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- Gruber, Christian W, et al.
(author)
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Distribution and evolution of circular miniproteins in flowering plants
- 2008
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In: The Plant Cell. - : Oxford University Press (OUP). - 1040-4651 .- 1532-298X. ; 20:9, s. 2471-2483
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Journal article (peer-reviewed)abstract
- Cyclotides are disulfide-rich miniproteins with the unique structural features of a circular backbone and knotted arrangement of three conserved disulfide bonds. Cyclotides have been found only in two plant families: in every analyzed species of the violet family (Violaceae) and in few species of the coffee family (Rubiaceae). In this study, we analyzed >200 Rubiaceae species and confirmed the presence of cyclotides in 22 species. Additionally, we analyzed >140 species in related plant families to Rubiaceae and Violaceae and report the occurrence of cyclotides in the Apocynaceae. We further report new cyclotide sequences that provide insights into the mechanistic basis of cyclotide evolution. On the basis of the phylogeny of cyclotide-bearing plants and the analysis of cyclotide precursor gene sequences, we hypothesize that cyclotide evolution occurred independently in various plant families after the divergence of Asterids and Rosids ( approximately 125 million years ago). This is strongly supported by recent findings on the in planta biosynthesis of cyclotides, which involves the serendipitous recruitment of ubiquitous proteolytic enzymes for cyclization. We further predict that the number of cyclotides within the Rubiaceae may exceed tens of thousands, potentially making cyclotides one of the largest protein families in the plant kingdom.
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- Suttrop, W., et al.
(author)
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In-vessel saddle coils for MHD control in ASDEX Upgrade
- 2009
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In: Fusion engineering and design. - Lausanne : ELSEVIER SCIENCE SA. - 0920-3796 .- 1873-7196. ; 84:2-6, s. 290-294
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Journal article (peer-reviewed)abstract
- A set of 24 in-vessel saddle coils is planned for MHD control experiments in ASDEX Upgrade. These coils can produce static and alternating error fields for suppression of Edge Localised Modes, locked mode rotation control and, together with additional conducting wall elements, resistive wall mode excitation and feedback stabilisation experiments. All of these applications address critical physics issues for the operation of ITER. This extension is implemented in several stages, starting with two poloidally separated rings of eight toroidally distributed saddle coils above and below the outer midplane. In stages 2 and 3, eight midplane coils around the large vessel access ports and 12 AC Power converters are added, respectively. Finally (stage 4), the existing passive stabilising loop (PSL), a passive conductor for vertical growth rate reduction, will be complemented by wall elements that allow helical Current patterns to reduce the RWM growth rate for active control within the accessible bandwidth. The system is capable of producing error fields with toroidal mode number n = 4 for plasma edge ergodisation with core island width well below the neo-classical tearing mode seed island width even without rotational shielding. Phase variation between the three toroidal coil rings allows to create or avoid resonances with the plasma safety factor profile, in order to test the importance of resonances for ELM suppression.
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