| 1. |
- Bratlie, Svein-Olav, et al.
(författare)
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The renin–angiotensin system in Barrett’s esophagus
- 2016
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Ingår i: Scandinavian Journal of Gastroenterology. - : Informa UK Limited. - 0036-5521 .- 1502-7708. ; 51:9978, s. 1037-1042
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Tidskriftsartikel (refereegranskat)abstract
- ABSTRACT Objective: Barrett’s esophagus (BE) is a risk factor for esophageal adenocarcinoma. In addition to its classical endocrine character known for hemodynamic regulation, the renin–angiotensin system (RAS) can be associated with inflammation, wound healing, and cancer. The aim of this study was to explore a potential expression of the RAS in BE, with or without the presence of dysplasia. Material and methods: Biopsy material was prepared for western blotting and immunohistochemistry. Non-BE patients (controls) were compared with BE patients regarding RAS in the squamous epithelium. In the columnar BE mucosa, RAS expression was studied in patients with and without dysplasia. Key components of the ‘classical’ RAS were assessed: the angiotensin-converting enzyme (ACE) and the angiotensin II subtype 1 and 2 receptors (AT1R and AT2R). Results: The presence of RAS factors was confirmed in the esophageal mucosa of both control and BE patients. ACE protein expression was 48% lower (p1⁄40.001) whereas AT1R was 45% higher (p1⁄40.039) in the squamous epithelium of BE patients compared to epithelia from non-BE controls. In the meta- plastic intestinal-like epithelium, AT1R expression was 37% higher in BE patients with confirmed dyspla- sia than in patients without dysplasia (p 1⁄4 0.009). Immunohistochemistry showed an altered distribution of RAS proteins in BE patients with dysplasia. Conclusions: The differential RAS expression observed may prove to be useful as a biomarker or a pharmaceutical target.
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| 2. |
- Casselbrant, Anna, 1970, et al.
(författare)
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Effects of fixation on electrophysiology and structure of human jejunal villi
- 2018
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Ingår i: Microscopy Research and Technique. - : Wiley. - 1059-910X. ; 81:4, s. 376-383
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Tidskriftsartikel (refereegranskat)abstract
- The villi of human jejunum vary in size and shape during different functional conditions. In the base the lamina propria is isotonic with blood, in the tip hyperosmotic. Here we study electrophysiological and morphological effects of incubation in hypotonic, isotonic, or hypertonic solutions, and to test various isotonic fixatives for microscopy. Samples of jejunal mucosae, obtained during surgery in obese patients, were studied in Ussing chambers where electrical parameters were registered during incubation in Krebs solution at various osmolarities, and during fixation in formaldehyde, glutaraldehyde, or osmium tetroxide (OsO4). The same fixatives were used for other jejunal specimens that were fixed directly for light microscopy. Morphometry was carried out to determine size and height of villi, proportion of lamina propria, and surface enlargement due to villi. Ussing chamber incubation in fluids with low osmolarity resulted in increased electrical resistance and epithelial swelling. Opposite results were obtained at high osmolality. Fixation was faster in formaldehyde than in glutaraldehyde or OsO4. In biopsies processed directly for light microscopy the proportions of lamina propria of the mucosa, and of lamina propria of villi, were significantly larger in biopsies fixed in formaldehyde than after fixation in glutaraldehyde or OsO4. The villus tips sometimes ended with a bleb with prominent spaces between the epithelial cells. In summary, jejunal villi swell in vitro when exposed to hypotonic solutions, and shrink in hypertonic solutions. Much of the morphological changes occurring during fixation can be related to the physiological hyperosmolar milieu in villus tips.
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| 3. |
- Potipat, J., et al.
(författare)
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Bioconcentration factor (BCF) and depuration of heavy metals of oysters (Saccostrea cucullata) and mussels (Perna viridis) in the river basins of coastal area of Chanthaburi Province, Gulf of Thailand
- 2015
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Ingår i: EnvironmentAsia. - 2586-8861. ; 8:2, s. 118-128
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Tidskriftsartikel (refereegranskat)abstract
- This study investigated the distribution of Pb, Cd, Cr, Fe, Cu and Zn in the whole tissues of oysters (Saccostrea cucullata) and mussels (Perna viridis) collected from the three river basins of the coastal area in Chanthaburi Province, Thailand. The heavy metal concentrations in oysters followed the order: Zn>Fe>Cu>Cd>Cr>Pb, whereas in the mussels it was: Fe>Zn>Cu>Cr>Cd>Pb. The concentrations of Cu and Zn in the oysters were higher than the permission standard, and the bioconcentration factor (BCF) study revealed the ability of the oysters to accumulate the heavy metals. The results of depuration found that Cu and Zn levels in both oysters and mussels were below the permission standard after the end of the process. The time of depuration was related to the levels of Cu and Zn with the higher r values and the relationship of the depuration study obtained from the results of multiple regression analysis.
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