardiogram complexes with a signal-to-noise ratio more than 10 and changes in the R-R interval less than 150 milliseconds. The correlation between repeated measurements of deltaT50 was high. DeltaT50 was measured without corrections for age, sex, heart rate, T-wave amplitude, signal-to-noise ratio, R-R variability, and QTcF because none of these factors explained more than 4% of the within-subject deltaT50 variability. Conclusion The beat-to-beat repolarization variability was measured with high fidelity with the deltaT50 method and was a robust measure in healthy volunteers.
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| 5. |
- Andersson, Leif, et al.
(författare)
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Inventering av violgubbe (Gomphus clavatus) och rödlistade fjälltaggsvampar (Sarcodon spp.) i Västmanlands län 2008.
- 2010
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Denna inventering av violgubbe (Gomphus clavatus) och rödlistade fjälltaggsvampar (Sarcodon spp.) har utförts på uppdrag av Länsstyrelsen i Västmanlands län. Syftet har varit att öka kunskapen om arternas förekomst i länet samt behovet av skydd och andra åtgärder på de olika lokalerna. Inventeringen har genomförts inom ramen för åtgärdsprogrammen (ÅGP) för bevarande av violgubbe och rödlistade fjälltaggsvampar. Inventeringen har genomförts under september 2008 av Leif Andersson, Kurt- Anders Johansson, Rolf-Göran Carlsson, Stig Jacobsson och Tomas Fasth. Alla kända lokaler för violgubbe och rödlistade fjälltaggsvampar besöktes. Även lokaler för arter med likartade miljökrav har besökts och områden kända för kalkrik berggrund. Under inventeringen har mycel för de eftersökta arterna noterats och koordinater för dessa tagits. Även andra svampar har noterats om än i olika omfattning. Totalt har ett hundratal lokaler besökts varav svampar har noterats på 80 lokaler. Säsongen var mycket god för fjälltaggsvampar, möjligen med undantag för brödtaggsvamp (Sarcodon versipellis). För violgubbe var säsongen något sämre. Totalt noterades violgubbe och/eller rödlistade fjälltaggsvampar på 17 lokaler. Av de 19 kända lokalerna för violgubbe noterades den på sex och en ny lokal upptäcktes. Tidigare var 20 lokaler kända för de olika rödlistade fjälltaggsvamparna varav återfynd gjordes på sex av dessa. Dessutom gjordes 14 nya fynd av rödlistade fjälltaggvampar. Nya för Västmanlands län är blåfotad taggsvamp (Sarcodon glaucopus), sammetstaggsvamp (Sarcodon martioflavus) och den ännu obeskrivna Sarcodon ”modestum”. Den goda svamptillgången 2008 gjorde att särskilt många rödlistade spindelskivlingar noterades. Flera arter är nya för Västmanlands län. Även andra nyfynd av rödlistade svampar för Västmanlands län gjordes t. ex. narrvaxskivlingen (Hygrophorus subviscifer) som noterades på två platser. Totalt gjordes 146 fynd av 42 rödlistade svampar inklusive violgubbe och rödlistade fjälltaggsvampar. Ett antal lokaler och områden har kristalliserats ut som särskilt intressanta för de eftersökta svamparna. I Sala kommun finns många värdefulla områden, särskilt nära tätorten, ofta i närheten av där gruvdrift bedrivits. Särskilt Snarpsätraskogen norr om staden samt områden söder och sydväst om staden hyste många hotade arter. Områdena söder om staden ingår i Salakalkens Natura 2000-område, men skogsnaturtyperna är i dagsläget inte anmälda som naturtyp. I Fagersta och Norberg finns också värdefulla stråk för violgubbe och rödlistade fjälltaggsvampar. Särskilt ska här framhållas Jättåsarna i Fagersta kommun. Flera områdena är inte utpekade som naturvärden eller nyckelbiotoper och saknar också formellt områdesskydd. Ett antal lokaler för de eftersökta svamparna finns i naturreservat och biotopskydd.
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| 6. |
- Bartha, Erzsebet, et al.
(författare)
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Cost-effectiveness Analysis of Goal-directed Hemodynamic Treatment of Elderly Hip Fracture Patients : Before Clinical Research Starts
- 2012
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Ingår i: Anesthesiology. - : Lippincott Williams & Wilkins. - 0003-3022 .- 1528-1175. ; 117:3, s. 519-530
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Tidskriftsartikel (refereegranskat)abstract
- Background: Health economic evaluations are increasingly used to make the decision to adopt new medical interventions. Before such decisions, various stakeholders have invested in clinical research. But health economic factors are seldom considered in research funding decisions. Cost-effectiveness analysis could be informative before the launch of clinical research projects, particularly when a targeted intervention is resource-intensive, total cost for the trial is very high, and expected gain of health benefits is uncertain. This study analyzed cost-effectiveness using a decision analytic model before initiating a large clinical research project on goal-directed hemodynamic treatment of elderly patients with hip fracture.Methods: A probabilistic decision analytic cost-effectiveness model was developed; the model contains a decision tree for the postoperative short-term outcome and a Markov structure for long-term outcome. Clinical effect estimates, costs, health-related quality-of-life measures, and long-term survival constituted model input that was extracted from clinical trials, national databases, and surveys. Model output consisted of estimated medical care costs related to quality-adjusted life-years.Results: In the base care analysis, goal-directed hemodynamic treatment reduced average medical care costs by €1,882 and gained 0.344 qualilty-adjusted life-years. In 96.5% of the simulations, goal-directed hemodynamic treatment is less costly and provides more quality-adjusted life-years. The results are sensitive to clinical effect size variations, although goal-directed hemodynamic treatment seems to be cost-effective even with moderate clinical effect.Conclusion: This study demonstrates that cost-effectiveness analysis is feasible, meaningful, and recommendable before launch of costly clinical research projects.
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| 7. |
- Bartha, Erzsebet, et al.
(författare)
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Cost-effectiveness Analysis of Goal-directed Hemodynamic Treatment of Elderly Hip Fracture Patients
- 2012
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Ingår i: Anesthesiology. - : ASA Publishers. - 0003-3022 .- 1528-1175. ; 117:3, s. 519-530
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Tidskriftsartikel (refereegranskat)abstract
- BackgroundHealth economic evaluations are increasingly used to make the decision to adopt new medical interventions. Before such decisions, various stakeholders have invested in clinical research. But health economic factors are seldom considered in research funding decisions. Cost-effectiveness analyses could be informative before the launch of clinical research projects, particularly when a targeted intervention is resource-intensive, total cost for the trial is very high, and expected gain of health benefits is uncertain. This study analyzed cost-effectiveness using a decision analytic model before initiating a large clinical research project on goal-directed hemodynamic treatment of elderly patients with hip fracture.MethodsA probabilistic decision analytic cost-effectiveness model was developed; the model contains a decision tree for the postoperative short-term outcome and a Markov structure for long-term outcome. Clinical effect estimates, costs, health-related quality-of-life measures, and long-term survival constituted model input that was extracted from clinical trials, national databases, and surveys. Model output consisted of estimated medical care costs related to quality-adjusted life-years.ResultsIn the base case analysis, goal-directed hemodynamic treatment reduced average medical care costs by €1,882 and gained 0.344 quality-adjusted life-years. In 96.5% of the simulations, goal-directed hemodynamic treatment is less costly and provides more quality-adjusted life-years. The results are sensitive to clinical effect size variations, although goal-directed hemodynamic treatment seems to be cost-effective even with moderate clinical effect.ConclusionThis study demonstrates that cost-effectiveness analysis is feasible, meaningful, and recommendable before launch of costly clinical research projects.
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| 8. |
- Beausang, Angela, et al.
(författare)
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"Möjligheten att rädda några av dessa kvinnors liv har inte vägts in"
- 2014
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Ingår i: Dagens Medicin. - : Dagens Medicin.
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Tidskriftsartikel (populärvet., debatt m.m.)abstract
- Namnet på Socialstyrelsens vägledning lyder: Hur upptäcka våldsutsatthet? Ja, det kan man verkligen fråga sig efter att ha läst detta föga vägledande dokument, skriver ett stort antal kritiska debattörer.
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| 9. |
- Bosaeus, Ingvar, 1950, et al.
(författare)
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Vårdprogram vid tarmsvikt
- 2010
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Ingår i: Svensk Förening för Gastroenterologi.
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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| 10. |
- Brämming, Mats
(författare)
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Avoiding slopping in top-blown BOS vessels
- 2010
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Slag formation plays a decisive role in all steelmaking processes. In top-blowing Basic Oxygen Steelmaking (BOS), i.e. in the LD process, an emulsion consisting of liquid slag, dispersed metal droplets and solid particles will, together with process gases, form an expanding foam. Extensive research has defined the parameters that govern the foaming characteristics of BOS slag emulsions. It is a well known fact that certain process conditions will lead to an excessive foam growth, forcing the foam out through the vessel opening (mouth). This process event is commonly known as slopping. Slopping results in loss of valuable metal, equipment damage, lost production time and pollution. A literature survey covering the slopping phenomena has been carried out, as well as a deeper investigation into the causes behind slopping on the BOS vessels, type LD/LBE, at SSAB EMEA Metallurgy Luleå, equipped with an automatic system for slopping registration using image analysis. Good slag formation and foam-growth control in order to avoid slopping is primarily accomplished by taking preventive "static" measures. Improved slopping control has been achieved by developing a new oxygen lance control scheme, featuring adjustment of the distance between the lance tip and the metal bath according to scrap quality and ore additions. If "static" measures cannot be effectuated, in-blow control measures are needed. For such "dynamic" measures to be effective, it is necessary to have a system for slopping prediction. In the early-1980s a system for foam level and slopping control, based on BOS vessel vibration, was temporarily installed and tested on one of the vessels in Luleå. Based on the experiences from these tests it was decided to re-investigate the vessel vibration measurement technique. Trials on industrial scale BOS vessels of type LD/LBE have been carried out. FFT spectrum analysis has been applied in order to find the frequency band with best correlation to an estimated foam height. The results show that there is a correlation between vessel vibration and foam height which can be used for dynamic foam level and slopping control. The vessel vibration results have been tested against perhaps the most common implemented technique for dynamic foam height estimation and slopping control, the audiometric system. Parallel vibration and audio measurements have been carried out on a 130-tonne as well as on 300-tonne BOS vessels. The results show that during stable process conditions there is good agreement between the two methods with regard to foam height estimation and that combining the two methods will provide a powerful slopping prediction and control system.
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| 11. |
- Bucardo, Filemon, et al.
(författare)
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Susceptibility of Children to Sapovirus Infections, Nicaragua, 2005–2006
- 2012
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Ingår i: Emerging Infectious Diseases. - Atlanta, GA, USA : U.S. Department of Health and Human Services * Centers for Disease Control and Prevention. - 1080-6040 .- 1080-6059. ; 18:11, s. 1875-1878
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Tidskriftsartikel (refereegranskat)abstract
- We describe the genetic diversity of sapovirus (SaV) in children in Nicaragua and investigate the role of host genetic factors and susceptibility to SaV infections. Our results indicate that neither ABO blood group, Lewis phenotype, nor secretor status affects susceptibility to SaV infection in Nicaragua.
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| 12. |
- Carlsson, Andreas, et al.
(författare)
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Låg kunskap om trötthet ger tröttare idrottare
- 2013
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Ingår i: Svensk Idrottsforskning. - 1103-4629. ; 22:1, s. 28-32
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- En intervjustudie med idrottare och deras tränare visar på likheter, men också en del intressanta skillnader i sättet att se på trötthetsbegreppet. Artikeln diskuterar vad det kan bero på och ger förslag på hur tränare kan bli bättre på att läsa av sina utövares trötthet.
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| 13. |
- Carlsson, Bengt-Göran
(författare)
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Klimatövervakning på Åreskutan : En jämförande studie av vegetationens sammansättning vid två inventeringstillfällen med fem års intervall (2006 och 2011). FjällNILS-projektet (Vegetation)
- 2011
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Rapport (populärvet., debatt m.m.)abstract
- Åreskutans vegetation ovanför subalpin zon har inom samma provytor studerats vid två tillfällen med5 års mellanrum (2006 och 2011) med hjälp av en inventeringsmetodik som är hämtad och utveckladfrån NILS‐projektet. En jämförelse mellan åren visar att fältskiktets täckning totalt sett minskadenågot framförallt beroende på ett omfattande sorkbete 2011. Gräsväxterna minskade i fem av de sexundersökta transekterna, medan en svag ökning skedde i en transekt (C). Sorkbetets betydelse förnedgången 2011 visas av en kraftig ökning av förna detta år. På provytor med karg vegetation, somvisar få eller inga tecken på sorkbete, har istället gräs‐ och ljungväxter ökat mellan åren.Genomgående för alla transekter är en tydlig ökning av dvärgviden. Denna ökning blir speciellt tydligi provytor ovanför 1100 meter över havet. Fältskiktets täckning minskade generellt mellan åren i ytorunder 1100 meter, men ökade ovanför denna nivå. I bottenskiktet minskade andelen övriga mossor,speciellt på låg höjd, troligen som en följd av att mossorna överlagrats av förna från sorkbetadeväxter. På karga ytor med svagt utvecklat fältskikt, och därmed lite påverkat av sorkbete, harmossorna istället ökat sin täckning mellan åren. Busklavarna minskade tydligt i alla transekter ochspeciellt i provytor under 1250 meter över havet. Antalet provytor med trädförekomst ökade medtvå ytor (4%) mellan åren. Gran tillkom i en yta 2011 jämfört med 2006, medan glasbjörk försvannfrån en yta under samma period. Totalt sett hittades fler kärlväxtarter 2011 än 2006 i alla transekter(lika antal i en transekt) och i alla höjdintervall. Den vanligaste arten 2006 var kråkbär, medanstyvstarr var vanligast 2011.Vid bearbetningen av data från de båda åren har det framgått hur svårt det är för inventeraren atthålla samma bedömningsnivå för täckningsgrad vid olika inventeringstillfällen separerade i tid. Dettamedför svårigheter att utvärdera eventuella skillnader mellan olika år. I rapportern föreslås atttäckningsgradsbedömningar utesluts för fält‐ och bottenskikt i de större, och därmed mersvårbedömda provytorna (10m‐ytorna), vid framtida inventeringar. Istället föreslås att antaletsmåytor utökas eftersom dessa är mer lättbedömda och där det också kommer att finnas ett brabildmaterial som gör det möjligt att verifiera täckningsgradsbedömningar bakåt i tiden, åtminstonegäller detta för fältskiktet.
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| 14. |
- Carlsson, Bengt-Göran
(författare)
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Övervakning av fjällvegetation på Hundshögen : Förändringar mellan åren 2007 och 2012
- 2013
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Rapport (populärvet., debatt m.m.)abstract
- Studiens syfte var att studera vegetationens utveckling i höjdgradienter ovanför subalpin zon på Hundshögen (1 372 meter över havet) i Jämtland, för att se eventuella klimatrelaterade effekter på floran. Från fjällets topp utplacerades 49 provytor fördelade i fem transekter i ett stjärnsystem med 250 meters mellanrum mellan provytorna. De permanent markerade provytorna inventerades vid två tillfällen med fem års mellanrum (2007 och 2012). Fältskiktets totala täckningsgrad skilde sig obetydligt mellan åren, men förändringar hade skett i några av dess komponenter.Graminidernas täckning visade en tydligt minskande trend mellan åren, till skillnad från ljungväxterna, framförallt kråkbär, och dvärgvide som ökade sin täckning. Förändringarna kan i första hand relateras till en mycket omfattande sorktopp under åren 2010–2011. I bottenskiktet ökade andelen förna/humus mellan åren, medan mossor och busklavar minskade något, framförallt beroende på en överlagring av förna. I buskskiktet har dvärgbjörk och enbuskar spritt sig till nya ytor under femårsperioden, i synnerhet för dvärgbjörk har spridningen skett i dess övre utbredningsområde.För träden (glasbjörk och tall) har det skett en markant nyetablering av plantor i höga lägen. Plantor av glasbjörk hittades 2012 på drygt 300 meter högre höjd än 2007, tall saknades helt i provytorna 2007. Den biologiska mångfalden har ökat, fler arter har tillkommit, än de som försvunnit under perioden.Flera av de befintliga arterna 2007 har spridit sig till nya provytor 2012. Några arter visar en negativ trend till exempel lopplummer och axfryle. Framförallt förändringar i busk- och trädskikt, samt förändringar i biologisk mångfald, bör kunna relateras till de pågående klimatförändringarna som leder till en förlängd växtsäsong.
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| 15. |
- Carlsson, Göran, 1951, et al.
(författare)
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Incidence of severe congenital neutropenia in Sweden and risk of evolution to myelodysplastic syndrome/leukaemia.
- 2012
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Ingår i: British journal of haematology. - : Wiley. - 1365-2141 .- 0007-1048. ; 158:3, s. 363-369
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Tidskriftsartikel (refereegranskat)abstract
- Severe congenital neutropenia (SCN) is characterized by low blood neutrophil counts, early bacterial infections, and risk of leukaemia development. As yet, no population-based incidence estimates of SCN have been reported. Children less than 16years of age with SCN were sought in Sweden during the 20-year period 1987-2006 by a questionnaire to all Swedish Departments of Paediatrics and by reviewing the Swedish Health and Welfare Statistical Databases. Thirty-two patients were diagnosed with congenital neutropenia during this period. All received treatment with recombinant granulocyte-colony stimulating factor (G-CSF). Twenty-one patients were diagnosed as SCN or probable SCN, corresponding to 1·0 per 100000 live births. Nine (43%) had ELANE mutations, four (19%) HAX1 mutations and eight (38%) were children with disease of unknown genetic aetiology. Four out of 21 patients (19%) developed myelodysplastic syndrome/leukaemia and three (14%) died, all with leukaemia. The cumulative incidence of myelodysplastic syndrome/leukaemia was 31%. The observed incidence of SCN in this population-based study was higher than previously estimated, possibly because genetic testing now can identify SCN cases previously thought to be idiopathic or benign neutropenia. The risk of developing myelodysplastic syndrome/leukaemia is considerable. ELANE mutations are the most commonly identified genetic defects.
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| 16. |
- Carlsson, Göran, 1951, et al.
(författare)
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Pretherapeutic uracil and dihydrouracil levels in saliva of colorectal cancer patients are associated with toxicity during adjuvant 5-fluorouracil-based chemotherapy.
- 2014
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Ingår i: Cancer chemotherapy and pharmacology. - : Springer Science and Business Media LLC. - 1432-0843 .- 0344-5704. ; 74:4, s. 757-63
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Tidskriftsartikel (refereegranskat)abstract
- 5-fluorouracil (5-FU) competes with uracil (Ura) as a substrate for dihydropyrimidine dehydrogenase (DPD). Low DPD activity impairs breakdown of Ura to dihydrouracil (UH2) and is associated with toxicity during 5-FU-based chemotherapy. Calculation of the 5-FU dose is based on body surface area, and new tools are needed to individualize treatment. The aim of study was to measure Ura and UH2 in saliva of patients with colorectal cancer and relate levels to treatment-induced toxicity.
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| 17. |
- Carlsson, Noomi
(författare)
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A Zero-vision for Children’s Tobacco Smoke Exposure : Tobacco prevention in Child Health Care
- 2012
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Adverse health effects in children caused by environmental tobacco smoke (ETS) are well known. Children are primarily exposed by their parents’ smoking in their homes. A comprehensive evidence base shows that parental smoking during pregnancy and ETS exposure in early childhood are associated with an increased risk for a range of adverse health problems. Child Health Care nurses, who meet nearly all families in Sweden with children aged 0-6 years, have thus an important role in tobacco preventive work in order to support parents in their ambitions to protect their children from ETS exposure.The overall aim of this thesis was to develop, test and evaluate a new model for tobacco preventive work in Child Health Care (CHC) with special focus on areas with a high prevalence of parental smoking. In a first step CHC nurses’ and parents’ views on tobacco preventive work were analysed in two studies based on questionnaires.The intervention was performed during the second step, based on the results from nurses’ and parents’ experience of the tobacco preventive work in CHC, and with methods from Quality Improvement. An “intervention bundle” was developed which included evidence based methods for prevention of ETS exposure, and four learning sessions for the nurses. The instrument “Smoking in Children’s Environment Test” (SiCET) included in the bundle was evaluated with focus group interviews with the CHC nurses who participated in the intervention. Two urine samples were analysed to measure cotinine levels in children which provide an estimate for ETS exposure. Parents’ answers from the SiCET questionnaire, measurements of cotinine, and data from the nurses’ log-books were used in the evaluation of the effects of the intervention. In areas with a high prevalence of parental smoking 22 nurses recruited 86 families of whom 72 took part for the entire one-year period of the intervention.The results showed that parents wanted to have information on the harmful effects tobacco smoke have on their children and how they can protect their children from ETS exposure. The nurses saw tobacco preventive work as important but they experienced difficulties to reach certain groups such as fathers, foreign-born parents, and those who are socio-economically disadvantaged. The SiCET instrument provided a basis for dialogue with parents. The main results from the intervention showed that ten parents (11%) quit smoking, thirty-two families (44%) decreased their cigarette consumption in the home, and fewer children were exposed to tobacco smoke. Consequently, more children showed levels of urinary cotinine less than 6 ng/ml (base-line n=43, follow up n=54; p=0.05). The total number of outdoor smokers did not change. Seven of the nurses (30%) had successful results in their areas with a decrease of smokers in families with a child of 8 months, from 20% in 2009 to 12% in 2011. The corresponding figures for the whole county as well as the country did not decrease during the same period.The sustainability of the intervention has to be followed and thus measures should be followed prospectively over time. The SiCET instrument was found useful and might be applicable in other arenas where children’s ETS exposure is discussed. The development of an instant cotinine test using dipsticks would make it possible to give parents immediate feedback on the effectiveness of taken protective actions. This could work as a pedagogic resource in the dialogue with parents.
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| 18. |
- Carlsson, Noomi, et al.
(författare)
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Child health nurses' roles and attitudes in reducing children's tobacco smoke exposure
- 2010
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Ingår i: Journal of Clinical Nursing. - : John Wiley & Sons. - 0962-1067 .- 1365-2702. ; 19:3-4, s. 507-516
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Tidskriftsartikel (refereegranskat)abstract
- AIM: To investigate and analyse the attitudes to tobacco prevention among child healthcare nurses, to study how tobacco preventive work is carried out at child healthcare centres today. To evaluate how the tobacco preventive work had changed in child health care since the Swedish National Board of Health and Welfare's national evaluation in 1997.BACKGROUND: Exposure to environmental tobacco smoke has adverse health effects. Interventions aiming at minimising environmental tobacco smoke have been developed and implemented at child healthcare centres in Sweden but the long-term effects of the interventions have not been studied.DESIGN: Survey.METHODS: In 2004, a postal questionnaire was sent to all nurses (n = 196) working at 92 child healthcare centres in two counties in south-eastern Sweden. The questionnaire was based on questions used by the National Board of Health and Welfare in their national evaluation in 1997 and individual semi-structured interviews performed for this study.RESULTS: Almost all the nurses considered it very important to ask parents about their smoking habits (median 9.5, range 5.1-10.0). Collaboration with antenatal care had decreased since 1997. Nearly all the nurses mentioned difficulties in reaching fathers (70%), groups such as immigrant families (87%) and socially vulnerable families (94%) with the tobacco preventive programme. No nurses reported having special strategies to reach these groups.CONCLUSIONS: Improvement of methods for tobacco prevention at child healthcare centres is called for, especially for vulnerable groups in society. However, the positive attitude among nurses found in this study forms a promising basis for successful interventions.RELEVANCE TO CLINICAL PRACTICE: This study shows that launching national programmes for tobacco prevention is not sufficient to achieve sustainable work. Nurses working in child healthcare centres have an overall positive attitude to tobacco prevention but need continuous education and training in communication skills especially to reach social vulnerable groups. Regular feedback from systematic follow-ups might increase motivation for this work.
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| 19. |
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| 20. |
- Carlsson, Noomi, et al.
(författare)
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Parents' attitudes to smoking and passive smoking and their experience of the tobacco preventive work in child health care
- 2011
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Ingår i: Journal of Child Health Care. - : Sage Publications. - 1367-4935 .- 1741-2889. ; 15:4, s. 272-286
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Tidskriftsartikel (refereegranskat)abstract
- The purpose of this study was to describe parents' attitudes to smoking and their experience of the tobacco preventive work in antenatal care and in Child Health Care (CHC) in Sweden. A population based survey in which 62 percent of 3000 randomly selected parents with 1- and 3-year-old children answered a questionnaire. Fifty-six percent stated that smoking was registered in the health record of the child yet no further discussion regarding passive smoking took place. The parents' educational level and smoking status was related to the attitudes and experiences of the tobacco preventive work. The results indicated that the dialogue with parents regarding children and environmental tobacco smoke (ETS) exposure has to be redesigned and intensified in order to meet the needs of parents with different backgrounds.
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| 21. |
- Carlsson, Åsa, 1978, et al.
(författare)
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Degraded water suppression in small volume ¹H MRS due to localised shimming.
- 2011
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Ingår i: Magma (New York, N.Y.). - : Springer Science and Business Media LLC. - 0968-5243. ; 24:2, s. 97-107
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Tidskriftsartikel (refereegranskat)abstract
- High quality, artifact free, ¹H MRS require a homogeneous magnetic field over the volume of interest (VOI) and an effective global water suppression (WS). However, the shim coils act globally and, hence, affect the resonance frequency over the entire object, making the frequency selective WS spatially selective. Unsuppressed water signal may cause spurious echo artifacts. Aim: Study and visualise the effect localised shimming has on WS.
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| 22. |
- de la Cruz Rodríguez, Jaime, 1983-
(författare)
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Measuring the solar atmosphere
- 2010
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- The new CRISP filter at the Swedish 1-m Solar Telescope provides opportunities for observing the solar atmosphere with unprecedented spatial resolution and cadence. In order to benefit from the high quality of observational data from this instrument, we have developed methods for calibrating and restoring polarized Stokes images, obtained at optical and near infrared wavelengths, taking into account field-of-view variations of the filter properties. In order to facilitate velocity measurements, a time series from a 3D hydrodynamical granulation simulation is used to compute quiet Sun spectral line profiles at different heliocentric angles. The synthetic line profiles, with their convective blueshifts, can be used as absolute references for line-of-sight velocities. Observations of the Ca II 8542 Å line are used to study magnetic fields in chromospheric fibrils. The line wings show the granulation pattern at mid-photospheric heights whereas the overlying chromosphere is seen in the core of the line. Using full Stokes data, we have attempted to observationally verify the alignment of chromospheric fibrils with the magnetic field. Our results suggest that in most cases fibrils are aligned along the magnetic field direction, but we also find examples where this is not the case. Detailed interpretation of Stokes data from spectral lines formed in the chromospheric data can be made using non-LTE inversion codes. For the first time, we use a realistic 3D MHD chromospheric simulation of the quiet Sun to assess how well NLTE inversions recover physical quantities from spectropolarimetric observations of Ca II 8542 Å. We demonstrate that inversions provide realistic estimates of depth-averaged quantities in the chromosphere, although high spectral resolution and high sensitivity are needed to measure quiet Sun chromospheric magnetic fields.
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| 23. |
- Derwinger, Kristoffer, 1969, et al.
(författare)
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A phase I/II study of neoadjuvant chemotherapy with Pemetrexed (Alimta) in rectal cancer.
- 2011
-
Ingår i: European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology. - : Elsevier BV. - 1532-2157. ; 37:7, s. 583-8
-
Tidskriftsartikel (refereegranskat)abstract
- The aim was to assess the feasibility of preoperative chemotherapy and possible tumour response using Pemetrexed (Alimta) in rectal cancer.
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| 24. |
- Derwinger, Kristoffer, 1969, et al.
(författare)
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Defining stage III disease in colorectal cancer--aspects on treatment and evaluation of survival.
- 2010
-
Ingår i: Journal of surgical oncology. - : Wiley. - 1096-9098 .- 0022-4790. ; 102:5, s. 424-7
-
Tidskriftsartikel (refereegranskat)abstract
- Stage III in colorectal cancer is defined by presence of node metastasis, whereas distant growth constitutes stage IV. The aim was to describe prognosis in high risk groups of stage III in relation to survival in stage IV, along with possible effect on research and treatment.
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| 25. |
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| 26. |
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| 27. |
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| 28. |
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| 29. |
- Gren Landell, Malin, 1968-, et al.
(författare)
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Posttraumatic stress symptoms and mental health services utilization in adolescents with social anxiety disorder and experiences of victimization
- 2013
-
Ingår i: European Child and Adolescent Psychiatry. - : Springer. - 1018-8827 .- 1435-165X. ; 22:3, s. 177-184
-
Tidskriftsartikel (refereegranskat)abstract
- Recent findings from studies on adults show similarities between social anxiety disorder (SAD) and posttraumatic stress in the form of recurrent memories and intrusive and distressing images of earlier aversive events. Further, treatment models for SAD in adults have been successfully developed by using transdiagnostic knowledge on posttraumatic stress symptoms (PTSS). Studies on adolescents are though missing. The present study aimed at exploring the association between PTSS and SAD in Swedish adolescents. A second aim was to study mental health services utilization in relation to these conditions. A total of 5,960 high-school students participated and reported on SAD, life time victimization, PTSS and mental health service utilization. Socially anxious adolescents reported significantly higher levels of PTSS than adolescents not reporting SAD and this difference was seen in victimized as well as non-victimized subjects. Contact with a school counselor was the most common mental health service utilization in subjects with SAD and those with elevated PTSS. In the prediction of contact with a CAP-clinic, significant odds ratios were found for a condition of SAD and elevated PTSS (OR = 4.88, 95 % CI = 3.53–6.73) but not for SAD only. Screening of PTSS in adolescents with SAD is recommended. The service of school counselors is important in detecting and helping young people with SAD and elevated PTSS. Clinical studies on SAD and PTSS in adolescents could aid in modifying treatment models for SAD.
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| 30. |
- Götberg, Matthias, et al.
(författare)
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A Pilot Study of Rapid Cooling by Cold Saline and Endovascular Cooling Before Reperfusion in Patients With ST-Elevation Myocardial Infarction.
- 2010
-
Ingår i: Circulation. Cardiovascular Interventions. - 1941-7632. ; 3, s. 400-407
-
Tidskriftsartikel (refereegranskat)abstract
- Background-Experimental studies have shown that induction of hypothermia before reperfusion of acute coronary occlusion reduces infarct size. Previous clinical studies, however, have not been able to show this effect, which is believed to be mainly because therapeutic temperature was not reached before reperfusion in the majority of the patients. We aimed to evaluate the safety and feasibility of rapidly induced hypothermia by infusion of cold saline and endovascular cooling catheter before reperfusion in patients with acute myocardial infarction. METHODS AND RESULTS: =0.12). Despite similar duration of ischemia (174+/-51 minutes versus 174+/-62 minutes, hypothermia versus control, P=1.00), infarct size normalized to myocardium at risk was reduced by 38% in the hypothermia group compared with the control group (29.8+/-12.6% versus 48.0+/-21.6%, P=0.041). This was supported by a significant decrease in both peak and cumulative release of Troponin T in the hypothermia group (P=0.01 and P=0.03, respectively). Conclusions-The protocol demonstrates the ability to reach a core body temperature of <35 degrees C before reperfusion in all patients without delaying primary percutaneous coronary intervention and that combination hypothermia as an adjunct therapy in acute myocardial infarction may reduce infarct size at 3 days as measured by MRI. Clinical Trial Registration-URL: http://clinicaltrials.gov. Unique identifier: NCT00417638.
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| 31. |
- Holm, Åsa
(författare)
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Dose Plan Optimization in HDR Brachytherapy using Penalties : Properties and Extensions
- 2011
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- High dose-rate (HDR) brachytherapy is a specific type of radiotherapy used to treat tumours of for example the cervix, prostate, and breasts. In HDR brachytherapy applicators are implanted into or close to the tumour volume. A radioactive source is moved through these applicators and stops at certain positions, known as dwell points. For each patient an anatomy-based dose plan is created that decides for example where to place the applicators, which dwell points to use, and for how long. The aim when creating a dose plan is to deliver an as high dose as possible to the tumour while simultaneously keeping the dose to the surrounding healthy organs as low as possible.In order to improve the quality of dose plans mathematical optimization methods are today used in clinical practice. Usually one solves a linear penalty model that minimizes a weighted deviation from dose intervals provided by a physician. In this thesis we study certain properties and alterations of this model.One interesting property of the model that we study is the distribution of the basic variables. We show that due to the distribution of these variables only a limited number of dwell positions can be used. Since relatively few dwell positions are used some of the corresponding dwell times have to be long in order for the desired overall dose level to be reached. These long dwell times have been observed in clinical practice and are considered to be a problem.Another property that we study is the correlation between the objective value of the linear penalty model and dose-volume parameters used for evaluation of dose plans. We show that the correlation is weak, which implies that optimizing the linear penalty model does not give a solution to the correct problem.Some alternative models are also considered. One that includes into the optimization the decision of where to place the applicators, when HDR brachytherapy is applied for prostate cancer, and one that reduces the long dwell times by using piecewise linear penalties. The solutions to both models show significant improvements.
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| 32. |
- Inayat, S, et al.
(författare)
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High levels of cathepsins B, L and S in human seminal plasma and their association with prostasomes
- 2012
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Ingår i: Andrologia. - : Hindawi Limited. - 0303-4569 .- 1439-0272. ; 44:6, s. 423-427
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Tidskriftsartikel (refereegranskat)abstract
- Semen is a heterogenous and complex fluid with different functions, some of them well known, others still obscure. The aim of this study was to investigate the presence of cathepsins B, S and L in human seminal plasma and their possible associations with other semen variables. Cathepsin B, L and S concentrations were measured in seminal plasma from 99 men utilising commercial ELISA kits. Seminal plasma cathepsin B was approximately 70 times higher, while the cathepsin L values were approximately 500 times higher and the cathepsin S values approximately 40 times higher in seminal plasma than in a group of serum samples. The study shows that seminal plasma contains high levels of cathepsins B, L and S. All three cathepsins were also bound to the surface of prostasomes.
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| 33. |
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| 34. |
- Lagerqvist, Bo, et al.
(författare)
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Outcomes 1 year after thrombus aspiration for myocardial infarction.
- 2014
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Ingår i: New England Journal of Medicine. - : Massachusetts Medical Society. - 0028-4793 .- 1533-4406. ; 371:12, s. 1111-1120
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Tidskriftsartikel (refereegranskat)abstract
- Routine intracoronary thrombus aspiration before primary percutaneous coronary intervention (PCI) in patients with ST-segment elevation myocardial infarction (STEMI) has not been proved to reduce short-term mortality. We evaluated clinical outcomes at 1 year after thrombus aspiration.
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| 35. |
- Larsson, Billy, 1956, et al.
(författare)
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Klimatfrågan kräver en ny global etik
- 2012
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Ingår i: Göteborgs Posten. - 1103-9345. ; :12 Augusti 2012
-
Tidskriftsartikel (populärvet., debatt m.m.)
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| 36. |
- Lindberg, Ulrika, et al.
(författare)
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BPI-ANCA and long-term prognosis among 46 adult CF patients: a prospective 10-year follow-up study
- 2012
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Ingår i: Clinical & Developmental Immunology. - : Hindawi Publishing Corporation. - 1740-2522 .- 1740-2530. ; :370107
-
Tidskriftsartikel (refereegranskat)abstract
- IntroductionAnti-neutrophil cytoplasmic antibodies specific for bactericidal/permeability-increasing protein (BPI-ANCA) are frequent in CF patients and mainly develop in response to infection with Pseudomonas aeruginosa. It is not known to what extent BPI-ANCA correlates to prognosis.Objectives.To evaluate the prognostic value of IgA-BPI-ANCA, measured at the beginning of the study, for transplantation-free survival.Methods.A cohort of 46 adult, nontransplanted CF patients was generated, 1995–1998, and characterized using Leeds criteria, lung function, and IgA-BPI-ANCA levels measured by ELISA. The cohort was followed until December 2009, using the combined endpoint of death or lung transplantation.Results.Lung function and IgA-BPI-ANCA, but not Leeds criteria, were significantly associated with adverse outcome. No patient with normal lung function at baseline reached endpoint. Within 10 years 8/11 with high BPI-ANCA reached an endpoint compared to 3/17 ANCA-negative patients. A similar result was seen within the Leeds I group where 7 out of 9 BPI-ANCA-positive patients reached endpoint, compared to none of the 5 patients without BPI-ANCA.Conclusions.IgA-BPI-ANCA is associated with adverse outcome among Pseudomonas aeruginosa infected CF patients, suggesting that BPI-ANCA is a biomarker of an unfavourable host-pathogen interaction
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| 37. |
- Lund, Philip E., et al.
(författare)
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Comparison of two infusion rates of antithrombin concentrate in cardiopulmonary bypass surgery
- 2010
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Ingår i: Perfusion. - : SAGE Publications. - 1477-111X .- 0267-6591. ; 25:5, s. 305-312
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Tidskriftsartikel (refereegranskat)abstract
- Background: Antithrombin concentrate (AT) is used to treat heparin resistance (HR) in cardiac surgery. It is usually given slowly due to the fear of anaphylaxis. This may delay cardiac catheterisation and the start of cardiopulmonary bypass (CPB). HR is often defined as the failure to reach or maintain a target activated clotting time (ACT) despite a standard dose of heparin. It is not generally possible to predict which patients will display HR, although there are known risk factors. Routine early administration of AT before heparinisation is probably not cost-effective. Infusing AT relatively quickly after demonstrating HR may be more cost-effective, while not delaying surgery. The aim of this study is to investigate the safety and side effects of a faster infusion of AT. Methods: Forty patients undergoing elective heart surgery were included and randomised to two groups in a double-blind fashion. Each group received 1000 IU of AT intravenously (IV). One group received a slow infusion (100 IU/min) before full-dose heparinisation. The other group received a fast infusion (250 IU/min). Haemodynamic and respiratory data were recorded. Any adverse effects were noted. Thrombin-antithrombin, anti-Xa and antithrombin levels in plasma were measured. Results: No anaphylaxis occurred in either group. No differences were found regarding haemodynamics, respiration or laboratory results. Two patients experienced major haemorrhage and recovered; there were two deaths, thought to be unrelated to the study drugs. Conclusion: AT can be infused at a rate of 250 IU/min. This is faster than the current recommendation of 100 IU/min. This rate of infusion allows restricting AT infusion to those patients who display HR, without delaying surgery. Optimal anticoagulant therapy for CPB probably includes point-of-care measurement of ACT and plasma AT and small, but rapid, infusions of AT in heparin-resistant patients.
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| 38. |
- Neovius, Martin, et al.
(författare)
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Health Care Use During 20 Years Following Bariatric Surgery
- 2012
-
Ingår i: JAMA: The Journal of the American Medical Association. - : American Medical Association (AMA). - 0098-7484 .- 1538-3598. ; 308:11, s. 1132-1141
-
Tidskriftsartikel (refereegranskat)abstract
- Context Bariatric surgery results in sustained weight loss; reduced incidence of diabetes, cardiovascular events, and cancer; and improved survival. The long-term effect on health care use is unknown. Objective To assess health care use over 20 years by obese patients treated conventionally or with bariatric surgery. Design, Setting, and Participants The Swedish Obese Subjects study is an ongoing, prospective, nonrandomized, controlled intervention study conducted in the Swedish health care system that included 2010 adults who underwent bariatric surgery and 2037 contemporaneously matched controls recruited between 1987 and 2001. Inclusion criteria were age 37 years to 60 years and body mass index of 34 or higher in men and 38 or higher in women. Exclusion criteria were identical in both groups. Interventions Of the surgery patients, 13% underwent gastric bypass, 19% gastric banding, and 68% vertical-banded gastroplasty. Controls received conventional obesity treatment. Main Outcome Measures Annual hospital days (follow-up years 1 to 20; data capture 1987-2009; median follow-up 15 years) and nonprimary care outpatient visits (years 2-20; data capture 2001-2009; median follow-up 9 years) were retrieved from the National Patient Register, and drug costs from the Prescribed Drug Register (years 7-20; data capture 2005-2011; median follow-up 6 years). Registry linkage was complete for more than 99% of patients (4044 of 4047). Mean differences were adjusted for baseline age, sex, smoking, diabetes status, body mass index, inclusion period, and (for the inpatient care analysis) hospital days the year before the index date. Results In the 20 years following their bariatric procedure, surgery patients used a total of 54 mean cumulative hospital days compared with 40 used by those in the control group (adjusted difference, 15; 95% CI, 2-27; P = .03). During the years 2 through 6, surgery patients had an accumulated annual mean of 1.7 hospital days vs 1.2 days among control patients (adjusted difference, 0.5; 95% CI, 0.2 to 0.7; P < .001). From year 7 to 20, both groups had a mean annual 1.8 hospital days (adjusted difference, 0.0; 95% CI, −0.3 to 0.3; P = .95). Surgery patients had a mean annual 1.3 nonprimary care outpatient visits during the years 2 through 6 vs 1.1 among the controls (adjusted difference, 0.3; 95% CI, 0.1 to 0.4; P = .003), but from year 7, the 2 groups did not differ (1.8 vs 1.9 mean annual visits; adjusted difference, −0.2; 95% CI, −0.4 to 0.1; P = .12). From year 7 to 20, the surgery group incurred a mean annual drug cost of US $930; the control patients, $1123 (adjusted difference, −$228; 95% CI, −$335 to −$121; P < .001). Conclusions Compared with controls, surgically treated patients used more inpatient and nonprimary outpatient care during the first 6-year period after undergoing bariatric surgery but not thereafter. Drug costs from years 7 through 20 were lower for surgery patients than for control patients.
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| 39. |
- Odin, Elisabeth, 1955, et al.
(författare)
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Determination of reduced folates in tumor and adjacent mucosa of colorectal cancer patients using LC-MS/MS.
- 2013
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Ingår i: Biomedical chromatography : BMC. - : Wiley. - 1099-0801 .- 0269-3879. ; 27:4, s. 487-495
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Tidskriftsartikel (refereegranskat)abstract
- A liquid chromatography electrospray ionization tandem mass spectrometry (LC-MS/MS) method has been developed for the determination of 5,10-methylenetetrahydrofolate (methyleneTHF), tetrahydrofolate (THF) and 5-methyltetrahydrofolate (methylTHF) in colorectal mucosa and tumor tissues. The folate extraction method includes homogenization, heat and folate conjugase treatment to hydrolyze polyglutamyl folate to monoglutamyl folate. Before analysis on LC-MS/MS, simple and fast sample purification with ultrafiltration (molecular weight cut-off membrane, 10kDa) was performed. Folates were detected and quantified using positive electrospray. The method described in the present paper was successfully applied to determine the level of three folate monoglutamates in mucosa and tumor samples from 77 colorectal cancer patients, starting from a limited amount of tissue. The results showed that the LC-MS/MS method has a great advantage over other previously used methods because of its high sensitivity and selectivity. Significantly higher levels of methyleneTHF and THF were found in tumor compared with matched mucosa tissues. Folate levels in adjacent mucosa were associated with tumor location, age and gender. The correlation between folate levels and tumor site further strengthens the fact that development of right- and left-sided tumors follows different pathways. Copyright © 2012 John Wiley & Sons, Ltd.
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| 40. |
- Pettersson, Karin, et al.
(författare)
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Cost identification of Nordic FLIRI, Nordic FLOX, XELIRI and XELOX in first-line treatment of advanced colorectal cancer in Sweden : A clinical practice model approach
- 2012
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Ingår i: Acta Oncologica. - 0284-186X .- 1651-226X. ; 51:7, s. 840-848
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Tidskriftsartikel (refereegranskat)abstract
- Introduction. The role of health-related economy is crucial due to the finite healthcare resources. Intravenous (i.v.) regimes Nordic FLOX and Nordic FLIRI, and the partly oral alternatives XELIRI and XELOX are four commonly used chemotherapies in the first-line treatment of advanced colorectal cancer (CRC) in the Scandinavian countries, all with different costs. Aim. To describe and compare costs associated with four commonly used treatments for advanced CRC in clinical routine practice. An additional aim was to evaluate the theoretical cost impact of adverse effects associated with the therapies. Material and methods. The retrospective study was carried out using observations and a clinical quality database of CRC patients treated with Nordic FLOX, Nordic FLIRI, XELIRI and XELOX as first line at an oncology clinic in Gothenburg, Sweden. The treatments are used in parallel in clinical practice. All patients treated from 2003 to 2009 were included. The clinical outcome of the therapies was equivalent; mean treatment time was 5.9-7.7 months. A clinical economic evaluation model was designed. All direct costs associated with the baseline treatment, administration of chemotherapy and drug costs were collected and evaluated. Results. The maximum cost for the four treatments was estimated to be 72 000-75 000 SEK per patient for six months, of this approximately 8000 SEK was linked to treatment of toxicity. During six months the i.v. treatments could include 17 more outpatient visits per patient compared to the oral alternatives. During treatment at the clinic around 20% of the patients were hospitalised (XELOX excluded, because of few included patients). Conclusion. The results indicate that the four regimens are similar in terms of treatment costs. Different costs affect the total cost. The oral alternative makes it possible to treat additional patients with the same labour force resources. Treatment of adverse effects contributes to extensive resource use at the hospital.
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| 41. |
- Ronquist, Göran K, et al.
(författare)
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Biochemical characterization of stallion prostasomes and comparison to their human counterparts
- 2013
-
Ingår i: Systems biology in reproductive medicine. - : Informa UK Limited. - 1939-6376 .- 1939-6368. ; 59:6, s. 297-303
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Tidskriftsartikel (refereegranskat)abstract
- Release of nanometer-sized prostasomes into human and equine semen suggests essential functions in their relationships with sperm cells and the fertilization process. The two types of prostasomes displayed ultrastructural similarities, albeit the human prostasomes were somewhat larger than the stallion prostasomes. A high ratio of saturated fatty acids was characteristic for the two prostasome types. Electrophoretic separation systems revealed an equine prostasomal pattern different from that of human. The 21 distinctive low molecular weight protein spots in the 2D-gel (with no counterparts in human prostasomes) were identified via peptide mass fingerprinting, several of which may be different isoforms. Out of the three high molecular weight bands characteristic for human prostasomes (CD10, CD13, and CD26), CD10 and CD13 were retrieved in equine prostasomes. We present some new proteins of horse prostasomes not found in their human counterparts. Further studies are warranted to reveal the function of these proteins.
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| 42. |
- Ronquist, Göran K., et al.
(författare)
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Prostasomal DNA Characterization and Transfer Into Human Sperm
- 2011
-
Ingår i: Molecular Reproduction and Development. - : Wiley. - 1040-452X .- 1098-2795. ; 78:7, s. 467-476
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Tidskriftsartikel (refereegranskat)abstract
- Human prostasomes, exosome-like microvesicles secreted by acinar cells of the prostate gland, contain chromosomal DNA. Agarose gel electrophoresis of DNA from seminal prostasomes displayed fragments of over 12 kb and smaller, with a distinct band around 1 kb that was excised, cloned, and sequenced. The sequences showed 8 out of 25 clones (32%) originating from genes. We elaborated the concept further by carrying out a genome-wide DNA copy number analysis of prostasomal DNA, hypothesizing that human prostasomes contain fragments of DNA randomly selected from the entire genome. Acridine orange-stained prostasomes were incubated with freshly prepared sperm for different times, and a transfer of acridine orange-stained prostasomal DNA to sperm (preferentially the head region) was observed. Fluorescence microscopy of slices in the center of 14 optical slides of the sperm head displayed an even fluorescence rather than a halo-like one, indicating DNA-uptake rather than just binding along the sperm head membrane.
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| 43. |
- Ronquist, Göran, et al.
(författare)
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Proteomic analysis of prostate cancer metastasis : derived prostasomes
- 2010
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Ingår i: Anticancer Research. - 0250-7005 .- 1791-7530. ; 30:2, s. 285-290
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Tidskriftsartikel (refereegranskat)abstract
- The secretory epithelial cells of the prostate gland use sophisticated vehicles in the form of prostasomes to relay important information to sperm cells in semen. This prostasome-forming and secretory ability of the epithelial cells is also preserved in poorly differentiated prostate cancer cells. We investigated prostasomes from vertebral metastases of prostate cancer, taken from the operating field at surgery, directly taken care of under protease inhibitory conditions for later 2-dimensional electrophoresis and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) protein characterization. A total of 104 spots were punched out for identification. Twenty five unique protein spots had a MALDI-TOF above 49 and another 5 proteins were determined by MS/MS. The remaining 74 spots were either identical to already determined proteins or had no reliable score. Annexins A1, A3, and A5 as well as dimethylarginine dimethylaminohydrolase 1 were among the identified proteins. The annexins and dimethylarginine dimethylaminohydrolase 1 found in cancer-derived prostasomes can act, among other things, as angiogenic factors and can increase the vascular development in the neighborhood of the tumor. Cancer-derived prostasomes may play an important role in the interaction between tumor cells and their environment.
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| 44. |
- Sahlén, Göran, et al.
(författare)
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Secretions from seminal vesicles lack characteristic markers for prostasomes
- 2010
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Ingår i: Upsala Journal of Medical Sciences. - : Uppsala Medical Society. - 0300-9734 .- 2000-1967. ; 115:2, s. 107-112
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Tidskriftsartikel (refereegranskat)abstract
- Background. Prostasomes are suggested to be produced in the prostate gland. Although biochemical studies support this, some immunohistochemical findings indicate that also the seminal vesicles could be a source of prostasomes. Therefore, we have compared the secretion of the vesicles with that of the prostate using biochemical and ultrastructural techniques. Methods. Ultracentrifuged pellets of substance from seminal vesicle secretions were analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and flow cytometry. The secretory cells of the seminal vesicles were examined with transmission electron microscopy. These findings were then compared with published results from similar studies of the prostate secretory cells. Results. In SDS-PAGE, the seminal vesicle pellets lacked the three prostasome-characteristic CD-markers, namely CD10, CD13, and CD26, but expressed two proteins of about 55 kDa and 70 kDa, corresponding to clusterin and heat shock protein (HSP70). Flow cytometry showed the presence of secretion particles in the seminal pellet, although of a smaller size than that of the prostasomes. Electron microscopy of the luminal part of the cells in the seminal vesicles demonstrated many secretion granules, each enclosed in a vesicle with a size of about 1 mum. Conclusions. Pelleted seminal vesicle secretion is different to prostate secretion in several ways. No prostasome characteristics were detected in the pelleted seminal vesicle secretion.
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| 45. |
- Sandgren, Buster, et al.
(författare)
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Computed Tomography vs. Digital Radiography Assessment for Detection of Osteolysis in Asymptomatic Patients With Uncemented Cups: A Proposal for a New Classification System Based on Computer Tomography.
- 2013
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Ingår i: The Journal of arthroplasty. - : Elsevier BV. - 1532-8406 .- 0883-5403. ; 28:9, s. 1608-1613
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Tidskriftsartikel (refereegranskat)abstract
- Digital radiographic images in the anterior-posterior and lateral view have been gold standard for evaluation of peri-acetabular osteolysis for patients with an uncemented hip replacement. We compared digital radiographic images and computer tomography in detection of peri-acetabular osteolysis and devised a classification system based on computer tomography. Digital radiographs were compared with computer tomography on 206 hips, with a mean follow up 10years after surgery. The patients had no clinical signs of osteolysis and none were planned for revision surgery. On digital radiographs, 192 cases had no osteolysis and only 14 cases had osteolysis. When using computer tomography there were 184 cases showing small or large osteolysis and only 22 patients had no osteolysis. A classification system for peri-acetabular osteolysis is proposed based on computer tomography that is easy to use on standard follow up evaluation.
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| 46. |
- Sarno, Giovanna, et al.
(författare)
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Initial clinical experience with an everolimus eluting platinum chromium stent (Promus Element) in unselected patients from the Swedish Coronary Angiography and Angioplasty Registry (SCAAR)
- 2013
-
Ingår i: International Journal of Cardiology. - : Elsevier BV. - 0167-5273 .- 1874-1754. ; 167:1, s. 146-150
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Tidskriftsartikel (refereegranskat)abstract
- Background: The safety and efficacy of the Promus Element stent have been recently demonstrated in a selected population from one randomized trial. The aim of this study was to describe the initial clinical experience with the everolimus eluting platinum chromium stent (Promus Element) in unselected patients from a real life nationwide registry.Methods: The Promus Element DES was compared to all other DES implanted in Sweden (with more than 500 implants) from November 2009 to March 2011. The results were assessed using Cox regression.Results: A total of 13,577 stents (Promus Element, n = 2724, Cypher, n = 782; Endeavor, n = 747; Taxus Liberte, n = 1393, Xience V/Promus, n = 4832, Resolute, n = 1566, Xience Prime, n = 4832) were implanted at 8375 procedures. At one year the restenosis rate in the Promus Element was not significantly different from the overall DES group (2.8% vs. 2.7%, adjusted HR: 1.17, 95% CI: 0.75-1.75). A significantly lower restenosis rate was observed in the Promus Element when compared with Endeavor (2.8% vs. 5.8%; adjusted HR: 0.44; 95% CI: 0.26-0.74). The stent thrombosis (ST) rate at one year was not significantly different in the Promus Element as compared with the overall DES group (0.2% vs. 0.5% adjusted HR: 0.59; 95% CI: 025-1.40). ST rate was significantly lower as compared with Endeavor stent (0.2% vs. 0.8%; HR: 0.24; 95% CI: 0.08-0.67).Conclusions: In a large unselected population the Promus Element stent appears to be safe and effective with a low risk of restenosis and ST. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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| 47. |
- Strand, Mårten, 1982-, et al.
(författare)
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Isolation and characterization of anti-adenoviral secondary metabolites from marine actinobacteria
- 2014
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Ingår i: Marine Drugs. - : MDPI. - 1660-3397. ; 12:2, s. 799-821
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Tidskriftsartikel (refereegranskat)abstract
- Adenovirus infections in immunocompromised patients are associated with high mortality rates. Currently, there are no effective anti-adenoviral therapies available. It is well known that actinobacteria can produce secondary metabolites that are attractive in drug discovery due to their structural diversity and their evolved interaction with biomolecules. Here, we have established an extract library derived from actinobacteria isolated from Vestfjorden, Norway, and performed a screening campaign to discover anti-adenoviral compounds. One extract with anti-adenoviral activity was found to contain a diastereomeric 1:1 mixture of the butenolide secondary alcohols 1a and 1b. By further cultivation and analysis, we could isolate 1a and 1b in different diastereomeric ratio. In addition, three more anti-adenoviral butenolides 2, 3 and 4 with differences in their side-chains were isolated. In this study, the anti-adenoviral activity of these compounds was characterized and substantial differences in the cytotoxic potential between the butenolide analogs were observed. The most potent butenolide analog 3 displayed an EC50 value of 91 μM and no prominent cytotoxicity at 2 mM. Furthermore, we propose a biosynthetic pathway for these compounds based on their relative time of appearance and structure.
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| 48. |
- Taflin, Helena, et al.
(författare)
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Folate Levels and Polymorphisms in the Genes MTHFR, MTR, and TS in Colorectal Cancer.
- 2014
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Ingår i: Clinical Medicine Insights. Oncology. - 1179-5549. ; 8, s. 15-20
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to explore and describe the effect of polymorphisms in folate-associated genes regarding the levels of different folate forms and their distribution in tumors and mucosa in patients with colorectal cancer.
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| 49. |
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| 50. |
- Toller, Susanna, et al.
(författare)
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Energy Use and Environmental impacts of the Swedish Building and Real Estate Management Sector
- 2011
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Ingår i: Journal of Industrial Ecology. - : Wiley. - 1088-1980 .- 1530-9290. ; 15:3, s. 394-404
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Tidskriftsartikel (refereegranskat)abstract
- One of the key features of environmental policy integration in Sweden is sectorresponsibility. The National Board of Housing, Building and Planning is responsible for the building and real estate management sector and should, as a part of this responsibility, assess the environmental impacts of this sector. The aim of this study is to suggest and demonstrate a method for such an assessment. The suggested method is a life cycle assessment, based on an input-output analysis. The method can be used for regular monitoring and for prioritization between different improving measures. For the assessment to sufficiently cover the Swedish Environmental Quality Objectives, complementary information is needed, in particular with respect to the indoor environment. According to the results, the real estate management sector contributes between 10% and 40% of Swedish energy use; use of hazardous chemical products; generation of solid waste; emissions of gases contributing to climate change; and human toxicological impacts, including nitrogen oxides (NOx) and particulates. Transport and production of nonrenewable building materials contribute significantly to several of the emissions. Heating of buildings contributes more to energy use than to climate change, due to the use of renewable energy sources. To reduce climate change, measures should therefore prioritize not only heating of buildings but also the important upstream processes.
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