| 1. |
- Davidsson, Josef, et al.
(författare)
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Array-based genotype-phenotype correlation in a case of supernumerary ring chromosome 12
- 2008
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Ingår i: Clinical Genetics. - : Wiley. - 0009-9163. ; 73:1, s. 44-49
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Tidskriftsartikel (refereegranskat)abstract
- Supernumerary ring chromosomes (SRC) account for approximately 10% of prenatal marker chromosomes and 60% of these SRCs are associated with an abnormal phenotype of the patient carrying them. SRCs have, with few exceptions, not been characterized at the molecular genetic level. Here, we present the first case of a SRC 12 thoroughly investigated with tiling resolution array-based comparative genomic hybridization (array CGH); multicolor, centromere, subtelomeric and whole chromosome painting fluorescence in situ hybridization. In addition, to be able to correlate phenotypic manifestations with a possible pathogenetic outcome of the SRC 12, we retrospectively compared and reviewed all 14 cases of SRC 12 reported, including our present case. Our analyses revealed that the SRC comprised 25.53-46.40 Mb of chromosome 12, a region known to harbor 47 annotated genes of which nine were of putative pathogenetic relevance. Reviewing the previously described cases of SRC 12, we could not establish any specific recurrent features associated with this type of SRC. This most probably reflects heterogeneity in break-point distribution among the reported cases, resulting in differently sized ring chromosomes and hence varying phenotypic traits of the patients. Detailed genomic evaluation, by array CGH or similar techniques may thus be of importance to predict the clinical course in individual cases.
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| 2. |
- Pozina, Galia, et al.
(författare)
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Phase identification in γ- and κ-alumina coatings by cathodoluminescence
- 2009
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Ingår i: Scripta Materialia. - : Elsevier BV. - 1359-6462 .- 1872-8456. ; 61:4, s. 379-382
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Tidskriftsartikel (refereegranskat)abstract
- Cathodoluminescence (CL) properties were studied for metastable γ- and κ-Al2O3 coatings for cutting tool inserts. The alumina films demonstrate a strong CL, with peak energies depending on the Al2O3 polymorphs. The phase transformation caused by heat treatment is shown to correlate with strong CL modification in the annealed coatings. Spatially resolved CL is found to be a powerful nondestructive technique for identification of different alumina phases on the microscopic level. The corresponding optical properties of γ- and κ-Al2O3 are discussed.
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| 3. |
- Trinh, David, 1981-, et al.
(författare)
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Coated Insert
- 2006
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Patent (populärvet., debatt m.m.)
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| 4. |
- Trinh, David Huy, 1981-, et al.
(författare)
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Nanocomposite Al2O3-ZrO2 thin films grown by reactive dual radio-frequency magnetron sputtering
- 2008
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Ingår i: Thin Solid Films. - : Elsevier. - 0040-6090 .- 1879-2731. ; 516:15, s. 4977-4982
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Tidskriftsartikel (refereegranskat)abstract
- Crystalline alumina–zirconia nanocomposites have been synthesized at 450 °C and 750 °C with reactive magnetron sputtering using radio-frequency power supplies. The composition of the films ranged from pure alumina to pure zirconia as measured by ion beam techniques. Microstructural characterization showed the presence of monoclinic zirconia in the pure zirconia films and γ-alumina in the pure alumina films while the nanocomposites contained either an amorphous compound, γ-alumina, cubic zirconia or a mixture of these. The grain size was 5 nm for the nanocomposite compared to larger grains in the pure oxide films. Electron energy loss spectroscopy showed a clear progression from the pure alumina to the pure zirconia.
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| 5. |
- Trinh, David Huy, 1981-
(författare)
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Nanocrystalline Alumina-Zirconia Thin Films Grown by Magnetron Sputtering
- 2008
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Alumina-zirconia thin films have been deposited using dual magnetron sputtering. Film growth was performed at relatively low-to-medium temperatures, ranging from ~300°C to 810 °C. Different substrates were applied, including silicon (100), and industrially relevant materials, such as WC-Co hardmetal. Both radio-frequency sputtering and direct-current magnetron sputtering were utilised to achieve a range of film compositions. The influence of sputtering target was investigated; both ceramics and metals were used as sputtering sources. Microstructural characterisation was performed with a range of electron microscopy and x-ray diffraction techniques which show that the pure zirconia was deposited in the monoclinic phase. Reduced mobility of depositing species, as in the case of direct-current sputtering, yielded preferred crystallographic orientation in the {100} directions. The initial nucleation layer consisted of the metastable tetragonal zirconia phase. This phase could be grown over film thicknesses ~1 μm through the addition of ~3 at.% Al under similar low mobility conditions. For cases of higher mobility, as obtained through radio-frequency sputtering, the metastable cubic zirconia phase formed in the film bulk for alumina-zirconia nanocomposites. A combination of two mechanisms is suggested for the stabilisation of metastable zirconia phases: oxygen-deficiency and aluminium segregations with resultant restraint on the zirconia lattice. The sputter deposition process was investigated through energy resolved mass spectrometry in the case of radio-frequency sputtering; the sputter deposition flux contained a mixture of metallic ions, metaloxygen clusters, and oxygen ions. The presence of metal-oxygen clusters was found to be important in oxygen-stoichiometry and thus the phase selection of the resultant film. The energy distributions were similar when comparing sputtering from ceramic and metallic targets. A mass-balance model has also been developed for the transport phenomena and reactions of particles in reactive sputtering of two targets in a two-gas scenario for the alumina-zirconia system. Addition of nitrogen to the working gas was found to eliminate the hysteresis in the target poisoning for oxygen reactive sputtering. The higher reactivity of oxygen contributed to a higher oxygen content in resultant films compared to the oxygen content in the oxy-nitride working gas. The model was thus shown to be successful for tuning depositions in the alumina-zirconia oxy-nitride system.
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| 6. |
- Trinh, David Huy, et al.
(författare)
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Radio frequency dual magnetron sputtering deposition and characterization of nanocomposite Al2O3-ZrO2 thin films
- 2006
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Ingår i: Journal of Vacuum Science & Technology. A. Vacuum, Surfaces, and Films. - : American Vacuum Society. - 0734-2101 .- 1520-8559. ; 24:2, s. 309-316
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Tidskriftsartikel (refereegranskat)abstract
- Radio frequency magnetron sputtering from oxide targets has been used to synthesize crystalline alumina–zirconia nanocomposites at a relatively low temperature of 450 °C. Films of different compositions have been deposited ranging from pure zirconia to pure alumina, the compositions being measured with Rutherford backscattering and elastic recoil detection analysis. X-ray diffraction studies show the presence of the monoclinic zirconia phase in pure zirconia films. Addition of alumina into the film results in the growth of the cubic zirconia phase and amorphous alumina. No crystalline alumina was detected in either the composite or the pure alumina film. The microstructure of the films as studied by high resolution electron microscopy and scanning transmission electron microscopy shows a columnar growth mode in both the pure zirconia and nanocomposite films, but reveals differences in the intracolumnar structure. For the nanocomposite small equiaxed grains, ~5 nm in size, are found at the base of the columns at the interface with the substrate. An amorphous tissue of alumina was present between the small crystallites in the case of the nanocomposite.
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| 7. |
- Trinh, David, et al.
(författare)
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Phase transformation in kappa- and gamma-Al2O3 coatings on cutting tool inserts
- 2009
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Ingår i: Surface & Coatings Technology. - : Elsevier BV. - 0257-8972 .- 1879-3347. ; 203:12, s. 1682-1688
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Tidskriftsartikel (refereegranskat)abstract
- The phase transformation in as-deposited, metastable kappa- and gamma-alumina coatings on cutting inserts has been studied by a combination of X-ray diffraction, scanning electron microscopy and cathodoluminescence. In the case of kappa-alumina, mechanical forces during metal cutting lower the transformation temperature of the metastable phases to the thermodynamically stable alpha-alumina phase from 1050 degrees C to 930 degrees C. This is the reason why that coating has a similar performance during metal cutting when compared to the stable ce-alumina phase. The transformation temperature in gamma-alumina coated cutting tools is found to be as low as 950-975 degrees C. Cathodoluminescence (CL) has been demonstrated as a possible method for differentiation between the various alumina phases. Specifically, both kappa- and gamma-alumina films have revealed a strong room temperature CL with different peak energies depending on the phase. CL of the metastable alumina coatings annealed at the transformation temperatures corresponds to the stable alpha-alumina phase.
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| 8. |
- Vestin, Fredrik, et al.
(författare)
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Rotational coherent anti-Stokes Raman spectroscopy (CARS) applied to thermometry in high-pressure hydrocarbon flames
- 2008
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Ingår i: Combustion and Flame. - : Elsevier BV. - 0010-2180. ; 154:1-2, s. 143-152
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Tidskriftsartikel (refereegranskat)abstract
- Dual-broadband rotational coherent anti-Stokes Raman spectroscopy (DB-RCARS) has been investigated for thermometry under high-pressure and high-temperature conditions, in the product gas of fuel-lean hydrocarbon flames up to 1 MPa. Initial calibration measurements made in nitrogen, oxygen, and air, at pressures up to 1.55 MPa and temperatures up to 1800 K, showed good agreement between experimental and theoretical spectra. In the high-pressure flames, high-quality single-shot spectra were recorded in which nitrogen lines dominated, and peaks from CO2 and O-2 were also visible. A spectral model including the species N-2, CO2, and O-2, as well as the best available Raman linewidth models for flame thermometry, were used to evaluate the experimental spectra. Experimental problems as well as considerations related to the spectral evaluation are discussed. This work demonstrates the significant potential of DB-RCARS thermometry for applications in high-pressure and high-temperature environments.
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