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Träfflista för sökning "WFRF:(Fernandez A.) srt2:(1985-1989)"

Sökning: WFRF:(Fernandez A.) > (1985-1989)

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1.
  • Fernandez-Luna, J L, et al. (författare)
  • A sensitive and rapid enzyme-linked immunosorbent assay using monoclonal antibodies for simultaneous quantitation of free and IgA-complexed protein HC
  • 1985
  • Ingår i: Journal of Immunological Methods. - : Elsevier BV. - 0022-1759. ; 82:1, s. 101-110
  • Tidskriftsartikel (refereegranskat)abstract
    • A sensitive and rapid enzyme-linked immunosorbent assay for simultaneous quantitation of free and IgA-complexed human protein HC was developed with monoclonal murine antibodies. The total amount of protein HC (free plus IgA-complexed) was measured by a competitive procedure while the protein HC-IgA complex was quantitated by a sandwich enzyme immunoassay. The amount of free protein HC was then obtained as the difference between the 2 measured values. The sensitivity of the procedure was 70 micrograms/1 for the total amount of protein HC and 80 micrograms/1 for the protein HC-IgA complex. At ordinary human serum levels of free protein HC and protein HC-IgA complexes the coefficient of variation for the procedure was about 5%. One worker could determine the concentrations of free and IgA-complexed protein HC in up to 400 serum samples in a working day.
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2.
  • Méndez, Enrique, et al. (författare)
  • Human protein HC and its IgA complex are inhibitors of neutrophil chemotaxis
  • 1986
  • Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424. ; 83:5, s. 1472-1475
  • Tidskriftsartikel (refereegranskat)abstract
    • Protein HC, a heterogeneously charged low molecular weight glycoprotein, and its IgA complex were isolated from human plasma and urine. Plasma from individuals with monoclonal IgA populations was used as starting material for the isolation of the protein HC-IgA complex to obtain homogeneous complex populations. Neither low molecular weight protein HC nor its IgA complex in the concentrations 30 and 600 mg/liter influenced the random migration of normal human neutrophils. The chemotactic response of neutrophils to endotoxin-activated serum was, however, attenuated in a dose-dependent way by both low molecular weight protein HC and protein HC-IgA complex. Concentrations of protein HC and its IgA complex producing significant inhibition of the chemotactic response were found to occur in plasma from healthy and diseased individuals as well as in synovial fluid from patients with rheumatoid arthritis. These results suggest that protein HC and its IgA complex play physiological roles in the regulation of the inflammatory response.
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  • Resultat 1-2 av 2
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tidskriftsartikel (2)
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refereegranskat (2)
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Grubb, A (2)
Méndez, E (1)
Méndez, Enrique (1)
Fernandez-Luna, J L (1)
Moneo, Ignacio (1)
Fernández-Luna, Jose ... (1)
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Lunds universitet (2)
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Engelska (2)
Forskningsämne (UKÄ/SCB)
Medicin och hälsovetenskap (2)

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