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Träfflista för sökning "WFRF:(Dalboge H) srt2:(1989)"

Search: WFRF:(Dalboge H) > (1989)

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  • Dalboge, H, et al. (author)
  • High-level expression of active human cystatin C in Escherichia coli
  • 1989
  • In: Gene. - 1879-0038. ; 79:2, s. 325-332
  • Journal article (peer-reviewed)abstract
    • Expression of the human cysteine proteinase inhibitor, cystatin C (CysC) in the cytoplasm of Escherichia coli was studied using a cDNA fragment encoding the cysteine proteinase inhibitor controlled by the phage λ pImage /cI857 system. The yield of CysC was low, probably due to proteolytic degradation. By fusing the cysC cDNA to a DNA fragment encoding the signal peptide of the E. coli outer membrane protein A, it was possible to produce a substantial amount of CysC in the periplasm. The processing of the signal peptide was shown to be quantitative and to result in CysC with the correct N-terminal amino acid. Yields higher than 1000 μg CysC/ml can be obtained by initiating the product formation at a moderate temperature (40 °C) late in an optimized fermentation process. A method that gives selective extraction of the periplasmic proteins and at the same time stabilizes CysC has been used.
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  • Result 1-2 of 2
Type of publication
conference paper (1)
journal article (1)
Type of content
peer-reviewed (2)
Author/Editor
Abrahamson, Magnus (2)
Olafsson, I (2)
Grubb, Anders (2)
Dalboge, H (2)
Tottrup, H (2)
Carlsen, S (2)
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Jensen, EB (1)
Katunuma, Nobuhiko (1)
Kominami, Eiki (1)
Bech Jensen, E (1)
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University
Lund University (2)
Language
English (2)
Research subject (UKÄ/SCB)
Medical and Health Sciences (2)
Year
1989 (2)Undo refine

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