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- Asplund, Anna, et al.
(författare)
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Expression profiling of microdissected cell populations selected from basal cells in normal epidermis and basal cell carcinoma
- 2008
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Ingår i: British Journal of Dermatology. - : Oxford University Press (OUP). - 0007-0963 .- 1365-2133. ; 158:3, s. 527-538
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Tidskriftsartikel (refereegranskat)abstract
- Background Basal cell carcinomas (BCCs) are prevalent tumours with uniform histology that develop without any known precursor lesion. Alterations in the sonic hedgehog-patched1 signalling pathway are accepted as necessary events for tumorigenesis, and mutations in the patched1 gene are frequently present in tumours. Objectives To analyse transcript profiles in BCC. Methods We used laser-assisted microdissection to isolate and collect cell populations defined under the microscope. Peripheral cells from nests of BCC were selected to represent tumour cells, and normal keratinocytes from epidermis basal layer were used as control. Extracted RNA was amplified and hybridized on to a cDNA microarray. Results Our results show that BCC cells express a transcript signature that is significantly different from that of normal keratinocytes, and over 350 genes with various functions were identified as differentially expressed. The compiled data suggest an upregulation of the Wnt signalling pathway as a major event in BCC cells. Furthermore, tumour cells appear to have an increased sensitivity to oxygen radicals and dysregulated genes involved in antigen presentation. Results were validated at both the transcriptional level using real-time polymerase chain reaction and at the protein level using immunohistochemistry. Conclusions We show that microdissection in combination with robust strategies for RNA extraction, amplification and cDNA microarray analysis allow for reliable transcript profiling and that antibody-based proteomics provides an advantageous strategy for the analysis of corresponding differentially expressed proteins. We found that expression patterns were significantly altered in BCC cells compared with basal keratinocytes and that the Wnt signalling pathway was upregulated in tumour cells.
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- Bergström, L., et al.
(författare)
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Signals of WIMP annihilation into electrons at the galactic center
- 2005
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Ingår i: Proceedings of the 29th International Cosmic Ray Conference, Vol 4: OG 2.1, 2.2 & 2.3. - : Tata Institute of Fundamental Research. ; , s. 57-60
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Konferensbidrag (refereegranskat)abstract
- Photons from the annihilation of dark matter in the center of our Galaxy are expected to provide a promising way to find out the nature and distribution of the dark matter itself. These photons can be either produced directly and/or through successive decays of annihilation products, or radiated from electrons and positrons. This ends up in a multi-wavelength production of photons whose expected intensity can be compared to observational data. Assuming that the Lightest Supersymmetric Particle makes the dark matter, we derive the expected photon signal from a given dark matter model and compare it with present available data.
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- Laure, Erwin, et al.
(författare)
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Programming the Grid using gLite
- 2006
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Ingår i: Computational Methods in Science and Technology. - 1505-0602. ; 12:1, s. 33-45
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The past few years have seen the creation of the first production level Grid infrastructures that offer their users a dependableservice at an unprecedented scale. Depending on the flavor of middleware services these infrastructures deploy (for instance Condor,gLite, Globus, UNICORE, to name only a few) different interfaces to program the Grid infrastructures are provided. Despite ongoingefforts to standardize Grid service interfaces, there are still significant differences in how applications can interface to a Grid infrastructure.In this paper we describe the middleware (gLite) and services deployed on the EGEE Grid infrastructure and explain how applicationscan interface to them.
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- Laure, Erwin, et al.
(författare)
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Programming the grid with glite
- 2006
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Ingår i: Computational Methods in Science and Technology. - 1505-0602. ; 12:1, s. 33-45
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The past few years have seen the creation of the first production level Grid infrastructures that offer their users a dependable service at an unprecedented scale. Depending on the flavor of middleware services these infrastructures deploy (for instance Condor, gLite, Globus, UNICORE, to name only a few) different interfaces to program the Grid infrastructures are provided. Despite ongoing efforts to standardize Grid service interfaces, there are still significant differences in how applications can interface to a Grid infrastructure. In this paper we describe the middleware (gLite) and services deployed on the EGEE Grid infrastructure and explain how applications can interface to them.
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- Lundstedt, G., et al.
(författare)
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Eating disorder traits in obese children and adolescents
- 2006
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Ingår i: Eating and Weight Disorders. - 1124-4909 .- 1590-1262. ; 11:1, s. 45-50
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the present study was to investigate the prevalence of eating disorder (ED) traits among obese children and adolescents. The Swedish version of the Eating Disorder Inventory for Children, consisting of 11 subscales, was administered to 150 obese patients during an extensive investigation of causes and risk factors in obesity at the Karolinska University Hospital at Huddinge. Patients aged 17-18 years (N=24) had a mean body mass index (BMI) of 40.7, SD 5.31, and patients aged 8-16 (N=126) had a mean body mass index standard deviation score (BMI SDS) of 6.18, SD 1.69. These patients were compared with 201 girls with a diagnosed ED from the COEAT project and with a control group of schoolchildren. The comparison between obese girls and boys showed that adolescent obese girls scored higher than obese boys on Drive for Thinness, Bulimia and Body Dissatisfaction. They also scored higher on Ineffectiveness, Interoceptive Awareness and Impulse Regulation. Obese girls were close to the girls with an ED on six of the subscales. Obese boys had a lower score of Asceticism than boys in the control group. The conclusion is that psychological traits associated with disordered eating appear among obese patients, particularly among the girls. However, these patients rarely satisfy any diagnostic criteria for ED during childhood or adolescence. Since obesity treatment currently assumes rational behavior, i.e. no EDs, it is important to discover ED traits at an early age in order to adapt treatment accordingly.
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- Ricordi, C, et al.
(författare)
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Toward a renewable source of pancreatic beta-cells
- 2008
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Ingår i: Nature biotechnology. - : Springer Science and Business Media LLC. - 1546-1696 .- 1087-0156. ; 26:4, s. 397-398
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
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- Segersten, M. Ulrika, et al.
(författare)
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A novel strategy based on histological protein profiling in-silico for identifying potential biomarkers in urinary bladder cancer
- 2009
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Ingår i: BJU International. - 1464-4096 .- 1464-410X. ; 104:11, s. 1780-1785
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To screen a publicly available immunohistochemistry (IHC) based web-atlas, to identify key proteins in bladder cancer that might serve as potential biomarkers. MATERIALS AND METHODS: The first version of the Human Protein Atlas (HPA 1.0), with 660 proteins, was visually examined to identify proteins with a variable staining pattern among the 12 tissue samples representing bladder cancer. None or limited previous characterization in bladder cancer, as well as a supportive Western blot, were also required. The selected proteins were then evaluated in an independent set of patient samples (106 tumour samples of differing stage and grade) represented in a tissue microarray (TMAi). The IHC expression of the identified proteins in the TMAi was scored and related to tumour stage and grade. RESULTS: The expression profiles of the 13 proteins selected from the web-atlas were confirmed in the TMAi. Expression patterns for seven proteins were significantly altered (P < 0.05) with higher stage and/or grade. Three of those (CN130, DSG3, PHF6) lack characterization in bladder cancer, whereas the remaining four proteins have previously been suggested as key proteins/potential biomarkers in cancer, some of them also in bladder cancer. CONCLUSION: New candidate proteins for urinary bladder cancer were identified through screening of the publicly available HPA 1.0. Although further evaluation is necessary, this strategy is promising in the search for new biomarkers, with potential to improve the management of patients with this disease.
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- Wirén, K, et al.
(författare)
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Treatment with a barrier-strengthening moisturizing cream delays relapse of atopic dermatitis : a prospective and randomized controlled clinical trial
- 2009
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Ingår i: Journal of the European Academy of Dermatology and Venereology. - : Wiley. - 0926-9959 .- 1468-3083. ; 23:11, s. 1267-1272
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Tidskriftsartikel (refereegranskat)abstract
- Background: Standard treatment of atopic dermatitis (AD) is based on topical glucocorticosteroids or calcineurin inhibitors to treat flares combined with moisturizer treatment to alleviate dry skin symptoms. Patients with AD have an abnormal skin barrier function, and strategies for reducing the risks for eczema would be to repair the barrier or prevent barrier dysfunction. Objectives: The objective of this study was to explore the time to relapse of eczema during a 26-week maintenance treatment with a urea containing moisturizer compared to no treatment (neither medical nor non-medicated preparations) after successful clearing of atopic lesions. The moisturizer has previously been shown to improve skin barrier function. Methods: Patients applied betamethasone valerate (0.1%) on eczematous lesions during a 3-week period. Those with cleared eczema entered a 26-week maintenance phase, applying the moisturizer or left the previously affected area untreated. Upon eczema relapse, patients were instructed to contact the clinic and to have the relapse confirmed by the investigator. Results: Fifty-five patients entered the study and 44 patients were included in the maintenance phase (22 using moisturizer twice daily and 22 using no treatment). Median time to relapse for patients treated with moisturizer was > 180 days (duration of the study) compared with 30 days for the no-treatment group. Sixty-eight per cent of the patients treated with the moisturizer and 32% of the untreated patients remained free from eczema during the observation period. Conclusions: Maintenance treatment with a barrier-improving urea moisturizer on previous eczematous areas reduced the risk of relapse to approximately one third of that of no treatment.
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