| 1. |
- Ahlén, Gustaf, et al.
(författare)
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Mannosylated mucin-type immunoglobulin fusion proteins enhance antigen-specific antibody and T lymphocyte responses
- 2012
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 7:10
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Tidskriftsartikel (refereegranskat)abstract
- Targeting antigens to antigen-presenting cells (APC) improve their immunogenicity and capacity to induce Th1 responses and cytotoxic T lymphocytes (CTL). We have generated a mucin-type immunoglobulin fusion protein (PSGL-1/mIgG2b), which upon expression in the yeast Pichia pastoris became multivalently substituted with O-linked oligomannose structures and bound the macrophage mannose receptor (MMR) and dendritic cell-specific intercellular adhesion molecule-3 grabbing non-integrin (DC-SIGN) with high affinity in vitro. Here, its effects on the humoral and cellular anti-ovalbumin (OVA) responses in C57BL/6 mice are presented.OVA antibody class and subclass responses were determined by ELISA, the generation of anti-OVA CTLs was assessed in 51Cr release assays using in vitro-stimulated immune spleen cells from the different groups of mice as effector cells and OVA peptide-fed RMA-S cells as targets, and evaluation of the type of Th cell response was done by IFN-γ, IL-2, IL-4 and IL-5 ELISpot assays.Immunizations with the OVA − mannosylated PSGL-1/mIgG2b conjugate, especially when combined with the AbISCO®-100 adjuvant, lead to faster, stronger and broader (with regard to IgG subclass) OVA IgG responses, a stronger OVA-specific CTL response and stronger Th1 and Th2 responses than if OVA was used alone or together with AbISCO®-100. Also non-covalent mixing of mannosylated PSGL-1/mIgG2b, OVA and AbISCO®-100 lead to relatively stronger humoral and cellular responses. The O-glycan oligomannoses were necessary because PSGL-1/mIgG2b with mono- and disialyl core 1 structures did not have this effect.Mannosylated mucin-type fusion proteins can be used as versatile APC-targeting molecules for vaccines and as such enhance both humoral and cellular immune responses.
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| 2. |
- Gustafsson, Anki, et al.
(författare)
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Pichia pastoris-produced mucin-type fusion proteins with multivalent O-glycan substitution as targeting molecules for mannose-specific receptors of the immune system
- 2011
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Ingår i: Glycobiology. - : Oxford University Press (OUP). - 0959-6658 .- 1460-2423. ; 21:8, s. 1071-1086
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Tidskriftsartikel (refereegranskat)abstract
- Mannose-binding proteins like the macrophage mannose receptor (MR), the dendritic cell-specific intercellular adhesion molecule-3 grabbing non-integrin (DC-SIGN) and mannose-binding lectin (MBL) play crucial roles in both innate and adaptive immune responses. Immunoglobulin fusion proteins of the P-selectin glycoprotein ligand-1 (PSGL-1/mIgG2b) carrying mostly O-glycans and, as a control, the a1-acid glycoprotein (AGP/mIgG2b) carrying mainly N-linked glycans were stably expressed in the yeast Pichia pastoris. P. pastoris-produced PSGL-1/mIgG2b was shown to carry O-glycans that mediated strong binding to mannose-specific lectins in a lectin array and were susceptible to cleavage by a-mannosidases including an a1,2- but not an a1,6-mannosidase. Electrospray ionization - ion trap mass spectrometry (ESI-MS) confirmed the presence of O-glycans containing up to 9 hexoses with the penta- and hexasaccharides being the predominant ones. a1,2- and a1,3-linked, but not a1,6-linked, mannose residues were detected by 1H-nuclear magnetic resonance (1H-NMR) spectroscopy confirming the results of the mannosidase cleavage. The apparent equilibrium dissociation constants for binding of PNGase F-treated mannosylated PSGL-1/mIgG2b to MR, DC-SIGN and MBL were shown by surface plasmon resonance to be 126, 56 and 16 nM, respectively. In conclusion, PSGL-1/mIgG2b expressed in P. pastoris carried O-glycans mainly comprised of a-linked mannoses and with up to nine residues. It bound mannose-specific receptors with high apparent affinity and may become a potent targeting molecule for these receptors in vivo.
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| 3. |
- Holgersson, Ulrika, et al.
(författare)
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Att leva som kvinna i Lund: från 1862 och framåt
- 2012
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Ingår i: Lunds historia – staden och omlandet. Band 3: Modern tid. Där tankar möts (1862–2010). - 9789198050424 ; 3, s. 340-357
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Bokkapitel (populärvet., debatt m.m.)
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| 4. |
- Holgersson, Ulrika
(författare)
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Herstory revisited
- 2012
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Ingår i: Scandia. - 0036-5483. ; 78:2, suplement, s. 42-50
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Tidskriftsartikel (refereegranskat)abstract
- This text deals with a very particular dilemma: how should I, a post-linguistic-turn gender historian, deal with the reality that women are constantly excluded from the continuing writing of the past (or are silenced in the history that is played out in front of my eyes) without falling back on the herstory tradition that the majority of us academics say we have left behind? The problem is discussed in theoretical terms, but also empirically in relation to two specific situations. The first is a commission to write a history of women and the women’s movement for the Swedish city of Lund. The second is an invitation to address a Social Democratic women’s association on the subject of the Swedish general election campaign of 2010, and more specifically the media resistance shown towards Mona Sahlin, the female candidate for prime minister. With the help of a further example – the play Historia A by the dramatic arts collective Potato Potato, a reaction to the omission of women from Swedish school history textbooks – I will conclude by illustrating the new path I would advocate: a very queer women’s history – Herstory revisited,or Herstory 2.0.
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| 5. |
- Holgersson, Ulrika
(författare)
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Historieämnet mer politiserat
- 2010
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Ingår i: Skånska dagbladet. - 1103-9973.
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 6. |
- Holgersson, Ulrika
(författare)
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Historisk spelfilmsanalys : exemplet Gustaf Molanders Vi som går köksvägen 1932
- 2014
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Ingår i: Scandia. - 0036-5483. ; 80:2, s. 36-65
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Tidskriftsartikel (refereegranskat)abstract
- The aim of this article is to offer an object lesson in how historians could make use of feature film as primary source material if they were to use the analytical tools developed in the field of film studies and by historians who started to work with film in the 1970s and 1980s. My own work on representations of maidservants in Swedish feature films in the 1930s and 1940s is used as a case in point. As a concrete example, I consider the trail- blazing blockbuster Vi som går köksvägen (Gustaf Molander, 1932, ‘Servants’ Entrance’, though not to be confused with the American film of the same name of 1934). In dialogue with the fields of film studies and ”film and history”, I argue for a historically informed film analysis that treats feature film’s form and style, and its ‘re-historicization’, by relating it to the wider context of its production, reception, and the film culture in general. Further, a natural starting-point in any study of the representations of a certain category of people, such as maidservants, would be the different characters in the films in mind. Following the film scholar Richard Dyer, I suggest concentrating in the first instance on how the film stars act in their roles, considering each as a complex intersection between film, film culture, and society as a whole. Again referring to Dyer, I hold that each character captures a certain set of societal principals and values, sifting out types from stereotypes, iden- tifying the ones who represent the overall ideology of the film’s narrative and those who do not. Taking a close look at Vi som går köksvägen, I demonstrate how all these things come together, in an analysis that deepens our understanding of this particular film as a historical source for Swedish society in the 1930s. Paying especial attention to the characters in the film, I discuss various aspects of the film’s form (story, plot, narration, parallelisms) and style (setting, properties, acting, make-up, and lightning), in the course of which I relate to other types of material, shedding light on the film’s production and reception and on the construction of Tutta Rolf as a Swedish film star. I conclude that it is a surprisingly radical film, celebrating the strong new woman who was part of the new modern Sweden.
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| 7. |
- Holgersson, Ulrika
(författare)
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Klass : feministiska och kulturanalytiska perspektiv
- 2011
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Bok (övrigt vetenskapligt/konstnärligt)abstract
- Efter Berlinmurens fall och postmodernismens genomslag inom vetenskapen talades det om klassbegreppets kris, eller rent av dess död. Utomlands fördes en livlig diskussion, men i Sverige var det jämförelsevis tyst. På senare år har det dock blivit på modet igen att beskriva samhället i klasstermer, såväl i den offentliga debatten som i litteraturen och populärkulturen. Det här är den första grundliga läroboken och genomgången på svenska av de senaste decenniernas klassdebatt.
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| 8. |
- Holgersson, Ulrika
(författare)
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Medias bröllopsyra
- 2010
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Ingår i: Skånska dagbladet. - 1103-9973.
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 9. |
- Holgersson, Ulrika, et al.
(författare)
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"Regional Conflicts: From the Merchant's House to the People's Home"
- 2010
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Ingår i: Regional Aesthetics : Locating Swedish Media. - 1654-6601. - 9789188468147 ; Mediehistoriskt arkiv 15, s. 191-211
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- This article is an analysis of the Swedish feature film "Brita i grosshandlarhuset" from 1946 about a poor girl that moves to the city finding employment as a maid in the house of a rich merchant. Here I examine the compromises - in terms of constructions of class and gender - that paved the way for a new narrative, with the progression of imagery from, so to speak, the symbol of the merchant house to the metaphor of the "Folkhem". In special focus stands the relation between city and country side.
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| 11. |
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| 13. |
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| 14. |
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| 15. |
- Sjöblom, Magnus, et al.
(författare)
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Secretion and expression dynamics of a GFP-tagged mucin-type fusion protein in high cell density Pichia pastoris bioreactor cultivations
- 2012
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Ingår i: Advances in Bioscience and Biotechnology. - : Scientific Research Publishing, Inc.. - 2156-8456 .- 2156-8502. ; 3:3, s. 238-248
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Tidskriftsartikel (refereegranskat)abstract
- The methanol inducible alcohol oxidase 1 promoter and the Saccharomyces cerevisiae alpha-factor prepro secretion signal were used to drive expression and secretion of a mucin-type fusion protein by Pichia pastoris in 1 L scale bioreactors. The aim of the study was to understand how varying expression rates influenced the secretion dynamics of the fusion protein in terms of intracellular- and extracellular concentrations. Endoplasmic reticulum (ER) folding stress was assessed by the relative expression of the unfolded protein response controlled KAR2 gene. Three predefined methanol feeding models were applied to control the fusion protein synthesis rate. To track the fusion protein synthesis in a non-invasive manner and to follow its intracellular distribution, its C-terminal was linked to the green fluorescent protein. Under all conditions the fusion protein was found to partially accumulate intracellularly, where the major fraction was an insoluble, fluorescent full-sized protein. The high degree of glycosylation of the insoluble fusion protein indicated a secretory bottle-neck in the Golgi-system. This result was consistent with low ER folding stress as quantified by the relative expression of the KAR2 gene. Reduction of recombinant protein synthesis rate, by using lower feed rates of methanol, enhanced extracellular concentrations from 8 to 18 mg·L–1 and reduced the rate of intracellular accumulation. This clearly demonstrates the importance of tuning the synthesis rate with secretory bottle-necks to maintain secretion.
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