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- Olsson, P., et al.
(författare)
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Uptake, toxicity and radiation effects of the boron compounds DAAC-1 and DAC-1 in cultured human glioma cells
- 1998
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Ingår i: International Journal of Radiation Biology. - 0955-3002 .- 1362-3095. ; 73:1, s. 103-112
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Tidskriftsartikel (refereegranskat)abstract
- PURPOSE: To study the uptake, toxicity and radiation effects in vitro of a diol-amino acid-carborane (DAAC-1) and make comparisons with the previously studied diol-amine-carborane (DAC-1). MATERIALS AND METHODS: Toxicity and radiation effects were studied with clonogenic survival, uptake by measuring the cellular boron content and the subcellular distribution was investigated after organelle separation with centrifugation. The studied cell line was human glioma U343. RESULTS: DAAC-1 showed an accumulation of 1-1.5 times, compared with the culture medium, and was non-toxic up to 47 microg boron/ml. The accumulation of DAC-1 was about 90 times, but toxic effects were detectable already at the concentration 5 microg boron/ml. None of the compounds was localized in the cell nucleus. Following irradiation with thermal neutrons, DAC-1 was about 2.5 times more effective than DAAC-1 and about 4.9 times more effective than neutrons alone, at the survival level 0.2. The dose modifying factors, when compared with the neutron beam alone, were for both DAAC-1 and DAC-1 about 1.5 and about 5 when compared with 60Co-gamma-radiation. CONCLUSIONS: DAAC-1 was less toxic than DAC-1 but gave less accumulation of boron. Both substances gave significant boron-dependent cell inactivation when the test cells were exposed to thermal neutrons.
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- Sandell, Rolf, et al.
(författare)
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As time goes by. Long-term outcomes of psychoanalysis and long-term psychotherapy
- 1999
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Ingår i: Forum der Psychoanalyse (Print). - 0178-7667 .- 1437-0751. ; 15:4, s. 327-347
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Tidskriftsartikel (refereegranskat)abstract
- Generalizations about the outcomes of psychotherapy are essentially based on research on brief therapies with quite short follow-up. The present paper is a survey of some of the findings in an effectiveness study of psychoanalysis and long-term psychotherapy, typically psychoanalytically oriented, in private practice. The design was a three-wave panel comprising more than 700 patients. At the end of the third wave, 418 patients in different phases of treatment remained in the sample. Across a reconstructed time scale covering roughly seven years, before, during, and after treatment, the 74 psychoanalysis cases described a very positive development, whereas the development among the 331 psychotherapy cases was moderately positive. The findings emphasize the importance of extended follow-up. When therapist variables are considered, therapeutic experience had complex or mixed associations with patients outcome. Especially positive were high age and many years, in private practice, after licensing. Therapeutic ideals and attitudes divided the therapist group in three subgroups, one of which was characterized by purely classical psychoanalytic views on the values of support, friendliness etc. Therapists in the classically psychoanalytic cluster were found to do poorly with patients in psychotherapy, in contrast to patients in psychoanalysis. It is concluded that psychoanalysis and psychodynamically oriented psychotherapy are qualitatively different.
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- Carlsson, A., et al.
(författare)
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Prevalence of coeliac disease in Turner syndrome
- 1999
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Ingår i: Acta Pædiatrica. - 1651-2227 .- 0803-5253. ; 88, s. 933-
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Tidskriftsartikel (refereegranskat)abstract
- This study was undertaken to investigate the prevalence of coeliac disease in children and adolescents with Turner syndrome. Eighty-seven children and adolescents with Turner syndrome were screened for IgA- antiendomysium antibodies (EMA) and IgA-antigliadin antibodies (AGA), 5% (4/87) being found to be EMA-positive, and 15% (13/87) to have AGA levels above normal. Of the 10 patients who were either AGA- or EMA-positive and further investigated with intestinal biopsy, four manifested villous atrophy (i.e. all three of the EMA-positive patients, but only one of the seven AGA- positive patients). The results suggest EMA-positivity to be a good immunological marker for use in screening for coeliac disease, and such screening to be justified in patients with Turner syndrome.
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- Bjarnason, R, et al.
(författare)
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Leptin levels are strongly correlated with those of GH-binding protein in prepubertal children.
- 1997
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Ingår i: European Journal of Endocrinology. - 0804-4643 .- 1479-683X. ; 137:1, s. 68-73
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Tidskriftsartikel (refereegranskat)abstract
- There was a highly significant correlation between serum levels of leptin and those of GHBP, except in children with GHD. The possibility that leptin could mediate the effects of body fat mass on GH sensitivity, therefore, merits further investigation.
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- Carlsson, Christina, 1968, et al.
(författare)
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Screening for genetic mutations
- 1996
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 380:6571, s. 207-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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- Carlsson, J., et al.
(författare)
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Microsatellites reveal fine-scale genetic structure in stream-living brown trout
- 1999
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Ingår i: Journal of Fish Biology. - : John Wiley & Sons. - 0022-1112 .- 1095-8649. ; 55:6, s. 1290-1303
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Tidskriftsartikel (refereegranskat)abstract
- Multilocus F(ST) estimates revealed a pronounced genetic structure at six microsatellite loci in brown trout Salmo trutta in Nordre Finnvikelv, with at least three breeding units that remained stable over time. Significant differences in allele frequencies were found between five sections within a 3-km range, even when no physical barriers prevented fish from migrating between sections. It is argued that geological structures may rise to patterns resembling isolation by distance. Seemingly, the most important factor causing genetic differentiation in Nordre Finnvikelv is genetic drift in small populations that are geologically subdivided by a tributary and by impassable waterfalls. Some correlation between previous behavioural observations and genetic structures were found.
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- Gronvik, C, et al.
(författare)
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The non-variation in radiosensitivity of different proliferative states of human glioma cells
- 1996
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Ingår i: ANTICANCER RESEARCH. - : INT INST ANTICANCER RESEARCH. - 0250-7005. ; 16:1, s. 25-31
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The radiosensitivity of the human glioma cell line U-343MGa, while growing as spheroids and as conventional monolayers, was studied. The spheroids were first irradiated with Co-60 photons, and the radiosensitivity was then analysed in different cell layer
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- Hagelin, J., et al.
(författare)
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Swedes and animal tests
- 1999
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Ingår i: New scientist (1971). - 0262-4079. ; 163
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Tidskriftsartikel (refereegranskat)
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| 31. |
- J. Carlsson, J.-C. Janson and M. Sparrman
(författare)
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Affinity Chromatography
- 1998
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Ingår i: J.-C. Janson and L. Rydén, (eds.), Protein Purification, Principles, High Resolution Methods and Applications. - : John Wiley & Sons Inc., New York. ; , s. 375-443
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 42. |
- Zhao, Q, et al.
(författare)
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Conjugate chemistry, iodination and cellular binding of mEGF-dextran-tyrosine: preclinical tests in preparation for clinicaltrials
- 1998
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Ingår i: International Journal of Molecular Medicine. - : Spandidos Publications. - 1107-3756 .- 1791-244X. ; 1:4, s. 693-702
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Tidskriftsartikel (refereegranskat)abstract
- A conjugate with specific binding to the epidermal growth factor receptor, EGFR, and of interest for clinical tests was prepared using mouse epidermal growth factor, mEGF, and dextran. The mEGF was first coupled to dextran by reductive amination in which the free amino group on the N-terminal of mEGF was reacted with the aldehyde group on the reductive end of the dextran chain. The end-end coupled intermediate was further activated by the cyanopyridinium agent CDAP and tyrosines introduced to the dextran part of the conjugate. The mEGF-dextran-tyrosine conjugate was, with high efficiency, iodinated with the chloramine-T method. Approximately 25-35% of the radioactivity could be removed from the conjugate after exposure to protease K while 65-75% of the radioactivity could be removed after exposure to dextranase. Thus, the largest amount of the iodine was on the dextran part of the conjugate. The iodinated mEGF-dextran-tyrosine had EGFR specific binding since the binding to an EGFR rich human glioma cell line could be displaced by an excess of non-radioactive mEGF. The conjugate was to a large extent internalized in these cells and the administrated radioactivity was thereby retained inside the cells for at least up to 50 h.
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| 43. |
- Zhao, QH, et al.
(författare)
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Effects of dextranation on the pharmacokinetics of short peptides. A PET study on mEGF
- 1999
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Ingår i: BIOCONJUGATE CHEMISTRY. - : AMER CHEMICAL SOC. - 1043-1802. ; 10:6, s. 938-946
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The effects of dextranation on the biodistribution of mouse epidermal growth factor (mEGF, 6 kDa) were assessed. By reductive amination, mEGF was coupled to 13 and 46 kDa dextran. The two dextranated conjugates and free mEGF were labeled with the positron
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| 46. |
- Anderson, Leif G., et al.
(författare)
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The effect of the Siberian tundra on the environment of the shelf seas and the Arctic Ocean
- 1999
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Ingår i: Ambio. - 0044-7447. ; 28:3, s. 270-280
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Tidskriftsartikel (refereegranskat)abstract
- The Tundra Ecology -94 expedition investigated inflow of inorganic and organic carbon to the shelf seas by river runoff, and its transformation by biochemical processes in seawater and sediment. In addition, anthropogenic radionuclides, 137Cs, 90Sr, and 239,240Pu, were studied in water and sediments. The distribution of dissolved inorganic carbon indicates that the majority of the Ob and Yenisey discharges flow into the Laptev Sea before entering the central Arctic Ocean. The sediment study shows that there is a marked difference in benthic oxygen uptake, efflux of dissolved inorganic carbon and nutrients between localities. 137Cs activity from the Chernobyl accident is 30% in the Barents, Kara, and Laptev Seas. 137Cs increased from 5-8 Bq m-3 in Barents Sea, 5-13 Bq m-3 in the Kara Sea to 8-15 Bq m-3 in the Laptev Sea, but with locally low concentrations at the river mouths. Corresponding values for 90Sr were 2.5, 3, and 4 Bq m-3, respectively.
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| 50. |
- Bang, P., et al.
(författare)
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Postoperative induction of insulin-like growth factor binding protein-3 proteolytic activity : relation to insulin and insulin sensitivity
- 1998
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Ingår i: Journal of Clinical Endocrinology and Metabolism. - Cary, NC, USA : Oxford University Press. - 0021-972X .- 1945-7197. ; 83:7, s. 2509-2515
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Tidskriftsartikel (refereegranskat)abstract
- Increased serum insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) proteolytic activity (IGFBP-3-PA) has been demonstrated in a number of clinical states of insulin resistance, including severe illness, after surgery, and in noninsulin-dependent diabetes mellitus. In the present study we assessed the role of insulin sensitivity in expression of IGFBP-3-PA in serum. In 18 patients studied, a significant increase in IGFBP-3-PA (P < 0.005) was demonstrated after cole-rectal surgery. Eight patients receiving an oral glucose load before surgery demonstrated a significant greater relative increase in IGFBP-3-PA compared with 10 patients not receiving glucose (32.9 +/- 7.1% vs. 8.6 +/- 6.7%, respectively; P < 0.05). Both groups had reduced insulin sensitivity after surgery(-58 +/- 4%; P < 0.0001; n = 18), as determined by hyperinsulinemic, normoglycemic clamps; however, the group not receiving glucose displayed 18% less insulin sensitivity than the oral glucose load group (P < 0.05). Multiple regression analysis demonstrated that the relative changes in IGFBP-3-PA and C peptide levels were inversely correlated (P < 0.05), suggesting that increased IGFBP-3-PA, presumably increasing IGF bioavailability, may be associated with decreased insulin demands. Interestingly, insulin infusion during the 4-h hyperinsulinemic, normoglycemic clamp performed 24 h after surgery (post-op) resulted in a further increase in IGFBP-3-PA in both groups (P < 0.005), whereas no significant responses could be demonstrated during the pre-op clamp. The expression of increased IGFBP-3-PA was accompanied by conversion of endogenous intact 39/42-kDa IGFBP-3 into its 30-kDa fragmented form as determined by Western immunoblotting, and this conversion was virtually complete after the 4-h post-op clamp in patients displaying marked increases in IGFBP-3-PA. Characterization of the IGFBP-3-PA demonstrated that it was specific for IGFBP-3, as no degradation of IGFBP-1 and -2 was detected, and the use of various protease inhibitors demonstrated that serine proteases and possibly matrix metalloproteinases contribute to the increased IGFBP-3-PA level after surgery. We propose that IGF bioavailability may be increased by the induction of IGFBP-3-PA in insulin-resistant subjects, and that insulin regulates IGFBP-3-PA in this state.
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