| 1. |
- Adcox, K, et al.
(författare)
-
PHENIX detector overview
- 2003
-
Ingår i: Nuclear Instruments & Methods in Physics Research. Section A: Accelerators, Spectrometers, Detectors, and Associated Equipment. - 0167-5087. ; 499:2-3, s. 469-479
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Tidskriftsartikel (refereegranskat)abstract
- The PHENIX detector is designed to perform a broad study of A-A, p-A, and p-p collisions to investigate nuclear matter under extreme conditions. A wide variety of probes, sensitive to all timescales, are used to study systematic variations with species and energy as well as to measure the spin structure of the nucleon. Designing for the needs of the heavy-ion and polarized-proton programs has produced a detector with unparalleled capabilities. PHENIX measures electron and muon pairs, photons, and hadrons with excellent energy and momentum resolution. The detector consists of a large number of subsystems that are discussed in other papers in this volume. The overall design parameters of the detector are presented. (C) 2002 Elsevier Science B.V. All rights reserved.
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| 2. |
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| 3. |
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| 4. |
- Ideguchi, E., et al.
(författare)
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Orbifold projection in supersymmetric QCD at N(f) ≤ N(c)
- 2000
-
Ingår i: Physics Letters, Section B: Nuclear, Elementary Particle and High-Energy Physics. - 0370-2693. ; 492:3-4, s. 369-375
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Tidskriftsartikel (refereegranskat)abstract
- Supersymmetric orbifold projection of N = 1 SQCD with relatively small number of flavors (N(f) ≤ N(c)) is considered. The purpose is to check whether orbifolding commutes with the infrared limit. On the one hand, one considers the orbifold projection of SQCD and obtains the low-energy description of the resulting theory. On the other hand, one starts with the low-energy effective theory of the original SQCD, and only then performs orbifolding. It is shown that at finite N(c) the two low-energy theories obtained in these ways are different. However, in the case of stabilized run-away vacuum these two theories are shown to coincide in the large N(c) limit. In the case of quantum modified moduli space, topological solitons carrying baryonic charges are present in the orbifolded low-energy theory. These solitons may restore the correspondence between the two theories provided that the soliton mass tends to zero in the large N(c) limit. (C) 2000 Elsevier Science B.V.
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| 5. |
- Pfohl, J., et al.
(författare)
-
Highly deformed rotational structures in 136Pm
- 2000
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Ingår i: Physical Review C - Nuclear Physics. - 0556-2813. ; 62:3, s. 313041-313045
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Tidskriftsartikel (refereegranskat)abstract
- Four highly deformed structures in the odd-odd nucleus 13661Pm75 were observed via the 105Pd(35Cl,2p2n) reaction at 180 and 173 MeV using the GAMMASPHERE γ-ray spectrometer and the Microball charged-particle detector array. Quadrupole moment measurements were performed on all of the bands. In contrast to lighter odd-Ζ Pm and Pr nuclei, bands based on the g9/2[404]9/2 proton orbital were not observed. Instead, the four observed sequences are assigned as a coupling of an i13/2 neutron with the low-Ω h11/2 and mixed d5/2g7/2 orbitals. Comparisons with neighboring highly deformed structures are discussed and cranked Nilsson-Strutinsky calculations for 136Pm are presented.
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| 6. |
- Karlgren, Daniel, et al.
(författare)
-
High-angular-momentum structures in Zn-64
- 2004
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Ingår i: Physical Review C. Nuclear Physics. - 0556-2813 .- 1089-490X. ; 69, s. 1-034330
-
Tidskriftsartikel (refereegranskat)abstract
- High-angular-momentum states in Zn-64 were populated in the Ca-40(Si-28,4p) reaction at a beam energy of 122 MeV. Evaporated, light, charged particles were identified by the Microball, while gamma rays were detected using the Gammasphere array. The main focus of this paper is on two strongly coupled, collective bands. The yrast band, which was previously known, has been linked to lower-lying states establishing the excitation energies and angular momenta of in-band states for the first time. The newly identified excited band decays to the yrast band but firm angular-momentum assignments could not be made. In order to interpret these structures cranked-Nilsson-Strutinsky calculations have been performed. The calculations have been extended to account for the distribution of nucleons within a configuration. The yrast collective band is interpreted as based on the pi(f(7/2))(-1)(p(3/2)f(5/2))(2)(g(9/2))(1)nu(p(3/2)f(5/2))(4)(g(9/2))(2) configuration. There are several possible interpretations of the second band but it is difficult to distinguish between the different possibilities.
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| 7. |
- Svensson, C. E., et al.
(författare)
-
Collective rotational motion in the N=Z nucleus 36Ar
- 2001
-
Ingår i: Nuclear Physics A. - 0375-9474. ; 682:1-4, s. 1-11
-
Tidskriftsartikel (refereegranskat)abstract
- A superdeformed rotational band has been identified in the N = Z nucleus 36Ar, firmly linked to known low-spin states, and observed to its high-spin termination at Iπ = 16+. Lifetime measurements by the Doppler shift attenuation method establish a large low-spin deformation (β2 ≈ 0.46) and a decrease in the collectivity as the band approaches termination. Comparisons with cranked Nilsson-Strutinsky and large-scale spherical shell model calculations lead to a consistent description of the band based on a configuration in which four particles are promoted to the pf shell. With two major shells active for both protons and neutrons, yet a valence space dimension small enough to be approached from the shell model perspective, this band offers an excellent opportunity to investigate the microscopic structure of collective rotational motion in nuclei.
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| 8. |
- Svensson, C. E., et al.
(författare)
-
Lifetimes of superdeformed rotational states in 36Ar
- 2001
-
Ingår i: Physical Review C - Nuclear Physics. - 0556-2813. ; 63:6, s. 613011-613015
-
Tidskriftsartikel (refereegranskat)abstract
- Lifetimes have been measured in a superdeformed rotational band recently identified in the N = Z nucleus 36Ar. A large low-spin quadrupole deformation (β2=0.46±0.03) is confirmed and a decrease in the collectivity is observed as the high-spin band termination at Iπ=16+ is approached. Detailed comparisons of the experimental B(E2) values with the results of cranked Nilsson-Strutinsky and large-scale (s1/2d3/2)-pf spherical shell model calculations indicate the need for a more refined treatment of transition matrix elements close to termination in the former, and the inclusion of the complete sd-pf model space in the latter description of this highly-collective rotational band.
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| 9. |
- Svensson, C. E., et al.
(författare)
-
Lifetimes of superdeformed rotational states in [Formula Presented]
- 2001
-
Ingår i: Physical Review C - Nuclear Physics. - 0556-2813. ; 63:6, s. 5-5
-
Tidskriftsartikel (refereegranskat)abstract
- Lifetimes have been measured in a superdeformed rotational band recently identified in the [Formula Presented] nucleus [Formula Presented] A large low-spin quadrupole deformation [Formula Presented] is confirmed and a decrease in the collectivity is observed as the high-spin band termination at [Formula Presented] is approached. Detailed comparisons of the experimental [Formula Presented] values with the results of cranked Nilsson-Strutinsky and large-scale [Formula Presented] spherical shell model calculations indicate the need for a more refined treatment of transition matrix elements close to termination in the former, and the inclusion of the complete [Formula Presented] model space in the latter description of this highly-collective rotational band.
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| 10. |
- Svensson, C. E., et al.
(författare)
-
Superdeformation in the N = z Nucleus 36Ar : Experimental, deformed mean field, and spherical shell model descriptions
- 2000
-
Ingår i: Physical Review Letters. - 0031-9007. ; 85:13, s. 2693-2696
-
Tidskriftsartikel (refereegranskat)abstract
- A superdeformed rotational band has been identified in 36Ar, linked to known low-spin states, and observed to its high-spin termination at Iπ = 16+. Cranked Nilsson-Strutinsky and spherical shell model calculations assign the band to a configuration in which four pf-shell orbitals are occupied, leading to a low-spin deformation β2 ≈ 0.45. Two major shells are active for both protons and neutrons, yet the valence space remains small enough to be confronted with the shell model. This band thus provides an ideal case to study the microscopic structure of collective rotational motion.
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| 11. |
- Kelsall, N. S., et al.
(författare)
-
Testing mean-field models near the N=Z line : gamma-ray spectroscopy of the T-z=1/2 nucleus Kr-73
- 2002
-
Ingår i: Physical Review C. Nuclear Physics. - 0556-2813 .- 1089-490X. ; 65:4
-
Tidskriftsartikel (refereegranskat)abstract
- Excited states in the N=Z+1 nucleus Kr-73 have been investigated using the Ca-40(Ar-36,2pn) and Ca-40(Ca-40,alpha2pn) reactions at 145 and 160 MeV, respectively. gamma rays were detected using the Gammasphere array and events were recorded in coincidence with charged-particle and neutron detectors. The three previously observed bands were extended to high spin, and a new unfavored positive-parity band has been observed. The alignment characteristics and decay properties of the bands are all consistent with large-deformation prolate rotation, with no clear evidence for oblate bands or shape coexistence. This is quite different from neighboring Kr-72,Kr-74, indicating a strong shape-stabilizing role for the valence neutron. The experimental results are compared to extended total Routhian surface, cranked Nilsson Strutinsky, and cranked relativistic mean-field calculations. The results suggest that the paired calculations lack some important physics. Neutron-proton correlations may be the missing ingredient. There is also evidence for an unusual band crossing in the negative-parity bands, which may indicate the presence of T=0 pairing correlations. At high spin all the models can reproduce the experimental data.
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| 12. |
- Klamra, Wlodzimierz, et al.
(författare)
-
High-spin multiparticle-hole excitations in Eu-148
- 2001
-
Ingår i: European Physical Journal A. - : Springer Science and Business Media LLC. - 1434-6001 .- 1434-601X. ; 10:1, s. 11-12
-
Tidskriftsartikel (refereegranskat)abstract
- Studies by means of 155 MeV Al-27 bombardment on a Te-130 target revealed in Eu-148 high-spin structures up to spin 31 (n) over tilde, in addition to a cascade extended to the 11088.1 keV excitation. The observed levels are tentatively assigned as complex multiparticle-hole proton and neutron configurations.
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| 13. |
- O'Leary, C. D., et al.
(författare)
-
Evidence for isovector neutron-proton pairing from high-spin states in N = Z 74Rb
- 2003
-
Ingår i: Physical Review C - Nuclear Physics. - 0556-2813. ; 67:2, s. 213011-213015
-
Tidskriftsartikel (refereegranskat)abstract
- High-spin states in the odd-odd N = Z nucleus 3774Rb37 were studied using the 40Ca(40Ca,αnp) reaction. A previously observed odd-spin T = 0 band has been extended to Iπ = (31+) and an even-spin T = 0 band has been observed for the first time to Iπ = (22+); both have a π(g9/2)⊗ν(g9/2) structure. A strongly coupled low-spin T = 0, K = 3 band has been interpreted as being based upon a π[312]3/2 ⊗ ν[312]3/2 configuration. Cranked relativistic Hartree-Bogoliubov calculations, which are corrected for the t = 1 np-pair field by restoring isospin symmetry, reproduce the observed spectrum. These new results provide evidence for the existence of an isovector pair field that contains a neutron-proton component with the proper strength for ensuring isospin conservation.
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| 14. |
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| 15. |
- Ward, D., et al.
(författare)
-
Band Structure of 68Ge
- 2001
-
Ingår i: Physical Review C (Nuclear Physics). - 0556-2813. ; 63:1
-
Tidskriftsartikel (refereegranskat)abstract
- The nucleus Ge-68 has been studied by gamma-ray spectroscopy following its population at high spin in the reaction Ca-40(S-32,4p) Ge-68. The reaction channel was selected with the Microball array and gamma rays were detected with the Gammasphere array. The level scheme is very complex, reflecting the many different, and presumably mixed, excitation modes in this nucleus. Nevertheless, there appear to be some simplifications in the spin range above 18 (h) over bar where we have identified a superdeformed band and several terminating bands. The results are compared with a cranked Nilsson-Strutinsky model without pairing.
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| 16. |
- Asztalos, S. J., et al.
(författare)
-
Isotopic yields of neutron-rich nuclei from deep-inelastic reactions
- 2000
-
Ingår i: Physical Review C. Nuclear Physics. - 0556-2813 .- 1089-490X. ; 6101:1
-
Tidskriftsartikel (refereegranskat)abstract
- We follow up on our earlier work involving a light projectile (Ca-48) to populate high spin states in neutron-rich nuclei with results from experiments involving a heavier projectile (Sm-154) fur the purposes of studying isotopic yields. These yields, which in some cases were mensurable down to a level of 0.1 of the total reaction cross section, are presented from three separate reactions. A trend in the isotopic yields towards N/Z equilibration is observed in one experiment having a large disparity in N/Z ratios between the projectile and target. In the two other reactions, where the N/Z driving force is less pronounced, the yields are instead clustered around the projectile and target nuclei. We present correlated projectilelike and targetlike fragment isotopic yields derived from gamma-gamma coincidences, a technique that enables one to partition the yield of an isotope according to the amount of neutron evaporation. Using this method we find that for the zero-neutron evaporation channel transfer occurs predominantly into the light fragment, consistent with the nature of the deep-inelastic mechanism. We further find that multiple-neutron evaporation contributes substantially to the yields of the isotopes.
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| 17. |
- Ekman, Jörgen, et al.
(författare)
-
The A=51 Mirror Nuclei 51Fe and 51Mn
- 2000
-
Ingår i: European Physical Journal A. Hadrons and Nuclei. - 1434-6001. ; 9, s. 13-17
-
Tidskriftsartikel (refereegranskat)abstract
- Eighteen previously unknown gamma -ray transitions were identified in the T-z = -1/2 nucleus Fe-51 following the fusion-evaporation reaction S-32(Si-28,2 alpha 1n)Fe-51. The lever scheme reaches the fully aligned I-pi = 27/2(-) terminating state of the five holes in the f(7/2) shell. The 17/2(-) state was found to be isomeric, and the lifetime was measured to be 2.87 ns. The mirror symmetry of Fe-51 and Mn-51 is discussed, and the level scheme of Fe-51 is compared to shell-model calculations.
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| 18. |
- Ideguchi, Eiji, et al.
(författare)
-
Superdeformation in 91Tc
- 2000
-
Ingår i: Physics Letters B. - : Elsevier. - 0370-2693 .- 1873-2445. ; 492:3-4, s. 245-253
-
Tidskriftsartikel (refereegranskat)abstract
- A high-spin rotational band with 11 gamma -ray transitions has barn observed in Tc-91. The dynamical moment of inertia as well as the transition quadrupole moment of 8.1(-1.4)(+1.9) eb measured for this band show the characteristics of a superdeformed band. However, the shape is more elongated than in the neighbouring A = 80-90 superdeformed nuclei. Theoretical interpretations of the band within the cranked Strutinsky approach based on two different Woods-Saxon potential parameterisations are presented. Even though an unambiguous configuration assignment proved difficult, both calculations indicate a larger deformation and at least three additional high-N intruder orbitals occupied compared to the lighter SD nuclei. (C) 2000 Elsevier Science B.V. All rights reserved.
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| 19. |
- Kelsall, N.S., et al.
(författare)
-
High-spin studies of N ~ Z nuclei in the mass 70 region
- 2003
-
Ingår i: AIP Conference Proceedings. - 0094-243X .- 1551-7616. ; :656, s. 261-268
-
Konferensbidrag (refereegranskat)abstract
- High-spin states have been studied in 72,73Kr using 40Ca+40Ca and 36Ar+40Ca reactions at 160, 164 and 145 MeV respectively. The properties and configurations of the bands observed are interpreted with the aid of unpaired cranked Nilsson-Strutinsky/ cranked relativistic mean-field calculations and paired total routhian surface and cranked relativistic Hartree-Bogoliubov calculations. In 73Kr the three previously known structures have been extended to a spin of ~30[h-bar] and a fourth structure, which is believed to be the signature partner to the g9/2 band, has been identified. The negative parity bands show evidence for an unusual type of band crossing. It is currently unclear whether this is a consequence of T=0 n-p pairing. At the highest spins the unpaired calculations can reproduce the observed data, suggesting that there is little evidence for pairing correlations in this spin regime. In 72Kr a new band has been identified that has the properties expected from the missing doubly aligned S-band configuration. The properties of this band can be reproduced by the unpaired CNS/ CRMF calculations, again suggesting that the structure has little pairing. However, paired calculations appear to be necessary to reproduce the properties of the previously known structure in this nucleus. The 72Kr results are also compared with projected shell model calculations. ©2003 American Institute of Physics
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| 20. |
- Lagergren, Karin, et al.
(författare)
-
Isospectral superdeformed bands in the N=46 nuclei Mo-88 and Tc-89
- 2004
-
Ingår i: European Physical Journal A. - : Springer Science and Business Media LLC. - 1434-6001 .- 1434-601X. ; 21:3, s. 375-381
-
Tidskriftsartikel (refereegranskat)abstract
- Superdeformed bands in Mo-88 and Tc-89 were populated Using Ca-40-induced fusion-evaporation reactions on Ni-58 at a beam energy of 185 MeV. Gamma-rays emitted in the reactions were detected using the Gammasphere spectrometer, in coincidence with charged particles detected by the Microball array. A new superdeformed band was assigned to the nucleus Mo-88, leading to a revisit of earlier configuration assignments for superdeformed structures in this nucleus. In particular, the theoretical interpretation of a pair of identical (isospectral) superdeformed bands in Mo-88/Tc-89 is discussed. The configurations that are assigned to the four SD bands belonging to Mo-88 have properties that are predicted to be significantly affected by pair correlations.
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| 21. |
- Lagergren, Karin, et al.
(författare)
-
Shape evolution in the superdeformed A approximate to 80-90 mass region
- 2003
-
Ingår i: Physical Review C. Nuclear Physics. - 0556-2813 .- 1089-490X. ; 68:6, s. 643091-643095
-
Tidskriftsartikel (refereegranskat)abstract
- Superdeformed bands in Mo-88, Tc-89, and Tc-91 were populated using a Ca-40 beam with an energy of 185 MeV, impinging on a backed Ni-58 target. gamma rays and charged particles emitted in the reactions were detected using the Gammasphere Ge detector array and the CsI(TI) array Microball. Average transition quadrupole moments Q(t) with significantly improved accuracy compared to earlier work, were deduced for the bands using the residual doppler shift technique. The experimental results were included into a systematic study of the Q(t) values throughout the superdeformed mass 80-90 region. The superdeformed shell gaps are predicted to move towards larger deformations with increasing Z and N in this mass region. This trend is confirmed by the experimental Q(t) values.
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| 22. |
- Lerma, F., et al.
(författare)
-
Superdeformed bands in Sr80-83, Y82-84, Zr-83,Zr-84 : Transition quadrupole moments, moments of inertia, and configuration assignments
- 2003
-
Ingår i: Physical Review C. Nuclear Physics. - 0556-2813 .- 1089-490X. ; 67:4, s. 044310-
-
Tidskriftsartikel (refereegranskat)abstract
- Multiple superdeformed bands in the nuclei Sr80-83, Y82-84, and Zr-83,Zr-84 have been studied in a backed-target experiment, using the Gammasphere and the Microball 4pi detector arrays. For 15 bands in these nuclei, average transition quadrupole moments have been measured with high accuracy, using the Doppler-shift attenuation method. Among these are the newly observed superdeformed bands in Y-83,Y-84, which are isospectral with the bands in Sr-82,Sr-83. These isospectral bands have nearly identical transition quadrupole moments. Two of the bands in Sr-81 interact and cross transitions between them are observed. The present measurements place stringent conditions on configuration assignments for the bands obtained from two types of mean-field calculations, leading to a consistent understanding of the mass similar to80 superdeformed bands. The assignments for different bands arise from one or more nucleons occupying the N-0=5h(11/2) intruder orbital.
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| 23. |
- Reviol, W., et al.
(författare)
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Rotational Bands Near 56Ni
- 2001
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Ingår i: Nuclear Physics, Section A. - 0375-9474. ; 682, s. 28-34
-
Tidskriftsartikel (refereegranskat)abstract
- Rotational bands have been found in Co-57 and Ni-57, using Gammasphere in conjunction with the Microball and 30 neutron detectors. The bands in Co-57, extending the mass 60 region of deformation down to Z = 27, are signature partner sequences. The quadrupole moments and dynamic moments of inertia of the new bands in both nuclei are similar to those of rotational sequences in the neighboring nuclei. The high-spin structure of Co-57 is compared with Skyrme Hartree-Fock calculations.
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| 24. |
- Vanholder, R, et al.
(författare)
-
Uremic toxicity: present state of the art
- 2001
-
Ingår i: The International journal of artificial organs. - : SAGE Publications. - 0391-3988 .- 1724-6040. ; 24:10, s. 695-725
-
Tidskriftsartikel (refereegranskat)abstract
- The uremic syndrome is a complex mixture of organ dysfunctions, which is attributed to the retention of a myriad of compounds that under normal condition are excreted by the healthy kidneys (uremic toxins). In the area of identification and characterization of uremic toxins and in the knowledge of their pathophysiologic importance, major steps forward have been made during recent years. The present article is a review of several of these steps, especially in the area of information about the compounds that could play a role in the development of cardiovascular complications. It is written by those members of the Uremic Toxins Group, which has been created by the European Society for Artificial Organs (ESAO). Each of the 16 authors has written a state of the art in his/her major area of interest.
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| 25. |
- Ekman, Jörgen, et al.
(författare)
-
Gamma-ray Spectroscopy of Core-excited States in 51Mn
- 2004
-
Ingår i: Physical Review C (Nuclear Physics). - 0556-2813. ; 70:1
-
Tidskriftsartikel (refereegranskat)abstract
- The level scheme of Mn-51 has been investigated using the Si-28(S-32 2alpha1p)Mn-51 fusion-evaporation reaction at 125 MeV beam energy. The gamma rays were detected in the Ge-detector array Gammasphere, which was coupled to the 4pi-charged-particle detector Microball and the Neutron Shell for the coincident detection of evaporated particles. Over 100 new gamma-ray transitions connecting some 50 excited states have been identified. Most of the states are built on particle-hole excitations across the shell gaps at particle number N=Z=28. The rich experimental data allows for a 'comprehensive comparison of core-excited states with results from large-scale shell-model calculations, which are known to provide excellent predictions for the lower-lying yrast and near-yrast levels in 1f(7/2) nuclei.
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| 26. |
- Frankenberg, R J, et al.
(författare)
-
Effect of temperature and pressure on the proteolytic specificity of the recombinant 20S proteasome from Methanococcus jannaschii
- 2003
-
Ingår i: Extremophiles. - : Springer Science and Business Media LLC. - 1433-4909 .- 1431-0651. ; 7:5, s. 353-360
-
Tidskriftsartikel (refereegranskat)abstract
- The hydrolytic specificity of the recombinant 20S proteasom from the deep-sea thermophile Methanococcus jannaschii was evaluated toward oxidized insulin B-chain across a range of temperatures (35degrees, 55degrees. 75degrees, and 90degreesC) and hydrostatic pressures (1, 250, 500, and 1,000 atm). Of the four temperatures considered, the same maximum overall hydrolysis rate was observed at both 55degrees and 75degreesC, which are much lower than the T-opt of 116degreesC previously observed for a small amide substrate (Michels and Clark 1997). At 35degreesC the rates of cleavage were highest at the carboxyl side of glutamine and leucine, whereas at the three higher temperatures, the most rapid cleavages occurred after leucine and glutamic acid residues. The distribution of proteolytic fragments and the cleavage sequence also varied between the lowest and higher temperatures. Application of hydrostatic pressure did not increase proteasome activity, as observed previously for the amide substrate (Michels and Clark 1997). but instead significantly reduced the overall conversion of the polypeptide substrate. Overall cleavage patterns observed for the recombinant M. jannaschii proteasome were similar to those reported previously for Thermoplasma acidophilum (Akopian et al. 1997) and human proteasomes (Dick et al. 1991). indicating that proteasome specificity has been conserved despite significant environmental diversity.
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| 27. |
- Guest, PC, et al.
(författare)
-
Proinsulin processing in the diabetic Goto-Kakizaki rat
- 2002
-
Ingår i: The Journal of endocrinology. - : Bioscientifica. - 0022-0795 .- 1479-6805. ; 175:3, s. 637-647
-
Tidskriftsartikel (refereegranskat)abstract
- The biosynthesis and processing of proinsulin was investigated in the diabetic Goto-Kakizaki (GK) rat. Immunofluorescence microscopy comparing GK and Wistar control rat pancreata revealed marked changes in the distribution of alpha-cells and pronounced beta-cell heterogeneity in the expression patterns of insulin, prohormone convertases PC1, PC2, carboxypeptidase E (CPE) and the PC-binding proteins 7B2 and ProSAAS. Western blot analyses of isolated islets revealed little difference in PC1 and CPE expression but PC2 immunoreactivity was markedly lower in the GK islets. The processing of the PC2-dependent substrate chromogranin A was reduced as evidenced by the appearance of intermediates. No differences were seen in the biosynthesis and post-translational modification of PC1, PC2 or CPE following incubation of islets in 16.7 mM glucose, but incubation in 3.3 mM glucose resulted in decreased PC2 biosynthesis in the GK islets. The rates of biosynthesis, processing and secretion of newly synthesized (pro)insulin were comparable. Circulating insulin immunoreactivity in both Wistar and GK rats was predominantly insulin 1 and 2 in the expected ratios with no (pro)insulin evident. Thus, the marked changes in islet morphology and PC2 expression did not impact the rate or extent of proinsulin processing either in vitro or in vivo in this experimental model.
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| 28. |
- Karring, Henrik, et al.
(författare)
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Functional effects of deleting the coiled-coil motif in Escherichia coli elongation factor Ts
- 2003
-
Ingår i: European Journal of Biochemistry. - : Wiley. - 0014-2956. ; 270:21, s. 4294-4305
-
Tidskriftsartikel (refereegranskat)abstract
- Elongation factor Ts (EF-Ts) is the guanine nucleotide-exchange factor for elongation factor Tu (EF-Tu) that is responsible for promoting the binding of aminoacyl-tRNA to the mRNA-programmed ribosome. The structure of the Escherichia coli EF-Tu-EF-Ts complex reveals a protruding antiparallel coiled-coil motif in EF-Ts, which is responsible for the dimerization of EF-Ts in the crystal. In this study, the sequence encoding the coiled-coil motif in EF-Ts was deleted from the genome in Escherichia coli by gene replacement. The growth rate of the resulting mutant strain was 70-95% of that of the wild-type strain, depending on the growth conditions used. The mutant strain sensed amino acid starvation and synthesized the nucleotides guanosine 5'-diphosphate 3'-diphosphate and guanosine 5'-triphosphate 3'-diphosphate at a lower cell density than the wild-type strain. Deletion of the coiled-coil motif only partially reduced the ability of EF-Ts to stimulate the guanine nucleotide exchange in EF-Tu. However, the concentration of guanine nucleotides (GDP and GTP) required to dissociate the mutant EF-Tu-EF-Ts complex was at least two orders of magnitude lower than that for the wild-type complex. The results show that the coiled-coil motif plays a significant role in the ability of EF-Ts to compete with guanine nucleotides for the binding to EF-Tu. The present results also indicate that the deletion alters the competition between EF-Ts and kirromycin for the binding to EF-Tu.
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| 29. |
- Kelsall, NS, et al.
(författare)
-
High-spin structure of N similar or equal to Z nuclei around the A=72 region
- 2004
-
Ingår i: European Physical Journal A. Hadrons and Nuclei. - : Springer Science and Business Media LLC. - 1434-6001. ; 20:1, s. 131-132
-
Tidskriftsartikel (refereegranskat)abstract
- High-spin states have been studied in Kr-72 and Br-72 using the Ca-40 + Ca-40 and Ar-36 + Ca-40 reactions at 164 and 145 MeV, respectively. The properties and configurations of the high-spin bands observed have been interpreted using impaired cranked Nilssou-Strutinsky (CNS), and for Kr-72, paired cranked relativistic Hartree-Bogoliubov (CRHB) calculations. In Kr-72 a new band has been identified that has the properties expected for the doubly aligned S-band configuration. In Br-72 the previously known bands have been extended to higher spin. This has lead to a re-interpretation of the configurations.
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| 30. |
- O'Leary, CD, et al.
(författare)
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Evidence for isovector neutron-proton pairing from high-spin states in N=Z Rb-74
- 2003
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Ingår i: Physical Review C (Nuclear Physics). - 0556-2813. ; 67:2: 021301
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Tidskriftsartikel (refereegranskat)abstract
- High-spin states in the odd-odd N=Z nucleus Rb-74(37)37 were studied using the Ca-40(Ca-40,alphanp) reaction. A previously observed odd-spin T=0 band has been extended to I-pi=(31(+)) and an even-spin T=0 band has been observed for the first time to I-pi=(22(+)); both have a pi(g(9/2))circle timesnu(g(9/2)) structure. A strongly coupled low-spin T=0,K=3 band has been interpreted as being based upon a pi[312]3/2 circle timesnu[312]3/2 configuration. Cranked relativistic Hartree-Bogoliubov calculations, which are corrected for the t=1 np-pair field by restoring isospin symmetry, reproduce the observed spectrum. These new results provide evidence for the existence of an isovector pair field that contains a neutron-proton component with the proper strength for ensuring isospin conservation.
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| 31. |
- Ragnarsson, Ingemar, et al.
(författare)
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Excited Bands in Fixed CNS Configurations
- 2003
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Ingår i: AIP Conference Proceedings. - : AIP. - 1551-7616 .- 0094-243X. ; 656, s. 205-210
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Konferensbidrag (refereegranskat)abstract
- Exact and approximate quantum numbers of the cranked Nilsson-Strutinsky (CNS) formalism are exploited to calculate excited bands in fixed configurations with the energy of the individual bands minimized with respect to deformation for all spin values. The formalism is illustrated to calculate all bands which appear important in the decay out of the superdeformed band in 59Cu
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| 32. |
- Reynolds, R., et al.
(författare)
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Detection of sequence variation in the HVII region of the humanmitochondrial genome in 689 individuals using immobilizedsequence-specific oligonucleotide probes
- 2000
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Ingår i: Journal of Forensic Sciences. - 0022-1198 .- 1556-4029. ; 45:6, s. 1210-1231
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Tidskriftsartikel (refereegranskat)abstract
- We have developed a rapid, immobilized probe-based assay for the detection of sequence variation in the hypervariable segment II (HVII) of the mitochondrial DNA (mtDNA) control region. Using a panel of 17 sequence-specific oligonucleotide (SSO) probes immobilized on nylon membrane strips, we typed 689 individuals from four population groups. The genetic diversity value for each population was calculated from the frequency data, and the frequencies of distinct "mitotypes" in each group were determined. We performed DNA sequence analysis of 129 samples to characterize the sequences associated with "blanks" (absence of probe signals) and weak probe signals. Out of 689 samples, we observed five heteroplasmic samples (excluding the variable C-stretch beginning at position 303) using the immobilized SSO probe panel. The SSO probe strips were used for the analysis of shed hairs and bloodstains from several criminal cases in Sweden, one of which is described here. We conclude that this mtDNA typing system is useful for human identification and significantly decreases casework turnaround time.
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| 33. |
- Sigvardsson, Mikael, et al.
(författare)
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Early B-Cell Factor, E2A, and Pax-5 Cooperate To Activate the Early B Cell-Specific mb-1 Promoter.
- 2002
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Ingår i: Molecular and Cellular Biology. - 0270-7306. ; 22:24, s. 8539-8551
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Tidskriftsartikel (refereegranskat)abstract
- Previous studies have suggested that the early-B-cell-specific mb-1(Ig{alpha}) promoter is regulated by EBF and Pax-5. Here, we used in vivo footprinting assays to detect occupation of binding sites in endogenous mb-1 promoters at various stages of B-cell differentiation. In addition to EBF and Pax-5 binding sites, we detected occupancy of a consensus binding site for E2A proteins (E box) in pre-B cells. EBF and E box sites are crucial for promoter function in transfected pre-B cells, and EBF and E2A proteins synergistically activated the promoter in transfected HeLa cells. Other data suggest that EBF and E box sites are less important for promoter function at later stages of differentiation, whereas binding sites for Pax-5 (and its Ets ternary complex partners) are required for promoter function in all mb-1-expressing cells. Using DNA microarrays, we found that expression of endogenous mb-1 transcripts correlates most closely with EBF expression and negatively with Id1, an inhibitor of E2A protein function, further linking regulation of the mb-1 gene with EBF and E2A. Together, our studies demonstrate the complexity of factors regulating tissue-specific transcription and support the concept that EBF, E2A, and Pax-5 cooperate to activate target genes in early B-cell development.
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| 34. |
- Singh, AK, et al.
(författare)
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Evidence for noncollective oblate structures at high spin in Cs-123
- 2004
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Ingår i: Physical Review C (Nuclear Physics). - 0556-2813. ; 70:3
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Tidskriftsartikel (refereegranskat)abstract
- High-spin states in Cs-123 were populated in the Ni-64(Ni-64,p4n) reaction at a beam energy of 265 MeV. Gamma-ray coincidences were measured using the Gammasphere spectrometer. Two additional bands have been placed in the level scheme and the four previously known bands have been extended to higher spin. At the highest spins, two of the bands show irregular level sequences. These structures of noncollective excitations, which compete with collective rotation, are interpreted as band-terminating states. The results are compared to cranked Nilsson-Strutinsky calculations, and configuration assignments to the bands and to the terminating states are discussed.
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| 35. |
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