| 1. |
- van der Brug, MP, et al.
(författare)
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RNA binding activity of the recessive parkinsonism protein DJ-1 supports involvement in multiple cellular pathways
- 2008
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 1091-6490. ; 105:29, s. 10244-10249
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Tidskriftsartikel (refereegranskat)abstract
- Parkinson's disease (PD) is a major neurodegenerative condition with several rare Mendelian forms. Oxidative stress and mitochondrial function have been implicated in the pathogenesis of PD but the molecular mechanisms involved in the degeneration of neurons remain unclear. DJ-1 mutations are one cause of recessive parkinsonism, but this gene is also reported to be involved in cancer by promoting Ras signaling and suppressing PTEN-induced apoptosis. The specific function of DJ-1 is unknown, although it is responsive to oxidative stress and may play a role in the maintenance of mitochondria. Here, we show, using four independent methods, that DJ-1 associates with RNA targets in cells and the brain, including mitochondrial genes, genes involved in glutathione metabolism, and members of the PTEN/PI3K cascade. Pathogenic recessive mutants are deficient in this activity. We show that DJ-1 is sufficient for RNA binding at nanomolar concentrations. Further, we show that DJ-1 binds RNA but dissociates after oxidative stress. These data implicate a single mechanism for the pleiotropic effects of DJ-1 in different model systems, namely that the protein binds multiple RNA targets in an oxidation-dependent manner.
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| 2. |
- Mathews, T.S, et al.
(författare)
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Functional reliability analysis of Safety Grade Decay Heat Removal System of Indian 500 MWe PFBR
- 2008
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Ingår i: Nuclear Engineering and Design. - : Elsevier BV. - 0029-5493 .- 1872-759X. ; 238:9, s. 2369-2376
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Tidskriftsartikel (refereegranskat)abstract
- Passive systems are increasingly deployed in nuclear industry with an objective of increasing reliability and safety of operations with reduced cost. Methods for assessing the reliability of thermal-hydraulic passive systems, that is systems with moving working fluid, address the issues in natural buoyancy-driven flow that could result in a failure to meet the design safety limits under accident scenarios. This is referred as design functional reliability. This paper presents the results of functional reliability analysis carried out for the passive Safety Grade Decay Heat Removal System (SGDHRS) of IndianPrototype Fast Breeder Reactor (PFBR). The analysis is carried out based on the overall approach reported in the Reliability Methods for Passive System (RMPS, European Commission) project. Functionalfailure probability is calculated using Monte-Carlo method and also with method of moments.
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| 3. |
- Ramakrishnan, M., et al.
(författare)
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Estimation of station blackout frequency for Indian fast breeder test reactor
- 2008
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Ingår i: Annals of Nuclear Energy. - : Elsevier BV. - 0306-4549. ; 35:12, s. 2332-2337
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Tidskriftsartikel (refereegranskat)abstract
- This paper presents the comparison of station blackout (SBO) frequency computed with approximate time averaged expressions for diesel generator unavailability and time dependent cutset evaluation method. It is found that the frequency of SBO is under predicted by a factor of 2 by approximate time averaged expressions for SBO durations of 8 h and 16 h. The time dependent cutset evaluation method is applied for offsite power feeder outage management by treating the change in SBO frequency when one of the feeders is taken out for maintenance for ‘n’ days, as the risk measure.
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