| 1. |
|
|
| 2. |
- Edwards, Y., et al.
(författare)
-
Quality-assured solutions for green roof gardens on concrete decks with zero tolerance for leaks
- 2014
-
Ingår i: Wit Transactions on Ecology and The Environment. - : WITPress. - 1746-448X .- 1743-3541. ; 191, s. 635-645
-
Tidskriftsartikel (refereegranskat)abstract
- Eco neighborhoods with green infrastructure solutions are increasingly being prescribed today in Swedish cities for reducing and attenuating storm water runoff, increasing biodiversity, having a temperature moderating effect and for energy saving. Thus, contractors are simply required to build with green gardens on concrete decks, such as green roofs, green terraces, green courtyards and green complete neighborhoods. A lot of knowledge and experience is in fact lacking today, and consequences may therefore be devastating. If green system solutions are to be seen as an obvious choice in future settlements, and not as a problem, there must be clear guidelines and specifications that ensure a sustainable outcome. This is missing today. This paper reports a project aiming at bringing together researchers, government and industry to the collaborative development of new and attractive solutions for green roof gardens with consideration to the environment and high requirements for durability, materials, construction and energy efficiency. These solutions must also be adaptable to similar types of facilities, specific needs and environments. One such area concerns public land such as parks, streets and squares on concrete decks. Certification and tailored guidelines for different types of systems are being developed. The initiating part of this project clearly indicates that there is a need for better understanding, more research and long term monitoring/follow up of green roofs. Furthermore, a holistic approach is introduced to ensure that one good green roof function will not have severe negative effects on other functions.
|
|
| 3. |
- Emilsson, Gustav, 1989, et al.
(författare)
-
Identifying bacteria using DNA binding maps
- 2013
-
Ingår i: 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Freiburg; Germany; 27 October 2013 through 31 October 2013. - 9781632666246 ; 1, s. 473-475
-
Konferensbidrag (refereegranskat)abstract
- We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.
|
|
| 4. |
- Emilsson, Tobias
(författare)
-
Green roof systems: A study of public attitudes and preferences in southern Spain
- 2013
-
Ingår i: Journal of Environmental Management. - : Elsevier BV. - 0301-4797 .- 1095-8630. ; 128, s. 106-115
-
Tidskriftsartikel (refereegranskat)abstract
- This study investigates people's preconceptions of green roofs and their visual preference for different green roof design alternatives in relation to behavioral, social and demographical variables. The investigation was performed as a visual preference study using digital images created to represent eight different alternatives: gravel roof, extensive green roof with Sedums not in flower, extensive green roof with sedums in bloom, semi-intensive green roof with sedums and ornamental grasses, semi-intensive green roof with shrubs, intensive green roof planted with a lawn, intensive green roof with succulent and trees and intensive green roof with shrubs and trees. Using a Likert-type scale, 450 respondents were asked to indicate their preference for each digital image. Results indicated that respondents' sociodemographic characteristics and childhood environmental background influenced their preferences toward different green roof types. Results also showed that green roofs with a more careful design, greater variety of vegetation structure, and more variety of colors were preferred over alternatives. (C) 2013 Elsevier Ltd. All rights reserved.
|
|
| 5. |
|
|
| 6. |
|
|
| 7. |
|
|
| 8. |
- Gao, Tian, et al.
(författare)
-
The role of forest stand structure as biodiversity indicator
- 2014
-
Ingår i: Forest Ecology and Management. - : Elsevier BV. - 0378-1127 .- 1872-7042. ; 330, s. 82-93
-
Tidskriftsartikel (refereegranskat)abstract
- Biodiversity conservation is a key objective for sustainable forest management, but the multi-dimensional and multi-scale character of biodiversity renders full assessment difficult at large scale. Therefore, indicators are often used to monitor biodiversity. Important cost-benefit synergies can be achieved if indicators are derived from existing data. In this study, a model for classifying forest stand structures was developed and tested as an indicator of overall plant species diversity at stand level. The model combines four stand structure parameters: canopy coverage, age of canopy trees, tree species composition and canopy stratification. Using data from the National Inventory of Landscapes in Sweden and General Linear Mixed Model, plant species diversity (Shannon diversity index, SHDI) and composition (Sorensen-Dice index, SDI) were tested between 26 different stand structure types and nine soil classes. The results showed that mature stands with a stratified canopy had the highest plant species diversity across the soil classes, particularly if they comprised mixed coniferous and broadleaved species with a semi-open canopy. In contrast, young (<30 years) single-layered stands had consistently low species diversity. Of the four stand structure parameters in the model, age of canopy trees was most influential for SHDI value, followed by canopy stratification, tree species composition and canopy coverage. According to the SDI values, different stand structure types represented different species composition regardless of soil class and species diversity (SHDI value). However, most SDI values were higher than 0.5, indicating that fewer than 50% of the species changed between stand structure types. The stand parameters included in the model can probably be extracted from national forest inventories in many countries and understood without specialist taxonomic knowledge, making the model applicable in practice to support forest management decision-making on enhancing forest biodiversity at stand level. (C) 2014 Elsevier B.V. All rights reserved.
|
|
| 9. |
- Nilsson, Adam, et al.
(författare)
-
Competitive binding-based optical DNA mapping for fast identification of bacteria - multi-ligand transfer matrix theory and experimental applications on Escherichia coli.
- 2014
-
Ingår i: Nucleic Acids Research. - : Oxford University Press (OUP). - 1362-4962 .- 0305-1048. ; 42:15, s. 118-118
-
Tidskriftsartikel (refereegranskat)abstract
- We demonstrate a single DNA molecule optical mapping assay able to resolve a specific Escherichia coli strain from other strains. The assay is based on competitive binding of the fluorescent dye YOYO-1 and the AT-specific antibiotic netropsin. The optical map is visualized by stretching the DNA molecules in nanofluidic channels. We optimize the experimental conditions to obtain reproducible barcodes containing as much information as possible. We implement a multi-ligand transfer matrix method for calculating theoretical barcodes from known DNA sequences. Our method extends previous theoretical approaches for competitive binding of two types of ligands to many types of ligands and introduces a recursive approach that allows long barcodes to be calculated with standard computer floating point formats. The identification of a specific E. coli strain (CCUG 10979) is based on mapping of 50-160 kilobasepair experimental DNA fragments onto the theoretical genome using the developed theory. Our identification protocol introduces two theoretical constructs: a P-value for a best experiment-theory match and an information score threshold. The developed methods provide a novel optical mapping toolbox for identification of bacterial species and strains. The protocol does not require cultivation of bacteria or DNA amplification, which allows for ultra-fast identification of bacterial pathogens.
|
|
