| 1. |
- Wang, Zhaohui, et al.
(författare)
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Clinical significance and pathogenic role of anti-cardiac myosin autoantibody in dilated cardiomyopathy.
- 2003
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Ingår i: Chinese medical journal. - 0366-6999. ; 116:4, s. 499-502
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: In order to explore the possible roles played by the autoimmune mechanism in the progression of myocarditis into dilated cardiomyopathy (DCM) using an animal model, we investigated whether autoimmune myocarditis might develop into DCM. METHODS: Experimental Balb/C mice (n = 20) were immunized with cardiac myosin with Freund's complete adjuvant at days 0, 7 and 30. The control Balb/C mice (n = 10) were immunized with Freund's complete adjuvant in the same mannere. Serum and myocardium samples were collected after the first immunization at days 15, 21 and 120. The anti-myosin antibody was examined by enzyme-linked immunosorbent assay and immunoblotting. RESULTS: Pathological findings demonstrated that there was myocardial necrosis or inflammatory infiltration during acute stages and fibrosis mainly in the late phase of experimental group, but the myocardial lesions were not found in the control group. Autoimmunity could induce myocarditis and DCM in the absence of viral infection. High titer anti-myosin IgG antibodies were found in the experimental group, but not in the control group. Furthermore, the anti-myosin heavy chain (200 KD) antibody was positive in 21 of 48 patients with DCM and viral myocarditis, but only 4 of 20 patients with coronary heart disease, including 1 case and 3 cases that reacted with heavy and light chains (27.5 KD), respectively. The antibodies were not detected in healthy donors. CONCLUSION: Cardiac myosin might be an autoantigen that provokes autoimmunity and leads to the transformation of myocarditis into DCM. Detection of anti-myosin heavy chain antibody might contribute to diagnosis for DCM and viral myocarditis.
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| 2. |
- Youn, Byeng Dong, et al.
(författare)
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Techniques for estimating uncertainty propagation in probabilistic design of multilevel systems
- 2004
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Ingår i: A collection of technical papers. - Reston, Va. : American Institute of Aeronautics and Astronautics, AIAA. - 1563477165 ; , s. 1893-1902
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Konferensbidrag (refereegranskat)abstract
- In probabilistic design of multilevel systems, the challenge is to estimate uncertainty propagation since outputs of subsystems at lower levels constitute inputs of subsystems at higher levels. Three uncertainty propagation estimation techniques are compared in this paper in terms of numerical efficiency and accuracy: root sum square (linearization), distribution-based moment approximation, and Taguchi-based integration. When applied to simulation-based, multilevel system design optimization under uncertainty, it is investigated which type of applications each method is best suitable for. The probabilistic formulation of the analytical target cascading methodology is used to solve the multilevel problem. A hierarchical bi-level engine design problem is employed to investigate unique features of the presented techniques for uncertainty propagation. This study aims at helping potential users to identify appropriate techniques for their applications. Copyright © 2004 by the American Institute of Aeronautics and Astronautics, Inc. All rights reserved.
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| 3. |
- Yuan, Hai-Tao, et al.
(författare)
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Prevention of myosin-induced autoimmune myocarditis in mice by anti-L3T4 monoclonal antibody.
- 2003
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Ingår i: Canadian journal of physiology and pharmacology. - : Canadian Science Publishing. - 0008-4212 .- 1205-7541. ; 81:2, s. 84-8
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Tidskriftsartikel (refereegranskat)abstract
- This study was aimed at studying the effect of the induction of immune tolerance to swine cardiac myosin from anti-L3T4 monoclonal antibody injection and whether the immune tolerance could protect mice with myosin-induced myocarditis from myocardial injury. Twenty-four Balb/c mice were divided into two groups at random. All of the mice were immunized with swine cardiac myosin on the 1st day, 14th, 28th, 42nd, and 52nd day. Immune tolerance was induced by triplicate injections of 400 microg anti-L3T4 McAb on the 0 day (intravenous), 1st day, and 2nd day (intraperitoneal) in McAb-treated group. In the saline-treated group, saline of the same volume as anti-L3T4 monoclonal antibody was used as a control. The sera and hearts biopsies of all mice were collected on the 58th day. The anti-cardiac myosin antibody was examined with ELISA, and pathological changes of heart were observed by light microscope. It was shown that mice immunized with swine cardiac myosin could produce anti-myosin antibody and the anti-cardiac myosin antibody was positive in most of the saline-treated group but negative in the McAb-treated group. Morphologically, myocardial degeneration, necrosis, and infiltration of inflammatory cells were found in the saline-treated group but not in the McAb-treated group. In conclusion, this study indicated that the immune tolerance to cardiac myosin was induced by the anti-L3T4 monoclonal antibody, and accordingly myocardial injury could be prevented by induction of immune tolerance.
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