| 1. |
- Ahuja-Jensen, Poonam, et al.
(författare)
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Low glutathione peroxidase in rdl mouse retina increases oxidative stress and proteases
- 2007
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Ingår i: NeuroReport. - 1473-558X. ; 18:8, s. 797-801
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Tidskriftsartikel (refereegranskat)abstract
- Malondialdehyde, reduced glutathione, glutathione peroxidase, glutathione reductase and cysteine protease cathepsins at postnatal (PN) days 2, 7, 14, 21 and 28 in controls (wt) and the retinal degeneration 1 (rd1) mouse model for retinitis pigmentosa retinas were measured to determine oxidative stress. In PN28 wt and PN2 rd1 retinas, elevated malondialdehyde and low glutathione peroxidase activity indicate higher oxidative load, despite higher reduced glutathione in PN2 rd1 retinas. This is due to physiological exposure to light and retinal vascular/neural restructuring, respectively. Compared with wt retinas, relatively high malondialdehyde at PN2 and cathepsin levels at PN14, 21 and 28 in rd1 retinas indicate that cells of the residual inner retina also contribute to the oxidative stress and retinal degeneration.
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| 2. |
- Bautista, Rocío, et al.
(författare)
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Toward a Pinus pinaster bacterial artificial chromosome library
- 2007
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Ingår i: Annals of Forest Science. - : Springer Science and Business Media LLC. - 1286-4560 .- 1297-966X. ; 64:8, s. 855-864
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Tidskriftsartikel (refereegranskat)abstract
- Conifers are of great economic and ecological importance, but little is known concerning their genomic organization. This study is an attempt to obtain high-quality high-molecular-weight DNA from Pinus pinaster cotyledons and the construction of a pine BAC library. The preparation incorporates modifications like low centrifugation speeds, increase of EDTA concentration for plug maintenance, use of DNase inhibitors to reduce DNA degradation, use of polyvinylpyrrolidone and ascorbate to avoid secondary metabolites, and a brief electrophoresis of the plugs prior to their use. A total of 72 192 clones with an average insert size of 107 kb, which represents an equivalent of 11X pine haploid genomes, were obtained. The proportions of clones lacking inserts or containing chloroplast DNA are both approximately 1.6%. The library was screened with cDNA probes for seven genes, and two clones containing Fd-GOGAT sequences were found, one of them seemingly functional. Ongoing projects aimed at constructing a pinebacterial artificial chromosome library may benefit from the methods described here.
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| 3. |
- Cañas, Rafael A, et al.
(författare)
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Molecular and functional analyses support a role of Ornithine-{delta}-aminotransferase in the provision of glutamate for glutamine biosynthesis during pine germination
- 2008
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Ingår i: Plant Physiology. - : Oxford University Press (OUP). - 0032-0889 .- 1532-2548. ; 148:1, s. 77-88
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Tidskriftsartikel (refereegranskat)abstract
- We report the molecular characterization and functional analysis of a gene (PsdeltaOAT) from Scots pine (Pinus sylvestris) encoding Orn-delta-aminotransferase (delta-OAT; EC 2.6.1.13), an enzyme of arginine metabolism. The deduced amino acid sequence contains a putative N-terminal signal peptide for mitochondrial targeting. The polypeptide is similar to other delta-OATs from plants, yeast, and mammals and encoded by a single-copy gene in pine. PsdeltaOAT encodes a functional delta-OAT as determined by expression of the recombinant protein in Escherichia coli and analysis of the active enzyme. The expression of PsdeltaOAT was undetectable in the embryo, but highly induced at early stages of germination and seedling development in all different organs. Transcript levels decreased in later developmental stages, although an increase was observed in lignified stems of 90-d-old plants. An increase of delta-OAT activity was observed in germinating embryos and seedlings and appears to mirror the observed alterations in PsdeltaOAT transcript levels. Similar expression patterns were also observed for genes encoding arginase and isocitrate dehydrogenase. Transcripts of PsdeltaOAT and the arginase gene were found widely distributed in different cell types of pine organs. Consistent with these results a metabolic pathway is proposed for the nitrogen flow from the megagametophyte to the developing seedling, which is also supported by the relative abundance of free amino acids in embryos and seedlings. Taken together, our data support that delta-OAT plays an important role in this process providing glutamate for glutamine biosynthesis during early pine growth.
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| 4. |
- Agner, Tove, et al.
(författare)
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Contact sensitisation in hand eczema patients-relation to subdiagnosis, severity and quality of life: a multi-centre study
- 2009
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Ingår i: Contact Dermatitis. - 0105-1873. ; 61:5, s. 291-296
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Tidskriftsartikel (refereegranskat)abstract
- Background Contact sensitisation has been identified as a factor associated with poor prognosis for patients with hand eczema. Objectives To study implications of contact sensitisation with respect to severity, quality of life (QoL) and subdiagnosis of hand eczema. Methods The study was performed as a multi-centre, cross-sectional study from 10 European clinics. All patients were patch tested, and severity of hand eczema assessed by Hand Eczema Severity Index. A multi-variate analysis was performed to explore which factors influenced severity, QoL and sick leave. Results A total 416 patients were included, and 63% had contact sensitisation to one or more of the tested allergens. More women (66%) than men (51%) were sensitized. No significant association was found between sensitisation to specific allergens, disease severity, QoL or diagnostic subgroups. High age, male sex, atopic eczema and presence of contact sensitisation were independent risk factors for increased severity as measured by Hand Eczema Severity Index. Furthermore, the severity of hand eczema increased by the number of contact sensitisations detected (P = 0.023). High age and personal history of atopic eczema were independent risk factors for low QoL, as measured by Dermatology Life Quality Index, and atopic eczema as well as allergic contact dermatitis as subdiagnosis was associated with increased sick leave. Conclusion Diagnostic subgroups were not found to be related to specific allergens. Contact sensitisation was found to be a risk factor for increased severity of hand eczema, as did high age, male sex and atopic eczema.
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| 5. |
- Agner, Tove, et al.
(författare)
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Hand eczema severity and quality of life: a cross-sectional, multicentre study of hand eczema patients
- 2008
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Ingår i: Contact Dermatitis. - : Wiley. - 0105-1873 .- 1600-0536. ; 59:1, s. 43-47
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Tidskriftsartikel (refereegranskat)abstract
- Background and Objectives: Hand eczema is a chronic disease with negative impact on quality of life (QoL). In this study, QoL in hand eczema patients is assessed and related to age, sex, severity, and diagnostic subgroups. Methods: A total of 416 patients with hand eczema from 10 European patch test clinics participated in the study. Data on QoL were obtained from a self-administered questionnaire using the Dermatology Life Quality Index (DLQI). Severity was assessed by a scoring system (Hand Eczema Severity Index, HECSI) as well as frequency of eruptions and sick leave due to hand eczema. Results: No significant difference was found between males and females with respect to QoL [DLQI median values and 25/75 percentiles for males and females being 7.0 (3-14) and 8.0 (3-13), respectively], although males were more severely affected than females (P < 0.025). A significant positive correlation was found for hand eczema severity and age (P < 0.001), while no significant correlation was found for QoL and age. QoL was found increasingly reduced when sick leave was getting higher (P < 0.001). A statistically significant correlation between QoL (as measured by DLQI) and hand eczema severity as measured by HECSI was found (P < 0.001). No significant difference in QoL was found between diagnostic subgroups. Conclusions: QoL was found markedly negatively affected in hand eczema patients and was significantly correlated to disease severity. No significant difference in QoL was found between males and females, in spite of significantly more severe eczema in males, indicating that QoL in female patients is more easily affected.
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| 6. |
- Agudo, Marta, et al.
(författare)
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Immediate Upregulation of Proteins Belonging to Different Branches of the Apoptotic Cascade in the Retina after Optic Nerve Transection and Optic Nerve Crush
- 2009
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Ingår i: Investigative Ophthalmology and Visual Science. - : Association for Research in Vision and Ophthalmology (ARVO). - 0146-0404 .- 1552-5783. ; 50:1, s. 424-431
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Tidskriftsartikel (refereegranskat)abstract
- Purpose: To further investigate the molecular signals underlying optic nerve (ON) injury we have analyzed in adult control, ON transected and ON crushed retinas, the expression pattern and time-course regulation of the following proteins, all of which are linked to apoptosis through different pathways: Stat 1, Caspase 11 (inflammation and death), Cathepsins C and B (lysosomal death pathway), Calpain 1 (endoplasmic reticulum stress), Calreticulin (apoptosis marker), Jun (early response) and Ahr (cell cycle arrest). Methods: Adult female rats were subjected to either intraorbital optic nerve transection (IONT) or intraorbital optic nerve crush (IONC). Protein from naive and ON injured adult rat retinas was extracted at increasing time-points post-lesion and western blotting experiments carried out. For immnuhistofluorescence analyses, retinal ganglion cells (RGCs) were retrogradelly identified with fluorogold applied to the superior colliculi one week before injury. Results: Western blotting analyses revealed up-regulation of all the analyzed proteins as soon as 12 hours post-lesion (hpl) peaking at 48hpl, in agreement with our previous RNA studies1. Furthermore, immunohistofluorescence to radial sections show that all of them, but Stat1, are expressed by the primarily injured neurons, the RGCs, as seen by colocalization with FG. Conclusions: All analyzed proteins were up-regulated in the retina after IONT or IONC as soon as 12hpl, indicating that ON injury regulates several branches of the apoptotic cascade and suggesting that commitment to death might be an earlier event than previously anticipated.
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| 7. |
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| 8. |
- Alpizar, Francisco, et al.
(författare)
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Anonymity, Reciprocity, and Conformity: Evidence from Voluntary Contributions to a National Park in Costa Rica
- 2007
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- We investigate the role of anonymity, reciprocity, and conformity for voluntary contributions, based on a natural field experiment conducted at a national park in Costa Rica. Contributions made in public in front of the solicitor are 25% higher than contributions made in private. Giving subjects a small gift before requesting a contribution increases the likelihood of a positive contribution. At the same time, the conditional contribution decreases. The total effect of giving a gift is positive but small, and taking the cost of the gift into account, it is far from profitable. When the subjects are told that the typical contribution of others is $2 (a small contribution), the probability of a contribution increases and the conditional contribution decreases, compared with providing no reference information. Providing a high reference level ($10) increases the conditional contributions. Overall, the total effects have the expected signs, although the magnitudes are smaller than what one might have expected based on existing evidence from laboratory experiments.
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| 9. |
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| 10. |
- Alpizar, Francisco, 1974, et al.
(författare)
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Does context matter more for hypothetical than for actual contributions? Evidence from a natural field experiment
- 2007
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- We investigate the importance of the social context for people’s voluntary contributions to a national park in Costa Rica, using a natural field experiment. Some subjects make actual contributions while others state their hypothetical contribution. Both the degree of anonymity and provided information about the contributions of others influence subject contributions in the hypothesized direction. We do find a substantial hypothetical bias with regard to the amount contributed. However, the influence of the social contexts is about the same when the subjects make actual monetary contributions as when they state their hypothetical contributions. Our results have important implications for validity testing of stated preference methods: a comparison between hypothetical and actual behavior should be done for a given social context.
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| 11. |
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| 12. |
- Alpizar, Francisco, 1974, et al.
(författare)
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How Much Do We Care About Absolute versus Relative Income and Consumption?
- 2005
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Ingår i: Journal of Economic Behavior and Organization. - : Elsevier BV. - 0167-2681. ; 56, s. 405-421
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Tidskriftsartikel (refereegranskat)abstract
- We find, using survey-experimental methods, that most individuals are concerned with both relative income and relative consumption of particular goods. The degree of concern varies in the expected direction depending on the properties of the good. However, contrary to what has been suggested in the previous literature, we find that relative consumption is also important for vacation and insurance, which are typically seen as non-positional goods. Further, absolute consumption is also found to be important for cars and housing, which are widely regarded as highly positional. © 2004 Published by Elsevier B.V.
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| 13. |
- Alpizar, Francisco, 1974, et al.
(författare)
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The effect of risk, ambiguity, and coordination on farmers’ adaptation to climate change: A framed field experiment
- 2009
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- The risk of loses of income and productive means due to adverse weather associated to climate change can significantly differ between farmers sharing a productive landscape. It is important to learn more about how farmers react to different levels of risk, under measurable and unmeasurable uncertainty. Moreover, the costs associated to investments in reduced vulnerability to climatic events are likely to exhibit economies of scope. We explore these issues using a framed field experiment that captures realistically the main characteristics of production, and the likely weather related loses of premium coffee farmers in Tarrazu, Costa Rica. Given that the region recently was severely hit by an extreme, albeit very infrequent, climatic event, we expected to observe, and found high levels of risk aversion, but we do observe farmers making trade offs under different risk levels. Although hard to disentangle at first sight given the high level of risk aversion, we find that farmer’s opt more frequently for safe options in a setting characterized by unknown risk. Finally, we find that farmers to a large extent are able to coordinate their decisions in order to achieve a lower cost of adaptation, and that communication among farmers strongly facilitates coordination.
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| 14. |
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| 15. |
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| 16. |
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| 17. |
- Arraigada, M, et al.
(författare)
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Evaluation of Accelerometers to Determine Pavement Deflections under Traffic Loads
- 2008
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Ingår i: Materials and Structures. - : Springer Science and Business Media LLC. - 1359-5997 .- 1871-6873. ; 42:6, s. 779-790
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Tidskriftsartikel (refereegranskat)abstract
- The purpose of this work was to study the use of accelerometers to measure pavement deflections due to traffic loads. To this end, accelerometers were embedded in two sites: the full scale load simulator Circular Test Track (CTT) and the A1 motorway in Switzerland. Deflections were derived from acceleration measurements using an algorithm that double integrates the measured signal and corrects any errors derived from the procedure. In the motorway, deflections were monitored using a set of three magnetostrictive deflectometers. Additionally, the pavement's material viscoelastic parameters determined in the laboratory were incorporated in Finite Element (FE) models to estimate the theoretical deflections. The calculated deflections were then compared to the measured and to the theoretical deflections. Deflections calculated from acceleration showed a reasonable qualitative correlation to those measured by magnetostrictive deflectometers. In addition, the FE models revealed the inability of the accelerometers to measure very slow or quasi-static motion.
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| 18. |
- Arriaga, Patricia, et al.
(författare)
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Are the effects of unreal violent video games pronounced when playing with a virtual reality system?
- 2008
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Ingår i: Aggressive Behavior. - : Wiley. - 0096-140X .- 1098-2337. ; 34:5, s. 521-538
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Tidskriftsartikel (refereegranskat)abstract
- This study was conducted to analyze the short‐term effects of violent electronic games, played with or without a virtual reality (VR) device, on the instigation of aggressive behavior. Physiological arousal (heart rate (HR)), priming of aggressive thoughts, and state hostility were also measured to test their possible mediation on the relationship between playing the violent game (VG) and aggression. The participants—148 undergraduate students—were randomly assigned to four treatment conditions: two groups played a violent computer game (Unreal Tournament), and the other two a non‐violent game (Motocross Madness), half with a VR device and the remaining participants on the computer screen. In order to assess the game effects the following instruments were used: a BIOPAC System MP100 to measure HR, an Emotional Stroop task to analyze the priming of aggressive and fear thoughts, a self‐report State Hostility Scale to measure hostility, and a competitive reaction‐time task to assess aggressive behavior. The main results indicated that the violent computer game had effects on state hostility and aggression. Although no significant mediation effect could be detected, regression analyses showed an indirect effect of state hostility between playing a VG and aggression.
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| 19. |
- Arriaga, Patricia, et al.
(författare)
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Violent computer games and their effects on state hostility and psychophysiological arousal
- 2006
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Ingår i: Aggressive Behavior. - : Wiley. - 0096-140X .- 1098-2337. ; 32:2, s. 146-158
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Tidskriftsartikel (refereegranskat)abstract
- An experimental study was conducted to investigate the impact of violent computer games on state hostility, state anxiety and arousal. Participants were undergraduate students, aged from 18 to 25 years. Before the experimental sessions, participants filled in self-report measures concerning their video game habits and were also pre-tested for aggressiveness and trait anxiety. Physiological responses (heart rate and skin conductance) were measured during the experiment. After playing, information about state hostility and state anxiety was collected. The results showed that participants who played the violent game reported significantly higher state hostility, and support the assumption that an aggressive personality moderates the effect of playing a violent game on state hostility.
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| 20. |
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| 21. |
- Baccarani, Michele, et al.
(författare)
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Chronic myeloid leukemia : an update of concepts and management recommendations of European LeukemiaNet
- 2009
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Ingår i: Journal of Clinical Oncology. - 0732-183X .- 1527-7755. ; 27:35, s. 6041-6051
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Forskningsöversikt (refereegranskat)abstract
- PURPOSE: To review and update the European LeukemiaNet (ELN) recommendations for the management of chronic myeloid leukemia with imatinib and second-generation tyrosine kinase inhibitors (TKIs), including monitoring, response definition, and first- and second-line therapy. METHODS: These recommendations are based on a critical and comprehensive review of the relevant papers up to February 2009 and the results of four consensus conferences held by the panel of experts appointed by ELN in 2008. RESULTS: Cytogenetic monitoring was required at 3, 6, 12, and 18 months. Molecular monitoring was required every 3 months. On the basis of the degree and the timing of hematologic, cytogenetic, and molecular results, the response to first-line imatinib was defined as optimal, suboptimal, or failure, and the response to second-generation TKIs was defined as suboptimal or failure. CONCLUSION: Initial treatment was confirmed as imatinib 400 mg daily. Imatinib should be continued indefinitely in optimal responders. Suboptimal responders may continue on imatinb, at the same or higher dose, or may be eligible for investigational therapy with second-generation TKIs. In instances of imatinib failure, second-generation TKIs are recommended, followed by allogeneic hematopoietic stem-cell transplantation only in instances of failure and, sometimes, suboptimal response, depending on transplantation risk.
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| 22. |
- Baccarani, Michele, et al.
(författare)
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Evolving concepts in the management of chronic myeloid leukemia : recommendations from an expert panel on behalf of the European LeukemiaNet
- 2006
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Ingår i: Blood. - : American Society of Hematology. - 0006-4971 .- 1528-0020. ; 108:6, s. 1809-1820
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Tidskriftsartikel (refereegranskat)abstract
- The introduction of imatinib mesylate (IM) has revolutionized the treatment of chronic myeloid leukemia (CML). Although experience is too limited to permit evidence-based evaluation of survival, the available data fully justify critical reassessment of CML management. The panel therefore reviewed treatment of CML since 1998. It confirmed the value of IM (400 mg/day) and of conventional allogeneic hematopoietic stem cell transplantation (alloHSCT). It recommended that the preferred initial treatment for most patients newly diagnosed in chronic phase should now be 400 mg IM daily. A dose increase of IM, alloHSCT, or investigational treatments were recommended in case of failure, and could be considered in case of suboptimal response. Failure was defined at 3 months (no hematologic response [HR]), 6 months (incomplete HR or no cytogenetic response [CgR]), 12 months (less than partial CgR [Philadelphia chromosome-positive (Ph(+)) > 35%]), 18 months (less than complete CgR), and in case of HR or CgR loss, or appearance of highly IM-resistant BCR-ABL mutations. Suboptimal response was defined at 3 months (incomplete HR), 6 months (less than partial CgR), 12 months (less than complete CgR), 18 months (less than major molecular response [MMolR]), and, in case of MMolR loss, other mutations or other chromosomal abnormalities. The importance of regular monitoring at experienced centers was highlighted.
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| 23. |
- Badia, J. D., et al.
(författare)
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Thermal analysis as a quality tool for assessing the influence of thermo-mechanical degradation on recycled poly(ethylene terephthalate)
- 2009
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Ingår i: Polymer testing. - : Elsevier BV. - 0142-9418 .- 1873-2348. ; 28:2, s. 169-175
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Tidskriftsartikel (refereegranskat)abstract
- Mechanical recycling of poly(ethylene terephthalate) (PET) was simulated by multiple processing to assess the effects of thermo-mechanical degradation, and characterized using rheological and thermal analysis techniques. Thermo-mechanical degradation under repeated extrusion induces chain scission reactions in PET, which result in a dramatic loss in the deformation capabilities and an increase in the fluidity of the polymer under reprocessing, reducing its recycling possibilities after four extrusion cycles. Multiple reprocessing severely affects the storage modulus and the microstructure of recycled PET, both in the amorphous and crystalline regions. Multimodal melting behavior is observed for reprocessed PET, indicating heterogeneous and segregated crystalline regions. A deconvolution procedure has been applied to individually characterize each crystalline population in terms of lamellar thickness distribution and partial crystallinity. Thermal analysis techniques such as differential scanning calorimetry (DSC) and dynamic-mechanical analysis (DMA) have proved to be suitable techniques for the quality assessment of recycled PET, giving unequivocal information about its degree of degradation compared to the common technological measurements of melt-mass flow rate (MFR) or oxidative stability (T-OX).
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| 24. |
- Bassand, Jean-Pierre, et al.
(författare)
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Guía de Práctica Clínica para el diagnóstico y tratamiento del síndrome coronario agudo sin elevación del segmento ST
- 2007
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Ingår i: Revista Española de Cardiología. - 0300-8932 .- 1579-2242. ; 60:10, s. 1070-1080
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Tidskriftsartikel (refereegranskat)abstract
- El contenido de estas Guías de Práctica Clínica de la Sociedad Europea de Cardiología (ESC) ha sido publicado para uso exclusivamente personal y educativo. No está autorizado su uso comercial. No se permite la traducción o reproducción en ningún formato de las Guías de la ESC ni de ninguna de sus partes sin un permiso escrito de la ESC. El permiso puede obtenerse enviando una solicitud por escrito a Oxford University Press, la editorial del European Heart Journal, y parte autorizada para gestionar esos permisos en representación de la ESC.
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| 25. |
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| 26. |
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| 27. |
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| 28. |
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| 29. |
- Bengtsson, Göran, et al.
(författare)
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Mercury sorption to sediments: Dependence on grain size, dissolved organic carbon, and suspended bacteria.
- 2008
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Ingår i: Chemosphere. - : Elsevier BV. - 1879-1298 .- 0045-6535. ; 73:4, s. 526-531
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Tidskriftsartikel (refereegranskat)abstract
- A combination of laboratory scale derived correlations and measurements of grain size distribution, DOC (dissolved organic carbon) concentration, and density of suspended bacteria promises to be useful in estimating Hg(II) sorption in heterogeneous streambeds and groundwater environments. This was found by shaking intact sediment and fractions thereof (<63-2000mum) with solutions of HgCl(2) (1.0-10.0ngml(-1)). The intact sediment was also shaken with the Hg(II) solutions separately in presence of DOC (6.5-90.2mugml(-1)) or brought in contact with suspensions of a strain of groundwater bacteria (2x10(4)-2x10(6)cellsml(-1)). Hg(II) sorption was rather weak and positively correlated with the grain size, and the sorption coefficient (K(d)) varied between about 300 and 600mlg(-1). By using the relative surface areas of the fractions, K(d) for the intact sediment was back calculated with 2% deviation. K(d) was negatively correlated with the concentration of DOC and positively correlated with the number of bacteria. A multiple regression showed that K(d) was significantly more influenced by the number of bacteria than by the grain size. The findings imply that common DOC concentrations in groundwater and streambeds, 5-20mugml(-1), will halve the K(d) obtained from standard sorption assays of Hg(II), and that K(d) will almost double when the cell numbers are doubled at densities that are common in aquifers. The findings suggest that simultaneous measurements of surface areas of sediment particles, DOC concentrations, and bacterial numbers are useful to predict spatial variation of Hg(II) sorption in aquifers and sandy sediments.
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| 30. |
- Benito, Natividad, et al.
(författare)
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Health care-associated native valve endocarditis: importance of non-nosocomial acquisition.
- 2009
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Ingår i: Annals of internal medicine. - : American College of Physicians. - 1539-3704 .- 0003-4819. ; 150:9, s. 586-94
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Tidskriftsartikel (refereegranskat)abstract
- The clinical profile and outcome of nosocomial and non-nosocomial health care-associated native valve endocarditis are not well defined.To compare the characteristics and outcomes of community-associated and nosocomial and non-nosocomial health care-associated native valve endocarditis.Prospective cohort study.61 hospitals in 28 countries.Patients with definite native valve endocarditis and no history of injection drug use who were enrolled in the ICE-PCS (International Collaboration on Endocarditis Prospective Cohort Study) from June 2000 to August 2005.Clinical and echocardiographic findings, microbiology, complications, and mortality.Health care-associated native valve endocarditis was present in 557 (34%) of 1622 patients (303 with nosocomial infection [54%] and 254 with non-nosocomial infection [46%]). Staphylococcus aureus was the most common cause of health care-associated infection (nosocomial, 47%; non-nosocomial, 42%; P = 0.30); a high proportion of patients had methicillin-resistant S. aureus (nosocomial, 57%; non-nosocomial, 41%; P = 0.014). Fewer patients with health care-associated native valve endocarditis had cardiac surgery (41% vs. 51% of community-associated cases; P < 0.001), but more of the former patients died (25% vs. 13%; P < 0.001). Multivariable analysis confirmed greater mortality associated with health care-associated native valve endocarditis (incidence risk ratio, 1.28 [95% CI, 1.02 to 1.59]).Patients were treated at hospitals with cardiac surgery programs. The results may not be generalizable to patients receiving care in other types of facilities or to those with prosthetic valves or past injection drug use.More than one third of cases of native valve endocarditis in non-injection drug users involve contact with health care, and non-nosocomial infection is common, especially in the United States. Clinicians should recognize that outpatients with extensive out-of-hospital health care contacts who develop endocarditis have clinical characteristics and outcomes similar to those of patients with nosocomial infection.None.
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| 31. |
- Berglund, Ann-Charlotte, et al.
(författare)
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Combining local and grid resources in scientific workflows (for Bioinformatics)
- 2009
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Konferensbidrag (refereegranskat)abstract
- We examine some issues that arise when using both local and Gridresources in scientific workflows. Our previous work addresses and illustratesthe benefits of a light-weight and generic workflow engine that manages andoptimizes Grid resource usage. Extending on this effort, we hereillustrate how a client tool for bioinformatics applications employs the engine tointerface with Grid resources. We also explore how to define data flowsthat transparently integrates local and Grid subworkflows. In addition, the benefits of parameter sweep workflows are examined and a means for describing this type of workflows in an abstract and concise manner is introduced. Finally, the above mechanisms are employed to perform an orthology detection analysis.
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| 32. |
- Berthold, Jessica, et al.
(författare)
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Characterization of RhoBTB-dependent Cul3 ubiquitin ligase complexes--evidence for an autoregulatory mechanism
- 2008
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Ingår i: Experimental Cell Research. - : Elsevier BV. - 0014-4827 .- 1090-2422. ; 314:19, s. 3453-3465
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Tidskriftsartikel (refereegranskat)abstract
- RhoBTB proteins are atypical members of the Rho family of small GTPases. Two of the three RhoBTB proteins, RhoBTB1 and RhoBTB2, have been proposed as tumor suppressors and might function as adaptors of Cul3-dependent ubiquitin ligase complexes. Using yeast two-hybrid analysis and co-immunoprecipitation we show that all three RhoBTB proteins interact with Cul3. The interaction requires the N-terminal region of Cul3 and the first BTB domain of RhoBTB. RhoBTB3, the only RhoBTB with a prenylation motif, associates with vesicles that are frequently found in the vicinity of microtubules, suggesting a participation in some aspects of vesicle trafficking. We also show that RhoBTB2 and RhoBTB3 are capable of homo and heterodimerizing through the BTB domain region. The GTPase domain, which does not bind GTP, is able to interact with the BTB domain region, thus preventing proteasomal degradation of RhoBTB. This fits into a model in which an intramolecular interaction maintains RhoBTB in an inactive state, preventing the formation or the functionality of Cul3-dependent complexes. We also report a significantly decreased expression of RHOBTB and CUL3 genes in kidney and breast tumor samples and a very good correlation in the expression changes between RHOBTB and CUL3 that suggests that these genes are subject to a common inactivation mechanism in tumors.
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| 33. |
- Birney, Ewan, et al.
(författare)
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Prepublication data sharing
- 2009
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 461:7261, s. 168-170
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Tidskriftsartikel (refereegranskat)abstract
- Rapid release of prepublication data has served the field of genomics well. Attendees at a workshop in Toronto recommend extending the practice to other biological data sets.
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| 34. |
- Bispo-Santos, Franklin, et al.
(författare)
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Columbia revisited : paleomagnetic results from the 1790 Ma colider volcanics (SW Amazonian Craton, Brazil)
- 2008
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Ingår i: Precambrian Research. - : Elsevier BV. - 0301-9268 .- 1872-7433. ; 164:1-2, s. 40-49
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Tidskriftsartikel (refereegranskat)abstract
- In an attempt to improve our understanding of the Paleoproterozoic geodynamic evolution, a paleomagnetic study was performed on 10 sites of acid volcanic rocks of the Colider Suite, southwestern Amazonian Craton. These rocks have a well-dated zircon U-Pb mean age of 1789 +/- 7 Ma. Alternating field and thermal demagnetization revealed northern (southern) directions with moderate to high upward (downward) inclinations. Rock magnetism experiments and magnetic mineralogy show that this characteristic magnetization is carried by Ti-poor magnetite or by hematite that replaces magnetite by late-magmatic cleuteric alteration. Both magnetite and hematite carry the same characteristic component. The mean direction (Dm = 183.0 degrees, Im = 53.5 degrees, N = 10, alpha(95) = 9.8 degrees, K = 25.2) yielded a paleomagnetic pole located at 298.8 degrees E, 63.3 degrees S (alpha(95) = 10.2 degrees, K = 23.6), which is classified with a quality factor Q = 5. Paleogeographic reconstructions using this pole and other reliable Paleoproterozoic poles suggest that Laurentia, Baltica, North China Craton and Amazonian Craton were located in laterally contiguous positions forming a large continental mass at 1790 Ma ago. This is reinforced by geological evidence which support the existence of the supercontinent Columbia in Paleoproterozoic times.
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| 35. |
- Bjursäter, Ulla, et al.
(författare)
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On Linguistic and Interactive Aspects of Infant-Adult Communication in a Pathological Perspective
- 2005
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Ingår i: Proceedings, FONETIK 2005. - Göteborg : Göteborgs universitet, Institutionen för lingvistik. ; , s. 55-58
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Konferensbidrag (refereegranskat)abstract
- This is a preliminary report of a study of some linguistic and interactive aspects available in an adult-child dyad where the child is partially hearing impaired, during the ages 8 - 20 months. The investigation involves a male child, born with Hemifacial Microsomia. Audio and video recordings are used to collect data on child vocalization and parent-child interaction. Eye-tracking is used to measure eye movements when presented with audio-visual stimuli. SECDI forms are applied to observe the development of the child's lexical production. Preliminary analyses indicate increased overall parental interactive behaviour. As babbling is somewhat delayed due to physical limitations, signed supported Swedish is used to facilitate communication and language development. Further collection and analysis of data is in progress in search of valuable information of the linguistic development from a pathological perspective of language acquisition.
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| 36. |
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| 37. |
- Björkin, Mats, 1967, et al.
(författare)
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European Television Audiences: Localising the Viewers
- 2008
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Ingår i: A European Television History , Jonathan Bignell &Andreas Fickers (eds). - Oxford : Wiley-Blackwell. - 9781405163392 ; , s. 221-228
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 38. |
- Broeke, Ronald G., et al.
(författare)
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Optical-CDMA in InP
- 2007
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Ingår i: IEEE Journal of Selected Topics in Quantum Electronics. - : Institute of Electrical and Electronics Engineers (IEEE). - 1077-260X .- 1558-4542. ; 13:5, s. 1497-1507
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Tidskriftsartikel (refereegranskat)abstract
- This paper describes the InP platforms for photonic integration and the development on these platforms of an optical code division multiple access (O-CDMA) system for local area networks. We demonstrate three building blocks of this system: an optical pulse source, an encoder/decoder pair, and a threshold detector. The optical pulse source consists of an integrated colliding pulse-mode laser with nearly transform-limited 10 Gb/s pulses and optical injection locking to an external clock for synchronization. The encoder/decoder pair is based on arrayed waveguide gratings. Bit-error-rate measurements involving six users at 10 Gb/s showed error-free transmission, while O-CDMA codes were calibrated using frequency resolved optical gating. For threshold detection after the decoder, we compared two Mach-Zehnder interferometer (MZI)-based optical thresholding schemes and present results on a new type of electroabsorber-based MZI.
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| 39. |
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| 40. |
- Calvo, Manuel, et al.
(författare)
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Detection of emotional faces : low perceptual thresholds and wide attentional span
- 2005
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Ingår i: Visual cognition (Print). - : Informa UK Limited. - 1350-6285 .- 1464-0716. ; 12:1, s. 13-27
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Tidskriftsartikel (refereegranskat)abstract
- In two experiments, prime face stimuli with an emotional or a neutral expression were presented individually for 25 to 125 ms, either in foveal or parafoveal vision; following a mask, a probe face or a word label appeared for recognition. Accurate detection and sensitivity (A') were higher for angry, happy, and sad faces than for nonemotional (neutral) or novel (scheming) faces at short exposure times (25-75 ms), in both the foveal and the parafoveal field, and with both the probe face and the probe word. These results indicate that there is a low perceptual threshold for unambiguous emotional faces, which are especially likely to be detected both within and outside the focus of attention; and that this facilitated detection involves processing of the affective meaning of faces, not only discrimination of formal visual features.
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| 41. |
- Capella, Gabriel, et al.
(författare)
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DNA repair polymorphisms and the risk of stomach adenocarcinoma and severe chronic gastritis in the EPIC-EURGAST study
- 2008
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Ingår i: International Journal of Epidemiology. - : Oxford University Press (OUP). - 1464-3685 .- 0300-5771. ; 37:6, s. 1316-1325
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Tidskriftsartikel (refereegranskat)abstract
- Background The contribution of genetic variation in DNA repair genes to gastric cancer (GC) risk remains essentially unknown. The aim of this study was to explore the relative contribution of DNA repair gene polymorphisms to GC risk and severe chronic atrophic gastritis (SCAG). Method A nested case control study within the EPIC cohort was performed including 246 gastric adenocarcinomas and 1175 matched controls. Controls with SCAG (n 91), as defined by low pepsinogen A (PGA) levels, and controls with no SCAG (n 1061) were also compared. Twelve polymorphisms at DNA repair genes (MSH2, MLH1, XRCC1, OGG1 and ERCC2) and TP53 gene were analysed. Antibodies against Helicobacter pylori were measured. Results No association was observed for any of these polymorphisms with stomach cancer risk. However, ERCC2 K751Q polymorphism was associated with an increased risk for non-cardial neoplasm [odds ratio (OR) 1.78; 95 confidence interval (CI) 1.023.12], being ERCC2 K751Q and D312N polymorphisms associated with the diffuse type. ERCC2 D312N (OR 2.0; 95 CI 1.093.65) and K751Q alleles (OR 1.82; 95 CI 1.013.30) and XRCC1 R399Q (OR 1.69; 95 CI 1.022.79) allele were associated with an increased risk for SCAG. Conclusion Our study supports a role of ERCC2 in non-cardial GC but not in cardial cancer. A concordant result was observed for subjects with low PGA levels. XRCC1 allele was associated also with SCAG. This is the first prospective study suggesting that individual variation in DNA repair may be relevant for gastric carcinogenesis, a finding that will require further confirmation validation in larger independent studies.
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| 42. |
- Cardol, Pierre, et al.
(författare)
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Oxidative Phosphorylation : Building blocks and related components
- 2009
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Ingår i: The Chlamydomonas Sourcebook. - Oxford : Academic Press. - 0123708753 ; , s. 469-502
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- This chapter summarizes the knowledge of the oxidative phosphorylation (OXPHOS) constituents of Chlamydomonas and of the components involved in their biogenesis and addresses alternate dehydrogenases and oxidases which are particular to photosynthetic organisms, and several other mitochondrial components related to OXPHOS. Reference to the components of Polytomella sp., a colorless alga closely related to Chlamydomonas is clearly made. The main complexes involved in electron transport seem to share a similar number of subunits, and many of the algal polypeptides have plant homologues. Some differences are apparent, such as the presence of a fragmented COX2 subunit, which seems to be unique to chlorophyte algae. OXPHOS is defined as an electron transfer chain driven by substrate oxidation that is coupled to the synthesis of ATP through an electrochemical transmembrane gradient. The characterization of Arabidopsis mitochondrial components through proteomic approaches has advanced significantly. As a unicellular organism, Chlamydomonas offers the unique opportunity to study organelle-organelle interactions, particularly between mitochondria and chloroplasts. It has become evident that crosstalk between these organelles takes place, mainly through intracellular metabolite pools. © 2009 Elsevier Inc. All rights reserved.
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| 43. |
- Carneiro, Paula, et al.
(författare)
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Analyzing false memories in children with associative lists specific for their age
- 2007
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Ingår i: Child Development. - : Wiley. - 0009-3920 .- 1467-8624. ; 78:4, s. 1171-1185
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Tidskriftsartikel (refereegranskat)abstract
- Two experiments attempted to resolve previous contradictory findings concerning developmental trends in false memories within the Deese-Roediger-McDermott (DRM) paradigm by using an improved methodology--constructing age-appropriate associative lists. The research also extended the DRM paradigm to preschoolers. Experiment 1 (N=320) included children in three age groups (preschoolers of 3-4 years, second-graders of 7-8 years, and preadolescents of 11-12 years) and adults, and Experiment 2 (N=64) examined preschoolers and preadolescents. Age-appropriate lists increased false recall. Although preschoolers had fewer false memories than the other age groups, they showed considerable levels of false recall when tested with age-appropriate materials. Results were discussed in terms of fuzzy-trace, source-monitoring, and activation frameworks.
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| 44. |
- Carracedo, Juan Carlos, et al.
(författare)
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Reply to Comment on “Recent unrest at Canary Islands' Teide Volcano?”
- 2007
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Ingår i: EOS. - 0096-3941 .- 2324-9250. ; 88:46, s. 488-
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Small-magnitude seismic episodes unrelated to a volcanic eruption have been a relatively frequent feature in all the Canaries without causing any significant public alarm. Conversely, great alarm was raised in May 2004 in Tenerife, when apparently numerous low-magnitude seismic signals were recorded, although only a few of them were actually felt in nearby villages.Public alarm was raised by (1) the publication on a Web site of imperceptible seismic signals as low as 0.6 on the Richter scale, most of which were not even adequately localized and yet were reproduced almost daily in the local and national press without further comment or explanation; (2) a Spanish national scientific committee being replaced by a local committee that was scientifically advised by a private company; and (3) publicity given by the media to the prediction made by members of the local committee of a potentially large scale explosive eruption in October 2004 (dubbed “the October volcano” by residents). Interestingly, obvious fumarole activity was absent in autumn 2004 during an inspection of Teide summit by three of us.
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| 45. |
- Castells, Francisco, et al.
(författare)
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Principal component analysis in ECG signal processing
- 2007
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Ingår i: Eurasip Journal on Applied Signal Processing. - : Springer Science and Business Media LLC. - 1110-8657. ; , s. 74580-74580
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Tidskriftsartikel (refereegranskat)abstract
- This paper reviews the current status of principal component analysis in the area of ECG signal processing. The fundamentals of PCA are briefly described and the relationship between PCA and Karhunen-Loeve transform is explained. Aspects on PCA related to data with temporal and spatial correlations are considered as adaptive estimation of principal components is. Several ECG applications are reviewed where PCA techniques have been successfully employed, including data compression, ST-T segment analysis for the detection of myocardial ischemia and abnormalities in ventricular repolarization, extraction of atrial fibrillatory waves for detailed characterization of atrial fibrillation, and analysis of body surface potential maps.
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| 46. |
- Cherkin, Daniel, et al.
(författare)
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The Ninth International Forum for Primary Care Research on Low Back Pain
- 2009
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Ingår i: SPINE. - 0362-2436. ; 34:3, s. 304-307
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Tidskriftsartikel (refereegranskat)abstract
- Study Design. Review of oral and poster presentations and of workshop summaries from the Ninth International Forum for Primary Care Research on Low Back Pain held in Majorca, Spain, October 4-6, 2007. Objective. Summarize highlights of the conference. Summary of Background Data. The International Forum for Primary Care Research on Low Back Pain has become the primary conference for presenting research on the advances in primary care for back pain. Methods. Distillation of the key themes and findings of the research presented at the Forum. Results. Presentations at the forum included intervention studies, psychosocial aspects of low back pain, and epidemiological, clinical, and pathologic studies. Conclusion. The research presented at the forum has contributed to the advancement of understanding of how to improve primary care for low back pain. The Tenth International Forum will occur on June 14-17, 2009, in Boston.
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| 47. |
- Coleman, Ross A., et al.
(författare)
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A continental scale evaluation of the role of limpet grazing on rocky shores
- 2006
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Ingår i: OECOLOGIA. - : Springer Science and Business Media LLC. - 0029-8549 .- 1432-1939. ; 147:3, s. 556-564
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Tidskriftsartikel (refereegranskat)abstract
- It is critical for our knowledge of biodiversity and ecosystem processes to understand how individual species contribute to ecosystem processes and how these contributions vary in space and time. We used a manipulative field experiment in five locations over 17 degrees of latitude [from southern Portugal to the Isle of Man (British Isles)] to determine the relative response of rocky intertidal algal assemblages released from control by the grazing of limpets. Response ratios showed that when limpets were removed there was a trend of effects from north to south. In the north, grazing had a strong effect on algal assemblages, but removing grazers reduced spatial variability in assemblages. In the south, the effect of limpet grazing was far weaker and removal of grazers had a much reduced impact on spatial variability. Here we show a clear trophic control of an ecosystem in that grazing by limpets not only determines macroalgal abundance overall but also modifies ecosystem stability via variability in cover of algae.
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| 48. |
- Cortes, Elisabet Eir, 1965-, et al.
(författare)
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From movements to sound Contributions to building the BB speech production system
- 2006
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- In terms of anatomical geometry the infant Vocal Tract undergoes significant change during development. This research note reports an attempt to reconstruct an infant VT from adult data. Comparable landmarks were identified on the fixed structures of adult articulatory lateral profiles (obtained from X-ray images) and matching infant profiles (obtained from published data in the literature, Sobotta [Putz & Pabst 2001, and personal communication from author Prof. Dr. med. R. Pabst]. The x-coordinates of the infant landmarks could be accurately derived by a linear scaling of the adult data whereas the y-values required information on both the x- and the y-coordinates of the adult. These scaling rules were applied to about 400 adult articulatory profiles to derive a set of corresponding infant articulations. A Principal Components Analysis was performed on these shapes to compare the shapes of the infant and adult articulatory spaces. As expected from the scaling results the infant space is significantly compressed in relation to the adult space suggesting that the main articulatory degree of freedom for the child is jaw opening. This finding is in perfect agreement with published descriptions of the phonetics of early vocalizations.
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| 49. |
- Costa, Rui, et al.
(författare)
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Skin conductance responses to visual sexual stimuli
- 2008
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Ingår i: International Journal of Psychophysiology. - : Elsevier BV. - 0167-8760 .- 1872-7697. ; 67:1, s. 64-69
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Tidskriftsartikel (refereegranskat)abstract
- Previous research showed that the asymmetrical direction of bilateral skin conductance responses (SCRs) remains constant regardless of task (with larger left SCRs in men and larger right SCRs in women). However, SCRs are controlled ipsilaterally by structures also associated with sexual arousal, hence it could be expected that larger right SCRs are specifically elicited by sexual stimuli. In order to test the two competing hypotheses, left and right SCR magnitude to three stimulus categories (sexually explicit, sexually non-explicit and neutral) were compared in 54 subjects (27 females). The direction of the asymmetry remained constant across stimulus types, however, unexpected sex differences occurred, as males had larger right SCRs and there was no lateralization in females. Interestingly, this interaction disappeared after controlling for indicators of subjective sexual arousal, suggesting that a specific (not previously hypothesized) processing of sexual information could take place.
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| 50. |
- Coull, Mia, et al.
(författare)
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Kan en robot lära sig att prata?
- 2007
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Annan publikation (populärvet., debatt m.m.)abstract
- Kort notis om forskningsprojekt med robotmodeller av tidig språkutveckling (Vi föräldrar, 2007, Nr 13, s. 73)
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