| 1. |
- Andersson, Helene, et al.
(författare)
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Microfabrication and microfluidics for tissue engineering : state of the art and future opportunities
- 2004
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Ingår i: Lab on a Chip. - : Royal Society of Chemistry (RSC). - 1473-0197 .- 1473-0189. ; 4:2, s. 98-103
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Forskningsöversikt (refereegranskat)abstract
- An introductory overview of the use of microfluidic devices for tissue engineering is presented. After a brief description of the background of tissue engineering, different application areas of microfluidic devices are examined. Among these are methods for patterning cells, topographical control over cells and tissues, and bioreactors. Examples where microfluidic devices have been employed are presented such as basal lamina, vascular tissue, liver, bone, cartilage and neurons. It is concluded that until today, microfluidic devices have not been used extensively in tissue engineering. Major contributions are expected in two areas. The first is growth of complex tissue, where microfluidic structures ensure a steady blood supply, thereby circumventing the well-known problem of providing larger tissue structures with a continuous flow of oxygen and nutrition, and withdrawal of waste products. The second, and probably more important function of microfluidics, combined with micro/nanotechnology, lies in the development of in vitro physiological systems for studying fundamental biological phenomena.
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| 2. |
- Andersson, Helene, et al.
(författare)
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Microfluidic devices for cellomics : a review
- 2003
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Ingår i: Sensors and actuators. B, Chemical. - : Elsevier BV. - 0925-4005 .- 1873-3077. ; 92:3, s. 315-325
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Forskningsöversikt (refereegranskat)abstract
- A review of microfluidic devices for cellomics is presented. After a brief description of the historical background of Lab-on-Chip (LOC) devices, different areas are reviewed. Devices for cell sampling are presented, followed by cell trapping and cell sorting devices based upon mechanical and electrical principles. Subsequently, a popular type of cell sorters, flow cytometers, is considered, followed by a chapter describing devices for cell treatment: cell lysis, poration/gene transfection and cell fusion devices. Finally a number of microfluidic devices for cellular studies are reviewed. The large amount of very recent publications treated in this review indicates the rapidly growing interest in this exciting application area of LOC.
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| 3. |
- Andersson, Helene, et al.
(författare)
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Microtechnologies and nanotechnologies for single-cell analysis
- 2004
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Ingår i: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 15:1, s. 44-49
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Forskningsöversikt (refereegranskat)abstract
- Many efforts are currently underway to try and mimic the properties of single cells with the aim of designing chips that are as efficient as cells. However, cells are nature's nanotechnology engineering at the scale of atoms and molecules, and it might be better to envision a microchip that utilizes a single cell as an experimentation platform. A novel, so-called laboratory-in-a-cell concept has been described, where advantage is taken of micro-and nanotechnological tools to enable precise control of the biochemical cellular environment; these tools also offer the possibility to analyse the composition of single cells. Methods for single-cell handling and analysis are being developed and will be required for this concept to progress further.
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| 4. |
- Emmelkamp, J., et al.
(författare)
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The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems
- 2004
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Ingår i: Electrophoresis. - : Wiley. - 0173-0835 .- 1522-2683. ; 25:21-22, s. 3740-3745
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Tidskriftsartikel (refereegranskat)abstract
- A novel method for studying unlabeled living mammalian cells based on their autofluorescence (AF) signal in a prototype microfluidic device is presented. When combined, cellular AF detection and microfluidic devices have the potential to facilitate high-throughput analysis of different cell populations. To demonstrate this, unlabeled cultured cells in microfluidic devices were excited with a 488 nm excitation light and the AF emission (> 505 nm) was detected using a confocal fluorescence microscope (CFM). For example, a simple microfluidic three-port glass microstructure was used together with conventional electroosmotic flow (EOF) to switch the direction of the fluid flow. As a means to test the potential of AF-based cell sorting in this microfluidic device, granulocytes were successfully differentiated from human red blood cells (RBCs) based on differences in AF This study demonstrated the use of a simple microfabricated device to perform high-throughput live cell detection and differentiation without the need for cell-specific fluorescent labeling dyes and thereby reducing the sample preparation time. Hence, the combined use of microfluidic devices and cell AF may have many applications in single-cell analysis.
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| 5. |
- Wolbers, F., et al.
(författare)
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Apoptosis induced kinetic changes in autofluorescence of cultured HL60 cells-possible application for single cell analysis on chip
- 2004
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Ingår i: Apoptosis (London). - 1360-8185 .- 1573-675X. ; 9:6, s. 749-755
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Tidskriftsartikel (refereegranskat)abstract
- Introduction: This paper presents a new method using natural cellular fluorescence (autofluorescence, AF) to study apoptosis. Measurement of AF reduces sample preparation time and avoids cellular toxicity due to the fact that no labelling is required. Methods: Human promyelocytic leukemic HL60 cells were incubated with camptothecin (CPT), tumour necrosis factor (TNF)-alpha in combination with cycloheximide (CHX), or irradiated with 6 or 10 Gray, during varying time periods, to initiate apoptosis. AF was measured at the flow cytometer. Results: Induction of apoptosis results in the shrinkage of the cell and the fragmentation into apoptotic bodies. With flow cytometry, 4 subpopulations, viable, early apoptotic, late apoptotic and the necrotic cells, can be distinguished. Induction of apoptosis results in a decrease in AF intensity compared to untreated HL60 cells, especially seen in the late apoptotic subpopulation. The AF intensity is found to decrease significantly in time (between 2 h and 24 h) for all the four apoptotic inducers used. Conclusions: Our results show that it is possible to specifically measure the apoptotic-induced kinetic changes in AF in HL60 cells. A decrease in AF intensity is seen from 2 h till 24 h. These results open a door for future developments in single-cell analysis.
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