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The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems

Emmelkamp, J. (author)
Wolbers, F. (author)
Andersson, Helene (author)
KTH,Bioteknologi
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DaCosta, R. S. (author)
Wilson, B. C. (author)
Vermes, I. (author)
van den Berg, A. (author)
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 (creator_code:org_t)
Wiley, 2004
2004
English.
In: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 25:21-22, s. 3740-3745
  • Journal article (peer-reviewed)
Abstract Subject headings
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  • A novel method for studying unlabeled living mammalian cells based on their autofluorescence (AF) signal in a prototype microfluidic device is presented. When combined, cellular AF detection and microfluidic devices have the potential to facilitate high-throughput analysis of different cell populations. To demonstrate this, unlabeled cultured cells in microfluidic devices were excited with a 488 nm excitation light and the AF emission (> 505 nm) was detected using a confocal fluorescence microscope (CFM). For example, a simple microfluidic three-port glass microstructure was used together with conventional electroosmotic flow (EOF) to switch the direction of the fluid flow. As a means to test the potential of AF-based cell sorting in this microfluidic device, granulocytes were successfully differentiated from human red blood cells (RBCs) based on differences in AF This study demonstrated the use of a simple microfabricated device to perform high-throughput live cell detection and differentiation without the need for cell-specific fluorescent labeling dyes and thereby reducing the sample preparation time. Hence, the combined use of microfluidic devices and cell AF may have many applications in single-cell analysis.

Subject headings

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

Keyword

autofluorescence
microfluidic
miniaturization
red blood cell
single-cell analysis
spectroscopy
transport

Publication and Content Type

ref (subject category)
art (subject category)

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