| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Kim, Jae-Young, et al.
(författare)
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Event Horizon Telescope imaging of the archetypal blazar 3C 279 at an extreme 20 microarcsecond resolution
- 2020
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Ingår i: Astronomy and Astrophysics. - : EDP Sciences. - 0004-6361 .- 1432-0746. ; 640
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Tidskriftsartikel (refereegranskat)abstract
- 3C 279 is an archetypal blazar with a prominent radio jet that show broadband flux density variability across the entire electromagnetic spectrum. We use an ultra-high angular resolution technique - global Very Long Baseline Interferometry (VLBI) at 1.3mm (230 GHz) - to resolve the innermost jet of 3C 279 in order to study its fine-scale morphology close to the jet base where highly variable-ray emission is thought to originate, according to various models. The source was observed during four days in April 2017 with the Event Horizon Telescope at 230 GHz, including the phased Atacama Large Millimeter/submillimeter Array, at an angular resolution of ∼20 μas (at a redshift of z = 0:536 this corresponds to ∼0:13 pc ∼ 1700 Schwarzschild radii with a black hole mass MBH = 8 × 108 M⊙). Imaging and model-fitting techniques were applied to the data to parameterize the fine-scale source structure and its variation.We find a multicomponent inner jet morphology with the northernmost component elongated perpendicular to the direction of the jet, as imaged at longer wavelengths. The elongated nuclear structure is consistent on all four observing days and across diffierent imaging methods and model-fitting techniques, and therefore appears robust. Owing to its compactness and brightness, we associate the northern nuclear structure as the VLBI "core". This morphology can be interpreted as either a broad resolved jet base or a spatially bent jet.We also find significant day-to-day variations in the closure phases, which appear most pronounced on the triangles with the longest baselines. Our analysis shows that this variation is related to a systematic change of the source structure. Two inner jet components move non-radially at apparent speeds of ∼15 c and ∼20 c (∼1:3 and ∼1:7 μas day-1, respectively), which more strongly supports the scenario of traveling shocks or instabilities in a bent, possibly rotating jet. The observed apparent speeds are also coincident with the 3C 279 large-scale jet kinematics observed at longer (cm) wavelengths, suggesting no significant jet acceleration between the 1.3mm core and the outer jet. The intrinsic brightness temperature of the jet components are ≤1010 K, a magnitude or more lower than typical values seen at ≥7mm wavelengths. The low brightness temperature and morphological complexity suggest that the core region of 3C 279 becomes optically thin at short (mm) wavelengths.
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| 3. |
- Kristan, Matej, et al.
(författare)
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The first visual object tracking segmentation VOTS2023 challenge results
- 2023
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Ingår i: 2023 IEEE/CVF International conference on computer vision workshops (ICCVW). - : Institute of Electrical and Electronics Engineers Inc.. - 9798350307443 - 9798350307450 ; , s. 1788-1810
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking Segmentation VOTS2023 challenge is the eleventh annual tracker benchmarking activity of the VOT initiative. This challenge is the first to merge short-term and long-term as well as single-target and multiple-target tracking with segmentation masks as the only target location specification. A new dataset was created; the ground truth has been withheld to prevent overfitting. New performance measures and evaluation protocols have been created along with a new toolkit and an evaluation server. Results of the presented 47 trackers indicate that modern tracking frameworks are well-suited to deal with convergence of short-term and long-term tracking and that multiple and single target tracking can be considered a single problem. A leaderboard, with participating trackers details, the source code, the datasets, and the evaluation kit are publicly available at the challenge website1
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| 4. |
- Svantesson, Eleonor, et al.
(författare)
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Clinical Outcomes After Anterior Cruciate Ligament Injury: Panther Symposium ACL Injury Clinical Outcomes Consensus Group.
- 2020
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Ingår i: Orthopaedic journal of sports medicine. - 2325-9671. ; 8:7
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Tidskriftsartikel (refereegranskat)abstract
- A stringent outcome assessment is a key aspect of establishing evidence-based clinical guidelines for anterior cruciate ligament (ACL) injury treatment. To establish a standardized assessment of clinical outcome after ACL treatment, a consensus meeting including a multidisciplinary group of ACL experts was held at the ACL Consensus Meeting Panther Symposium, Pittsburgh, Pennsylvania, USA, in June 2019. The aim was to establish a consensus on what data should be reported when conducting an ACL outcome study, what specific outcome measurements should be used, and at what follow-up time those outcomes should be assessed. The group reached consensus on 9 statements by using a modified Delphi method. In general, outcomes after ACL treatment can be divided into 4 robust categories: early adverse events, patient-reported outcomes (PROs), ACL graft failure/recurrent ligament disruption, and clinical measures of knee function and structure. A comprehensive assessment after ACL treatment should aim to provide a complete overview of the treatment result, optimally including the various aspects of outcome categories. For most research questions, a minimum follow-up of 2 years with an optimal follow-up rate of 80% is necessary to achieve a comprehensive assessment. This should include clinical examination, any sustained reinjuries, validated knee-specific PROs, and health-related quality of life questionnaires. In the midterm to long-term follow-up, the presence of osteoarthritis should be evaluated. This consensus paper provides practical guidelines for how the aforementioned entities of outcomes should be reported and suggests the preferred tools for a reliable and valid assessment of outcome after ACL treatment.
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| 5. |
- Tang, Siyang, et al.
(författare)
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Hybrid Transceiver Design for Beamspace MIMO-NOMA in Code-Domain for MmWave Communication Using Lens Antenna Array
- 2020
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Ingår i: IEEE Journal on Selected Areas in Communications. - : Institute of Electrical and Electronics Engineers (IEEE). - 0733-8716 .- 1558-0008. ; 38:9, s. 2118-2127
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Tidskriftsartikel (refereegranskat)abstract
- As a hybrid MIMO architecture, beamspace multiple input multiple output (MIMO) can significantly reduce the number of required radio frequency (RF) chains in millimeter wave (mmWave) massive MIMO systems without obvious performance loss, in which, however, the number of users supported cannot be larger than that of RF chains. To break this fundamental limit, we introduce the concept of code-domain non-orthogonal multiple access (NOMA) into beamspace MIMO. A beam selection scheme is proposed first to maximize the sum-rate by utilizing the quasi-orthogonality of the beamspace channel. Furthermore, a low-complexity detection algorithm is developed to realize the transceiver design in mmWave communications using lens antennas. Finally, numerical results of the decoding complexity at receiver side are analyzed. Simulation results show that the proposed beamspace MIMO-NOMA in code domain can achieve higher spectrum and energy efficiency compared with the existing beamspace MIMO.
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| 6. |
- Xiao, Yue, et al.
(författare)
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Fully Decentralized Federated Learning-Based On-Board Mission for UAV Swarm System
- 2021
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Ingår i: IEEE Communications Letters. - : Institute of Electrical and Electronics Engineers (IEEE). - 1089-7798 .- 1558-2558. ; 25:10, s. 3296-3300
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Tidskriftsartikel (refereegranskat)abstract
- To handle the data explosion in the era of Internet-of-things, it is of interest to investigate the decentralized network, with the aim at relaxing the burden at the central server along with preserving data privacy. In this work, we develop a fully decentralized federated learning (FL) framework with an inexact stochastic parallel random walk alternating direction method of multipliers (ISPW-ADMM). Performing more efficient communication and enhanced privacy preservation compared with the current state-of-the-art, the proposed ISPW-ADMM can be partially immune to the effect of time-varying dynamic network and stochastic data collection, while still in fast convergence. Benefiting from the stochastic gradients and biased first-order moment estimation, the proposed framework can be applied to any decentralized FL tasks over time-varying graphs. Thus, to demonstrate the practicability of such a framework in providing fast convergence, high communication efficiency, noise robustness for a specific on-board mission to some extent, we study the extreme learning machine-based FL model beamforming design in unmanned aerial vehicle communications, as verified by the numerical simulations.
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| 7. |
- Ye, Yu, et al.
(författare)
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Decentralized Consensus Optimization Based on Parallel Random Walk
- 2020
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Ingår i: IEEE Communications Letters. - : Institute of Electrical and Electronics Engineers (IEEE). - 1089-7798 .- 1558-2558. ; 24:2, s. 391-395
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Tidskriftsartikel (refereegranskat)abstract
- The alternating direction method of multipliers (ADMM) has recently been recognized as a promising approach for large-scale machine learning models. However, very few results study ADMM from the aspect of communication costs, especially jointly with running time. In this letter, we investigate the communication efficiency and running time of ADMM in solving the consensus optimization problem over decentralized networks. We first review the effort of random walk ADMM (W-ADMM), which reduces communication costs at the expense of running time. To accelerate the convergence speed of W-ADMM, we propose the parallel random walk ADMM (PW-ADMM) algorithm, where multiple random walks are active at the same time. Moreover, to further reduce the running time of PW-ADMM, the intelligent parallel random walk ADMM (IPW-ADMM) algorithm is proposed through integrating the Random Walk with Choice with PW-ADMM. By numerical results from simulations, we demonstrate that the proposed algorithms can be both communication efficient and fast in running speed compared with state-of-the-art methods.
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